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Q62975 (ZPI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein Z-dependent protease inhibitor
Short name=PZ-dependent protease inhibitor
Short name=PZI
Alternative name(s):
Regeneration-associated serpin 1
Short name=RASP-1
Serpin A10
Gene names
Name:Serpina10
Synonyms:Rasp1, Zpi
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed by the liver and secreted in plasma. Ref.1

Developmental stage

In regenerating livers, it showed maximal expression 48 hours after hepatectomy, expression remaining elevated up to 2 weeks after liver removal. Ref.1

Miscellaneous

Heparin acts as an important cofactor, producing 20 to 100-fold accelerations of SERPINA10 reactions with factor Xa and factor XIa By similarity.

Sequence similarities

Belongs to the serpin family.

Ontologies

Keywords
   Biological processBlood coagulation
Hemostasis
   Cellular componentSecreted
   DomainSignal
   LigandHeparin-binding
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

tissue regeneration

Inferred from expression pattern Ref.1. Source: RGD

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 436416Protein Z-dependent protease inhibitor
PRO_0000032485

Regions

Region128 – 14518Heparin-binding By similarity

Sites

Site2531Essential for interaction with PROZ By similarity
Site3061Essential for interaction with PROZ By similarity
Site400 – 4012Reactive bond By similarity

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation1891N-linked (GlcNAc...) Potential
Glycosylation2871N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict281S → T in AAC52624. Ref.1
Sequence conflict4221Q → R in AAC52624. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q62975 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: 4AAE8B3DB9F520CE

FASTA43650,244
        10         20         30         40         50         60 
MRVVSSLFLP VLLAEVWLVS SFNLSSHSPE APIRLVSQDY ENQTWEEYEW ADPRDDNEYW 

        70         80         90        100        110        120 
LRASQQLSNE TSSFGFSLLR KISMRHDGNV IFSPFGLSVA MVNLMLGAKG ETKVQVENGL 

       130        140        150        160        170        180 
NLQALSQAGP LILPALFKRV KETFSSNKKL GLTQGSFAFI HKDFEIKKTY FNLSTMYFDT 

       190        200        210        220        230        240 
EYVPTNFRNS SQARGLMNHY INKETEGKIP KLFDEINPET KLILVDYILF KGKWLTPFDP 

       250        260        270        280        290        300 
IFTEADTFHL DKYKAVKVPM MYREGNFAST FDKKFRCHIL KLPYQGNATM LVVLMEKSGD 

       310        320        330        340        350        360 
HLALEDYLTT DLVEMWLQDM KTRKMEVFFP KFKLNQRYEM HELLKQVGIR RIFSTSADLS 

       370        380        390        400        410        420 
ELSAVARNLQ VSKVVQQSVL EVDERGTEVV SGTVSEITAY CMPPVIKVDR PFHFIIYEEM 

       430 
SQMLLFLGRV VNPTVL

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of rasp-1, a novel gene encoding a plasma protein associated with liver regeneration."
New L., Liu K., Kamali V., Plowman G., Naughton B.A., Purchio A.F.
Biochem. Biophys. Res. Commun. 223:404-412(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: Long Evans.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U55765 mRNA. Translation: AAC52624.1.
BC088113 mRNA. Translation: AAH88113.1.
IPIIPI00210340.
PIRJC4841.
RefSeqNP_598301.2. NM_133617.2.
XP_003750275.1. XM_003750227.1.
UniGeneRn.10502.

3D structure databases

ProteinModelPortalQ62975.
ModBaseSearch...

Protein family/group databases

MEROPSI04.005.

Proteomic databases

PaxDbQ62975.
PRIDEQ62975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000073709; ENSRNOP00000064104; ENSRNOG00000050761.
ENSRNOT00000075378; ENSRNOP00000066602; ENSRNOG00000048988.
GeneID100909524.
171154.
KEGGrno:100909524.
rno:171154.
UCSCRGD:621220. rat.

Organism-specific databases

CTD51156.
RGD621220. Serpina10.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00560000076649.
HOGENOMHOG000238519.
HOVERGENHBG101138.
OrthoDBEOG4VT5X7.

Gene expression databases

GenevestigatorQ62975.
GermOnlineENSRNOG00000028888. Rattus norvegicus.

Family and domain databases

InterProIPR000295. Prot_inh_Lserp2.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSPR00780. LEUSERPINII.
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. Prot_inh_serpin. 1 hit.
PROSITEPS00284. SERPIN. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio621988.

Entry information

Entry nameZPI_RAT
AccessionPrimary (citable) accession number: Q62975
Secondary accession number(s): Q5M8C2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 5, 2005
Last modified: May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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