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Protein

Zinc finger E-box-binding homeobox 1

Gene

Zeb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
DNA bindingi559 – 61860Homeobox; atypicalAdd
BLAST
Zinc fingeri881 – 90323C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri909 – 93123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri937 – 95822C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: RGD
  • double-stranded DNA binding Source: RGD
  • E-box binding Source: UniProtKB
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD
  • zinc ion binding Source: RGD

GO - Biological processi

  • cellular response to transforming growth factor beta stimulus Source: RGD
  • forebrain development Source: RGD
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of neuron differentiation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • response to activity Source: RGD
  • response to nutrient levels Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger E-box-binding homeobox 1
Alternative name(s):
Transcription factor 8
Short name:
TCF-8
Zinc finger homeodomain enhancer-binding protein
Short name:
Zfhep
Gene namesi
Name:Zeb1
Synonyms:Tcf8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3831. Zeb1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11091109Zinc finger E-box-binding homeobox 1PRO_0000047234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311PhosphoserineCombined sources
Modified residuei33 – 331PhosphoserineCombined sources
Modified residuei293 – 2931PhosphoserineBy similarity
Modified residuei302 – 3021PhosphoserineBy similarity
Cross-linki327 – 327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki473 – 473Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki484 – 484Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki495 – 495Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei657 – 6571PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineBy similarity
Modified residuei671 – 6711PhosphoserineBy similarity
Modified residuei678 – 6781PhosphoserineCombined sources
Modified residuei680 – 6801PhosphothreonineBy similarity
Modified residuei682 – 6821PhosphoserineBy similarity
Cross-linki752 – 752Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62947.
PRIDEiQ62947.

PTM databases

iPTMnetiQ62947.
PhosphoSiteiQ62947.

Interactioni

Subunit structurei

Interacts (via N-terminus) with SMARCA4/BRG1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024336.

Structurei

3D structure databases

ProteinModelPortaliQ62947.
SMRiQ62947. Positions 561-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi968 – 1109142Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri150 – 17324C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri180 – 20223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27225C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri881 – 90323C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri909 – 93123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri937 – 95822C2H2-type 7; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3623. Eukaryota.
ENOG410ZFMZ. LUCA.
HOGENOMiHOG000264256.
HOVERGENiHBG004697.
InParanoidiQ62947.
KOiK09299.
PhylomeDBiQ62947.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62947-1) [UniParc]FASTAAdd to basket

Also known as: Zfhep-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADGPRCKRR KQANPRRNNV TNYNTVVEAN SDSDDEDKLH IVEEESVTDA
60 70 80 90 100
ADCEGGVPDD ELPTDQTVLP GGSDRAGSAK NCWQDDVKDD ECDSDAENEQ
110 120 130 140 150
NHDPNVEEFL QQQDTAVIYP EAPEEDQRQG TPEASGHDDN GTPDAFSQLL
160 170 180 190 200
TCPYCDRGYK RFTSLKEHIK YRHEKNEDNF SCSLCSYTFA YRTQLERHMT
210 220 230 240 250
SHKSGREQRH VTQSGGNRKF KCTECGKAFK YKHHLKEHLR IHSGEKPYEC
260 270 280 290 300
PNCKKRFSHS GSYSSHISSK KCISLMPVNG RPRSGLKTSQ CSSPSLSTSP
310 320 330 340 350
GSPTRPQIRQ KIENKPLQEP LSVNQIKTEP VDYEFKPIVV ASGINCSTPL
360 370 380 390 400
QNGVFSGGGQ LQATSSPQGV VQAVVLPTVG LVSPISINLS DIQNVLKVAV
410 420 430 440 450
DGNIIRQVLE NNQASLASKE QEAVSASSIQ QGGHSVISAI SLPLVDQDGT
460 470 480 490 500
TKIIINYSLE QPGQLQVVPQ NLKKENPAPP KSCKSEKSPE DLTVKSEKDK
510 520 530 540 550
SFDGAADEST CLLCEDCPGD LNALPELKHY DPEHPAQPPP PAPATEKPES
560 570 580 590 600
SASSAGNGDL SPSQPPLKNL LSLLKAYYAL NAQPSTEELT KIADSVNLPL
610 620 630 640 650
DVVKKWFEKM QAGQIPGQSL EPPSPGPGSG NIPAKTEEQP QPVDGNEPQE
660 670 680 690 700
DSTRGQSPLK MTSSPVLPVG SAINGSRSCT SSPSPLNLSS ARNPQGYSCV
710 720 730 740 750
SEGTQEEPQV EPLDLSLPKQ QGELLERSTV SSVYQNSVYS VQEEPLNLSC
760 770 780 790 800
AKKEPQKDSC VTDSEPVVNV VPPSANPINI AIPTVTAQLP TIVAIADQNS
810 820 830 840 850
VPCLRALAAN KQTILIPQVA YTYSATVSPA MQEPPVKVIQ PNGNQDERQD
860 870 880 890 900
TSSEGVSVED QNDSDCTPPK KKTRKAENGM YACDLCDKIF QKSSSLLRHK
910 920 930 940 950
YEHTGKRPHE CGICRKAFKH KHHLIEHMRL HSGEKPYQCD KCGKRFSHSG
960 970 980 990 1000
SYSQHMNHRY SYCKRGAEDR DAMEQEDTGP EALPEVLPTE LVGARASPSQ
1010 1020 1030 1040 1050
ADSDERESLT REEDEDSEKE EEEEDKEMEE LQEDKECENP QEEEEEEEEE
1060 1070 1080 1090 1100
EEEEEEEEEE EAEEAEHEAA AAKTGGAVEE EAAQQAGSFQ QKASGSESKR

LSEEKTNEA
Length:1,109
Mass (Da):121,627
Last modified:February 21, 2001 - v2
Checksum:iBEFE291C8795DDA6
GO
Isoform 2 (identifier: Q62947-2) [UniParc]FASTAAdd to basket

Also known as: Zfhep-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Show »
Length:911
Mass (Da):99,233
Checksum:iCF994BB5F2F3B6FC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 198198Missing in isoform 2. CuratedVSP_006881Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51583 mRNA. Translation: AAB17130.1.
U51584 mRNA. Translation: AAB17131.1.
RefSeqiNP_001295194.1. NM_001308265.1. [Q62947-1]
UniGeneiRn.10600.

Genome annotation databases

GeneIDi25705.
KEGGirno:25705.
UCSCiRGD:3831. rat. [Q62947-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51583 mRNA. Translation: AAB17130.1.
U51584 mRNA. Translation: AAB17131.1.
RefSeqiNP_001295194.1. NM_001308265.1. [Q62947-1]
UniGeneiRn.10600.

3D structure databases

ProteinModelPortaliQ62947.
SMRiQ62947. Positions 561-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024336.

PTM databases

iPTMnetiQ62947.
PhosphoSiteiQ62947.

Proteomic databases

PaxDbiQ62947.
PRIDEiQ62947.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25705.
KEGGirno:25705.
UCSCiRGD:3831. rat. [Q62947-1]

Organism-specific databases

CTDi6935.
RGDi3831. Zeb1.

Phylogenomic databases

eggNOGiKOG3623. Eukaryota.
ENOG410ZFMZ. LUCA.
HOGENOMiHOG000264256.
HOVERGENiHBG004697.
InParanoidiQ62947.
KOiK09299.
PhylomeDBiQ62947.

Miscellaneous databases

PROiQ62947.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR008598. Di19_Zn_binding_dom.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF05605. zf-Di19. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Alternative splicing gives rise to two isoforms of Zfhep, a zinc finger/homeodomain protein that binds T3-response elements."
    Cabanillas A.M., Darling D.S.
    DNA Cell Biol. 15:643-651(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-1109, ALTERNATIVE SPLICING.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-33 AND SER-678, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZEB1_RAT
AccessioniPrimary (citable) accession number: Q62947
Secondary accession number(s): Q62948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: January 20, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.