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Protein

Potassium voltage-gated channel subfamily D member 3

Gene

Kcnd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi104ZincBy similarity1
Metal bindingi131ZincBy similarity1
Metal bindingi132ZincBy similarity1

GO - Molecular functioni

  • A-type (transient outward) potassium channel activity Source: RGD
  • ion channel activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • potassium channel activity Source: RGD
  • voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  • cellular response to BMP stimulus Source: RGD
  • potassium ion transport Source: RGD
  • protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Metal-binding, Potassium, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.
R-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily D member 3
Alternative name(s):
Voltage-gated potassium channel subunit Kv4.3
Gene namesi
Name:Kcnd3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi68394. Kcnd3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 181CytoplasmicSequence analysisAdd BLAST181
Transmembranei182 – 202Helical; Name=Segment S1Sequence analysisAdd BLAST21
Transmembranei222 – 242Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini243 – 256CytoplasmicSequence analysisAdd BLAST14
Transmembranei257 – 277Helical; Name=Segment S3Sequence analysisAdd BLAST21
Transmembranei287 – 307Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini308 – 320CytoplasmicSequence analysisAdd BLAST13
Transmembranei321 – 341Helical; Name=Segment S5Sequence analysisAdd BLAST21
Intramembranei360 – 380Pore-forming; Name=Segment H5Sequence analysisAdd BLAST21
Transmembranei382 – 402Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini403 – 655CytoplasmicSequence analysisAdd BLAST253

GO - Cellular componenti

  • caveola Source: UniProtKB
  • dendrite Source: RGD
  • integral component of plasma membrane Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • perinuclear endoplasmic reticulum Source: RGD
  • plasma membrane Source: RGD
  • sarcolemma Source: UniProtKB-SubCell
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi554.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540711 – 655Potassium voltage-gated channel subfamily D member 3Add BLAST655

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei153PhosphoserineCombined sources1
Modified residuei459PhosphothreonineBy similarity1
Modified residuei569Phosphoserine; by CaMK2D1 Publication1
Modified residuei585PhosphoserineBy similarity1

Post-translational modificationi

Regulated through phosphorylation at Ser-569 by CaMK2D.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62897.
PRIDEiQ62897.

PTM databases

iPTMnetiQ62897.
PhosphoSitePlusiQ62897.

Expressioni

Tissue specificityi

Highly expressed in brain, in particular in the retrosplenial cortex, medial habenula, anterior thalamus, hippocampus, cerebellum and lateral geniculate and superior colliculus. Highly expressed in heart atrium (at protein level) and throughout the ventricle wall, in lung and vas deferens.5 Publications

Gene expression databases

BgeeiENSRNOG00000014686.
ExpressionAtlasiQ62897. baseline and differential.

Interactioni

Subunit structurei

Homotetramer or heterotetramer with KCND1 and/or KCND2. Interacts with DLG1. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Interacts with KCNE1, KCNE2, SCN1B and KCNAB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi249307. 1 interactor.
DIPiDIP-29245N.
IntActiQ62897. 1 interactor.
MINTiMINT-103596.
STRINGi10116.ENSRNOP00000019997.

Structurei

Secondary structure

1655
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Helixi9 – 16Combined sources8
Beta strandi41 – 46Combined sources6
Beta strandi49 – 54Combined sources6
Helixi55 – 59Combined sources5
Turni65 – 67Combined sources3
Helixi70 – 74Combined sources5
Turni77 – 80Combined sources4
Beta strandi81 – 84Combined sources4
Helixi88 – 99Combined sources12
Helixi111 – 121Combined sources11
Turni130 – 132Combined sources3
Helixi133 – 138Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I2RX-ray3.35A/B/C/D/I/J/K/L2-143[»]
ProteinModelPortaliQ62897.
SMRiQ62897.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62897.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 20Interaction with KCNIP2By similarityAdd BLAST19
Regioni474 – 489Mediates dendritic targetingBy similarityAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi367 – 372Selectivity filterBy similarity6

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4390. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231013.
HOVERGENiHBG106687.
InParanoidiQ62897.
KOiK04893.
PhylomeDBiQ62897.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004056. K_chnl_volt-dep_Kv4.3.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type_N.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01518. KV43CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62897-1) [UniParc]FASTAAdd to basket
Also known as: Kv4.3 long form

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR
60 70 80 90 100
FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT
110 120 130 140 150
GKLHYPRYEC ISAYDDELAF YGILPEIIGD CCYEEYKDRK RENAERLMDD
160 170 180 190 200
NESENNQESM PSLSFRQTMW RAFENPHTST LALVFYYVTG FFIAVSVITN
210 220 230 240 250
VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE YLLRLFAAPS
260 270 280 290 300
RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF
310 320 330 340 350
SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK
360 370 380 390 400
FTSIPASFWY TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP
410 420 430 440 450
VIVSNFSRIY HQNQRADKRR AQKKARLARI RVAKTGSSNA YLHSKRNGLL
460 470 480 490 500
NEALELTGTP EEEHMGKTTS LIESQHHHLL HCLEKTTGLS YLVDDPLLSV
510 520 530 540 550
RTSTIKNHEF IDEQMFEQNC MESSMQNYPS TRSPSLSSHS GLTTTCCSRR
560 570 580 590 600
SKKTTHLPNS NLPATRLRSM QELSTIHIQG SEQPSLTTSR SSLNLKADDG
610 620 630 640 650
LRPNCKTSQI TTAIISIPTP PALTPEGESR PPPASPGPNT NIPSITSNVV

KVSVL
Length:655
Mass (Da):73,513
Last modified:November 7, 2003 - v2
Checksum:i26BC512BDE069C09
GO
Isoform 2 (identifier: Q62897-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     488-506: Missing.

Show »
Length:636
Mass (Da):71,454
Checksum:iA2D65B7C3FA5A58A
GO
Isoform 3 (identifier: Q62897-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     488-506: Missing.
     608-655: SQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSITSNVVKVSVL → QDQEQPRGRVVTCKQEEIITLCI

Show »
Length:611
Mass (Da):69,294
Checksum:i0D6042030E46F4C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124L → H in AAA80459 (PubMed:9001401).Curated1
Sequence conflicti404S → T in AAC52695 (PubMed:8734615).Curated1
Sequence conflicti404S → T in AAK07651 (PubMed:11427525).Curated1
Sequence conflicti569S → T in AAA80459 (PubMed:9001401).Curated1
Sequence conflicti631P → A in AAC52695 (PubMed:8734615).Curated1
Sequence conflicti654V → A in AAB18337 (PubMed:8831489).Curated1
Sequence conflicti654V → A in BAA24525 (PubMed:9450548).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008831488 – 506Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST19
Alternative sequenceiVSP_008832608 – 655SQITT…KVSVL → QDQEQPRGRVVTCKQEEIIT LCI in isoform 3. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75448 mRNA. Translation: AAB18337.1.
U42975 mRNA. Translation: AAC52695.1.
L48619 mRNA. Translation: AAA80459.1.
AB003587 mRNA. Translation: BAA24525.1.
AF334791 mRNA. Translation: AAK07651.1.
U92897 mRNA. Translation: AAB53321.1.
RefSeqiNP_001257891.1. NM_001270962.1.
NP_001257892.1. NM_001270963.1.
NP_113927.2. NM_031739.3. [Q62897-3]
UniGeneiRn.10540.
Rn.214215.

Genome annotation databases

EnsembliENSRNOT00000047827; ENSRNOP00000041746; ENSRNOG00000014686. [Q62897-3]
GeneIDi65195.
KEGGirno:65195.
UCSCiRGD:68394. rat. [Q62897-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75448 mRNA. Translation: AAB18337.1.
U42975 mRNA. Translation: AAC52695.1.
L48619 mRNA. Translation: AAA80459.1.
AB003587 mRNA. Translation: BAA24525.1.
AF334791 mRNA. Translation: AAK07651.1.
U92897 mRNA. Translation: AAB53321.1.
RefSeqiNP_001257891.1. NM_001270962.1.
NP_001257892.1. NM_001270963.1.
NP_113927.2. NM_031739.3. [Q62897-3]
UniGeneiRn.10540.
Rn.214215.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I2RX-ray3.35A/B/C/D/I/J/K/L2-143[»]
ProteinModelPortaliQ62897.
SMRiQ62897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249307. 1 interactor.
DIPiDIP-29245N.
IntActiQ62897. 1 interactor.
MINTiMINT-103596.
STRINGi10116.ENSRNOP00000019997.

Chemistry databases

GuidetoPHARMACOLOGYi554.

PTM databases

iPTMnetiQ62897.
PhosphoSitePlusiQ62897.

Proteomic databases

PaxDbiQ62897.
PRIDEiQ62897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000047827; ENSRNOP00000041746; ENSRNOG00000014686. [Q62897-3]
GeneIDi65195.
KEGGirno:65195.
UCSCiRGD:68394. rat. [Q62897-1]

Organism-specific databases

CTDi3752.
RGDi68394. Kcnd3.

Phylogenomic databases

eggNOGiKOG4390. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231013.
HOVERGENiHBG106687.
InParanoidiQ62897.
KOiK04893.
PhylomeDBiQ62897.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.
R-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

EvolutionaryTraceiQ62897.
PROiQ62897.

Gene expression databases

BgeeiENSRNOG00000014686.
ExpressionAtlasiQ62897. baseline and differential.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004056. K_chnl_volt-dep_Kv4.3.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type_N.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01518. KV43CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCND3_RAT
AccessioniPrimary (citable) accession number: Q62897
Secondary accession number(s): O08723
, P70622, Q63286, Q99P42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.