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Protein

Disks large homolog 3

Gene

Dlg3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.By similarity

GO - Molecular functioni

  1. guanylate kinase activity Source: GO_Central
  2. ionotropic glutamate receptor binding Source: RGD
  3. PDZ domain binding Source: RGD
  4. phosphatase binding Source: UniProtKB
  5. protein C-terminus binding Source: UniProtKB
  6. protein domain specific binding Source: RGD
  7. protein phosphatase binding Source: BHF-UCL

GO - Biological processi

  1. establishment of planar polarity Source: Ensembl
  2. establishment or maintenance of epithelial cell apical/basal polarity Source: GO_Central
  3. negative regulation of phosphatase activity Source: UniProtKB
  4. nervous system development Source: GO_Central
  5. nucleotide phosphorylation Source: GOC
  6. receptor clustering Source: GO_Central
  7. receptor localization to synapse Source: GO_Central
  8. synaptic transmission Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_343121. NrCAM interactions.
REACT_347698. Activation of Ca-permeable Kainate Receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large homolog 3
Alternative name(s):
PSD-95/SAP90-related protein 1
Synapse-associated protein 102
Short name:
SAP-102
Short name:
SAP102
Gene namesi
Name:Dlg3
Synonyms:Dlgh3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi68423. Dlg3.

Subcellular locationi

GO - Cellular componenti

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: Ensembl
  2. basolateral plasma membrane Source: GO_Central
  3. cytoplasm Source: Ensembl
  4. dendritic shaft Source: UniProtKB
  5. extracellular space Source: Ensembl
  6. growth cone Source: UniProtKB
  7. neuronal cell body Source: UniProtKB
  8. postsynaptic density Source: UniProtKB
  9. postsynaptic membrane Source: GO_Central
  10. synapse Source: UniProtKB
  11. tight junction Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 849849Disks large homolog 3PRO_0000094559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei705 – 7051PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62936.
PRIDEiQ62936.

PTM databases

PhosphoSiteiQ62936.

Expressioni

Gene expression databases

GenevestigatoriQ62936.

Interactioni

Subunit structurei

Interacts through its PDZ domains with NETO1 and APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3 (By similarity). Interacts through its PDZ domains with GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN2. Interacts with LRFN1 and LRFN4. Interacts with FLTP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ATP2B4P23634-62EBI-349596,EBI-1174437From a different organism.
CRIPTQ9P0215EBI-349596,EBI-946968From a different organism.
DgkiQ810C52EBI-349596,EBI-8523614
DgkzO085604EBI-349596,EBI-8570505
GRIN1Q05586-23EBI-349596,EBI-8286218From a different organism.
GRIN2AQ128795EBI-349596,EBI-7249937From a different organism.
GRIN2BQ132244EBI-349596,EBI-2256942From a different organism.
Grin2bQ009606EBI-349596,EBI-396905
GRIN2CQ149576EBI-349596,EBI-8285963From a different organism.
GRIN2DO153992EBI-349596,EBI-1754030From a different organism.
KCNA1Q094702EBI-349596,EBI-8286599From a different organism.
KCNA4P224594EBI-349596,EBI-631235From a different organism.
Lrfn2Q460M52EBI-349596,EBI-877185

Protein-protein interaction databases

BioGridi248683. 5 interactions.
IntActiQ62936. 27 interactions.
MINTiMINT-125016.

Structurei

Secondary structure

1
849
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi403 – 4086Combined sources
Beta strandi413 – 4208Combined sources
Beta strandi422 – 4254Combined sources
Beta strandi427 – 4326Combined sources
Helixi437 – 4415Combined sources
Beta strandi448 – 4536Combined sources
Helixi463 – 47210Combined sources
Beta strandi476 – 4838Combined sources
Helixi485 – 4884Combined sources
Turni489 – 4913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JXTX-ray1.50A/B393-493[»]
ProteinModelPortaliQ62936.
SMRiQ62936. Positions 144-332, 400-493, 522-849.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62936.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 23587PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini244 – 33087PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini404 – 48481PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini519 – 58971SH3PROSITE-ProRule annotationAdd
BLAST
Domaini659 – 834176Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiCOG0194.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000232102.
HOVERGENiHBG107814.
InParanoidiQ62936.
KOiK12075.
OMAiPRRENEM.
OrthoDBiEOG79GT6P.
PhylomeDBiQ62936.
TreeFamiTF323171.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR016313. DLG1.
IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR019590. MAGUK_PEST_N.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23119. PTHR23119. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001741. MAGUK_DLGH. 1 hit.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q62936-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PSGGNGASSG
60 70 80 90 100
YGGYSSQTLP SQAGATPTPR TKAKLIPTGR DVGPVPPKPV PGKNTPKLNG
110 120 130 140 150
SGPSWWPECT CTNRDWYEQA SPAPLLVNPE ALEPSLSVNG SDGMFKYEEI
160 170 180 190 200
VLERGNSGLG FSIAGGIDNP HVPDDPGIFI TKIIPGGAAA MDGRLGVNDC
210 220 230 240 250
VLRVNEVDVS EVVHSRAVEA LKEAGPVVRL VVRRRQPPPE TIMEVNLLKG
260 270 280 290 300
PKGLGFSIAG GIGNQHIPGD NSIYITKIIE GGAAQKDGRL QIGDRLLAVN
310 320 330 340 350
NTNLQDVRHE EAVASLKNTS DMVYLKVAKP GSLHLNDMYA PPDYASTFTA
360 370 380 390 400
LADNHISHNS SLGYLGAVES KVTYPAPPQV PPTRYSPIPR HMLAEEDFTR
410 420 430 440 450
EPRKIILHKG STGLGFNIVG GEDGEGIFVS FILAGGPADL SGELRRGDRI
460 470 480 490 500
LSVNGVNLRN ATHEQAAAAL KRAGQSVTIV AQYRPEEYSR FESKIHDLRE
510 520 530 540 550
QMMNSSMSSG SGSLRTSEKR SLYVRALFDY DRTRDSCLPS QGLSFSYGDI
560 570 580 590 600
LHVINASDDE WWQARLVTPH GESEQIGVIP SKKRVEKKER ARLKTVKFHA
610 620 630 640 650
RTGMIESNRD FPGLSDDYYG AKNLKGVTSN TSDSESSSKG QEDAILSYEP
660 670 680 690 700
VTRQEIHYAR PVIILGPMKD RVNDDLISEF PHKFGSCVPH TTRPRRDNEV
710 720 730 740 750
DGQDYHFVVS REQMEKDIQD NKFIEAGQFN DNLYGTSIQS VRAVAERGKH
760 770 780 790 800
CILDVSGNAI KRLQQAQLYP IAIFIKPKSI EALMEMNRRQ TYEQANKIFD
810 820 830 840
KAMKLEQEFG EYFTAIVQGD SLEEIYNKIK QIIEDQSGHY IWVPSPEKL
Length:849
Mass (Da):93,539
Last modified:November 1, 1997 - v1
Checksum:i34DA9C46C7BB96DB
GO
Isoform Short (identifier: Q62936-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     627-640: Missing.

Show »
Length:835
Mass (Da):92,172
Checksum:i95F2227D2F6A7F81
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei627 – 64014Missing in isoform Short. CuratedVSP_003151Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50147 mRNA. Translation: AAA93031.1.
U53367 Genomic DNA. Translation: AAB48561.1.
RefSeqiNP_113827.1. NM_031639.1. [Q62936-1]
XP_006257144.1. XM_006257082.2. [Q62936-2]
UniGeneiRn.10238.

Genome annotation databases

EnsembliENSRNOT00000003741; ENSRNOP00000003741; ENSRNOG00000002767. [Q62936-1]
ENSRNOT00000045082; ENSRNOP00000047310; ENSRNOG00000002767. [Q62936-2]
GeneIDi58948.
KEGGirno:58948.
UCSCiRGD:68423. rat. [Q62936-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50147 mRNA. Translation: AAA93031.1.
U53367 Genomic DNA. Translation: AAB48561.1.
RefSeqiNP_113827.1. NM_031639.1. [Q62936-1]
XP_006257144.1. XM_006257082.2. [Q62936-2]
UniGeneiRn.10238.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JXTX-ray1.50A/B393-493[»]
ProteinModelPortaliQ62936.
SMRiQ62936. Positions 144-332, 400-493, 522-849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248683. 5 interactions.
IntActiQ62936. 27 interactions.
MINTiMINT-125016.

PTM databases

PhosphoSiteiQ62936.

Proteomic databases

PaxDbiQ62936.
PRIDEiQ62936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003741; ENSRNOP00000003741; ENSRNOG00000002767. [Q62936-1]
ENSRNOT00000045082; ENSRNOP00000047310; ENSRNOG00000002767. [Q62936-2]
GeneIDi58948.
KEGGirno:58948.
UCSCiRGD:68423. rat. [Q62936-1]

Organism-specific databases

CTDi1741.
RGDi68423. Dlg3.

Phylogenomic databases

eggNOGiCOG0194.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000232102.
HOVERGENiHBG107814.
InParanoidiQ62936.
KOiK12075.
OMAiPRRENEM.
OrthoDBiEOG79GT6P.
PhylomeDBiQ62936.
TreeFamiTF323171.

Enzyme and pathway databases

ReactomeiREACT_343121. NrCAM interactions.
REACT_347698. Activation of Ca-permeable Kainate Receptor.

Miscellaneous databases

EvolutionaryTraceiQ62936.
NextBioi611562.
PROiQ62936.

Gene expression databases

GenevestigatoriQ62936.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR016313. DLG1.
IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR019590. MAGUK_PEST_N.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23119. PTHR23119. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001741. MAGUK_DLGH. 1 hit.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo."
    Mueller B.M., Kistner U., Kindler S., Chung W.J., Kuhlendahl S., Fenster S.D., Lau L.-F., Veh R.W., Huganir R.L., Gundelfinger E.D., Garner C.C.
    Neuron 17:255-265(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), INTERACTION WITH GRIN2B.
    Tissue: Brain.
  2. "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
    Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
    J. Biol. Chem. 272:11943-11951(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), INTERACTION WITH DLGAP1; DLGAP2; DLGAP3 AND DLGAP4.
    Tissue: Brain.
  3. "SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family."
    Kim J.H., Liao D., Lau L.-F., Huganir R.L.
    Neuron 20:683-691(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYNGAP1.
  4. "A novel family of adhesion-like molecules that interacts with the NMDA receptor."
    Wang C.Y., Chang K., Petralia R.S., Wang Y.X., Seabold G.K., Wenthold R.J.
    J. Neurosci. 26:2174-2183(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LRFN2.
  5. "Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis."
    Lee H.W., Choi J., Shin H., Kim K., Yang J., Na M., Choi S.Y., Kang G.B., Eom S.H., Kim H., Kim E.
    J. Neurosci. 28:14546-14556(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FRMPD4.

Entry informationi

Entry nameiDLG3_RAT
AccessioniPrimary (citable) accession number: Q62936
Secondary accession number(s): P70547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.