Reviewed, UniProtKB/Swiss-Prot Q629Z7 (ISPH1_BURMA)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1
    EC=1.17.1.2

Gene names

Name:	ispH1
Synonyms:	ispH-1
Ordered Locus Names:	BMAA1962

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 HAMAP MF_00191		PRO_0000128791

Sequences

	Sequence		Length	Mass (Da)
	Q629Z7-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 944BC58A7CBE079B	FASTA	313	33,769
	10 20 30 40 50 60 MRVILAQPRG FCAGVVRAIE IVERALQQNG APVYVRHEIV HNRHVVENLR NKGARFVEEL 70 80 90 100 110 120 DEVPHGAVAI FSAHGVAQTV EQDAQARGLD VLDATCPLVT KVHVQGRQYV AAGRRLILIG 130 140 150 160 170 180 HAGHPEVEGT IGQIPAEVIL VQSEAEVDTL TLPADTPVAY VTQTTLSVDD TRGIIEALQR 190 200 210 220 230 240 RFTDIVGPDT RDICYATQNR QAAVRELSEQ VDVLLVVGAT NSSNSNRLRE IGTESGVPSY 250 260 270 280 290 300 LVADGSEVRA EWFANARTVG LTAGASAPEE MVEDVIAALR ALGPLEVATM SGREEKVEFK 310 LPAKLTQAVA REV

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000011 Genomic DNA. Translation: AAU45677.1.
RefSeq	YP_106485.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3086216.
GenomeReviews	Gene locus BMAA1962 in contig CP000011_GR.
KEGG	bma:BMAA1962.
TIGR	BMAA1962.
Phylogenomic databases
HOGENOM	Q629Z7.
OMA	REENIEF.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_A1962-MON.
BRENDA	1.17.1.2. 260531.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH1_BURMA

Accession

Primary (citable) accession number: Q629Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)