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Q62909 (KIF2C_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF2C
Alternative name(s):
Kinesin-related protein 2
Mitotic centromere-associated kinesin
Short name=MCAK
Gene names
Name:Kif2c
Synonyms:Krp2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells By similarity. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis By similarity.

Subunit structure

Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends By similarity. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding By similarity.

Subcellular location

Cytoplasm › cytoskeleton By similarity. Nucleus By similarity. Chromosome › centromere By similarity. Chromosome › centromere › kinetochore By similarity. Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B By similarity. Centromeric localization requires the presence of BUB1 and SGOL2 By similarity.

Tissue specificity

Testis. Localized to the meiotically active cells of the seminiferous epithelia in the testis.

Domain

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends By similarity.

Post-translational modification

Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity By similarity.

Sequence similarities

Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.

Contains 1 kinesin-motor domain.

Sequence caution

The sequence AAC53528.1 differs from that shown. Reason: Frameshift at positions 26 and 56.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 671671Kinesin-like protein KIF2C
PRO_0000125421

Regions

Domain201 – 464264Kinesin-motor
Nucleotide binding294 – 3018ATP By similarity
Region1 – 200200Globular Potential
Region153 – 18432Negative regulator of microtubule-binding By similarity
Coiled coil566 – 60136 Potential
Motif44 – 474Microtubule tip localization signal

Sites

Binding site2101ATP By similarity

Amino acid modifications

Modified residue411Phosphoserine; by AURKB By similarity
Modified residue551Phosphoserine By similarity
Modified residue571Phosphoserine By similarity
Modified residue611Phosphoserine By similarity
Modified residue1121Phosphoserine By similarity
Modified residue1381Phosphoserine By similarity
Modified residue5761Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q62909 [UniParc].

Last modified April 20, 2010. Version 3.
Checksum: D3CE21F711F88506

FASTA67175,572
        10         20         30         40         50         60 
MIDIDDVAAI NPELVQLLPL RPKDSLPLQE NVTIPKQKRK SVNSKIPGPK EGLRSRSTRI 

        70         80         90        100        110        120 
STVSEVRIPA QENEMEVELP VSTNSRKPFP IHTGHPRPSC STVTELPLLM ISEEAEEQAH 

       130        140        150        160        170        180 
STRSTSSANP GNSVRRKSCI VKEMEKMKNK REEKRAQNSE IRIKRAQEYD NSFPNWEFAR 

       190        200        210        220        230        240 
MIKEFRVTMD CNPLTVTDPI EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP 

       250        260        270        280        290        300 
KLKVDLTKYL ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK 

       310        320        330        340        350        360 
THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRSLN LEVYVTFFEI YNGKVFELLN 

       370        380        390        400        410        420 
KKAKLRVLED SKQQVQVVGL QEYLVTCADD VIKMINMGSA CRTSGQTFAN SNSSRSHACF 

       430        440        450        460        470        480 
QILLRAKGRL HGKFSLVDLA GNERGADTSS ADRQTRMEGA EINKSLLALK ESIRALGQNK 

       490        500        510        520        530        540 
AHTPFRESKL TQVLRDSFIG ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP 

       550        560        570        580        590        600 
SGEQAVQMET EEMDASSHGA SLTGNEEEEL SSQMSSFNEA MTQIRELEER AMEELREIIQ 

       610        620        630        640        650        660 
QGPSWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALQEVIKAL RLAMQLEEQA 

       670 
SKQINSKKRH Q

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References

[1]"Kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis."
Sperry A.O., Zhao L.-P.
Mol. Biol. Cell 7:289-305(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[2]Sperry A.O.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U44979 mRNA. Translation: AAC53528.1. Frameshift.
IPIIPI00199226.
PIRT10755.
RefSeqNP_608302.2. NM_134472.3.
UniGeneRn.219966.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025903.

PTM databases

PhosphoSiteQ62909.

Proteomic databases

PaxDbQ62909.
PRIDEQ62909.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID171529.
KEGGrno:171529.
UCSCRGD:620239. rat.

Organism-specific databases

CTD11004.
RGD620239. Kif2c.

Phylogenomic databases

eggNOGCOG5059.
HOGENOMHOG000231329.
HOVERGENHBG003875.
KOK10393.

Gene expression databases

ArrayExpressQ62909.
GenevestigatorQ62909.
GermOnlineENSRNOG00000019100. Rattus norvegicus.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
[Graphical view]
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
PROSITEPS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622515.

Entry information

Entry nameKIF2C_RAT
AccessionPrimary (citable) accession number: Q62909
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 20, 2010
Last modified: April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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