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Protein

Prostacyclin synthase

Gene

Ptgis

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).By similarity

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi442Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi5.3.99.4. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:Ptgis
Synonyms:Cyp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3438. Ptgis.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519121 – 501Prostacyclin synthaseAdd BLAST501

Proteomic databases

PaxDbiQ62969.
PRIDEiQ62969.

PTM databases

PhosphoSitePlusiQ62969.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010891.

Structurei

3D structure databases

ProteinModelPortaliQ62969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ62969.
KOiK01831.
PhylomeDBiQ62969.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view]
PfamiPF00067. p450. 2 hits.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGKD
60 70 80 90 100
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWD LRTRLDFHPY
110 120 130 140 150
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HKDLQALTEA MYTNLRTVLL
160 170 180 190 200
GDSTEGGSGW QEKGLLEFSY SSLLSAGYLT LYGVEASPRT HESQALDRDH
210 220 230 240 250
SADVFRTFRQ LDLMLPKLAR GSLSVGDKDH ACSVKSRLWK LLSPAGLASR
260 270 280 290 300
ADRSSWLESY LRHLEEMGVS EDMQARALVL QLWATQGNMG PTAFWLLLFL
310 320 330 340 350
LKNPEALDAV HAELKRIVWQ AEKPVLQMTA LPQKILDSMP VLDSVLNETL
360 370 380 390 400
RLTAAPFITR EVMADLALPM ADRREFSLRR GDRLLLFPFL SPQKDPEIYT
410 420 430 440 450
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN
460 470 480 490 500
SIKQFVVLLL THFDLELVSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRTR

L
Length:501
Mass (Da):57,128
Last modified:November 1, 1996 - v1
Checksum:iD2D85ADF3C464863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53855 mRNA. Translation: AAB02322.1.
BC061814 mRNA. Translation: AAH61814.1.
RefSeqiNP_113745.1. NM_031557.2.
UniGeneiRn.73051.

Genome annotation databases

GeneIDi25527.
KEGGirno:25527.
UCSCiRGD:3438. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53855 mRNA. Translation: AAB02322.1.
BC061814 mRNA. Translation: AAH61814.1.
RefSeqiNP_113745.1. NM_031557.2.
UniGeneiRn.73051.

3D structure databases

ProteinModelPortaliQ62969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010891.

PTM databases

PhosphoSitePlusiQ62969.

Proteomic databases

PaxDbiQ62969.
PRIDEiQ62969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25527.
KEGGirno:25527.
UCSCiRGD:3438. rat.

Organism-specific databases

CTDi5740.
RGDi3438. Ptgis.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ62969.
KOiK01831.
PhylomeDBiQ62969.

Enzyme and pathway databases

BRENDAi5.3.99.4. 5301.

Miscellaneous databases

PROiQ62969.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR027286. PTGIS.
[Graphical view]
PfamiPF00067. p450. 2 hits.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PIRSF500628. PTGIS. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTGIS_RAT
AccessioniPrimary (citable) accession number: Q62969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.