Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q62897

- KCND3_RAT

UniProt

Q62897 - KCND3_RAT

Protein

Potassium voltage-gated channel subfamily D member 3

Gene

Kcnd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi104 – 1041ZincBy similarity
    Metal bindingi131 – 1311ZincBy similarity
    Metal bindingi132 – 1321ZincBy similarity

    GO - Molecular functioni

    1. A-type (transient outward) potassium channel activity Source: RGD
    2. ion channel activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW
    4. potassium channel activity Source: RGD
    5. protein binding Source: RGD
    6. voltage-gated potassium channel activity Source: RGD

    GO - Biological processi

    1. membrane repolarization Source: Ensembl
    2. potassium ion export Source: Ensembl
    3. potassium ion transport Source: RGD
    4. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Metal-binding, Potassium, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily D member 3
    Alternative name(s):
    Voltage-gated potassium channel subunit Kv4.3
    Gene namesi
    Name:Kcnd3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi68394. Kcnd3.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cell membranesarcolemma. Cell projectiondendrite By similarity
    Note: Interaction with palmitoylated KCNIP2 and KCNIP3 enhances cell surface expression.

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. integral component of plasma membrane Source: RGD
    3. neuronal cell body Source: RGD
    4. neuron projection Source: RGD
    5. perinuclear endoplasmic reticulum Source: RGD
    6. plasma membrane Source: RGD
    7. sarcolemma Source: UniProtKB-SubCell
    8. voltage-gated potassium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 655655Potassium voltage-gated channel subfamily D member 3PRO_0000054071Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei569 – 5691Phosphoserine; by CaMK2D1 Publication

    Post-translational modificationi

    Regulated through phosphorylation at Ser-569 by CaMK2D.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ62897.

    PTM databases

    PhosphoSiteiQ62897.

    Expressioni

    Tissue specificityi

    Highly expressed in brain, in particular in the retrosplenial cortex, medial habenula, anterior thalamus, hippocampus, cerebellum and lateral geniculate and superior colliculus. Highly expressed in heart atrium (at protein level) and throughout the ventricle wall, in lung and vas deferens.5 Publications

    Gene expression databases

    GenevestigatoriQ62897.

    Interactioni

    Subunit structurei

    Homotetramer or heterotetramer with KCND1 and/or KCND2. Interacts with DLG1. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Interacts with KCNE1, KCNE2, SCN1B and KCNAB1 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-29245N.
    IntActiQ62897. 1 interaction.
    MINTiMINT-103596.

    Structurei

    Secondary structure

    1
    655
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni6 – 83
    Helixi9 – 168
    Beta strandi41 – 466
    Beta strandi49 – 546
    Helixi55 – 595
    Turni65 – 673
    Helixi70 – 745
    Turni77 – 804
    Beta strandi81 – 844
    Helixi88 – 9912
    Helixi111 – 12111
    Turni130 – 1323
    Helixi133 – 1386

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2I2RX-ray3.35A/B/C/D/I/J/K/L2-143[»]
    ProteinModelPortaliQ62897.
    SMRiQ62897. Positions 3-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62897.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 181181CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini243 – 25614CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 32013CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini403 – 655253CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei360 – 38021Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei182 – 20221Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei222 – 24221Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei257 – 27721Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei287 – 30721Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei321 – 34121Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei382 – 40221Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 2019Interaction with KCNIP2By similarityAdd
    BLAST
    Regioni474 – 48916Mediates dendritic targetingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi367 – 3726Selectivity filterBy similarity

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115211.
    HOGENOMiHOG000231013.
    HOVERGENiHBG106687.
    InParanoidiQ62897.
    KOiK04893.
    PhylomeDBiQ62897.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003975. K_chnl_volt-dep_Kv4.
    IPR004056. K_chnl_volt-dep_Kv4.3.
    IPR024587. K_chnl_volt-dep_Kv4_C.
    IPR021645. Shal-type.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF11879. DUF3399. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF11601. Shal-type. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01518. KV43CHANNEL.
    PR01491. KVCHANNEL.
    PR01497. SHALCHANNEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q62897-1) [UniParc]FASTAAdd to Basket

    Also known as: Kv4.3 long form

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR    50
    FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT 100
    GKLHYPRYEC ISAYDDELAF YGILPEIIGD CCYEEYKDRK RENAERLMDD 150
    NESENNQESM PSLSFRQTMW RAFENPHTST LALVFYYVTG FFIAVSVITN 200
    VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE YLLRLFAAPS 250
    RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF 300
    SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK 350
    FTSIPASFWY TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP 400
    VIVSNFSRIY HQNQRADKRR AQKKARLARI RVAKTGSSNA YLHSKRNGLL 450
    NEALELTGTP EEEHMGKTTS LIESQHHHLL HCLEKTTGLS YLVDDPLLSV 500
    RTSTIKNHEF IDEQMFEQNC MESSMQNYPS TRSPSLSSHS GLTTTCCSRR 550
    SKKTTHLPNS NLPATRLRSM QELSTIHIQG SEQPSLTTSR SSLNLKADDG 600
    LRPNCKTSQI TTAIISIPTP PALTPEGESR PPPASPGPNT NIPSITSNVV 650
    KVSVL 655
    Length:655
    Mass (Da):73,513
    Last modified:November 7, 2003 - v2
    Checksum:i26BC512BDE069C09
    GO
    Isoform 2 (identifier: Q62897-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         488-506: Missing.

    Show »
    Length:636
    Mass (Da):71,454
    Checksum:iA2D65B7C3FA5A58A
    GO
    Isoform 3 (identifier: Q62897-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         488-506: Missing.
         608-655: SQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSITSNVVKVSVL → QDQEQPRGRVVTCKQEEIITLCI

    Show »
    Length:611
    Mass (Da):69,294
    Checksum:i0D6042030E46F4C1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti124 – 1241L → H in AAA80459. (PubMed:9001401)Curated
    Sequence conflicti404 – 4041S → T in AAC52695. (PubMed:8734615)Curated
    Sequence conflicti404 – 4041S → T in AAK07651. (PubMed:11427525)Curated
    Sequence conflicti569 – 5691S → T in AAA80459. (PubMed:9001401)Curated
    Sequence conflicti631 – 6311P → A in AAC52695. (PubMed:8734615)Curated
    Sequence conflicti654 – 6541V → A in AAB18337. (PubMed:8831489)Curated
    Sequence conflicti654 – 6541V → A in BAA24525. (PubMed:9450548)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei488 – 50619Missing in isoform 2 and isoform 3. 3 PublicationsVSP_008831Add
    BLAST
    Alternative sequencei608 – 65548SQITT…KVSVL → QDQEQPRGRVVTCKQEEIIT LCI in isoform 3. 1 PublicationVSP_008832Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U75448 mRNA. Translation: AAB18337.1.
    U42975 mRNA. Translation: AAC52695.1.
    L48619 mRNA. Translation: AAA80459.1.
    AB003587 mRNA. Translation: BAA24525.1.
    AF334791 mRNA. Translation: AAK07651.1.
    U92897 mRNA. Translation: AAB53321.1.
    RefSeqiNP_001257891.1. NM_001270962.1.
    NP_001257892.1. NM_001270963.1.
    NP_113927.2. NM_031739.3. [Q62897-3]
    UniGeneiRn.10540.
    Rn.214215.

    Genome annotation databases

    EnsembliENSRNOT00000019997; ENSRNOP00000019997; ENSRNOG00000014686.
    ENSRNOT00000051835; ENSRNOP00000049111; ENSRNOG00000014686.
    GeneIDi65195.
    KEGGirno:65195.
    UCSCiRGD:68394. rat. [Q62897-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U75448 mRNA. Translation: AAB18337.1 .
    U42975 mRNA. Translation: AAC52695.1 .
    L48619 mRNA. Translation: AAA80459.1 .
    AB003587 mRNA. Translation: BAA24525.1 .
    AF334791 mRNA. Translation: AAK07651.1 .
    U92897 mRNA. Translation: AAB53321.1 .
    RefSeqi NP_001257891.1. NM_001270962.1.
    NP_001257892.1. NM_001270963.1.
    NP_113927.2. NM_031739.3. [Q62897-3 ]
    UniGenei Rn.10540.
    Rn.214215.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2I2R X-ray 3.35 A/B/C/D/I/J/K/L 2-143 [» ]
    ProteinModelPortali Q62897.
    SMRi Q62897. Positions 3-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29245N.
    IntActi Q62897. 1 interaction.
    MINTi MINT-103596.

    Chemistry

    GuidetoPHARMACOLOGYi 554.

    PTM databases

    PhosphoSitei Q62897.

    Proteomic databases

    PaxDbi Q62897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019997 ; ENSRNOP00000019997 ; ENSRNOG00000014686 .
    ENSRNOT00000051835 ; ENSRNOP00000049111 ; ENSRNOG00000014686 .
    GeneIDi 65195.
    KEGGi rno:65195.
    UCSCi RGD:68394. rat. [Q62897-1 ]

    Organism-specific databases

    CTDi 3752.
    RGDi 68394. Kcnd3.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115211.
    HOGENOMi HOG000231013.
    HOVERGENi HBG106687.
    InParanoidi Q62897.
    KOi K04893.
    PhylomeDBi Q62897.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    EvolutionaryTracei Q62897.
    NextBioi 614128.
    PROi Q62897.

    Gene expression databases

    Genevestigatori Q62897.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003975. K_chnl_volt-dep_Kv4.
    IPR004056. K_chnl_volt-dep_Kv4.3.
    IPR024587. K_chnl_volt-dep_Kv4_C.
    IPR021645. Shal-type.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF11879. DUF3399. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF11601. Shal-type. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01518. KV43CHANNEL.
    PR01491. KVCHANNEL.
    PR01497. SHALCHANNEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Role of the Kv4.3 K+ channel in ventricular muscle. A molecular correlate for the transient outward current."
      Dixon J.E., Shi W., Wang H.-S., McDonald C., Yu H., Wymore R.S., Cohen I.S., McKinnon D.
      Circ. Res. 79:659-668(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    2. "Cloning of a novel component of A-type K+ channels operating at subthreshold potentials with unique expression in heart and brain."
      Serodio P., Vega-Saenz de Miera E., Rudy B.
      J. Neurophysiol. 75:2174-2179(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Brain.
    3. "Cloning, expression and CNS distribution of Kv4.3, an A-type K+ channel alpha subunit."
      Tsaur M.-L., Chou C.-C., Shih Y.-H., Wang H.-L.
      FEBS Lett. 400:215-220(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Hippocampus.
    4. "Molecular cloning and tissue distribution of an alternatively spliced variant of an A-type K+ channel alpha-subunit, Kv4.3 in the rat."
      Ohya S., Tanaka M., Oku T., Asai Y., Watanabe M., Giles W.R., Imaizumi Y.
      FEBS Lett. 420:47-53(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Smooth muscle and Vas deferens.
    5. "Remodeling of Kv4.3 potassium channel gene expression under the control of sex hormones."
      Song M., Helguera G., Eghbali M., Zhu N., Zarei M.M., Olcese R., Toro L., Stefani E.
      J. Biol. Chem. 276:31883-31890(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Sprague-Dawley.
      Tissue: Uterus.
    6. "Decreased expression of Kv4.2 and novel Kv4.3 K+ channel subunit mRNAs in ventricles of renovascular hypertensive rats."
      Takimoto K., Li D., Hershman K.M., Li P., Jackson E.K., Levitan E.S.
      Circ. Res. 81:533-539(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-606 (ISOFORM 1).
      Strain: Sprague-Dawley.
    7. Cited for: INTERACTION WITH KCNIP1; KCNIP2 AND KCNIP3.
    8. "Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels."
      Takimoto K., Yang E.-K., Conforti L.
      J. Biol. Chem. 277:26904-26911(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. Cited for: INTERACTION WITH KCNIP4.
    10. Cited for: PHOSPHORYLATION AT SER-569 BY CAMK2D.
    11. "Kv4 potassium channels form a tripartite complex with the anchoring protein SAP97 and CaMKII in cardiac myocytes."
      El-Haou S., Balse E., Neyroud N., Dilanian G., Gavillet B., Abriel H., Coulombe A., Jeromin A., Hatem S.N.
      Circ. Res. 104:758-769(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    12. "Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer."
      Pioletti M., Findeisen F., Hura G.L., Minor D.L. Jr.
      Nat. Struct. Mol. Biol. 13:987-995(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 2-143 IN COMPLEX WITH KCNIP1.

    Entry informationi

    Entry nameiKCND3_RAT
    AccessioniPrimary (citable) accession number: Q62897
    Secondary accession number(s): O08723
    , P70622, Q63286, Q99P42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3