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Protein

Kinesin-like protein KIF2C

Gene

Kif2c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei210 – 2101ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi294 – 3018ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity Source: GO_Central
  2. ATP binding Source: UniProtKB-KW
  3. microtubule motor activity Source: GO_Central
  4. microtubule plus-end binding Source: UniProtKB

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. metabolic process Source: GOC
  3. microtubule-based movement Source: GO_Central
  4. microtubule depolymerization Source: GO_Central
  5. mitotic metaphase plate congression Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF2C
Alternative name(s):
Kinesin-related protein 2
Mitotic centromere-associated kinesin
Short name:
MCAK
Gene namesi
Name:Kif2c
Synonyms:Krp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620239. Kif2c.

Subcellular locationi

Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2 (By similarity).By similarity

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-KW
  3. kinesin complex Source: GO_Central
  4. microtubule Source: UniProtKB-KW
  5. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 671671Kinesin-like protein KIF2CPRO_0000125421Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411Phosphoserine; by AURKBBy similarity
Modified residuei55 – 551PhosphoserineBy similarity
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei61 – 611PhosphoserineBy similarity
Modified residuei112 – 1121PhosphoserineBy similarity
Modified residuei138 – 1381PhosphoserineBy similarity
Modified residuei576 – 5761PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62909.
PRIDEiQ62909.

PTM databases

PhosphoSiteiQ62909.

Expressioni

Tissue specificityi

Testis. Localized to the meiotically active cells of the seminiferous epithelia in the testis.

Gene expression databases

GenevestigatoriQ62909.

Interactioni

Subunit structurei

Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends (By similarity). Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025903.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 534331Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 200200GlobularSequence AnalysisAdd
BLAST
Regioni153 – 18432Negative regulator of microtubule-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili566 – 60136Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 474Microtubule tip localization signal

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
HOGENOMiHOG000231329.
HOVERGENiHBG003875.
InParanoidiQ62909.
KOiK10393.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDIDDVAAI NPELVQLLPL RPKDSLPLQE NVTIPKQKRK SVNSKIPGPK
60 70 80 90 100
EGLRSRSTRI STVSEVRIPA QENEMEVELP VSTNSRKPFP IHTGHPRPSC
110 120 130 140 150
STVTELPLLM ISEEAEEQAH STRSTSSANP GNSVRRKSCI VKEMEKMKNK
160 170 180 190 200
REEKRAQNSE IRIKRAQEYD NSFPNWEFAR MIKEFRVTMD CNPLTVTDPI
210 220 230 240 250
EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP KLKVDLTKYL
260 270 280 290 300
ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK
310 320 330 340 350
THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRSLN LEVYVTFFEI
360 370 380 390 400
YNGKVFELLN KKAKLRVLED SKQQVQVVGL QEYLVTCADD VIKMINMGSA
410 420 430 440 450
CRTSGQTFAN SNSSRSHACF QILLRAKGRL HGKFSLVDLA GNERGADTSS
460 470 480 490 500
ADRQTRMEGA EINKSLLALK ESIRALGQNK AHTPFRESKL TQVLRDSFIG
510 520 530 540 550
ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP SGEQAVQMET
560 570 580 590 600
EEMDASSHGA SLTGNEEEEL SSQMSSFNEA MTQIRELEER AMEELREIIQ
610 620 630 640 650
QGPSWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALQEVIKAL
660 670
RLAMQLEEQA SKQINSKKRH Q
Length:671
Mass (Da):75,572
Last modified:April 20, 2010 - v3
Checksum:iD3CE21F711F88506
GO

Sequence cautioni

The sequence AAC53528.1 differs from that shown. Reason: Frameshift at positions 26 and 56. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44979 mRNA. Translation: AAC53528.1. Frameshift.
PIRiT10755.
RefSeqiNP_608302.2. NM_134472.3.
UniGeneiRn.219966.

Genome annotation databases

GeneIDi171529.
KEGGirno:171529.
UCSCiRGD:620239. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44979 mRNA. Translation: AAC53528.1. Frameshift.
PIRiT10755.
RefSeqiNP_608302.2. NM_134472.3.
UniGeneiRn.219966.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025903.

PTM databases

PhosphoSiteiQ62909.

Proteomic databases

PaxDbiQ62909.
PRIDEiQ62909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171529.
KEGGirno:171529.
UCSCiRGD:620239. rat.

Organism-specific databases

CTDi11004.
RGDi620239. Kif2c.

Phylogenomic databases

eggNOGiCOG5059.
HOGENOMiHOG000231329.
HOVERGENiHBG003875.
InParanoidiQ62909.
KOiK10393.

Miscellaneous databases

NextBioi622515.

Gene expression databases

GenevestigatoriQ62909.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis."
    Sperry A.O., Zhao L.-P.
    Mol. Biol. Cell 7:289-305(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. Sperry A.O.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiKIF2C_RAT
AccessioniPrimary (citable) accession number: Q62909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 20, 2010
Last modified: March 4, 2015
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.