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Protein

PDZ and LIM domain protein 5

Gene

Pdlim5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Isoforms lacking the LIM domains may negatively modulate the scaffolding activity of isoform 1. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses.2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actinin binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • protein N-terminus binding Source: RGD
  • receptor signaling complex scaffold activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell growth involved in cardiac muscle cell development Source: BHF-UCL
  • heart development Source: UniProtKB
  • regulation of dendritic spine morphogenesis Source: UniProtKB
  • regulation of synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PDZ and LIM domain protein 5
Alternative name(s):
Enigma homolog
Enigma-like PDZ and LIM domains protein
Gene namesi
Name:Pdlim5
Synonyms:Enh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621076. Pdlim5.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
  • neuron projection Source: UniProtKB-SubCell
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 591590PDZ and LIM domain protein 5PRO_0000075879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei89 – 891N6-acetyllysine; alternateBy similarity
Modified residuei89 – 891N6-succinyllysine; alternateBy similarity
Modified residuei111 – 1111PhosphoserineBy similarity
Modified residuei134 – 1341PhosphoserineBy similarity
Modified residuei137 – 1371PhosphoserineBy similarity
Modified residuei228 – 2281PhosphoserineCombined sources
Modified residuei260 – 2601PhosphoserineBy similarity
Modified residuei313 – 3131PhosphoserineBy similarity
Modified residuei318 – 3181PhosphoserineCombined sources
Modified residuei346 – 3461N6-acetyllysineBy similarity
Modified residuei355 – 3551PhosphoserineCombined sources
Modified residuei357 – 3571PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62920.
PRIDEiQ62920.

PTM databases

iPTMnetiQ62920.
PhosphoSiteiQ62920.

Expressioni

Tissue specificityi

Detected in brain, in neurons and glial cells (at protein level). Detected in heart and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSRNOG00000016419.
GenevisibleiQ62920. RN.

Interactioni

Subunit structurei

Interacts with various PKC isoforms through the LIM domains. Interacts with actin and alpha-actinin through the PDZ domain. Interacts (via LIM domains) with SIPA1L1.3 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actinin binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • protein N-terminus binding Source: RGD
  • receptor signaling complex scaffold activity Source: UniProtKB

Protein-protein interaction databases

IntActiQ62920. 1 interaction.
STRINGi10116.ENSRNOP00000022387.

Structurei

3D structure databases

ProteinModelPortaliQ62920.
SMRiQ62920. Positions 1-85, 395-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8584PDZPROSITE-ProRule annotationAdd
BLAST
Domaini413 – 47260LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini472 – 53160LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini531 – 59161LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000220936.
HOVERGENiHBG051478.
InParanoidiQ62920.
OMAiHATENST.
OrthoDBiEOG091G0A45.
PhylomeDBiQ62920.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
2.30.42.10. 1 hit.
InterProiIPR031847. DUF4749.
IPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF15936. DUF4749. 1 hit.
PF00412. LIM. 3 hits.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q62920-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNYNVSLVG PAPWGFRLQG GKDFNMPLTI SSLKDGGKAS QAHVRIGDVV
60 70 80 90 100
LSIDGISAQG MTHLEAQNKI KACTGSLNMT LQRASAAAKS EPVAVQKGEP
110 120 130 140 150
KEVVKPVPIT SPAVSKVTST TNMAYNKVPR PFGSVSSPKV TSIPSPSSAF
160 170 180 190 200
TPAHAATSSH ASPPPVAAVT PPPLSASGLH ASANPSAAQC SSPPNTGKPA
210 220 230 240 250
VHVPRQPTVT SVCSESAQEL AEGQRRGSQG DIKQQNGPPR KHIVERNTEF
260 270 280 290 300
YHIPTHSDAS KKRLIEDTED WRPRTGTTQS RSFRILAQIT GTEHLKESEN
310 320 330 340 350
DNAKKANSTP EPSQQSASPL SAAESLESPG SNRPVVAGLR SAAAFKPVGS
360 370 380 390 400
TSVKSPSWQR PNQAAPSTGR ISNSASSSGT GAPMKPAVGP PQPSDQDTLV
410 420 430 440 450
QRAEHIPAGK RTPMCAHCNQ AIRGPFLVAL GKSWHPEEFN CAHCKNTMAY
460 470 480 490 500
IGFVEEKGAL YCELCYEKFF APECGRCQRK ILGEVINALK QTWHVSCFVC
510 520 530 540 550
VACGKPIRNN VFHLEDGEPY CETDYYALFG TICRGCEFPI EAGDMFLEAL
560 570 580 590
GSTWHDTCFV CSVCCESLEG QTFFSKKDKP LCKKHAHSVN F
Length:591
Mass (Da):63,201
Last modified:January 1, 1999 - v2
Checksum:i7B26FB9BE1680299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48247 mRNA. Translation: AAC72251.1.
RefSeqiNP_445778.1. NM_053326.1. [Q62920-1]
UniGeneiRn.17933.

Genome annotation databases

EnsembliENSRNOT00000022387; ENSRNOP00000022387; ENSRNOG00000016419. [Q62920-1]
GeneIDi64353.
KEGGirno:64353.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48247 mRNA. Translation: AAC72251.1.
RefSeqiNP_445778.1. NM_053326.1. [Q62920-1]
UniGeneiRn.17933.

3D structure databases

ProteinModelPortaliQ62920.
SMRiQ62920. Positions 1-85, 395-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62920. 1 interaction.
STRINGi10116.ENSRNOP00000022387.

PTM databases

iPTMnetiQ62920.
PhosphoSiteiQ62920.

Proteomic databases

PaxDbiQ62920.
PRIDEiQ62920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022387; ENSRNOP00000022387; ENSRNOG00000016419. [Q62920-1]
GeneIDi64353.
KEGGirno:64353.

Organism-specific databases

CTDi10611.
RGDi621076. Pdlim5.

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000220936.
HOVERGENiHBG051478.
InParanoidiQ62920.
OMAiHATENST.
OrthoDBiEOG091G0A45.
PhylomeDBiQ62920.

Miscellaneous databases

PROiQ62920.

Gene expression databases

BgeeiENSRNOG00000016419.
GenevisibleiQ62920. RN.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
2.30.42.10. 1 hit.
InterProiIPR031847. DUF4749.
IPR001478. PDZ.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF15936. DUF4749. 1 hit.
PF00412. LIM. 3 hits.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDLI5_RAT
AccessioniPrimary (citable) accession number: Q62920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.