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Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Burkholderia mallei (strain ATCC 23344)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei598Aminoacyl-adenylateUniRule annotation1
Binding sitei642ATPUniRule annotation1
Metal bindingi922ZincUniRule annotation1
Metal bindingi925ZincUniRule annotation1
Metal bindingi942ZincUniRule annotation1
Metal bindingi945ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBMAL243160:G1G3W-11691-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 2UniRule annotation
Short name:
IleRS 2UniRule annotation
Gene namesi
Name:ileS2UniRule annotation
Synonyms:ileS-2
Ordered Locus Names:BMAA0963
OrganismiBurkholderia mallei (strain ATCC 23344)
Taxonomic identifieri243160 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006693 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983691 – 967Isoleucine--tRNA ligase 2Add BLAST967

Proteomic databases

PRIDEiQ62CD5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243160.BMAA0963

Structurei

3D structure databases

ProteinModelPortaliQ62CD5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi639 – 643"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiPSWYIRT

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q62CD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYKETLNL LQTPFPMKGD LPRREPALVE RWAAQRVYAR MRAAAAGRPP
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAV NKVLKDIVLK SRFAAGYDAQ WIPGWDCHGM
110 120 130 140 150
PIEHRIEQLH GRGLPPAAVQ RLCREYAFEQ TERQRRDFLR LGLLGDWPHA
160 170 180 190 200
FRTMDFRTEA NELRFLERIR ARGLLYRGQK PVNWCVDCQS ALAEAELEYA
210 220 230 240 250
RKTSVAIHAG LRVRDPVDFA SRFRRRPVLD KPAMLVVWTT TPWTIPGNAA
260 270 280 290 300
AGVRADAPYG LYDTPGALIV VAQPLADALF ASLGVDHALC ALARGRELVG
310 320 330 340 350
LALGQPFFAG RDVPVVEAEF VTLDAGTGIV HLAPAHGAED AELCRRLGIA
360 370 380 390 400
GENVVDGAGR FAADLPEIGG LPLADGIARI VAKLRADGTL VREAAFEHAY
410 420 430 440 450
PHCWRHKTPI LFRSTPQWFI GMDIECEQGE ADPGRAAVTE EAGATGEARK
460 470 480 490 500
VGKAEEAEEA GPAKTLRASA RDAIADVPFY PPSARQRMEA MIDGRPDWCV
510 520 530 540 550
SRQRTWGVPL PYFVRRDDRS LHPRSARLVE AVAARVERDG IAAWSRLRPA
560 570 580 590 600
ELGVDENAYE KLSDTLDVWF DSGSIHATVY RDAARADTGG YPADLYLEGA
610 620 630 640 650
DQHRGWFGAS LMTGCAADGR APFRAILTHG FVVDGAGRKM SKSLGNTVSP
660 670 680 690 700
QRIADTRGAD ILRLWIASTD YAAEMSISDE ILERVVETYR RMRNTLRFLL
710 720 730 740 750
QNVADFDPRD DAVPAGQLLD VDRYALARCR EFVDACRSAY ARYDFVAVTR
760 770 780 790 800
LAHGYCAEEL GGFYLDALKD RLYASVADGV ERRAAQTALH SVLANLLISI
810 820 830 840 850
APILSFTAEE AWTVFAGDER DSVFLHTWDE HAPPPDDAAL ARWAHVRALR
860 870 880 890 900
PHVTKALEEA RGAALIGRSS EAELVVRAPR DVLDALAPLH GELAAVFIVA
910 920 930 940 950
GVTLETADQI AVAVARTPLA RCERCWRHEP SVAAHASGDA LCARCRHALS
960
RRARAERSEP RAAVGSR
Length:967
Mass (Da):106,372
Last modified:October 25, 2004 - v1
Checksum:i5C51960417CE8A58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000011 Genomic DNA Translation: AAU46160.1
RefSeqiWP_004184520.1, NC_006349.2
YP_105643.1, NC_006349.2

Genome annotation databases

EnsemblBacteriaiAAU46160; AAU46160; BMAA0963
GeneIDi3086173
KEGGibma:BMAA0963
PATRICifig|243160.12.peg.4482

Similar proteinsi

Entry informationi

Entry nameiSYI2_BURMA
AccessioniPrimary (citable) accession number: Q62CD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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