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Protein

Peripheral plasma membrane protein CASK

Gene

Cask

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Note: Unlike other protein kinases, does not require a divalent cation such as magnesium for catalytic activity.By similarity

Enzyme regulationi

Differs from archetypal CaMK members in that the kinase domain exhibits a constitutively active conformation and the autoinhibitory region does not engage in direct contact with the ATP-binding cleft, although it still binds Ca2+/CAM.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei141 – 1411By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: RGD
  • calmodulin-dependent protein kinase activity Source: RGD
  • neurexin family protein binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • transcription coactivator activity Source: RGD

GO - Biological processi

  • intracellular protein transport Source: RGD
  • protein complex assembly Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.
ReactomeiR-RNO-3000170. Syndecan interactions.
R-RNO-373753. Nephrin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Peripheral plasma membrane protein CASK (EC:2.7.11.1)
Alternative name(s):
Calcium/calmodulin-dependent serine protein kinase
Gene namesi
Name:Cask
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62004. Cask.

Subcellular locationi

GO - Cellular componenti

  • ciliary membrane Source: BHF-UCL
  • cytoplasm Source: RGD
  • cytosol Source: Reactome
  • dendrite Source: RGD
  • membrane Source: RGD
  • nucleus Source: BHF-UCL
  • plasma membrane Source: RGD
  • presynaptic membrane Source: BHF-UCL
  • protein complex Source: RGD
  • synaptic membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 909909Peripheral plasma membrane protein CASKPRO_0000094570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei151 – 1511Phosphoserine; by autocatalysisBy similarity
Modified residuei155 – 1551Phosphoserine; by autocatalysisBy similarity
Modified residuei182 – 1821PhosphothreonineBy similarity
Modified residuei313 – 3131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62915.
PRIDEiQ62915.

PTM databases

iPTMnetiQ62915.
PhosphoSiteiQ62915.
SwissPalmiQ62915.

Expressioni

Tissue specificityi

Expressed in the foot process layer of podocytes in the kidney glomerulus and in tubules (at protein level). Detected in brain and neurons.3 Publications

Interactioni

Subunit structurei

Binds WHRN and NRXN1 cytosolic tail. Interacts with CASKIN1, APBA1, LIN7(A/B/C), and L27 domain of DLG1 and isoform 2 of DLG4. CASK and LIN7 form two mutually exclusive tripartite complexes with APBA1 or CASKIN1. Interacts with FCHSD2. Identified in a complex with ACTN4, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Interacts with TSPYL2. Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity). Interacts with KIRREL3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FCHSD2O948682EBI-704635,EBI-1215612From a different organism.

GO - Molecular functioni

  • calmodulin binding Source: RGD
  • neurexin family protein binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi248269. 9 interactions.
IntActiQ62915. 8 interactions.
MINTiMINT-220139.
STRINGi10116.ENSRNOP00000004187.

Structurei

Secondary structure

1
909
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni342 – 3443Combined sources
Helixi345 – 35915Combined sources
Helixi365 – 37612Combined sources
Helixi378 – 39114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RSONMR-B/D339-394[»]
ProteinModelPortaliQ62915.
SMRiQ62915. Positions 5-324, 339-394, 403-456, 487-572, 722-897.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62915.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 276265Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini343 – 39856L27 1PROSITE-ProRule annotationAdd
BLAST
Domaini402 – 45554L27 2PROSITE-ProRule annotationAdd
BLAST
Domaini489 – 56476PDZPROSITE-ProRule annotationAdd
BLAST
Domaini600 – 67071SH3PROSITE-ProRule annotationAdd
BLAST
Domaini722 – 894173Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni305 – 31511Calmodulin-bindingAdd
BLAST

Domaini

The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.By similarity
The protein kinase domain mediates the interaction with FCHSD2.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated
Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 2 L27 domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ62915.
KOiK06103.
PhylomeDBiQ62915.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR011009. Kinase-like_dom.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 2 hits.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS
60 70 80 90 100
SPGLSTEDLK REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC
110 120 130 140 150
FEIVKRADAG FVYSEAVASH YMRQILEALR YCHDNNIIHR DVKPHCVLLA
160 170 180 190 200
SKENSAPVKL GGFGVAIQLG ESGLVAGGRV GTPHFMAPEV VKREPYGKPV
210 220 230 240 250
DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR QWSHISESAK
260 270 280 290 300
DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA
310 320 330 340 350
RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV
360 370 380 390 400
LDSLEEIHAL TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN
410 420 430 440 450
PPSDAVQRAK EVLEEISCYP ENNDAKELKR ILTQPHFMAL LQTHHVVAHE
460 470 480 490 500
VYSDEALRVT PPPTSPYLNG DSPESANGDM DMENVTRVRL VQFQKNTDEP
510 520 530 540 550
MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN GISVANQTVE
560 570 580 590 600
QLQKMLREMR GSITFKIVPS YRTQSSSCER DSPSTSRQSP ANGHSSTNNS
610 620 630 640 650
VSIYVRAQFE YDPAKDDLIP CKEAGIRFRV GDIIQIISKD DHNWWQGKLE
660 670 680 690 700
NSKNGTAGLI PSPELQEWRV ACIAMEKTKQ EQQASCTWFG KKKKQYKDKY
710 720 730 740 750
LAKHNADLVT YEEVVKLPAF KRKTLVLLGA HGVGRRHIKN TLITKHPERF
760 770 780 790 800
AYPIPHTTRP PKKDEENGKN YYFVSHDQMM QDISNNEYLE YGSHEDAMYG
810 820 830 840 850
TKLETIRKIH EQGLIAILDV EPQALKVLRT AEFPPFVVFI AAPTITPGLN
860 870 880 890 900
EDESLQRLQK ESDVLQRTYA HYFDLTIINN EIDETIRHLE EAVELVCTAP

QWVPVSWVY
Length:909
Mass (Da):103,259
Last modified:November 1, 1997 - v1
Checksum:i3694A9A44AC29629
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47110 mRNA. Translation: AAB19127.1.
RefSeqiNP_071520.1. NM_022184.1.
UniGeneiRn.72627.

Genome annotation databases

GeneIDi29647.
KEGGirno:29647.
UCSCiRGD:62004. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47110 mRNA. Translation: AAB19127.1.
RefSeqiNP_071520.1. NM_022184.1.
UniGeneiRn.72627.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RSONMR-B/D339-394[»]
ProteinModelPortaliQ62915.
SMRiQ62915. Positions 5-324, 339-394, 403-456, 487-572, 722-897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248269. 9 interactions.
IntActiQ62915. 8 interactions.
MINTiMINT-220139.
STRINGi10116.ENSRNOP00000004187.

PTM databases

iPTMnetiQ62915.
PhosphoSiteiQ62915.
SwissPalmiQ62915.

Proteomic databases

PaxDbiQ62915.
PRIDEiQ62915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29647.
KEGGirno:29647.
UCSCiRGD:62004. rat.

Organism-specific databases

CTDi8573.
RGDi62004. Cask.

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ62915.
KOiK06103.
PhylomeDBiQ62915.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.
ReactomeiR-RNO-3000170. Syndecan interactions.
R-RNO-373753. Nephrin interactions.

Miscellaneous databases

EvolutionaryTraceiQ62915.
PROiQ62915.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR011009. Kinase-like_dom.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 2 hits.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSKP_RAT
AccessioniPrimary (citable) accession number: Q62915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.