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Protein

Complement component C9

Gene

C9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

GO - Biological processi

  • blood coagulation Source: RGD
  • complement activation, alternative pathway Source: UniProtKB-KW
  • complement activation, classical pathway Source: UniProtKB-KW
  • cytolysis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Complement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C9
Gene namesi
Name:C9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620319. C9.

Subcellular locationi

  • Secreted
  • Cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei316 – 332Beta strandedSequence analysisAdd BLAST17
Transmembranei337 – 356Beta strandedSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002360721 – 554Complement component C9Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 76By similarity
Disulfide bondi52 ↔ 55By similarity
Disulfide bondi86 ↔ 92By similarity
Disulfide bondi99 ↔ 110By similarity
Disulfide bondi105 ↔ 123By similarity
Disulfide bondi117 ↔ 132By similarity
Disulfide bondi140 ↔ 179By similarity
Glycosylationi261N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi382 ↔ 413By similarity
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi519 ↔ 535By similarity
Disulfide bondi522 ↔ 537By similarity
Disulfide bondi539 ↔ 548By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei267 – 268Cleavage; by thrombin2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ62930.
PRIDEiQ62930.

PTM databases

iPTMnetiQ62930.
PhosphoSitePlusiQ62930.

Miscellaneous databases

PMAP-CutDBQ62930.

Interactioni

Subunit structurei

Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250734. 1 interactor.
MINTiMINT-4997331.
STRINGi10116.ENSRNOP00000018545.

Structurei

3D structure databases

ProteinModelPortaliQ62930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 93TSP type-1PROSITE-ProRule annotationAdd BLAST54
Domaini97 – 134LDL-receptor class APROSITE-ProRule annotationAdd BLAST38
Domaini136 – 518MACPFPROSITE-ProRule annotationAdd BLAST383
Domaini519 – 549EGF-likeAdd BLAST31

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated
Contains 1 EGF-like domain.Curated
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 MACPF domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG410IH3C. Eukaryota.
ENOG410XT9A. LUCA.
HOVERGENiHBG106792.
InParanoidiQ62930.
KOiK04000.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
SSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGVTITLA IAIFALEINA QAPEPTPREE PSADALLPID CRMSTWSQWS
60 70 80 90 100
QCDPCLKQRF RSRSIEVFGQ FQGKSCADAL GDRQHCEPTQ ECEEVQENCG
110 120 130 140 150
NDFQCETGRC IKRKLLCNGD NDCGDFSDES DCESDPRLPC RDRVVEESEL
160 170 180 190 200
GRTAGYGINI LGMDPLGTPF DNEFYNGLCD RVRDGNTLTY YRKPWNVAFL
210 220 230 240 250
AYETKADKNF RTENYEEQFE MFKTIVRDRT TSFNANLALK FTITEAPIKK
260 270 280 290 300
VGVDEVSPEK NSSKPKDSSV DFQFSYFKKE NFQRLSSYLS QTKKMFLHVK
310 320 330 340 350
GMIQLGRFVM RNRGVMLTTT FLDDVKALPV SYEKGEYFGF LETYGTHYSS
360 370 380 390 400
SGSLGGLYEL IYVLDKASMK EKGVELSDVK RCLGFNLDVS LYTPLQTALE
410 420 430 440 450
GPSLTANVNH SDCLKTGDGK VVNISRDHII DDVISFIRGG TRKQAVLLKE
460 470 480 490 500
KLLRGAKTID VNDFINWASS LDDAPALISQ KLSPIYNLIP LTMKDAYAKK
510 520 530 540 550
QNMEKAIEDY VNEFSARKCY PCQNGGTAIL LDGQCMCSCT IKFKGIACEI

SKQR
Length:554
Mass (Da):62,281
Last modified:November 1, 1996 - v1
Checksum:i9C885F76A1275649
GO

Sequence cautioni

The sequence AAA96528 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65I → M in AAA96528 (Ref. 2) Curated1
Sequence conflicti300K → R in AAA96528 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49071 mRNA. Translation: AAB38023.1.
U52948 mRNA. Translation: AAA96528.1. Different initiation.
RefSeqiNP_476487.1. NM_057146.1.
UniGeneiRn.10252.

Genome annotation databases

GeneIDi117512.
KEGGirno:117512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49071 mRNA. Translation: AAB38023.1.
U52948 mRNA. Translation: AAA96528.1. Different initiation.
RefSeqiNP_476487.1. NM_057146.1.
UniGeneiRn.10252.

3D structure databases

ProteinModelPortaliQ62930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250734. 1 interactor.
MINTiMINT-4997331.
STRINGi10116.ENSRNOP00000018545.

PTM databases

iPTMnetiQ62930.
PhosphoSitePlusiQ62930.

Proteomic databases

PaxDbiQ62930.
PRIDEiQ62930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117512.
KEGGirno:117512.

Organism-specific databases

CTDi735.
RGDi620319. C9.

Phylogenomic databases

eggNOGiENOG410IH3C. Eukaryota.
ENOG410XT9A. LUCA.
HOVERGENiHBG106792.
InParanoidiQ62930.
KOiK04000.

Miscellaneous databases

PMAP-CutDBQ62930.
PROiQ62930.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
SSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO9_RAT
AccessioniPrimary (citable) accession number: Q62930
Secondary accession number(s): Q62957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.