Disks large homolog 3 - Rattus norvegicus (Rat)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

With

Entry

#Exp.

IntAct

Notes

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Contribute

Send feedback

Read comments (?) or add your own

Q62936 (DLG3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Disks large homolog 3

Alternative name(s):
PSD-95/SAP90-related protein 1
Synapse-associated protein 102
Short name=SAP-102
Short name=SAP102

Gene names

Name:	Dlg3
Synonyms:	Dlgh3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Sequence length	849 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling By similarity.

Subunit structure

Interacts through its PDZ domains with NETO1 and APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3 By similarity. Interacts through its PDZ domains with GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN2. Interacts with LRFN1 and LRFN4 By similarity. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Sequence similarities

Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of planar polarity Inferred from electronic annotation. Source: Compara establishment or maintenance of epithelial cell apical/basal polarity Inferred from electronic annotation. Source: Compara negative regulation of phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara dendritic shaft Inferred from direct assay PubMed 12738960. Source: UniProtKB growth cone Inferred from direct assay PubMed 12738960. Source: UniProtKB neuronal cell body Inferred from direct assay PubMed 12738960. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara postsynaptic density Inferred from direct assay PubMed 12738960. Source: UniProtKB tight junction Inferred from electronic annotation. Source: Compara
Molecular_function	phosphatase binding Inferred from sequence or structural similarity. Source: UniProtKB protein domain specific binding Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

With	Entry	#Exp.	IntAct	Notes
ATP2B4	P23634-6	2	EBI-349596,EBI-1174437	From a different organism.
Lrfn2	Q460M5	2	EBI-349596,EBI-877185

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 849

849

Disks large homolog 3

PRO_0000094559

Domain

149 – 235

PDZ 1

Domain

244 – 330

PDZ 2

Domain

404 – 484

PDZ 3

Domain

519 – 589

SH3

Domain

659 – 834

176

Guanylate kinase-like

Modified residue

705

Phosphotyrosine By similarity

Alternative sequence

627 – 640

Missing in isoform Short.

VSP_003151

849

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform Long [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 34DA9C46C7BB96DB Show »	FASTA	849	93,539
10 20 30 40 50 60 MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG YGGYSSQTLP 70 80 90 100 110 120 SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG SGPSWWPECT CTNRDWYEQA 130 140 150 160 170 180 SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI VLERGNSGLG FSIAGGIDNP HVPDDPGIFI 190 200 210 220 230 240 TKIIPGGAAA MDGRLGVNDC VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE 250 260 270 280 290 300 TIMEVNLLKG PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN 310 320 330 340 350 360 NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA LADNHISHNS 370 380 390 400 410 420 SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR EPRKIILHKG STGLGFNIVG 430 440 450 460 470 480 GEDGEGIFVS FILAGGPADL SGELRRGDRI LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV 490 500 510 520 530 540 AQYRPEEYSR FESKIHDLRE QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS 550 560 570 580 590 600 QGLSFSYGDI LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA 610 620 630 640 650 660 RTGMIESNRD FPGLSDDYYG AKNLKGVTSN TSDSESSSKG QEDAILSYEP VTRQEIHYAR 670 680 690 700 710 720 PVIILGPMKD RVNDDLISEF PHKFGSCVPH TTRPRRDNEV DGQDYHFVVS REQMEKDIQD 730 740 750 760 770 780 NKFIEAGQFN DNLYGTSIQS VRAVAERGKH CILDVSGNAI KRLQQAQLYP IAIFIKPKSI 790 800 810 820 830 840 EALMEMNRRQ TYEQANKIFD KAMKLEQEFG EYFTAIVQGD SLEEIYNKIK QIIEDQSGHY IWVPSPEKL « Hide
Isoform Short [UniParc]. Checksum: 95F2227D2F6A7F81 Show »	FASTA	835	92,172
10 20 30 40 50 60 MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG YGGYSSQTLP 70 80 90 100 110 120 SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG SGPSWWPECT CTNRDWYEQA 130 140 150 160 170 180 SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI VLERGNSGLG FSIAGGIDNP HVPDDPGIFI 190 200 210 220 230 240 TKIIPGGAAA MDGRLGVNDC VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE 250 260 270 280 290 300 TIMEVNLLKG PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN 310 320 330 340 350 360 NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA LADNHISHNS 370 380 390 400 410 420 SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR EPRKIILHKG STGLGFNIVG 430 440 450 460 470 480 GEDGEGIFVS FILAGGPADL SGELRRGDRI LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV 490 500 510 520 530 540 AQYRPEEYSR FESKIHDLRE QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS 550 560 570 580 590 600 QGLSFSYGDI LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA 610 620 630 640 650 660 RTGMIESNRD FPGLSDDYYG AKNLKGQEDA ILSYEPVTRQ EIHYARPVII LGPMKDRVND 670 680 690 700 710 720 DLISEFPHKF GSCVPHTTRP RRDNEVDGQD YHFVVSREQM EKDIQDNKFI EAGQFNDNLY 730 740 750 760 770 780 GTSIQSVRAV AERGKHCILD VSGNAIKRLQ QAQLYPIAIF IKPKSIEALM EMNRRQTYEQ 790 800 810 820 830 ANKIFDKAMK LEQEFGEYFT AIVQGDSLEE IYNKIKQIIE DQSGHYIWVP SPEKL « Hide

[1]	"SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo." Mueller B.M., Kistner U., Kindler S., Chung W.J., Kuhlendahl S., Fenster S.D., Lau L.-F., Veh R.W., Huganir R.L., Gundelfinger E.D., Garner C.C. Neuron 17:255-265(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INTERACTION WITH GRIN2B. Tissue: Brain.
[2]	"SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density." Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), INTERACTION WITH DLGAP1; DLGAP2; DLGAP3 AND DLGAP4. Tissue: Brain.
[3]	"SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family." Kim J.H., Liao D., Lau L.-F., Huganir R.L. Neuron 20:683-691(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SYNGAP1.
[4]	"A novel family of adhesion-like molecules that interacts with the NMDA receptor." Wang C.Y., Chang K., Petralia R.S., Wang Y.X., Seabold G.K., Wenthold R.J. J. Neurosci. 26:2174-2183(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LRFN2.
[5]	"Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis." Lee H.W., Choi J., Shin H., Kim K., Yang J., Na M., Choi S.Y., Kang G.B., Eom S.H., Kim H., Kim E. J. Neurosci. 28:14546-14556(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FRMPD4.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

U50147 mRNA. Translation: AAA93031.1.
U53367 Genomic DNA. Translation: AAB48561.1.

IPI

IPI00210225.
IPI00230936.

RefSeq

NP_113827.1. NM_031639.1.

UniGene

Rn.10238.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3JXT	X-ray	1.50	A/B	393-493	[»]

ProteinModelPortal

Q62936.

SMR

Q62936. Positions 144-332, 400-493, 522-849.

ModBase

Search...

IntAct

Q62936. 8 interactions.

MINT

MINT-125016.

PhosphoSite

Q62936.

PaxDb

Q62936.

PRIDE

Q62936.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENSRNOT00000003741; ENSRNOP00000003741; ENSRNOG00000002767.
ENSRNOT00000045082; ENSRNOP00000047310; ENSRNOG00000002767.

GeneID

58948.

KEGG

rno:58948.

UCSC

RGD:68423. rat.

CTD

1741.

RGD

68423. Dlg3.

eggNOG

COG0194.

GeneTree

ENSGT00660000095130.

HOGENOM

HOG000232102.

HOVERGEN

HBG107814.

InParanoid

Q62936.

K12075.

OMA

NLMKGPK.

OrthoDB

EOG447FSN.

ArrayExpress

Q62936.

Genevestigator

Q62936.

GermOnline

ENSRNOG00000002767. Rattus norvegicus.

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylate_kin/L-typ_Ca_channel.
IPR020590. Guanylate_kinase_CS.
IPR016313. M-assoc_guanylate_kinase.
IPR019590. MAGUK_PEST_N.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00625. Guanylate_kin. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PIRSF

PIRSF001741. MAGUK_DLGH. 1 hit.

SMART

SM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 3 hits.
SSF50044. SH3. 1 hit.

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

EvolutionaryTrace

Q62936.

NextBio

611562.

Entry name

DLG3_RAT

Accession

Primary (citable) accession number: Q62936
Secondary accession number(s): P70547

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q62936-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q62936-2)

The sequence of this isoform differs from the canonical sequence as follows:
627-640: Missing.