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Q62897

- KCND3_RAT

UniProt

Q62897 - KCND3_RAT

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Protein

Potassium voltage-gated channel subfamily D member 3

Gene

Kcnd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi104 – 1041ZincBy similarity
Metal bindingi131 – 1311ZincBy similarity
Metal bindingi132 – 1321ZincBy similarity

GO - Molecular functioni

  1. A-type (transient outward) potassium channel activity Source: RGD
  2. ion channel activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. potassium channel activity Source: RGD
  5. voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  1. cellular response to BMP stimulus Source: RGD
  2. membrane repolarization Source: Ensembl
  3. potassium ion export Source: Ensembl
  4. potassium ion transport Source: RGD
  5. protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Metal-binding, Potassium, Zinc

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily D member 3
Alternative name(s):
Voltage-gated potassium channel subunit Kv4.3
Gene namesi
Name:Kcnd3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi68394. Kcnd3.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell membranesarcolemma. Cell projectiondendrite By similarity
Note: Interaction with palmitoylated KCNIP2 and KCNIP3 enhances cell surface expression.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 181181CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei182 – 20221Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei222 – 24221Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Topological domaini243 – 25614CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei257 – 27721Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei287 – 30721Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Topological domaini308 – 32013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei321 – 34121Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Intramembranei360 – 38021Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST
Transmembranei382 – 40221Helical; Name=Segment S6Sequence AnalysisAdd
BLAST
Topological domaini403 – 655253CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. dendrite Source: RGD
  2. integral component of plasma membrane Source: RGD
  3. neuronal cell body Source: RGD
  4. neuron projection Source: RGD
  5. perinuclear endoplasmic reticulum Source: RGD
  6. plasma membrane Source: RGD
  7. voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Potassium voltage-gated channel subfamily D member 3PRO_0000054071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei569 – 5691Phosphoserine; by CaMK2D1 Publication

Post-translational modificationi

Regulated through phosphorylation at Ser-569 by CaMK2D.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62897.

PTM databases

PhosphoSiteiQ62897.

Expressioni

Tissue specificityi

Highly expressed in brain, in particular in the retrosplenial cortex, medial habenula, anterior thalamus, hippocampus, cerebellum and lateral geniculate and superior colliculus. Highly expressed in heart atrium (at protein level) and throughout the ventricle wall, in lung and vas deferens.5 Publications

Gene expression databases

ExpressionAtlasiQ62897. baseline and differential.
GenevestigatoriQ62897.

Interactioni

Subunit structurei

Homotetramer or heterotetramer with KCND1 and/or KCND2. Interacts with DLG1. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Interacts with KCNE1, KCNE2, SCN1B and KCNAB1 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-29245N.
IntActiQ62897. 1 interaction.
MINTiMINT-103596.

Structurei

Secondary structure

1
655
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83Combined sources
Helixi9 – 168Combined sources
Beta strandi41 – 466Combined sources
Beta strandi49 – 546Combined sources
Helixi55 – 595Combined sources
Turni65 – 673Combined sources
Helixi70 – 745Combined sources
Turni77 – 804Combined sources
Beta strandi81 – 844Combined sources
Helixi88 – 9912Combined sources
Helixi111 – 12111Combined sources
Turni130 – 1323Combined sources
Helixi133 – 1386Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I2RX-ray3.35A/B/C/D/I/J/K/L2-143[»]
ProteinModelPortaliQ62897.
SMRiQ62897. Positions 3-140.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62897.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2019Interaction with KCNIP2By similarityAdd
BLAST
Regioni474 – 48916Mediates dendritic targetingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi367 – 3726Selectivity filterBy similarity

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231013.
HOVERGENiHBG106687.
InParanoidiQ62897.
KOiK04893.
PhylomeDBiQ62897.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004056. K_chnl_volt-dep_Kv4.3.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01518. KV43CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62897-1) [UniParc]FASTAAdd to Basket

Also known as: Kv4.3 long form

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR
60 70 80 90 100
FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT
110 120 130 140 150
GKLHYPRYEC ISAYDDELAF YGILPEIIGD CCYEEYKDRK RENAERLMDD
160 170 180 190 200
NESENNQESM PSLSFRQTMW RAFENPHTST LALVFYYVTG FFIAVSVITN
210 220 230 240 250
VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE YLLRLFAAPS
260 270 280 290 300
RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF
310 320 330 340 350
SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK
360 370 380 390 400
FTSIPASFWY TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP
410 420 430 440 450
VIVSNFSRIY HQNQRADKRR AQKKARLARI RVAKTGSSNA YLHSKRNGLL
460 470 480 490 500
NEALELTGTP EEEHMGKTTS LIESQHHHLL HCLEKTTGLS YLVDDPLLSV
510 520 530 540 550
RTSTIKNHEF IDEQMFEQNC MESSMQNYPS TRSPSLSSHS GLTTTCCSRR
560 570 580 590 600
SKKTTHLPNS NLPATRLRSM QELSTIHIQG SEQPSLTTSR SSLNLKADDG
610 620 630 640 650
LRPNCKTSQI TTAIISIPTP PALTPEGESR PPPASPGPNT NIPSITSNVV

KVSVL
Length:655
Mass (Da):73,513
Last modified:November 7, 2003 - v2
Checksum:i26BC512BDE069C09
GO
Isoform 2 (identifier: Q62897-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     488-506: Missing.

Show »
Length:636
Mass (Da):71,454
Checksum:iA2D65B7C3FA5A58A
GO
Isoform 3 (identifier: Q62897-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     488-506: Missing.
     608-655: SQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSITSNVVKVSVL → QDQEQPRGRVVTCKQEEIITLCI

Show »
Length:611
Mass (Da):69,294
Checksum:i0D6042030E46F4C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241L → H in AAA80459. (PubMed:9001401)Curated
Sequence conflicti404 – 4041S → T in AAC52695. (PubMed:8734615)Curated
Sequence conflicti404 – 4041S → T in AAK07651. (PubMed:11427525)Curated
Sequence conflicti569 – 5691S → T in AAA80459. (PubMed:9001401)Curated
Sequence conflicti631 – 6311P → A in AAC52695. (PubMed:8734615)Curated
Sequence conflicti654 – 6541V → A in AAB18337. (PubMed:8831489)Curated
Sequence conflicti654 – 6541V → A in BAA24525. (PubMed:9450548)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei488 – 50619Missing in isoform 2 and isoform 3. 3 PublicationsVSP_008831Add
BLAST
Alternative sequencei608 – 65548SQITT…KVSVL → QDQEQPRGRVVTCKQEEIIT LCI in isoform 3. 1 PublicationVSP_008832Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75448 mRNA. Translation: AAB18337.1.
U42975 mRNA. Translation: AAC52695.1.
L48619 mRNA. Translation: AAA80459.1.
AB003587 mRNA. Translation: BAA24525.1.
AF334791 mRNA. Translation: AAK07651.1.
U92897 mRNA. Translation: AAB53321.1.
RefSeqiNP_001257891.1. NM_001270962.1.
NP_001257892.1. NM_001270963.1.
NP_113927.2. NM_031739.3. [Q62897-3]
UniGeneiRn.10540.
Rn.214215.

Genome annotation databases

EnsembliENSRNOT00000019997; ENSRNOP00000019997; ENSRNOG00000014686.
ENSRNOT00000051835; ENSRNOP00000049111; ENSRNOG00000014686.
GeneIDi65195.
KEGGirno:65195.
UCSCiRGD:68394. rat. [Q62897-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75448 mRNA. Translation: AAB18337.1 .
U42975 mRNA. Translation: AAC52695.1 .
L48619 mRNA. Translation: AAA80459.1 .
AB003587 mRNA. Translation: BAA24525.1 .
AF334791 mRNA. Translation: AAK07651.1 .
U92897 mRNA. Translation: AAB53321.1 .
RefSeqi NP_001257891.1. NM_001270962.1.
NP_001257892.1. NM_001270963.1.
NP_113927.2. NM_031739.3. [Q62897-3 ]
UniGenei Rn.10540.
Rn.214215.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I2R X-ray 3.35 A/B/C/D/I/J/K/L 2-143 [» ]
ProteinModelPortali Q62897.
SMRi Q62897. Positions 3-140.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29245N.
IntActi Q62897. 1 interaction.
MINTi MINT-103596.

Chemistry

GuidetoPHARMACOLOGYi 554.

PTM databases

PhosphoSitei Q62897.

Proteomic databases

PaxDbi Q62897.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000019997 ; ENSRNOP00000019997 ; ENSRNOG00000014686 .
ENSRNOT00000051835 ; ENSRNOP00000049111 ; ENSRNOG00000014686 .
GeneIDi 65195.
KEGGi rno:65195.
UCSCi RGD:68394. rat. [Q62897-1 ]

Organism-specific databases

CTDi 3752.
RGDi 68394. Kcnd3.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00760000118846.
HOGENOMi HOG000231013.
HOVERGENi HBG106687.
InParanoidi Q62897.
KOi K04893.
PhylomeDBi Q62897.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

EvolutionaryTracei Q62897.
NextBioi 614128.
PROi Q62897.

Gene expression databases

ExpressionAtlasi Q62897. baseline and differential.
Genevestigatori Q62897.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004056. K_chnl_volt-dep_Kv4.3.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01518. KV43CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role of the Kv4.3 K+ channel in ventricular muscle. A molecular correlate for the transient outward current."
    Dixon J.E., Shi W., Wang H.-S., McDonald C., Yu H., Wymore R.S., Cohen I.S., McKinnon D.
    Circ. Res. 79:659-668(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
  2. "Cloning of a novel component of A-type K+ channels operating at subthreshold potentials with unique expression in heart and brain."
    Serodio P., Vega-Saenz de Miera E., Rudy B.
    J. Neurophysiol. 75:2174-2179(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain.
  3. "Cloning, expression and CNS distribution of Kv4.3, an A-type K+ channel alpha subunit."
    Tsaur M.-L., Chou C.-C., Shih Y.-H., Wang H.-L.
    FEBS Lett. 400:215-220(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Hippocampus.
  4. "Molecular cloning and tissue distribution of an alternatively spliced variant of an A-type K+ channel alpha-subunit, Kv4.3 in the rat."
    Ohya S., Tanaka M., Oku T., Asai Y., Watanabe M., Giles W.R., Imaizumi Y.
    FEBS Lett. 420:47-53(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Smooth muscle and Vas deferens.
  5. "Remodeling of Kv4.3 potassium channel gene expression under the control of sex hormones."
    Song M., Helguera G., Eghbali M., Zhu N., Zarei M.M., Olcese R., Toro L., Stefani E.
    J. Biol. Chem. 276:31883-31890(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Uterus.
  6. "Decreased expression of Kv4.2 and novel Kv4.3 K+ channel subunit mRNAs in ventricles of renovascular hypertensive rats."
    Takimoto K., Li D., Hershman K.M., Li P., Jackson E.K., Levitan E.S.
    Circ. Res. 81:533-539(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-606 (ISOFORM 1).
    Strain: Sprague-Dawley.
  7. Cited for: INTERACTION WITH KCNIP1; KCNIP2 AND KCNIP3.
  8. "Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels."
    Takimoto K., Yang E.-K., Conforti L.
    J. Biol. Chem. 277:26904-26911(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: INTERACTION WITH KCNIP4.
  10. Cited for: PHOSPHORYLATION AT SER-569 BY CAMK2D.
  11. "Kv4 potassium channels form a tripartite complex with the anchoring protein SAP97 and CaMKII in cardiac myocytes."
    El-Haou S., Balse E., Neyroud N., Dilanian G., Gavillet B., Abriel H., Coulombe A., Jeromin A., Hatem S.N.
    Circ. Res. 104:758-769(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  12. "Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer."
    Pioletti M., Findeisen F., Hura G.L., Minor D.L. Jr.
    Nat. Struct. Mol. Biol. 13:987-995(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 2-143 IN COMPLEX WITH KCNIP1.

Entry informationi

Entry nameiKCND3_RAT
AccessioniPrimary (citable) accession number: Q62897
Secondary accession number(s): O08723
, P70622, Q63286, Q99P42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: November 26, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3