ID   PNOC_RAT                Reviewed;         181 AA.
AC   Q62923; Q64162;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Prepronociceptin;
DE   Contains:
DE     RecName: Full=Nocistatin;
DE   Contains:
DE     RecName: Full=Nociceptin;
DE     AltName: Full=ORL1 receptor agonist;
DE     AltName: Full=Orphanin FQ;
DE     AltName: Full=PPNOC;
DE   Contains:
DE     RecName: Full=Orphanin FQ2;
DE   Flags: Precursor;
GN   Name=Pnoc;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=Sprague-Dawley;
RX   PubMed=8710930; DOI=10.1073/pnas.93.16.8677;
RA   Nothacker H.-P., Reinscheid R.K., Mansour A., Henningsen R.A., Ardati A.,
RA   Monsma F.J. Jr., Watson S.J., Civelli O.;
RT   "Primary structure and tissue distribution of the orphanin FQ precursor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:8677-8682(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=8710928; DOI=10.1073/pnas.93.16.8666;
RA   Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G.,
RA   Meunier J.-C., Parmentier M.;
RT   "Structure, tissue distribution, and chromosomal localization of the
RT   prepronociceptin gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 13-181, PROTEIN SEQUENCE OF 135-151, AND
RP   FUNCTION.
RC   TISSUE=Brain;
RX   PubMed=7566152; DOI=10.1038/377532a0;
RA   Meunier J.-C., Mollereau C., Toll L., Suaudeau C., Moisand C.,
RA   Alvinerie P., Butour J.-L., Guillemot J.-C., Ferrara P., Monsarrat B.,
RA   Mazarguil H., Vassart G., Parmentier M., Costentin J.;
RT   "Isolation and structure of the endogenous agonist of opioid receptor-like
RT   ORL1 receptor.";
RL   Nature 377:532-535(1995).
CC   -!- FUNCTION: [Nociceptin]: Ligand of the opioid receptor-like receptor
CC       OPRL1. It may act as a transmitter in the brain by modulating
CC       nociceptive and locomotor behavior. May be involved in neuronal
CC       differentiation and development. {ECO:0000269|PubMed:7566152}.
CC   -!- FUNCTION: [Nocistatin]: Blocks nociceptin action in pain transmission
CC       by inhibiting nociceptin-induced hyperalgesia and allodynia.
CC       {ECO:0000250|UniProtKB:O62647}.
CC   -!- FUNCTION: [Orphanin FQ2]: Has potent analgesic activity.
CC       {ECO:0000250|UniProtKB:Q64387}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in the spinal cord and
CC       brain, being more abundant in the hypothalamus and striatum. Also found
CC       in small amounts in ovary. {ECO:0000269|PubMed:8710928,
CC       ECO:0000269|PubMed:8710930}.
CC   -!- PTM: Specific enzymatic cleavages at paired basic residues probably
CC       yield other active peptides besides nociceptin.
CC   -!- PTM: The N-terminal domain contains 6 conserved cysteines thought to be
CC       involved in disulfide bonding and/or processing.
CC   -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC       {ECO:0000305}.
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DR   EMBL; U48262; AAC52726.1; -; mRNA.
DR   EMBL; X97375; CAA66043.1; -; mRNA.
DR   EMBL; S79730; AAB35376.1; -; mRNA.
DR   PIR; JC6151; JC6151.
DR   RefSeq; NP_037139.1; NM_013007.1.
DR   RefSeq; XP_006252261.1; XM_006252199.3.
DR   AlphaFoldDB; Q62923; -.
DR   SMR; Q62923; -.
DR   STRING; 10116.ENSRNOP00000019117; -.
DR   PhosphoSitePlus; Q62923; -.
DR   PaxDb; 10116-ENSRNOP00000019117; -.
DR   Ensembl; ENSRNOT00000019117.8; ENSRNOP00000019117.6; ENSRNOG00000014231.8.
DR   Ensembl; ENSRNOT00055010837; ENSRNOP00055008421; ENSRNOG00055006648.
DR   Ensembl; ENSRNOT00060018417; ENSRNOP00060014338; ENSRNOG00060010866.
DR   Ensembl; ENSRNOT00065031257; ENSRNOP00065024897; ENSRNOG00065018612.
DR   GeneID; 25516; -.
DR   KEGG; rno:25516; -.
DR   UCSC; RGD:3362; rat.
DR   AGR; RGD:3362; -.
DR   CTD; 5368; -.
DR   RGD; 3362; Pnoc.
DR   eggNOG; ENOG502S0DD; Eukaryota.
DR   GeneTree; ENSGT00950000183149; -.
DR   HOGENOM; CLU_143892_0_0_1; -.
DR   InParanoid; Q62923; -.
DR   OMA; CREKLHP; -.
DR   OrthoDB; 5312719at2759; -.
DR   PhylomeDB; Q62923; -.
DR   Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR   Reactome; R-RNO-418594; G alpha (i) signalling events.
DR   PRO; PR:Q62923; -.
DR   Proteomes; UP000002494; Chromosome 15.
DR   Bgee; ENSRNOG00000014231; Expressed in cerebellum and 6 other cell types or tissues.
DR   GO; GO:0043679; C:axon terminus; IBA:GO_Central.
DR   GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; ISO:RGD.
DR   GO; GO:0097060; C:synaptic membrane; ISO:RGD.
DR   GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR   GO; GO:0031628; F:opioid receptor binding; IBA:GO_Central.
DR   GO; GO:0048018; F:receptor ligand activity; ISO:RGD.
DR   GO; GO:0007268; P:chemical synaptic transmission; IDA:RGD.
DR   GO; GO:0007565; P:female pregnancy; IEP:RGD.
DR   GO; GO:0007270; P:neuron-neuron synaptic transmission; ISO:RGD.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IDA:RGD.
DR   GO; GO:0007600; P:sensory perception; IBA:GO_Central.
DR   GO; GO:0019233; P:sensory perception of pain; ISO:RGD.
DR   InterPro; IPR002367; Nociceptin.
DR   InterPro; IPR006024; Opioid_neupept.
DR   PANTHER; PTHR11438:SF2; PREPRONOCICEPTIN; 1.
DR   PANTHER; PTHR11438; PROENKEPHALIN; 1.
DR   Pfam; PF01160; Opiods_neuropep; 1.
DR   PRINTS; PR01028; OPIOIDPRCRSR.
DR   PRINTS; PR01031; ORPHNNPRCRSR.
DR   PROSITE; PS01252; OPIOIDS_PRECURSOR; 1.
DR   Genevisible; Q62923; RN.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Neuropeptide; Neurotransmitter; Opioid peptide;
KW   Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..95
FT                   /id="PRO_0000008335"
FT   PEPTIDE         98..132
FT                   /note="Nocistatin"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000008336"
FT   PEPTIDE         135..151
FT                   /note="Nociceptin"
FT                   /evidence="ECO:0000269|PubMed:7566152"
FT                   /id="PRO_0000008337"
FT   PEPTIDE         154..170
FT                   /note="Orphanin FQ2"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000008338"
FT   PROPEP          174..181
FT                   /id="PRO_0000008339"
FT   REPEAT          109..114
FT                   /note="1"
FT   REPEAT          115..120
FT                   /note="2"
FT   REGION          103..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          109..120
FT                   /note="2 X 6 AA tandem repeats of D-A-E-P-X-A"
SQ   SEQUENCE   181 AA;  20479 MW;  B2E9119CF8702C37 CRC64;
     MKILFCDVLL LSLLSSVFSS CPEDCLTCQE RLHPAPGSFN LKLCILQCEE KVFPRPLWTL
     CTKAMASDSE QLSPADPELT SAALYQSKAS EMQHLKRMPR VRSVVQARDA EPEADAEPVA
     DEADEVEQKQ LQKRFGGFTG ARKSARKLAN QKRFSEFMRQ YLVLSMQSSQ RRRTLHQNGN
     V
//