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Protein

RanBP-type and C3HC4-type zinc finger-containing protein 1

Gene

Rbck1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity). E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri188 – 22033RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri280 – 32546RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri360 – 40950IBR-typeAdd
BLAST
Zinc fingeri435 – 46127RING-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD
  • ligase activity Source: UniProtKB-KW
  • protein kinase C binding Source: RGD
  • ubiquitin binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
RanBP-type and C3HC4-type zinc finger-containing protein 1 (EC:6.3.2.-)
Alternative name(s):
Heme-oxidized IRP2 ubiquitin ligase 1 homolog
Short name:
HOIL-1
Protein kinase C-binding protein beta-15
RBCC protein interacting with PKC
Ubiquitin-conjugating enzyme 7-interacting protein 3
Gene namesi
Name:Rbck1
Synonyms:Pkcbpb15, Rbck, Ubce7ip3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708404. Rbck1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi303 – 3031C → G: Greatly reduces auto-ubiquitination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508RanBP-type and C3HC4-type zinc finger-containing protein 1PRO_0000056297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei328 – 3281PhosphotyrosineBy similarity

Post-translational modificationi

Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome.1 Publication
Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62921.
PRIDEiQ62921.

PTM databases

PhosphoSiteiQ62921.

Expressioni

Tissue specificityi

Widely expressed.

Interactioni

Subunit structurei

Forms homodimers in vitro (By similarity). Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Interacts with beta-I-type (PRKCB1) and zeta-type protein kinase C (PRKCZ) and with UBE2L3. Interacts with IREB2 only in iron-rich conditions. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Interacts with EYA1, TAB2, TAB3, MAP3K7 TRAF6 and RIPK1. Interacts with IRF3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMD4P550363EBI-7266339,EBI-359318From a different organism.
UBCP0CG482EBI-7266339,EBI-3390054From a different organism.

GO - Molecular functioni

  • protein kinase C binding Source: RGD
  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi248810. 4 interactions.
IntActiQ62921. 2 interactions.
MINTiMINT-7261390.
STRINGi10116.ENSRNOP00000047637.

Structurei

3D structure databases

ProteinModelPortaliQ62921.
SMRiQ62921. Positions 192-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 11965Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 268268Interaction with TAB2By similarityAdd
BLAST
Regioni1 – 218218Interaction with IRF3By similarityAdd
BLAST
Regioni69 – 13163Interaction with RNF31By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili231 – 26030Sequence analysisAdd
BLAST

Domaini

The RanBP2-type zinc finger, also called Npl4 zinc finger (NZF), mediates binding to 'Met-1'-linked polyubiquitins.By similarity
The UBL domain mediates association with RNF31 via interaction with its UBA domain.By similarity

Sequence similaritiesi

Contains 1 IBR-type zinc finger.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri188 – 22033RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri280 – 32546RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri360 – 40950IBR-typeAdd
BLAST
Zinc fingeri435 – 46127RING-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
HOVERGENiHBG061515.
InParanoidiQ62921.
KOiK10630.
PhylomeDBiQ62921.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR002867. IBR_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR027370. Znf-RING_LisH.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF01485. IBR. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62921-1) [UniParc]FASTAAdd to basket

Also known as: RBCK1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEKTKKAEE MALSLARAVT GGDEQAAIKY ATWLAEQKVP LRVQVKPEVS
60 70 80 90 100
PTQDIRLCVS VEDAYMHTVT IWLTVRPDMT VASLKDMVFL DYGFPPSLQQ
110 120 130 140 150
WVVGQRLARD QETLHSHGIR RNGDSAYLYL LSARNTSLNP QELQRQRQLR
160 170 180 190 200
MLEDLGFKDL TLQPRGPLEP VLPKPRTHQE TGQPDAAPES PPVGWQCPGC
210 220 230 240 250
TFINKPTRPG CEMCCRARPE AYQIPASYQP DEEERARLAG EEEALRQYEQ
260 270 280 290 300
RKQQQQEGNY LQHVQLEQRS LVLNTEPAEC PVCYSVLAPG EAVVLRECLH
310 320 330 340 350
TFCRECLQGT IRNSQEAEVS CPFIDNTYSC PGKLLEREIR ALLSPEDYQR
360 370 380 390 400
FLDLGVSIAE NRSTLSYHCK TPDCRGWCFF EDDVNEFTCP VCTRVNCLLC
410 420 430 440 450
KAIHERMNCR EYQDDLAHRA RNDVAAQQTT EMLRVMLQQG EAMYCPQCRI
460 470 480 490 500
VVQKKDGCDW IRCTVCHTEI CWVTKGPRWG PGGPGDTSGG CRCRVNGIPC

HPSCQNCH
Length:508
Mass (Da):57,685
Last modified:January 15, 2008 - v3
Checksum:iD2E407B254E9FDA6
GO
Isoform 2 (identifier: Q62921-2) [UniParc]FASTAAdd to basket

Also known as: RBCK2

The sequence of this isoform differs from the canonical sequence as follows:
     251-270: RKQQQQEGNYLQHVQLEQRS → GVPAGYHPKQPGGGGILPLH
     271-508: Missing.

Show »
Length:270
Mass (Da):30,278
Checksum:iD689295BBC4ED744
GO

Sequence cautioni

The sequence AAC72243.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA33957.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei251 – 27020RKQQQ…LEQRS → GVPAGYHPKQPGGGGILPLH in isoform 2. 1 PublicationVSP_005769Add
BLAST
Alternative sequencei271 – 508238Missing in isoform 2. 1 PublicationVSP_005770Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48248 mRNA. Translation: AAC72243.1. Different initiation.
AB007133 Genomic DNA. Translation: BAA33953.1.
AB011369 mRNA. Translation: BAA33957.1. Different initiation.
PIRiJC5983.
RefSeqiNP_068532.2. NM_021764.1. [Q62921-1]
UniGeneiRn.37161.

Genome annotation databases

GeneIDi60383.
KEGGirno:60383.
UCSCiRGD:708404. rat. [Q62921-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48248 mRNA. Translation: AAC72243.1. Different initiation.
AB007133 Genomic DNA. Translation: BAA33953.1.
AB011369 mRNA. Translation: BAA33957.1. Different initiation.
PIRiJC5983.
RefSeqiNP_068532.2. NM_021764.1. [Q62921-1]
UniGeneiRn.37161.

3D structure databases

ProteinModelPortaliQ62921.
SMRiQ62921. Positions 192-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248810. 4 interactions.
IntActiQ62921. 2 interactions.
MINTiMINT-7261390.
STRINGi10116.ENSRNOP00000047637.

PTM databases

PhosphoSiteiQ62921.

Proteomic databases

PaxDbiQ62921.
PRIDEiQ62921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60383.
KEGGirno:60383.
UCSCiRGD:708404. rat. [Q62921-1]

Organism-specific databases

CTDi10616.
RGDi708404. Rbck1.

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
HOVERGENiHBG061515.
InParanoidiQ62921.
KOiK10630.
PhylomeDBiQ62921.

Enzyme and pathway databases

BRENDAi2.3.2.B10. 5301.

Miscellaneous databases

NextBioi612079.
PROiQ62921.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR002867. IBR_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR027370. Znf-RING_LisH.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF01485. IBR. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a novel protein kinase C-interacting protein with structural motifs related to RBCC family proteins."
    Tokunaga C., Kuroda S.I., Tatematsu K., Nakagawa N., Ono Y., Kikkawa U.
    Biochem. Biophys. Res. Commun. 244:353-359(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular cloning and characterization of RBCK2, a splicing variant of a RBCC family protein, RBCK1."
    Tokunaga C., Takematsu K., Kuroda S.I., Nakagawa N., Kikkawa U.
    FEBS Lett. 435:11-15(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cbeta."
    Tatematsu K., Yoshimoto N., Okajima T., Tanizawa K., Kuroda S.
    J. Biol. Chem. 283:11575-11585(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOUBIQUITINATION, PHOSPHORYLATION, RING-TYPE ZINC FINGERS, MUTAGENESIS OF CYS-303.

Entry informationi

Entry nameiHOIL1_RAT
AccessioniPrimary (citable) accession number: Q62921
Secondary accession number(s): Q9QWN4, Q9Z334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 15, 2008
Last modified: May 11, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.