Reviewed, UniProtKB/Swiss-Prot Q62904 (DHB7_RAT)

Names and origin

Protein names

Recommended name:
    3-keto-steroid reductase
    EC=1.1.1.270
Alternative name(s):
    Estradiol 17-beta-dehydrogenase 7
    EC=1.1.1.62
    17-beta-hydroxysteroid dehydrogenase 7
      Short name=17-beta-HSD 7
    PRL receptor-associated protein
      Short name=PRAP

Gene names

Name:

Hsd17b7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH. Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis. Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Subunit structure	Binds to the short form of prolactin receptor.
Subcellular location	Cell membrane; Single-pass membrane protein.
Tissue specificity	Most abundant in ovaries of pregnant animals.
Post-translational modification	Phosphorylated.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
PTM	Acetylation Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	female pregnancy Ref.1 Inferred from physical interaction. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Ref.1 Inferred from physical interaction. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC prolactin receptor binding Ref.1 Inferred from mutant phenotype. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	3-keto-steroid reductase		PRO_0000054588
Regions
	Topological domain	1 – 229	229	Extracellular Potential
	Transmembrane	230 – 250	21	Potential
	Topological domain	251 – 334	84	Cytoplasmic Potential
	Nucleotide binding	8 – 15	8	NAD Potential
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	171	1	Substrate By similarity
Amino acid modifications
	Modified residue	321	1	N6-acetyllysine By similarity
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q62904-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 448B546CE8FCBFAC	FASTA	334	37,372
	10 20 30 40 50 60 MRKVVLITGA SSGIGLALCG RLLAEDDDLH LCLACRNLSK AGAVRDALLA SHPSAEVSIV 70 80 90 100 110 120 QMDVSNLQSV VRGAEEVKRR FQRLDYLYLN AGIMPNPQLN LKAFFCGIFS RNVIHMFSTA 130 140 150 160 170 180 EGLLTQNDKI TADGFQEVFE TNLFGHFILI RELEPLLCHS DNPSQLIWTS SRNAKKSNFS 190 200 210 220 230 240 LEDIQHAKGQ EPYSSSKYAT DLLNVALNRN FNQKGLYSSV TCPGVVMTNL TYGILPPFVW 250 260 270 280 290 300 TLLLPVIWLL RFFAHAFTVT PYNGAEALVW LFHQKPESLN PLTKYLSGTT GLGTNYVKGQ 310 320 330 KMDVDEDTAE KFYKTLLELE KQVRITIQKS DHHS

References

[1]	"Cloning and characterization of an ovarian-specific protein that associates with the short form of the prolactin receptor." Duan W.R., Linzer D.I.H., Gibori G. J. Biol. Chem. 271:15602-15607(1996) [PubMed: 8663045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-18. Strain: Sprague-Dawley. Tissue: Corpus luteum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U44803 mRNA. Translation: AAC52623.1.
IPI	IPI00327252.
RefSeq	NP_058931.1.
UniGene	Rn.7040
3D structure databases
SMR	Q62904. Positions 2-306.
ModBase	Search...
Protein-protein interaction databases
STRING	Q62904.
Proteomic databases
PRIDE	Q62904.
Genome annotation databases
Ensembl	ENSRNOT00000003812; ENSRNOP00000003812; ENSRNOG00000002826; Rattus norvegicus. [Genome view]
GeneID	29540.
KEGG	rno:29540.
UCSC	NM_017235. rat.
Organism-specific databases
CTD	29540.
RGD	2837. Hsd17b7.
Phylogenomic databases
eggNOG	roNOG15744.
HOVERGEN	Q62904.
InParanoid	Q62904.
PhylomeDB	Q62904.
Enzyme and pathway databases
BRENDA	1.1.1.270. 248. 1.1.1.62. 248.
Gene expression databases
ArrayExpress	Q62904.
Genevestigator	Q62904.
GermOnline	ENSRNOG00000002826. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609535.

Entry information

Entry name

DHB7_RAT

Accession

Primary (citable) accession number: Q62904

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)