Synaptojanin-1 - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Synaptojanin-1
EC=3.1.3.36

Alternative name(s):
Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1

Gene names

Name:

Synj1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1574 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Subunit structure	Binds AMPH, SH3GL1, SH3GL2 and SH3GL3.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Isoform 1 is found in neonatal brain, and in a wide variety of adult non-neuronal tissues. Isoform 2 is expressed predominantly in the neurons, but is also found in all other tissues at much lower levels. Isoform 1 and isoform 2 are detected in the lung and heart. Isoform 1 is expressed at higher levels than isoform 2 in the testis and liver and both isoforms are not detected in the skeletal muscle. Isoform 3 with the 16-amino-acid insert is only found in the brain while isoform 3 without the 16-amino-acid insert is found in the lung.
Developmental stage	At embryonic day 12 (E12) only isoform 1 is seen while at E16 and E18 isoform 1 and isoform 2 are seen. In the adult brain expression of isoform 2 increases dramatically as compared with its expression in embryonic brain where as isoform 1 decreases to undetectable levels.
Domain	Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats By similarity. The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. Splicing of the SAC1 domain does not alter the catalytic activity of synaptojanin 1.
Sequence similarities	Belongs to the synaptojanin family. In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. Contains 1 RRM (RNA recognition motif) domain. Contains 1 SAC domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from expression pattern. Source: RGD positive regulation of gliogenesis Inferred from mutant phenotype. Source: RGD positive regulation of receptor-mediated endocytosis Inferred from mutant phenotype. Source: RGD response to cytokine stimulus Inferred from mutant phenotype. Source: RGD response to retinoic acid Inferred from mutant phenotype. Source: RGD
Cellular component	synaptosome Inferred from direct assay. Source: RGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW SH3 domain binding Inferred from direct assay Ref.1. Source: RGD nucleotide binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC protein C-terminus binding Inferred from physical interaction Ref.4. Source: RGD protein complex binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sh3gl2	O35179	2	EBI-1149123,EBI-1149197
Sh3gl3	O35180	2	EBI-1149123,EBI-1149266

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select] Note: A stop codon is created in position 1309 of isoform 1 due to alternative splicing.

Isoform 1 (identifier: Q62910-1) Also known as: 170 kDa; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62910-2) Also known as: 145 kDa; The sequence of this isoform differs from the canonical sequence as follows: 1309-1574: Missing.

Isoform 3 (identifier: Q62910-3) Also known as: Delta-SAC; The sequence of this isoform differs from the canonical sequence as follows: 1-400: Missing.

Isoform 4 (identifier: Q62910-4) Also known as: 170 kDa-16AA; The sequence of this isoform differs from the canonical sequence as follows: 1141-1156: Missing.

Isoform 5 (identifier: Q62910-5) Also known as: 145 kDa-16AA; The sequence of this isoform differs from the canonical sequence as follows: 1141-1156: Missing. 1309-1574: Missing.

Isoform 6 (identifier: Q62910-6) Also known as: Delta-SAC-16AA; The sequence of this isoform differs from the canonical sequence as follows: 1-400: Missing. 1141-1156: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1574

1574

Synaptojanin-1

PRO_0000209732

Regions

Domain

119 – 442

324

SAC

Domain

894 – 971

78

RRM

Repeat

1401 – 1403

3

1

Repeat

1410 – 1412

3

2

Repeat

1421 – 1423

3

Region

500 – 899

400

Catalytic Potential

Region

1401 – 1423

23

3 X 3 AA repeats of N-P-F

Compositional bias

1042 – 1285

244

Pro-rich

Compositional bias

1533 – 1549

17

Pro-rich

Amino acid modifications

Modified residue

784

1

Phosphotyrosine By similarity

Modified residue

786

1

Phosphotyrosine By similarity

Modified residue

830

1

Phosphoserine By similarity

Modified residue

1048

1

Phosphothreonine By similarity

Modified residue

1053

1

Phosphoserine By similarity

Modified residue

1147

1

Phosphoserine By similarity

Modified residue

1217

1

Phosphothreonine By similarity

Modified residue

1350

1

Phosphoserine By similarity

Modified residue

1354

1

Phosphothreonine By similarity

Modified residue

1399

1

Phosphoserine By similarity

Modified residue

1566

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 400

400

Missing in isoform 3 and isoform 6.

VSP_002684

Alternative sequence

1141 – 1156

16

Missing in isoform 4, isoform 5 and isoform 6.

VSP_002685

Alternative sequence

1309 – 1574

266

Missing in isoform 2 and isoform 5.

VSP_002686

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (170 kDa) [UniParc]. Last modified May 16, 2006. Version 3. Checksum: F24B90CE48F55508 Show »	FASTA	1,574	172,881
10 20 30 40 50 60 MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK GTYAKVLDAY 70 80 90 100 110 120 GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS TEFISLRVDA SDEDRISEVR 130 140 150 160 170 180 KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL 190 200 210 220 230 240 RLMCGGVEIR TIYAAHKQAK ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD 250 260 270 280 290 300 DCVSSFIQIR GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN 310 320 330 340 350 360 LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSV LKPQVQKFLD 370 380 390 400 410 420 YGFFYFDGSA VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE MLAKQLEALG LAEKPQLVTR 430 440 450 460 470 480 FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT RTIQNNFFDS SKQEAIDVLL 490 500 510 520 530 540 LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW 550 560 570 580 590 600 NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI 610 620 630 640 650 660 VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI RDVAVDTVKT 670 680 690 700 710 720 GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER NEDFVEIARK LSFPMGRMLF 730 740 750 760 770 780 SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL INQKNAGQIF RGFLEGKVTF 790 800 810 820 830 840 APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT 850 860 870 880 890 900 WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI 910 920 930 940 950 960 KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV TFLEGSSALN VLSLNGKELL 970 980 990 1000 1010 1020 NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG EDAEVSADFD MEGDVDDYSA 1030 1040 1050 1060 1070 1080 EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS APSLPIRPSR APSRTPGPLS 1090 1100 1110 1120 1130 1140 SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG 1150 1160 1170 1180 1190 1200 GVGAPPSPGV TRREMEAPKS PGTARKDNIG RNQPSPQAGL AGPGPSGYGA ARPTIPARAG 1210 1220 1230 1240 1250 1260 VISAPQSQAR VSAGRLTPES QSKPLETSKG PAVLPEPLKP QAAFPPQPSL PTPAQKLQDP 1270 1280 1290 1300 1310 1320 LVPIAAPMPP SIPQSNLETP PLPPPRSRSS QSLPSDSSPQ LQQEQPTGQQ VKINGACGVK 1330 1340 1350 1360 1370 1380 QEPTLKSDPF EDLSLSVLAV SKAQPSAQIS PVLTPDPKML IQLPSASQSK VNSLSSVSCM 1390 1400 1410 1420 1430 1440 LTMPPVPEQS KSQESVGSSA NPFPSLPTRN PFTDRTAAPG NPFRVQSQES EATSWLSKEE 1450 1460 1470 1480 1490 1500 PVSNSPFPPL MPLSHDMSKP SSSLDGFEDN FDLQSQSTVK TSNPKGWVTF DEDEDFPTKG 1510 1520 1530 1540 1550 1560 KSRSVYPDSL GNTAASFDDD WSKGTNVSFC VLPARRPPPP PPPVPLLPPG TTSSAGPSTT 1570 LSSKASPTLD FTER « Hide
Isoform 2 (145 kDa) [UniParc]. Checksum: 669540DA79036E60 Show »	FASTA	1,308	144,523
10 20 30 40 50 60 MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK GTYAKVLDAY 70 80 90 100 110 120 GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS TEFISLRVDA SDEDRISEVR 130 140 150 160 170 180 KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL 190 200 210 220 230 240 RLMCGGVEIR TIYAAHKQAK ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD 250 260 270 280 290 300 DCVSSFIQIR GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN 310 320 330 340 350 360 LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSV LKPQVQKFLD 370 380 390 400 410 420 YGFFYFDGSA VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE MLAKQLEALG LAEKPQLVTR 430 440 450 460 470 480 FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT RTIQNNFFDS SKQEAIDVLL 490 500 510 520 530 540 LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW 550 560 570 580 590 600 NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI 610 620 630 640 650 660 VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI RDVAVDTVKT 670 680 690 700 710 720 GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER NEDFVEIARK LSFPMGRMLF 730 740 750 760 770 780 SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL INQKNAGQIF RGFLEGKVTF 790 800 810 820 830 840 APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT 850 860 870 880 890 900 WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI 910 920 930 940 950 960 KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV TFLEGSSALN VLSLNGKELL 970 980 990 1000 1010 1020 NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG EDAEVSADFD MEGDVDDYSA 1030 1040 1050 1060 1070 1080 EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS APSLPIRPSR APSRTPGPLS 1090 1100 1110 1120 1130 1140 SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG 1150 1160 1170 1180 1190 1200 GVGAPPSPGV TRREMEAPKS PGTARKDNIG RNQPSPQAGL AGPGPSGYGA ARPTIPARAG 1210 1220 1230 1240 1250 1260 VISAPQSQAR VSAGRLTPES QSKPLETSKG PAVLPEPLKP QAAFPPQPSL PTPAQKLQDP 1270 1280 1290 1300 LVPIAAPMPP SIPQSNLETP PLPPPRSRSS QSLPSDSSPQ LQQEQPTG « Hide
Isoform 3 (Delta-SAC) [UniParc]. Checksum: ECCEBCC2058FF0D5 Show »	FASTA	1,174	127,788
10 20 30 40 50 60 MLAKQLEALG LAEKPQLVTR FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT 70 80 90 100 110 120 RTIQNNFFDS SKQEAIDVLL LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM 130 140 150 160 170 180 CENFYKYSKP KKIRVCVGTW NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK 190 200 210 220 230 240 PTDIFAIGFE EMVELNAGNI VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV 250 260 270 280 290 300 FIRPQHAPFI RDVAVDTVKT GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER 310 320 330 340 350 360 NEDFVEIARK LSFPMGRMLF SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL 370 380 390 400 410 420 INQKNAGQIF RGFLEGKVTF APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS 430 440 450 460 470 480 AEDLDLLNAS FQDESKILYT WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK 490 500 510 520 530 540 IYKEVIAVQG PPDGTVLVSI KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV 550 560 570 580 590 600 TFLEGSSALN VLSLNGKELL NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG 610 620 630 640 650 660 EDAEVSADFD MEGDVDDYSA EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS 670 680 690 700 710 720 APSLPIRPSR APSRTPGPLS SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP 730 740 750 760 770 780 QRPPPPSGAR SPAPARKEFG GVGAPPSPGV TRREMEAPKS PGTARKDNIG RNQPSPQAGL 790 800 810 820 830 840 AGPGPSGYGA ARPTIPARAG VISAPQSQAR VSAGRLTPES QSKPLETSKG PAVLPEPLKP 850 860 870 880 890 900 QAAFPPQPSL PTPAQKLQDP LVPIAAPMPP SIPQSNLETP PLPPPRSRSS QSLPSDSSPQ 910 920 930 940 950 960 LQQEQPTGQQ VKINGACGVK QEPTLKSDPF EDLSLSVLAV SKAQPSAQIS PVLTPDPKML 970 980 990 1000 1010 1020 IQLPSASQSK VNSLSSVSCM LTMPPVPEQS KSQESVGSSA NPFPSLPTRN PFTDRTAAPG 1030 1040 1050 1060 1070 1080 NPFRVQSQES EATSWLSKEE PVSNSPFPPL MPLSHDMSKP SSSLDGFEDN FDLQSQSTVK 1090 1100 1110 1120 1130 1140 TSNPKGWVTF DEDEDFPTKG KSRSVYPDSL GNTAASFDDD WSKGTNVSFC VLPARRPPPP 1150 1160 1170 PPPVPLLPPG TTSSAGPSTT LSSKASPTLD FTER « Hide
Isoform 4 (170 kDa-16AA) [UniParc]. Checksum: 766CF67A85C9C89D Show »	FASTA	1,558	171,259
10 20 30 40 50 60 MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK GTYAKVLDAY 70 80 90 100 110 120 GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS TEFISLRVDA SDEDRISEVR 130 140 150 160 170 180 KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL 190 200 210 220 230 240 RLMCGGVEIR TIYAAHKQAK ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD 250 260 270 280 290 300 DCVSSFIQIR GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN 310 320 330 340 350 360 LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSV LKPQVQKFLD 370 380 390 400 410 420 YGFFYFDGSA VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE MLAKQLEALG LAEKPQLVTR 430 440 450 460 470 480 FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT RTIQNNFFDS SKQEAIDVLL 490 500 510 520 530 540 LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW 550 560 570 580 590 600 NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI 610 620 630 640 650 660 VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI RDVAVDTVKT 670 680 690 700 710 720 GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER NEDFVEIARK LSFPMGRMLF 730 740 750 760 770 780 SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL INQKNAGQIF RGFLEGKVTF 790 800 810 820 830 840 APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT 850 860 870 880 890 900 WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI 910 920 930 940 950 960 KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV TFLEGSSALN VLSLNGKELL 970 980 990 1000 1010 1020 NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG EDAEVSADFD MEGDVDDYSA 1030 1040 1050 1060 1070 1080 EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS APSLPIRPSR APSRTPGPLS 1090 1100 1110 1120 1130 1140 SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG 1150 1160 1170 1180 1190 1200 APKSPGTARK DNIGRNQPSP QAGLAGPGPS GYGAARPTIP ARAGVISAPQ SQARVSAGRL 1210 1220 1230 1240 1250 1260 TPESQSKPLE TSKGPAVLPE PLKPQAAFPP QPSLPTPAQK LQDPLVPIAA PMPPSIPQSN 1270 1280 1290 1300 1310 1320 LETPPLPPPR SRSSQSLPSD SSPQLQQEQP TGQQVKINGA CGVKQEPTLK SDPFEDLSLS 1330 1340 1350 1360 1370 1380 VLAVSKAQPS AQISPVLTPD PKMLIQLPSA SQSKVNSLSS VSCMLTMPPV PEQSKSQESV 1390 1400 1410 1420 1430 1440 GSSANPFPSL PTRNPFTDRT AAPGNPFRVQ SQESEATSWL SKEEPVSNSP FPPLMPLSHD 1450 1460 1470 1480 1490 1500 MSKPSSSLDG FEDNFDLQSQ STVKTSNPKG WVTFDEDEDF PTKGKSRSVY PDSLGNTAAS 1510 1520 1530 1540 1550 FDDDWSKGTN VSFCVLPARR PPPPPPPVPL LPPGTTSSAG PSTTLSSKAS PTLDFTER « Hide
Isoform 5 (145 kDa-16AA) [UniParc]. Checksum: 476102B8F6659A03 Show »	FASTA	1,292	142,901
10 20 30 40 50 60 MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK GTYAKVLDAY 70 80 90 100 110 120 GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS TEFISLRVDA SDEDRISEVR 130 140 150 160 170 180 KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL 190 200 210 220 230 240 RLMCGGVEIR TIYAAHKQAK ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD 250 260 270 280 290 300 DCVSSFIQIR GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN 310 320 330 340 350 360 LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSV LKPQVQKFLD 370 380 390 400 410 420 YGFFYFDGSA VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE MLAKQLEALG LAEKPQLVTR 430 440 450 460 470 480 FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT RTIQNNFFDS SKQEAIDVLL 490 500 510 520 530 540 LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW 550 560 570 580 590 600 NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI 610 620 630 640 650 660 VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI RDVAVDTVKT 670 680 690 700 710 720 GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER NEDFVEIARK LSFPMGRMLF 730 740 750 760 770 780 SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL INQKNAGQIF RGFLEGKVTF 790 800 810 820 830 840 APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT 850 860 870 880 890 900 WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI 910 920 930 940 950 960 KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV TFLEGSSALN VLSLNGKELL 970 980 990 1000 1010 1020 NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG EDAEVSADFD MEGDVDDYSA 1030 1040 1050 1060 1070 1080 EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS APSLPIRPSR APSRTPGPLS 1090 1100 1110 1120 1130 1140 SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG 1150 1160 1170 1180 1190 1200 APKSPGTARK DNIGRNQPSP QAGLAGPGPS GYGAARPTIP ARAGVISAPQ SQARVSAGRL 1210 1220 1230 1240 1250 1260 TPESQSKPLE TSKGPAVLPE PLKPQAAFPP QPSLPTPAQK LQDPLVPIAA PMPPSIPQSN 1270 1280 1290 LETPPLPPPR SRSSQSLPSD SSPQLQQEQP TG « Hide
Isoform 6 (Delta-SAC-16AA) [UniParc]. Checksum: 49BD3EA9920B1247 Show »	FASTA	1,158	126,166
10 20 30 40 50 60 MLAKQLEALG LAEKPQLVTR FQEVFRSMWS VNGDSISKIY AGTGALEGKA KLKDGARSVT 70 80 90 100 110 120 RTIQNNFFDS SKQEAIDVLL LGNTLNSDLA DKARALLTTG SLRVSEQTLQ SASSKVLKNM 130 140 150 160 170 180 CENFYKYSKP KKIRVCVGTW NVNGGKQFRS IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK 190 200 210 220 230 240 PTDIFAIGFE EMVELNAGNI VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV 250 260 270 280 290 300 FIRPQHAPFI RDVAVDTVKT GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER 310 320 330 340 350 360 NEDFVEIARK LSFPMGRMLF SHDYVFWCGD FNYRIDLPNE EVKELIRQQN WDSLIAGDQL 370 380 390 400 410 420 INQKNAGQIF RGFLEGKVTF APTYKYDLFS EDYDTSEKCR TPAWTDRVLW RRRKWPFDRS 430 440 450 460 470 480 AEDLDLLNAS FQDESKILYT WTPGTLLHYG RAELKTSDHR PVVALIDIDI FEVEAEERQK 490 500 510 520 530 540 IYKEVIAVQG PPDGTVLVSI KSSAQENTFF DDALIDELLQ QFAHFGEVIL IRFVEDKMWV 550 560 570 580 590 600 TFLEGSSALN VLSLNGKELL NRTITITLKS PDWIKTLEEE MSLEKISVTL PSSTSSTLLG 610 620 630 640 650 660 EDAEVSADFD MEGDVDDYSA EVEELLPQHL QPSSSSGLGT SPSSSPRTSP CQSPTAPEYS 670 680 690 700 710 720 APSLPIRPSR APSRTPGPLS SQGAPVDTQP AAQKESSQTI EPKRPPPPRP VAPPARPAPP 730 740 750 760 770 780 QRPPPPSGAR SPAPARKEFG APKSPGTARK DNIGRNQPSP QAGLAGPGPS GYGAARPTIP 790 800 810 820 830 840 ARAGVISAPQ SQARVSAGRL TPESQSKPLE TSKGPAVLPE PLKPQAAFPP QPSLPTPAQK 850 860 870 880 890 900 LQDPLVPIAA PMPPSIPQSN LETPPLPPPR SRSSQSLPSD SSPQLQQEQP TGQQVKINGA 910 920 930 940 950 960 CGVKQEPTLK SDPFEDLSLS VLAVSKAQPS AQISPVLTPD PKMLIQLPSA SQSKVNSLSS 970 980 990 1000 1010 1020 VSCMLTMPPV PEQSKSQESV GSSANPFPSL PTRNPFTDRT AAPGNPFRVQ SQESEATSWL 1030 1040 1050 1060 1070 1080 SKEEPVSNSP FPPLMPLSHD MSKPSSSLDG FEDNFDLQSQ STVKTSNPKG WVTFDEDEDF 1090 1100 1110 1120 1130 1140 PTKGKSRSVY PDSLGNTAAS FDDDWSKGTN VSFCVLPARR PPPPPPPVPL LPPGTTSSAG 1150 PSTTLSSKAS PTLDFTER « Hide

References

[1]	"A presynaptic inositol-5-phosphatase." McPherson P.S., Garcia E.P., Slepnev V.I., David C., Zhang X., Grabs D., Sossin W.S., Bauerfeind R., Nemoto Y., De Camilli P. Nature 379:353-357(1996) [PubMed: 8552192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 4 AND 5). Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Identification and characterisation of a novel splice variant of synaptojanin1." Woscholski R., Finan P.M., Radley E., Parker P.J. FEBS Lett. 432:5-8(1998) [PubMed: 9710239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties." Ramjaun A.R., McPherson P.S. J. Biol. Chem. 271:24856-24861(1996) [PubMed: 8798761] [Abstract] Cited for: ALTERNATIVE SPLICING. Tissue: Brain.
[4]	"The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain." Ringstad N., Nemoto Y., De Camilli P. Proc. Natl. Acad. Sci. U.S.A. 94:8569-8574(1997) [PubMed: 9238017] [Abstract] Cited for: INTERACTION WITH SH3GL1; SH3GL2 AND SH3GL3. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U45479 mRNA. Translation: AAB60525.2. U91836 mRNA. Translation: AAO24807.1. AJ006855 mRNA. Translation: CAA07267.1. Different termination.
IPI	IPI00210153. IPI00231602. IPI00231603. IPI00231604. IPI00231605. IPI00231606.
PIR	S68448.
RefSeq	NP_445928.2. NM_053476.2.
UniGene	Rn.22685.
3D structure databases
ProteinModelPortal	Q62910.
SMR	Q62910. Positions 895-971.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62910. 6 interactions.
MINT	MINT-128573.
STRING	Q62910.
PTM databases
PhosphoSite	Q62910.
Proteomic databases
PRIDE	Q62910.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000002812; ENSRNOP00000002812; ENSRNOG00000002051. ENSRNOT00000068229; ENSRNOP00000061753; ENSRNOG00000002051.
GeneID	85238.
KEGG	rno:85238.
UCSC	U45479. rat.
Organism-specific databases
CTD	8867.
RGD	69434. Synj1.
Phylogenomic databases
eggNOG	roNOG05346.
GeneTree	ENSGT00600000084387.
HOVERGEN	HBG079225.
InParanoid	Q62910.
PhylomeDB	Q62910.
Gene expression databases
ArrayExpress	Q62910.
Genevestigator	Q62910.
GermOnline	ENSRNOG00000002051. Rattus norvegicus.
Family and domain databases
InterPro	IPR015047. DUF1866. IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR002013. Syja_N. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
KO	K01099.
Pfam	PF08952. DUF1866. 1 hit. PF03372. Exo_endo_phos. 1 hit. PF02383. Syja_N. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS50102. RRM. 1 hit. PS50275. SAC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNJ1_RAT

Accession

Primary (citable) accession number: Q62910
Secondary accession number(s): O89092, Q62911, Q810Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 16, 2006
Last modified:	November 16, 2011
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents