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Protein

Protein kinase C-binding protein NELL2

Gene

Nell2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for neuron survival through the modulation of MAPK pathways (PubMed:12941464). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heparin binding Source: RGD
  3. identical protein binding Source: RGD
  4. protein kinase C binding Source: RGD

GO - Biological processi

  1. development of secondary female sexual characteristics Source: UniProtKB
  2. neuron cellular homeostasis Source: UniProtKB
  3. positive regulation of hormone secretion Source: UniProtKB
  4. protein homotrimerization Source: RGD
  5. regulation of growth Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-binding protein NELL2
Alternative name(s):
NEL-like protein 2
Gene namesi
Name:Nell2
Synonyms:Nel
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620999. Nell2.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. dendrite Source: RGD
  3. extracellular space Source: RGD
  4. membrane Source: RGD
  5. perikaryon Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 816795Protein kinase C-binding protein NELL2PRO_0000007668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi401 ↔ 413PROSITE-ProRule annotation
Disulfide bondi407 ↔ 422PROSITE-ProRule annotation
Disulfide bondi424 ↔ 438PROSITE-ProRule annotation
Disulfide bondi444 ↔ 457PROSITE-ProRule annotation
Disulfide bondi451 ↔ 466PROSITE-ProRule annotation
Disulfide bondi468 ↔ 480PROSITE-ProRule annotation
Disulfide bondi486 ↔ 499PROSITE-ProRule annotation
Disulfide bondi493 ↔ 508PROSITE-ProRule annotation
Disulfide bondi510 ↔ 521PROSITE-ProRule annotation
Glycosylationi517 – 5171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi525 ↔ 535PROSITE-ProRule annotation
Disulfide bondi529 ↔ 541PROSITE-ProRule annotation
Disulfide bondi543 ↔ 552PROSITE-ProRule annotation
Disulfide bondi559 ↔ 572PROSITE-ProRule annotation
Disulfide bondi566 ↔ 581PROSITE-ProRule annotation
Disulfide bondi583 ↔ 600PROSITE-ProRule annotation
Disulfide bondi606 ↔ 619PROSITE-ProRule annotation
Disulfide bondi613 ↔ 628PROSITE-ProRule annotation
Glycosylationi615 – 6151N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi630 ↔ 636PROSITE-ProRule annotation
Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ62918.

Expressioni

Tissue specificityi

Widely expressed in brain (PubMed:18547942). High expression is observed in telencephalic and diencephalic glutamatergic neurons, while no expression is found in GABAergic and GNRH neurons (PubMed:18547942).1 Publication

Gene expression databases

GenevestigatoriQ62918.

Interactioni

Subunit structurei

Homotrimer. Binds to PKC beta-1.

Structurei

3D structure databases

ProteinModelPortaliQ62918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 228174Laminin G-likeAdd
BLAST
Domaini272 – 33160VWFC 1PROSITE-ProRule annotationAdd
BLAST
Domaini397 – 43943EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini440 – 48142EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini482 – 52241EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini523 – 55331EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini555 – 60147EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini638 – 69356VWFC 2PROSITE-ProRule annotationAdd
BLAST
Domaini698 – 75659VWFC 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 6 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 3 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG253557.
HOVERGENiHBG004805.
InParanoidiQ62918.
PhylomeDBiQ62918.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024731. EGF_dom_MSP1-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR001791. Laminin_G.
IPR001007. VWF_C.
[Graphical view]
PfamiPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 3 hits.
PF02210. Laminin_G_2. 1 hit.
PF00093. VWC. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 3 hits.
SM00282. LamG. 1 hit.
SM00210. TSPN. 1 hit.
SM00214. VWC. 3 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 6 hits.
PS01187. EGF_CA. 3 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62918-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRVLLRTF CVILGLEAVW GLGVDPSLQI DVLSELELGE STAGVRQVPG
60 70 80 90 100
LHNGTKAFLF QDSPRSIKAP IATAERFFQK LRNKHEFTIL VTLKQIHLNS
110 120 130 140 150
GVILSIHHLD HRYLELESSG HRNEIRLHYR SGTHRPHTEV FPYILADAKW
160 170 180 190 200
HKLSLAFSAS HLILHIDCNK IYERVVEMPS TDLPLGTTFW LGQRNNAHGY
210 220 230 240 250
FKGIMQDVQL LVMPQGFIAQ CPDLNRTCPT CNDFHGLVQK IMELQDILSK
260 270 280 290 300
TSAKLSRAEQ RMNRLDQCYC ERTCTMKGAT YREFESWTDG CKNCTCLNGT
310 320 330 340 350
IQCETLVCPA PDCPAKSAPA YVDGKCCKEC KSTCQFQGRS YFEGERSTVF
360 370 380 390 400
SASGMCVLYE CKDQTMKLVE NAGCPALDCP ESHQIALSHS CCKVCKGYDF
410 420 430 440 450
CSEKHTCMEN SVCRNLNDRA VCSCRDGFRA LREDNAYCED IDECAEGRHY
460 470 480 490 500
CRENTMCVNT PGSFLCICQT GYIRIDDYSC TEHDECLTNQ HNCDENALCF
510 520 530 540 550
NTVGGHNCVC KPGYTGNGTT CKAFCKDGCK NGGACIAANV CACPQGFTGP
560 570 580 590 600
SCETDIDECS EGFVQCDSRA NCINLPGWYH CECRDGYHDN GMFAPGGESC
610 620 630 640 650
EDIDECGTGR HSCANDTICF NLDGGYDCRC PHGKNCTGDC VHDGKVKHNG
660 670 680 690 700
QIWVLENDRC SVCSCQTGFV MCQRMVCDCE NPTVDLSCCP ECDPRLSSQC
710 720 730 740 750
LHQNGETVYN SGDTWAQDCR QCRCLQEEVD CWPLACPEVE CEFSVLPENE
760 770 780 790 800
CCPRCVTDPC QADTIRNDIT KTCLDEMNVV RFTGSSWIKH GTECTLCQCK
810
NGHVCCSVDP QCLQEL
Length:816
Mass (Da):90,953
Last modified:November 1, 1996 - v1
Checksum:iA999F76078060D6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48245 mRNA. Translation: AAC72245.1.
UniGeneiRn.11567.

Genome annotation databases

UCSCiRGD:620999. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48245 mRNA. Translation: AAC72245.1.
UniGeneiRn.11567.

3D structure databases

ProteinModelPortaliQ62918.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ62918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620999. rat.

Organism-specific databases

RGDi620999. Nell2.

Phylogenomic databases

eggNOGiNOG253557.
HOVERGENiHBG004805.
InParanoidiQ62918.
PhylomeDBiQ62918.

Miscellaneous databases

PROiQ62918.

Gene expression databases

GenevestigatoriQ62918.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024731. EGF_dom_MSP1-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR001791. Laminin_G.
IPR001007. VWF_C.
[Graphical view]
PfamiPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 3 hits.
PF02210. Laminin_G_2. 1 hit.
PF00093. VWC. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 3 hits.
SM00282. LamG. 1 hit.
SM00210. TSPN. 1 hit.
SM00214. VWC. 3 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 6 hits.
PS01187. EGF_CA. 3 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Biochemical characterization and expression analysis of neural thrombospondin-1-like proteins NELL1 and NELL2."
    Kuroda S., Oyasu M., Kawakami M., Kanayama N., Tanizawa K., Saito N., Abe T., Matsuhashi S., Ting K.
    Biochem. Biophys. Res. Commun. 265:79-86(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "A neuron-specific EGF family protein, NELL2, promotes survival of neurons through mitogen-activated protein kinases."
    Aihara K., Kuroda S., Kanayama N., Matsuyama S., Tanizawa K., Horie M.
    Brain Res. Mol. Brain Res. 116:86-93(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "NELL2, a neuron-specific EGF-like protein, is selectively expressed in glutamatergic neurons and contributes to the glutamatergic control of GnRH neurons at puberty."
    Ha C.M., Choi J., Choi E.J., Costa M.E., Lee B.J., Ojeda S.R.
    Neuroendocrinology 88:199-211(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNELL2_RAT
AccessioniPrimary (citable) accession number: Q62918
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.