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Protein

Dihydropyrimidinase-related protein 3

Gene

Dpysl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.1 Publication

Caution

Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.Curated

GO - Molecular functioni

  • chondroitin sulfate binding Source: RGD
  • filamin binding Source: Ensembl
  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: InterPro
  • SH3 domain binding Source: RGD

GO - Biological processi

  • actin crosslink formation Source: UniProtKB
  • actin filament bundle assembly Source: UniProtKB
  • cellular response to cytokine stimulus Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of neuron projection development Source: UniProtKB
  • neuron development Source: RGD
  • positive regulation of filopodium assembly Source: UniProtKB
  • positive regulation of neuron projection development Source: RGD
  • protein homooligomerization Source: UniProtKB
  • response to axon injury Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-RNO-399956 CRMPs in Sema3A signaling

Protein family/group databases

MEROPSiM38.976

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropyrimidinase-related protein 3
Short name:
DRP-3
Alternative name(s):
Collapsin response mediator protein 4
Short name:
CRMP-4
TOAD-64/Ulip/CRMP
TUC-4b
Gene namesi
Name:Dpysl3
Synonyms:Crmp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi2410 Dpysl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659191 – 570Dihydropyrimidinase-related protein 3Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259PhosphoserineBy similarity1
Modified residuei431PhosphotyrosineBy similarity1
Modified residuei499PhosphotyrosineBy similarity1
Modified residuei509PhosphothreonineCombined sources1
Modified residuei514PhosphothreonineCombined sources1
Modified residuei518Phosphoserine; by GSK3By similarity1
Modified residuei522PhosphoserineCombined sources1
Modified residuei536PhosphoserineBy similarity1
Modified residuei539PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1
Modified residuei543PhosphothreonineBy similarity1
Isoform 2 (identifier: Q62952-2)
Modified residuei101PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62952
PRIDEiQ62952

2D gel databases

World-2DPAGE0004:Q62952

PTM databases

iPTMnetiQ62952
PhosphoSitePlusiQ62952

Expressioni

Tissue specificityi

Isoform 2 is expressed in growth cones. Up-regulated in motor neurons during axonal regeneration after sciatic nerve lesion. Overexpression of isoform 2 increased neurite extension and branching. Isoform 1 is expressed throughout neurons and their axons.1 Publication

Developmental stagei

At E17, isoform 2 is expressed in the CNS and PNS. Isoform 2 is expressed at high levels in the dorsal root ganglia, trigeminal ganglia, and throughout the spinal cord with the highest level in the ventral horn.1 Publication

Gene expression databases

BgeeiENSRNOG00000018992
GenevisibleiQ62952 RN

Interactioni

Subunit structurei

Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5. Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin.2 Publications

GO - Molecular functioni

  • filamin binding Source: Ensembl
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi247453, 1 interactor
IntActiQ62952, 3 interactors
STRINGi10116.ENSRNOP00000025880

Structurei

3D structure databases

ProteinModelPortaliQ62952
SMRiQ62952
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2584 Eukaryota
COG0044 LUCA
GeneTreeiENSGT00760000119241
HOGENOMiHOG000219145
HOVERGENiHBG000806
InParanoidiQ62952
OMAiYVCSPPP
OrthoDBiEOG091G05F3
PhylomeDBiQ62952
TreeFamiTF314706

Family and domain databases

CDDicd01314 D-HYD, 1 hit
Gene3Di2.30.40.10, 2 hits
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR030628 DRP3
IPR011778 Hydantoinase/dihydroPyrase
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PANTHERiPTHR11647:SF57 PTHR11647:SF57, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR02033 D-hydantoinase, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62952-1) [UniParc]FASTAAdd to basket
Also known as: TUC-4b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI
60 70 80 90 100
VPGGVKTIEA NGKMVMPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG
110 120 130 140 150
TTMIIDHVVP EPESSLTEAY EKWREWADGK SCCDYALHVD ITHWNDSVKQ
160 170 180 190 200
EVQNLSKEKG VNSFMVYMAY KDLYQVSNTE LYEIFTCLGE LGAIAQVHAE
210 220 230 240 250
NGDIIAQEQA RMLEMGITGP EGHVLSRPEE LEAEAVFRAI TVASQTNCPL
260 270 280 290 300
YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA
310 320 330 340 350
FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA
360 370 380 390 400
IPEGTNGVEE RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR
410 420 430 440 450
IAVGSDSDLV IWDPDAVKIV SAKNHQSVAE YNIFEGMELR GAPLVVICQG
460 470 480 490 500
KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK RIKARRKMAD LHAVPRGMYD
510 520 530 540 550
GPVFDLTTTP KGGTPAGSTR GSPTRPNPPV RNLHQSGFSL SGTQVDEGVR
560 570
SASKRIVAPP GGRSNITSLS
Length:570
Mass (Da):61,967
Last modified:December 6, 2005 - v2
Checksum:i4EF1981CDE9A8708
GO
Isoform 2 (identifier: Q62952-2) [UniParc]FASTAAdd to basket
Also known as: TUC-4a

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MSYQGKKNIPRIT → MASGRRGWDS...VLQNLGPKDK

Show »
Length:683
Mass (Da):73,899
Checksum:iBB5C47CCC9253A0D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0165571 – 13MSYQG…IPRIT → MASGRRGWDSSHEDDLPVYL ARPGTTDQVPRQKYGGMFCN VEGAFESKTLDFDALSVGQR GAKTPRSSQGSGRGAGNRPG VEGDTRRGPGREESREPVPE SPKPAGVEIRSATGKEVLQN LGPKDK in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF398465 mRNA Translation: AAM73758.1
AF389425 mRNA Translation: AAK64497.1
U52104 mRNA Translation: AAB03282.1
RefSeqiNP_037066.1, NM_012934.1 [Q62952-1]
XP_006254732.1, XM_006254670.3 [Q62952-2]
UniGeneiRn.93365

Genome annotation databases

EnsembliENSRNOT00000025880; ENSRNOP00000025880; ENSRNOG00000018992 [Q62952-2]
ENSRNOT00000087876; ENSRNOP00000070679; ENSRNOG00000018992 [Q62952-1]
GeneIDi25418
KEGGirno:25418
UCSCiRGD:2410 rat [Q62952-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDPYL3_RAT
AccessioniPrimary (citable) accession number: Q62952
Secondary accession number(s): Q8K4H3, Q91XM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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