Reviewed, UniProtKB/Swiss-Prot Q62936 (DLG3_RAT)

Names and origin

Protein names

Recommended name:
    Disks large homolog 3
Alternative name(s):
    Synapse-associated protein 102
      Short name=SAP102
    PSD-95/SAP90-related protein 1

Gene names

Name:	Dlg3
Synonyms:	Dlgh3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	849 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling By similarity.
Subunit structure	Interacts through its PDZ domains with APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3 By similarity. Interacts through its PDZ domains with GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4.
Sequence similarities	Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cell soma Inferred from direct assay. Source: UniProtKB dendritic shaft Inferred from direct assay. Source: UniProtKB growth cone Inferred from direct assay. Source: UniProtKB postsynaptic density Inferred from direct assay. Source: UniProtKB
Molecular function	PDZ domain binding Inferred from physical interaction. Source: RGD ionotropic glutamate receptor binding Inferred from physical interaction. Source: RGD protein C-terminus binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATP2B4	P23634-6	2	EBI-349596,EBI-1174437	From a different organism.
Lrfn2	Q460M5	1	EBI-349596,EBI-877185

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q62936-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q62936-2)

The sequence of this isoform differs from the canonical sequence as follows:
     627-640: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 849	849	Disks large homolog 3		PRO_0000094559
Regions
	Domain	149 – 235	87	PDZ 1
	Domain	244 – 330	87	PDZ 2
	Domain	404 – 484	81	PDZ 3
	Domain	519 – 589	71	SH3
	Domain	659 – 834	176	Guanylate kinase-like
Amino acid modifications
	Modified residue	705	1	Phosphotyrosine By similarity
	Modified residue	840	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	627 – 640	14	Missing in isoform Short.		VSP_003151

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 34DA9C46C7BB96DB	FASTA	849	93,539
	10 20 30 40 50 60 MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG YGGYSSQTLP 70 80 90 100 110 120 SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG SGPSWWPECT CTNRDWYEQA 130 140 150 160 170 180 SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI VLERGNSGLG FSIAGGIDNP HVPDDPGIFI 190 200 210 220 230 240 TKIIPGGAAA MDGRLGVNDC VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE 250 260 270 280 290 300 TIMEVNLLKG PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN 310 320 330 340 350 360 NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA LADNHISHNS 370 380 390 400 410 420 SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR EPRKIILHKG STGLGFNIVG 430 440 450 460 470 480 GEDGEGIFVS FILAGGPADL SGELRRGDRI LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV 490 500 510 520 530 540 AQYRPEEYSR FESKIHDLRE QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS 550 560 570 580 590 600 QGLSFSYGDI LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA 610 620 630 640 650 660 RTGMIESNRD FPGLSDDYYG AKNLKGVTSN TSDSESSSKG QEDAILSYEP VTRQEIHYAR 670 680 690 700 710 720 PVIILGPMKD RVNDDLISEF PHKFGSCVPH TTRPRRDNEV DGQDYHFVVS REQMEKDIQD 730 740 750 760 770 780 NKFIEAGQFN DNLYGTSIQS VRAVAERGKH CILDVSGNAI KRLQQAQLYP IAIFIKPKSI 790 800 810 820 830 840 EALMEMNRRQ TYEQANKIFD KAMKLEQEFG EYFTAIVQGD SLEEIYNKIK QIIEDQSGHY IWVPSPEKL
	Isoform Short. Checksum: 95F2227D2F6A7F81	FASTA	835	92,172
	10 20 30 40 50 60 MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG YGGYSSQTLP 70 80 90 100 110 120 SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG SGPSWWPECT CTNRDWYEQA 130 140 150 160 170 180 SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI VLERGNSGLG FSIAGGIDNP HVPDDPGIFI 190 200 210 220 230 240 TKIIPGGAAA MDGRLGVNDC VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE 250 260 270 280 290 300 TIMEVNLLKG PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN 310 320 330 340 350 360 NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA LADNHISHNS 370 380 390 400 410 420 SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR EPRKIILHKG STGLGFNIVG 430 440 450 460 470 480 GEDGEGIFVS FILAGGPADL SGELRRGDRI LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV 490 500 510 520 530 540 AQYRPEEYSR FESKIHDLRE QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS 550 560 570 580 590 600 QGLSFSYGDI LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA 610 620 630 640 650 660 RTGMIESNRD FPGLSDDYYG AKNLKGQEDA ILSYEPVTRQ EIHYARPVII LGPMKDRVND 670 680 690 700 710 720 DLISEFPHKF GSCVPHTTRP RRDNEVDGQD YHFVVSREQM EKDIQDNKFI EAGQFNDNLY 730 740 750 760 770 780 GTSIQSVRAV AERGKHCILD VSGNAIKRLQ QAQLYPIAIF IKPKSIEALM EMNRRQTYEQ 790 800 810 820 830 ANKIFDKAMK LEQEFGEYFT AIVQGDSLEE IYNKIKQIIE DQSGHYIWVP SPEKL

References

[1]	"SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo." Mueller B.M., Kistner U., Kindler S., Chung W.J., Kuhlendahl S., Fenster S.D., Lau L.-F., Veh R.W., Huganir R.L., Gundelfinger E.D., Garner C.C. Neuron 17:255-265(1996) [PubMed: 8780649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INTERACTION WITH GRIN2B. Tissue: Brain.
[2]	"SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density." Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. J. Biol. Chem. 272:11943-11951(1997) [PubMed: 9115257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), INTERACTION WITH DLGAP1; DLGAP2; DLGAP3 AND DLGAP4. Tissue: Brain.
[3]	"SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family." Kim J.H., Liao D., Lau L.-F., Huganir R.L. Neuron 20:683-691(1998) [PubMed: 9581761] [Abstract] Cited for: INTERACTION WITH SYNGAP1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U50147 mRNA. Translation: AAA93031.1. U53367 Genomic DNA. Translation: AAB48561.1.
IPI	IPI00210225. IPI00230936.
RefSeq	NP_113827.1.
UniGene	Rn.10238
3D structure databases
HSSP	HSSP built from PDB template 1PDR based on UniProtKB Q12959.
SMR	Q62936. Positions 144-240, 241-332, 393-493.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62936. 8 interactions.
PTM databases
PhosphoSite	Q62936.
Proteomic databases
PRIDE	Q62936.
Genome annotation databases
Ensembl	ENSRNOG00000002767. Rattus norvegicus. [Contig view]
GeneID	58948.
KEGG	rno:58948.
Organism-specific databases
RGD	68423. Dlgh3.
Phylogenomic databases
HOVERGEN	Q62936.
OMA	Q62936. DPYGPSG.
Gene expression databases
ArrayExpress	Q62936.
GermOnline	ENSRNOG00000002767. Rattus norvegicus.
Family and domain databases
InterPro	IPR019586. Gua_kin_assoc_C. IPR008144. Guanylate_kin. IPR008145. Guanylt/Ca. IPR016313. M-assoc_guanylate_kinase. IPR019590. MAGUK_PEST_N. IPR001478. PDZ/DHR/GLGF. IPR019583. PDZ_assoc. IPR001452. SH3_domain. [Graphical view]
Pfam	PF10603. Gua_kin_assoc_C. 1 hit. PF00625. Guanylate_kin. 1 hit. PF10608. MAGUK_N_PEST. 1 hit. PF00595. PDZ. 3 hits. PF10600. PDZ_assoc. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF001741. MAGUK_DLGH. 1 hit.
ProDom	PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00072. GuKc. 1 hit. SM00228. PDZ. 3 hits. SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. PS50106. PDZ. 3 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611562.

Entry information

Entry name

DLG3_RAT

Accession

Primary (citable) accession number: Q62936
Secondary accession number(s): P70547

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)