Q62923 (PNOC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prepronociceptin

Cleaved into the following 3 chains:

1. Neuropeptide 1
2. Nociceptin
   Alternative name(s):
   ORL1 receptor agonist
   Orphanin FQ
   PPNOC
3. Neuropeptide 2

Gene names

Name:

Pnoc

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
Subcellular location	Secreted.
Tissue specificity	Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary.
Post-translational modification	Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Neuropeptide Neurotransmitter Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	neuropeptide signaling pathway Inferred from direct assay PubMed 11826101. Source: RGD synaptic transmission Inferred from direct assay PubMed 11826101. Source: RGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	opioid peptide activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 95	76			PRO_0000008335
	Peptide	98 – 132	35	Neuropeptide 1 Probable		PRO_0000008336
	Peptide	135 – 151	17	Nociceptin Ref.3		PRO_0000008337
	Peptide	154 – 170	17	Neuropeptide 2 Probable		PRO_0000008338
	Propeptide	174 – 181	8			PRO_0000008339
Regions
	Repeat	109 – 114	6	1
	Repeat	115 – 120	6	2
	Region	109 – 120	12	2 X 6 AA tandem repeats of D-A-E-P-X-A

Sequences

	Sequence		Length	Mass (Da)
	Q62923 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B2E9119CF8702C37	FASTA	181	20,479
	10 20 30 40 50 60 MKILFCDVLL LSLLSSVFSS CPEDCLTCQE RLHPAPGSFN LKLCILQCEE KVFPRPLWTL 70 80 90 100 110 120 CTKAMASDSE QLSPADPELT SAALYQSKAS EMQHLKRMPR VRSVVQARDA EPEADAEPVA 130 140 150 160 170 180 DEADEVEQKQ LQKRFGGFTG ARKSARKLAN QKRFSEFMRQ YLVLSMQSSQ RRRTLHQNGN V

References

[1]	"Primary structure and tissue distribution of the orphanin FQ precursor." Nothacker H.-P., Reinscheid R.K., Mansour A., Henningsen R.A., Ardati A., Monsma F.J. Jr., Watson S.J., Civelli O. Proc. Natl. Acad. Sci. U.S.A. 93:8677-8682(1996) [PubMed: 8710930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene." Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M. Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed: 8710928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation and structure of the endogenous agonist of opioid receptor-like ORL1 receptor." Meunier J.-C., Mollereau C., Toll L., Suaudeau C., Moisand C., Alvinerie P., Butour J.-L., Guillemot J.-C., Ferrara P., Monsarrat B., Mazarguil H., Vassart G., Parmentier M., Costentin J. Nature 377:532-535(1995) [PubMed: 7566152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-181, PROTEIN SEQUENCE OF 135-151. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48262 mRNA. Translation: AAC52726.1. X97375 mRNA. Translation: CAA66043.1. S79730 mRNA. Translation: AAB35376.1.
IPI	IPI00210192.
PIR	JC6151.
RefSeq	NP_037139.1. NM_013007.1.
UniGene	Rn.87935.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q62923.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25516.
KEGG	rno:25516.
Organism-specific databases
CTD	5368.
RGD	3362. Pnoc.
Phylogenomic databases
eggNOG	NOG40726.
HOGENOM	HOG000082421.
HOVERGEN	HBG008227.
InParanoid	Q62923.
OrthoDB	EOG44BB3B.
Gene expression databases
ArrayExpress	Q62923.
Genevestigator	Q62923.
GermOnline	ENSRNOG00000014231. Rattus norvegicus.
Family and domain databases
InterPro	IPR002367. Nociceptin. IPR006024. Opioid_neupept. [Graphical view]
PANTHER	PTHR11438. Opioid_neupept. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01031. ORPHNNPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606969.

Entry information

Entry name

PNOC_RAT

Accession

Primary (citable) accession number: Q62923
Secondary accession number(s): Q64162

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 16, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program