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Protein

cGMP-gated cation channel alpha-1

Gene

Cnga1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei537cGMPSequence analysis1
Binding sitei552cGMPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi478 – 600cGMPSequence analysisAdd BLAST123

GO - Molecular functioni

  • cGMP binding Source: RGD
  • intracellular cAMP activated cation channel activity Source: GO_Central
  • intracellular cGMP activated cation channel activity Source: RGD
  • intracellular cyclic nucleotide activated cation channel activity Source: RGD
  • voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

  • membrane depolarization Source: RGD
  • regulation of cytosolic calcium ion concentration Source: RGD
  • response to stimulus Source: UniProtKB-KW
  • spermatogenesis Source: RGD
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-gated cation channel alpha-1
Alternative name(s):
Cyclic nucleotide-gated cation channel 1
Cyclic nucleotide-gated channel alpha-1
Short name:
CNG channel alpha-1
Short name:
CNG-1
Short name:
CNG1
Cyclic nucleotide-gated channel, photoreceptor
Rod photoreceptor cGMP-gated channel subunit alpha
Gene namesi
Name:Cnga1
Synonyms:Cncg, Cncg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621815. Cnga1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 155CytoplasmicSequence analysisAdd BLAST155
Transmembranei156 – 176Helical; Name=H1Sequence analysisAdd BLAST21
Topological domaini177 – 189ExtracellularSequence analysisAdd BLAST13
Transmembranei190 – 208Helical; Name=H2Sequence analysisAdd BLAST19
Topological domaini209 – 232CytoplasmicSequence analysisAdd BLAST24
Transmembranei233 – 252Helical; Name=H3Sequence analysisAdd BLAST20
Topological domaini253 – 290ExtracellularSequence analysisAdd BLAST38
Transmembranei291 – 313Helical; Name=H4Sequence analysisAdd BLAST23
Topological domaini314 – 365CytoplasmicSequence analysisAdd BLAST52
Transmembranei366 – 385Helical; Name=H5Sequence analysisAdd BLAST20
Topological domaini386 – 469ExtracellularSequence analysisAdd BLAST84
Transmembranei470 – 490Helical; Name=H6Sequence analysisAdd BLAST21
Topological domaini491 – 683CytoplasmicSequence analysisAdd BLAST193

GO - Cellular componenti

  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • intracellular cyclic nucleotide activated cation channel complex Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi394.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193101 – 683cGMP-gated cation channel alpha-1Add BLAST683

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi416N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ62927.
PRIDEiQ62927.

PTM databases

PhosphoSitePlusiQ62927.

Expressioni

Tissue specificityi

Rod cells in the retina.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.By similarity

Protein-protein interaction databases

IntActiQ62927. 1 interactor.
MINTiMINT-8057701.
STRINGi10116.ENSRNOP00000006469.

Structurei

3D structure databases

ProteinModelPortaliQ62927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili614 – 657By similarityAdd BLAST44

Domaini

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ62927.
KOiK04948.
PhylomeDBiQ62927.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTNIINTWH SFVNIPNVVV PAIEKEIRRM ENGACSSFSD NDNGSLSEES
60 70 80 90 100
ENEDSLFRSN SYRRRGPSQR EHYLPGTMAL FNVNNSSNKD QDPKEKKKKK
110 120 130 140 150
KEKKSKADDK KESKKDPEKK KKKEKEKEKK KEEKPKEKKE EEKKEVVVID
160 170 180 190 200
PSGNMYYNWL FCITLPVMYN WTMIIARACF DELQSDYLEY WLIFDYVSDV
210 220 230 240 250
VYLADMFVRT RTGYLEQGLL VKDELKLIEK YKANLQFKLD VLSVIPTDLL
260 270 280 290 300
YFKFGWNYPE IRLNRLLRIS RMFEFFQRTE TRTNYPNIFR ISNLVMYIVI
310 320 330 340 350
IIHWNACVYY SISKAIGFGN DTWVYPDVND PEFGRLARKY VYSLYWSTLT
360 370 380 390 400
LTTIGETPPP VLDSEYVFVV VDFLIGVLIF ATIVGNIGSM ISNMNAARAE
410 420 430 440 450
FQSRVDAIKQ YMNFRNVSKD MEKRVIKWFD YLWTNKKTVD EREVLRYLPD
460 470 480 490 500
KLRAEIAINV HLDTLKKVRI FADCEAGLLV ELVLKLQPQV YSPGDYICKK
510 520 530 540 550
GDIGREMYII KEGKLAVVAD DGITQFVVLS DGSYFGEISI LNIKGSKAGN
560 570 580 590 600
RRTANIKSIG YSDLFCLSKD DLMEALTEYP DAKTMLEEKG RQILMKDGLL
610 620 630 640 650
DINIANLGSD PKDLEEKVTR MEGSVDLLQT RFARILAEYE SMQQKLKQRL
660 670 680
TKVEKFLKPL IETEFSALEE PGGESEPTES LQG
Length:683
Mass (Da):79,228
Last modified:November 1, 1997 - v1
Checksum:i62D49F64F8D917C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 4TN → KV in AAC53139 (PubMed:9142860).Curated2
Sequence conflicti141E → G in AAC53139 (PubMed:9142860).Curated1
Sequence conflicti224E → K in AAC53139 (PubMed:9142860).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48803 Genomic DNA. Translation: AAA92110.1.
U93851 mRNA. Translation: AAC53139.1.
U76220 mRNA. Translation: AAC17594.1.
RefSeqiNP_445949.1. NM_053497.1.
UniGeneiRn.54534.

Genome annotation databases

GeneIDi85259.
KEGGirno:85259.
UCSCiRGD:621815. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48803 Genomic DNA. Translation: AAA92110.1.
U93851 mRNA. Translation: AAC53139.1.
U76220 mRNA. Translation: AAC17594.1.
RefSeqiNP_445949.1. NM_053497.1.
UniGeneiRn.54534.

3D structure databases

ProteinModelPortaliQ62927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62927. 1 interactor.
MINTiMINT-8057701.
STRINGi10116.ENSRNOP00000006469.

Chemistry databases

GuidetoPHARMACOLOGYi394.

PTM databases

PhosphoSitePlusiQ62927.

Proteomic databases

PaxDbiQ62927.
PRIDEiQ62927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85259.
KEGGirno:85259.
UCSCiRGD:621815. rat.

Organism-specific databases

CTDi1259.
RGDi621815. Cnga1.

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ62927.
KOiK04948.
PhylomeDBiQ62927.

Miscellaneous databases

PROiQ62927.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNGA1_RAT
AccessioniPrimary (citable) accession number: Q62927
Secondary accession number(s): O08659
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.