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Q62909

- KIF2C_RAT

UniProt

Q62909 - KIF2C_RAT

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Protein
Kinesin-like protein KIF2C
Gene
Kif2c, Krp2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells By similarity. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei210 – 2101ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi294 – 3018ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule motor activity Source: InterPro
  3. microtubule plus-end binding Source: UniProtKB

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-KW
  2. microtubule-based movement Source: InterPro
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF2C
Alternative name(s):
Kinesin-related protein 2
Mitotic centromere-associated kinesin
Short name:
MCAK
Gene namesi
Name:Kif2c
Synonyms:Krp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620239. Kif2c.

Subcellular locationi

Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B By similarity. Centromeric localization requires the presence of BUB1 and SGOL2 By similarity.

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-KW
  3. kinesin complex Source: InterPro
  4. microtubule Source: UniProtKB-KW
  5. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 671671Kinesin-like protein KIF2C
PRO_0000125421Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411Phosphoserine; by AURKB By similarity
Modified residuei55 – 551Phosphoserine By similarity
Modified residuei57 – 571Phosphoserine By similarity
Modified residuei61 – 611Phosphoserine By similarity
Modified residuei112 – 1121Phosphoserine By similarity
Modified residuei138 – 1381Phosphoserine By similarity
Modified residuei576 – 5761Phosphoserine By similarity

Post-translational modificationi

Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity By similarity.
Ubiquitinated By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62909.
PRIDEiQ62909.

PTM databases

PhosphoSiteiQ62909.

Expressioni

Tissue specificityi

Testis. Localized to the meiotically active cells of the seminiferous epithelia in the testis.

Gene expression databases

GenevestigatoriQ62909.

Interactioni

Subunit structurei

Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends By similarity. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025903.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 534331Kinesin motor
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 200200Globular Reviewed prediction
Add
BLAST
Regioni153 – 18432Negative regulator of microtubule-binding By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili566 – 60136 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 474Microtubule tip localization signal

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends By similarity.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
HOGENOMiHOG000231329.
HOVERGENiHBG003875.
KOiK10393.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62909-1 [UniParc]FASTAAdd to Basket

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MIDIDDVAAI NPELVQLLPL RPKDSLPLQE NVTIPKQKRK SVNSKIPGPK    50
EGLRSRSTRI STVSEVRIPA QENEMEVELP VSTNSRKPFP IHTGHPRPSC 100
STVTELPLLM ISEEAEEQAH STRSTSSANP GNSVRRKSCI VKEMEKMKNK 150
REEKRAQNSE IRIKRAQEYD NSFPNWEFAR MIKEFRVTMD CNPLTVTDPI 200
EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP KLKVDLTKYL 250
ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK 300
THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRSLN LEVYVTFFEI 350
YNGKVFELLN KKAKLRVLED SKQQVQVVGL QEYLVTCADD VIKMINMGSA 400
CRTSGQTFAN SNSSRSHACF QILLRAKGRL HGKFSLVDLA GNERGADTSS 450
ADRQTRMEGA EINKSLLALK ESIRALGQNK AHTPFRESKL TQVLRDSFIG 500
ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP SGEQAVQMET 550
EEMDASSHGA SLTGNEEEEL SSQMSSFNEA MTQIRELEER AMEELREIIQ 600
QGPSWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALQEVIKAL 650
RLAMQLEEQA SKQINSKKRH Q 671
Length:671
Mass (Da):75,572
Last modified:April 20, 2010 - v3
Checksum:iD3CE21F711F88506
GO

Sequence cautioni

The sequence AAC53528.1 differs from that shown. Reason: Frameshift at positions 26 and 56.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U44979 mRNA. Translation: AAC53528.1. Frameshift.
PIRiT10755.
RefSeqiNP_608302.2. NM_134472.3.
UniGeneiRn.219966.

Genome annotation databases

GeneIDi171529.
KEGGirno:171529.
UCSCiRGD:620239. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U44979 mRNA. Translation: AAC53528.1 . Frameshift.
PIRi T10755.
RefSeqi NP_608302.2. NM_134472.3.
UniGenei Rn.219966.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025903.

PTM databases

PhosphoSitei Q62909.

Proteomic databases

PaxDbi Q62909.
PRIDEi Q62909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 171529.
KEGGi rno:171529.
UCSCi RGD:620239. rat.

Organism-specific databases

CTDi 11004.
RGDi 620239. Kif2c.

Phylogenomic databases

eggNOGi COG5059.
HOGENOMi HOG000231329.
HOVERGENi HBG003875.
KOi K10393.

Miscellaneous databases

NextBioi 622515.

Gene expression databases

Genevestigatori Q62909.

Family and domain databases

Gene3Di 3.40.850.10. 1 hit.
InterProi IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
Pfami PF00225. Kinesin. 1 hit.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis."
    Sperry A.O., Zhao L.-P.
    Mol. Biol. Cell 7:289-305(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. Sperry A.O.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiKIF2C_RAT
AccessioniPrimary (citable) accession number: Q62909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 20, 2010
Last modified: September 3, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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