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Q62909

- KIF2C_RAT

UniProt

Q62909 - KIF2C_RAT

Protein

Kinesin-like protein KIF2C

Gene

Kif2c

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei210 – 2101ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi294 – 3018ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: InterPro
    3. microtubule plus-end binding Source: UniProtKB

    GO - Biological processi

    1. chromosome segregation Source: UniProtKB-KW
    2. microtubule-based movement Source: InterPro
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KIF2C
    Alternative name(s):
    Kinesin-related protein 2
    Mitotic centromere-associated kinesin
    Short name:
    MCAK
    Gene namesi
    Name:Kif2c
    Synonyms:Krp2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620239. Kif2c.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
    Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B By similarity. Centromeric localization requires the presence of BUB1 and SGOL2 By similarity.By similarity

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-KW
    3. kinesin complex Source: InterPro
    4. microtubule Source: UniProtKB-KW
    5. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 671671Kinesin-like protein KIF2CPRO_0000125421Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411Phosphoserine; by AURKBBy similarity
    Modified residuei55 – 551PhosphoserineBy similarity
    Modified residuei57 – 571PhosphoserineBy similarity
    Modified residuei61 – 611PhosphoserineBy similarity
    Modified residuei112 – 1121PhosphoserineBy similarity
    Modified residuei138 – 1381PhosphoserineBy similarity
    Modified residuei576 – 5761PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.By similarity
    Ubiquitinated.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ62909.
    PRIDEiQ62909.

    PTM databases

    PhosphoSiteiQ62909.

    Expressioni

    Tissue specificityi

    Testis. Localized to the meiotically active cells of the seminiferous epithelia in the testis.

    Gene expression databases

    GenevestigatoriQ62909.

    Interactioni

    Subunit structurei

    Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends By similarity. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025903.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini204 – 534331Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 200200GlobularSequence AnalysisAdd
    BLAST
    Regioni153 – 18432Negative regulator of microtubule-bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili566 – 60136Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi44 – 474Microtubule tip localization signal

    Domaini

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    HOGENOMiHOG000231329.
    HOVERGENiHBG003875.
    KOiK10393.

    Family and domain databases

    Gene3Di3.40.850.10. 1 hit.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62909-1 [UniParc]FASTAAdd to Basket

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    MIDIDDVAAI NPELVQLLPL RPKDSLPLQE NVTIPKQKRK SVNSKIPGPK    50
    EGLRSRSTRI STVSEVRIPA QENEMEVELP VSTNSRKPFP IHTGHPRPSC 100
    STVTELPLLM ISEEAEEQAH STRSTSSANP GNSVRRKSCI VKEMEKMKNK 150
    REEKRAQNSE IRIKRAQEYD NSFPNWEFAR MIKEFRVTMD CNPLTVTDPI 200
    EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP KLKVDLTKYL 250
    ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK 300
    THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRSLN LEVYVTFFEI 350
    YNGKVFELLN KKAKLRVLED SKQQVQVVGL QEYLVTCADD VIKMINMGSA 400
    CRTSGQTFAN SNSSRSHACF QILLRAKGRL HGKFSLVDLA GNERGADTSS 450
    ADRQTRMEGA EINKSLLALK ESIRALGQNK AHTPFRESKL TQVLRDSFIG 500
    ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP SGEQAVQMET 550
    EEMDASSHGA SLTGNEEEEL SSQMSSFNEA MTQIRELEER AMEELREIIQ 600
    QGPSWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALQEVIKAL 650
    RLAMQLEEQA SKQINSKKRH Q 671
    Length:671
    Mass (Da):75,572
    Last modified:April 20, 2010 - v3
    Checksum:iD3CE21F711F88506
    GO

    Sequence cautioni

    The sequence AAC53528.1 differs from that shown. Reason: Frameshift at positions 26 and 56.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44979 mRNA. Translation: AAC53528.1. Frameshift.
    PIRiT10755.
    RefSeqiNP_608302.2. NM_134472.3.
    UniGeneiRn.219966.

    Genome annotation databases

    GeneIDi171529.
    KEGGirno:171529.
    UCSCiRGD:620239. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44979 mRNA. Translation: AAC53528.1 . Frameshift.
    PIRi T10755.
    RefSeqi NP_608302.2. NM_134472.3.
    UniGenei Rn.219966.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025903.

    PTM databases

    PhosphoSitei Q62909.

    Proteomic databases

    PaxDbi Q62909.
    PRIDEi Q62909.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171529.
    KEGGi rno:171529.
    UCSCi RGD:620239. rat.

    Organism-specific databases

    CTDi 11004.
    RGDi 620239. Kif2c.

    Phylogenomic databases

    eggNOGi COG5059.
    HOGENOMi HOG000231329.
    HOVERGENi HBG003875.
    KOi K10393.

    Miscellaneous databases

    NextBioi 622515.

    Gene expression databases

    Genevestigatori Q62909.

    Family and domain databases

    Gene3Di 3.40.850.10. 1 hit.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis."
      Sperry A.O., Zhao L.-P.
      Mol. Biol. Cell 7:289-305(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. Sperry A.O.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiKIF2C_RAT
    AccessioniPrimary (citable) accession number: Q62909
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3