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Q62936 (DLG3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disks large homolog 3
Alternative name(s):
PSD-95/SAP90-related protein 1
Synapse-associated protein 102
Short name=SAP-102
Short name=SAP102
Gene names
Name:Dlg3
Synonyms:Dlgh3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length849 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling By similarity.

Subunit structure

Interacts through its PDZ domains with NETO1 and APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3 By similarity. Interacts through its PDZ domains with GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN2. Interacts with LRFN1 and LRFN4 By similarity. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Sequence similarities

Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainRepeat
SH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processestablishment of planar polarity

Inferred from electronic annotation. Source: Ensembl

establishment or maintenance of epithelial cell apical/basal polarity

Inferred from electronic annotation. Source: Ensembl

negative regulation of phosphatase activity

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentalpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

dendritic shaft

Inferred from direct assay PubMed 12738960. Source: UniProtKB

growth cone

Inferred from direct assay PubMed 12738960. Source: UniProtKB

neuronal cell body

Inferred from direct assay PubMed 12738960. Source: UniProtKB

postsynaptic density

Inferred from direct assay PubMed 12738960. Source: UniProtKB

synapse

Inferred from direct assay PubMed 12738960. Source: UniProtKB

tight junction

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionPDZ domain binding

Inferred from physical interaction PubMed 15024025. Source: RGD

ionotropic glutamate receptor binding

Inferred from physical interaction PubMed 16540568PubMed 16606616. Source: RGD

phosphatase binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein C-terminus binding

Inferred from physical interaction PubMed 11274188PubMed 8702950. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 12738960PubMed 8702950. Source: UniProtKB

protein domain specific binding

Inferred from direct assay Ref.2. Source: RGD

protein phosphatase binding

Inferred from physical interaction PubMed 20410104. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATP2B4P23634-62EBI-349596,EBI-1174437From a different organism.
CRIPTQ9P0215EBI-349596,EBI-946968From a different organism.
DgkiQ810C52EBI-349596,EBI-8523614
DgkzO085604EBI-349596,EBI-8570505
GRIN1Q05586-23EBI-349596,EBI-8286218From a different organism.
GRIN2AQ128795EBI-349596,EBI-7249937From a different organism.
GRIN2BQ132244EBI-349596,EBI-2256942From a different organism.
Grin2bQ009606EBI-349596,EBI-396905
GRIN2CQ149576EBI-349596,EBI-8285963From a different organism.
GRIN2DO153992EBI-349596,EBI-1754030From a different organism.
KCNA1Q094702EBI-349596,EBI-8286599From a different organism.
KCNA4P224594EBI-349596,EBI-631235From a different organism.
Lrfn2Q460M52EBI-349596,EBI-877185

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q62936-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q62936-2)

The sequence of this isoform differs from the canonical sequence as follows:
     627-640: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 849849Disks large homolog 3
PRO_0000094559

Regions

Domain149 – 23587PDZ 1
Domain244 – 33087PDZ 2
Domain404 – 48481PDZ 3
Domain519 – 58971SH3
Domain659 – 834176Guanylate kinase-like

Amino acid modifications

Modified residue7051Phosphotyrosine By similarity

Natural variations

Alternative sequence627 – 64014Missing in isoform Short.
VSP_003151

Secondary structure

.................... 849
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 34DA9C46C7BB96DB

FASTA84993,539
        10         20         30         40         50         60 
MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG YGGYSSQTLP 

        70         80         90        100        110        120 
SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG SGPSWWPECT CTNRDWYEQA 

       130        140        150        160        170        180 
SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI VLERGNSGLG FSIAGGIDNP HVPDDPGIFI 

       190        200        210        220        230        240 
TKIIPGGAAA MDGRLGVNDC VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE 

       250        260        270        280        290        300 
TIMEVNLLKG PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN 

       310        320        330        340        350        360 
NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA LADNHISHNS 

       370        380        390        400        410        420 
SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR EPRKIILHKG STGLGFNIVG 

       430        440        450        460        470        480 
GEDGEGIFVS FILAGGPADL SGELRRGDRI LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV 

       490        500        510        520        530        540 
AQYRPEEYSR FESKIHDLRE QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS 

       550        560        570        580        590        600 
QGLSFSYGDI LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA 

       610        620        630        640        650        660 
RTGMIESNRD FPGLSDDYYG AKNLKGVTSN TSDSESSSKG QEDAILSYEP VTRQEIHYAR 

       670        680        690        700        710        720 
PVIILGPMKD RVNDDLISEF PHKFGSCVPH TTRPRRDNEV DGQDYHFVVS REQMEKDIQD 

       730        740        750        760        770        780 
NKFIEAGQFN DNLYGTSIQS VRAVAERGKH CILDVSGNAI KRLQQAQLYP IAIFIKPKSI 

       790        800        810        820        830        840 
EALMEMNRRQ TYEQANKIFD KAMKLEQEFG EYFTAIVQGD SLEEIYNKIK QIIEDQSGHY 


IWVPSPEKL 

« Hide

Isoform Short [UniParc].

Checksum: 95F2227D2F6A7F81
Show »

FASTA83592,172

References

[1]"SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo."
Mueller B.M., Kistner U., Kindler S., Chung W.J., Kuhlendahl S., Fenster S.D., Lau L.-F., Veh R.W., Huganir R.L., Gundelfinger E.D., Garner C.C.
Neuron 17:255-265(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INTERACTION WITH GRIN2B.
Tissue: Brain.
[2]"SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), INTERACTION WITH DLGAP1; DLGAP2; DLGAP3 AND DLGAP4.
Tissue: Brain.
[3]"SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family."
Kim J.H., Liao D., Lau L.-F., Huganir R.L.
Neuron 20:683-691(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYNGAP1.
[4]"A novel family of adhesion-like molecules that interacts with the NMDA receptor."
Wang C.Y., Chang K., Petralia R.S., Wang Y.X., Seabold G.K., Wenthold R.J.
J. Neurosci. 26:2174-2183(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LRFN2.
[5]"Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis."
Lee H.W., Choi J., Shin H., Kim K., Yang J., Na M., Choi S.Y., Kang G.B., Eom S.H., Kim H., Kim E.
J. Neurosci. 28:14546-14556(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FRMPD4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U50147 mRNA. Translation: AAA93031.1.
U53367 Genomic DNA. Translation: AAB48561.1.
RefSeqNP_113827.1. NM_031639.1. [Q62936-1]
XP_006257144.1. XM_006257082.1. [Q62936-2]
UniGeneRn.10238.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JXTX-ray1.50A/B393-493[»]
ProteinModelPortalQ62936.
SMRQ62936. Positions 144-332, 400-493, 522-849.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248683. 5 interactions.
IntActQ62936. 27 interactions.
MINTMINT-125016.

PTM databases

PhosphoSiteQ62936.

Proteomic databases

PaxDbQ62936.
PRIDEQ62936.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000003741; ENSRNOP00000003741; ENSRNOG00000002767. [Q62936-1]
ENSRNOT00000045082; ENSRNOP00000047310; ENSRNOG00000002767. [Q62936-2]
GeneID58948.
KEGGrno:58948.
UCSCRGD:68423. rat. [Q62936-1]

Organism-specific databases

CTD1741.
RGD68423. Dlg3.

Phylogenomic databases

eggNOGCOG0194.
GeneTreeENSGT00660000095130.
HOGENOMHOG000232102.
HOVERGENHBG107814.
InParanoidQ62936.
KOK12075.
OMAIMEVNLM.
OrthoDBEOG79GT6P.
PhylomeDBQ62936.
TreeFamTF323171.

Gene expression databases

ArrayExpressQ62936.
GenevestigatorQ62936.

Family and domain databases

Gene3D2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProIPR016313. DLG1.
IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR019590. MAGUK_PEST_N.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR23119. PTHR23119. 1 hit.
PfamPF00625. Guanylate_kin. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFPIRSF001741. MAGUK_DLGH. 1 hit.
SMARTSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ62936.
NextBio611562.
PROQ62936.

Entry information

Entry nameDLG3_RAT
AccessionPrimary (citable) accession number: Q62936
Secondary accession number(s): P70547
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references