Q62969 (PTGIS_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostacyclin synthase
EC=5.3.99.4

Alternative name(s):
Prostaglandin I2 synthase

Gene names

Name:	Ptgis
Synonyms:	Cyp8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2) By similarity.
Catalytic activity	(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Prostaglandin biosynthesis Prostaglandin metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to hypoxia Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interleukin-1 Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interleukin-6 Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of nitric oxide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of angiogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of peroxisome proliferator activated receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB prostaglandin biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	caveola Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 15684702. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro prostaglandin-I synthase activity Inferred from mutant phenotype PubMed 10359560. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Prostacyclin synthase		PRO_0000051912
Regions
	Transmembrane	1 – 21	21	Helical; Potential
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62969 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D2D85ADF3C464863	FASTA	501	57,128
	10 20 30 40 50 60 MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGKD AASFLTRMKE 70 80 90 100 110 120 KHGDIFTVLV GGRYVTVLLD PHSYDTVVWD LRTRLDFHPY AIFLMERIFD LQLPNFNPSE 130 140 150 160 170 180 EKARMKPTLM HKDLQALTEA MYTNLRTVLL GDSTEGGSGW QEKGLLEFSY SSLLSAGYLT 190 200 210 220 230 240 LYGVEASPRT HESQALDRDH SADVFRTFRQ LDLMLPKLAR GSLSVGDKDH ACSVKSRLWK 250 260 270 280 290 300 LLSPAGLASR ADRSSWLESY LRHLEEMGVS EDMQARALVL QLWATQGNMG PTAFWLLLFL 310 320 330 340 350 360 LKNPEALDAV HAELKRIVWQ AEKPVLQMTA LPQKILDSMP VLDSVLNETL RLTAAPFITR 370 380 390 400 410 420 EVMADLALPM ADRREFSLRR GDRLLLFPFL SPQKDPEIYT EPEVFKYNRF LNPDGSEKKD 430 440 450 460 470 480 FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN SIKQFVVLLL THFDLELVSE DTEVPEFDLS 490 500 RYGFGLMQPE EDVPIRYRTR L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Geraci M.W., Gao B., Shepherd D., Moore M., Vernon J., Miller Y.E., Voelkel N.F. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53855 mRNA. Translation: AAB02322.1. BC061814 mRNA. Translation: AAH61814.1.
IPI	IPI00210318.
RefSeq	NP_113745.1. NM_031557.2.
UniGene	Rn.73051.
3D structure databases
ProteinModelPortal	Q62969.
SMR	Q62969. Positions 24-500.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000045949.
PTM databases
PhosphoSite	Q62969.
Proteomic databases
PRIDE	Q62969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25527.
KEGG	rno:25527.
UCSC	RGD:3438. rat.
Organism-specific databases
CTD	5740.
RGD	3438. Ptgis.
Phylogenomic databases
eggNOG	NOG288195.
HOGENOM	HOG000231026.
HOVERGEN	HBG051100.
InParanoid	Q62969.
KO	K01831.
Enzyme and pathway databases
BRENDA	5.3.99.4. 5301.
Gene expression databases
ArrayExpress	Q62969.
Genevestigator	Q62969.
GermOnline	ENSRNOG00000008245. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR024204. Cyt_P450_CYP7A1-type. IPR002403. Cyt_P450_E_grp-IV. IPR027286. PTGIS. [Graphical view]
Pfam	PF00067. p450. 2 hits. [Graphical view]
PIRSF	PIRSF000047. Cytochrome_CYPVIIA1. 1 hit. PIRSF500628. PTGIS. 1 hit.
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	607011.

Entry information

Entry name

PTGIS_RAT

Accession

Primary (citable) accession number: Q62969

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program