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Protein

Golgi SNAP receptor complex member 1

Gene

Gosr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi SNAP receptor complex member 1
Alternative name(s):
28 kDa Golgi SNARE protein
28 kDa cis-Golgi SNARE p28
Short name:
GOS-28
Short name:
GOS28
Gene namesi
Name:Gosr1
Synonyms:Gs28
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi71093. Gosr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 229228CytoplasmicSequence analysisAdd
BLAST
Transmembranei230 – 25021Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

  • cis-Golgi network Source: RGD
  • cytosol Source: GOC
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 250249Golgi SNAP receptor complex member 1PRO_0000212545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ62931.
PRIDEiQ62931.

PTM databases

iPTMnetiQ62931.
PhosphoSiteiQ62931.

Expressioni

Gene expression databases

GenevisibleiQ62931. RN.

Interactioni

Subunit structurei

Component of several multiprotein Golgi SNARE complexes. Identified in a SNARE complex with BET1, STX5 and YKT6, in a SNARE complex with BET1L, STX5 and YKT6, in a SNARE complex with STX5, GOSR2, SEC22B and BET1, and in complex with STX5 and COG3. Interacts with GABARAPL2. Interacts with the 34 kDa STX5 isoform.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx5Q0885115EBI-7837133,EBI-2028244
Uso1P4154210EBI-7837133,EBI-4423297

Protein-protein interaction databases

IntActiQ62931. 7 interactions.
MINTiMINT-4569995.
STRINGi10116.ENSRNOP00000005283.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili9 – 3022Sequence analysisAdd
BLAST
Coiled coili68 – 9528Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the GOSR1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3208. Eukaryota.
ENOG410YI1X. LUCA.
GeneTreeiENSGT00390000008688.
HOGENOMiHOG000207760.
HOVERGENiHBG051764.
InParanoidiQ62931.
KOiK08495.
OMAiINDKMAE.
OrthoDBiEOG769ZM1.
PhylomeDBiQ62931.
TreeFamiTF105782.

Family and domain databases

InterProiIPR023601. Golgi_SNAP_su1.
[Graphical view]
PANTHERiPTHR21094. PTHR21094. 1 hit.
PIRSFiPIRSF027109. Golgi_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGTSNYWE DLRKQARQLE NELDLKLVSF SKLCTSYSHS SARDGGRDRY
60 70 80 90 100
SSDTTPLLNG SSQDRMFETM AIEIEQLLAR LTGVNDKMAE YTHSAGVPSL
110 120 130 140 150
NAALMHTLQR HRDILQDYTH EFHKTKANFM AIRERENLMG SVRKDIESYK
160 170 180 190 200
SGSGVNNRRT ELFLKEHDHL RNSDRLIEET ISIAMATKEN MTSQRGMLKS
210 220 230 240 250
IHSKMNTLAN RFPAVNSLIQ RINLRKRRDS LILGGVIGIC TILLLLYAFH
Length:250
Mass (Da):28,534
Last modified:November 1, 1996 - v1
Checksum:i0F85EDCE7ADA32E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49099 mRNA. Translation: AAC52597.1.
BC126068 mRNA. Translation: AAI26069.1.
RefSeqiNP_446036.1. NM_053584.2.
UniGeneiRn.8228.

Genome annotation databases

EnsembliENSRNOT00000077063; ENSRNOP00000068434; ENSRNOG00000003971.
GeneIDi94189.
KEGGirno:94189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49099 mRNA. Translation: AAC52597.1.
BC126068 mRNA. Translation: AAI26069.1.
RefSeqiNP_446036.1. NM_053584.2.
UniGeneiRn.8228.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62931. 7 interactions.
MINTiMINT-4569995.
STRINGi10116.ENSRNOP00000005283.

PTM databases

iPTMnetiQ62931.
PhosphoSiteiQ62931.

Proteomic databases

PaxDbiQ62931.
PRIDEiQ62931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000077063; ENSRNOP00000068434; ENSRNOG00000003971.
GeneIDi94189.
KEGGirno:94189.

Organism-specific databases

CTDi9527.
RGDi71093. Gosr1.

Phylogenomic databases

eggNOGiKOG3208. Eukaryota.
ENOG410YI1X. LUCA.
GeneTreeiENSGT00390000008688.
HOGENOMiHOG000207760.
HOVERGENiHBG051764.
InParanoidiQ62931.
KOiK08495.
OMAiINDKMAE.
OrthoDBiEOG769ZM1.
PhylomeDBiQ62931.
TreeFamiTF105782.

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811438. Intra-Golgi traffic.

Miscellaneous databases

PROiQ62931.

Gene expression databases

GenevisibleiQ62931. RN.

Family and domain databases

InterProiIPR023601. Golgi_SNAP_su1.
[Graphical view]
PANTHERiPTHR21094. PTHR21094. 1 hit.
PIRSFiPIRSF027109. Golgi_SNARE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport."
    Subramaniam V.N., Peter F., Philip R., Wong S.H., Hong W.
    Science 272:1161-1163(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-25; 50-53; 76-86; 113-121; 127-133; 145-149; 160-165; 176-187 AND 212-225, FUNCTION.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. "Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells."
    Hay J.C., Chao D.S., Kuo C.S., Scheller R.H.
    Cell 89:149-158(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-26 AND 66-80, SUBUNIT, INTERACTION WITH STX5.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The mammalian protein (rbet1) homologous to yeast Bet1p is primarily associated with the pre-Golgi intermediate compartment and is involved in vesicular transport from the endoplasmic reticulum to the Golgi apparatus."
    Zhang T., Wong S.H., Tang B.L., Xu Y., Peter F., Subramaniam V.N., Hong W.
    J. Cell Biol. 139:1157-1168(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BET1 AND STX5, SUBCELLULAR LOCATION.
  5. "Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs."
    Hay J.C., Klumperman J., Oorschot V., Steegmaier M., Kuo C.S., Scheller R.H.
    J. Cell Biol. 141:1489-1502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STX5, SUBCELLULAR LOCATION.
  6. "Separation of the intracellular secretory compartment of rat liver and isolated rat hepatocytes in a single step using self-generating gradients of iodixanol."
    Plonne D., Cartwright I., Linss W., Dargel R., Graham J.M., Higgins J.A.
    Anal. Biochem. 276:88-96(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28."
    Sagiv Y., Legesse-Miller A., Porat A., Elazar Z.
    EMBO J. 19:1494-1504(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GABARAPL2, SUBCELLULAR LOCATION.
  8. "Ykt6 forms a SNARE complex with syntaxin 5, GS28, and Bet1 and participates in a late stage in endoplasmic reticulum-Golgi transport."
    Zhang T., Hong W.
    J. Biol. Chem. 276:27480-27487(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YKT6; BET1 AND STX5, SUBCELLULAR LOCATION.
  9. "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1."
    Lanoix J., Ouwendijk J., Stark A., Szafer E., Cassel D., Dejgaard K., Weiss M., Nilsson T.
    J. Cell Biol. 155:1199-1212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115."
    Shorter J., Beard M.B., Seemann J., Dirac-Svejstrup A.B., Warren G.
    J. Cell Biol. 157:45-62(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  11. "GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus."
    Xu Y., Martin S., James D.E., Hong W.
    Mol. Biol. Cell 13:3493-3507(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BET1L; STX5 AND YTK6, SUBCELLULAR LOCATION.
  12. "Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack."
    Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M., Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.
    Mol. Biol. Cell 15:1506-1518(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  13. "Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells."
    Zolov S.N., Lupashin V.V.
    J. Cell Biol. 168:747-759(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BET1L AND COG3.
  14. "Heterotypic tubular connections at the endoplasmic reticulum-Golgi complex interface."
    Vivero-Salmeron G., Ballesta J., Martinez-Menarguez J.A.
    Histochem. Cell Biol. 130:709-717(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  15. "The identification of the SNARE complex required for the fusion of VLDL-transport vesicle with hepatic cis-Golgi."
    Siddiqi S., Mani A.M., Siddiqi S.A.
    Biochem. J. 429:391-401(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH SEC22B, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGOSR1_RAT
AccessioniPrimary (citable) accession number: Q62931
Secondary accession number(s): A0JN01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.