Reviewed, UniProtKB/Swiss-Prot Q62893 (AMHR2_RAT)

Names and origin

Protein names

Recommended name:
    Anti-Muellerian hormone type-2 receptor
    EC=2.7.11.30
Alternative name(s):
    Anti-Muellerian hormone type II receptor
      Short name=AMH type II receptor
    MIS type II receptor
    MISRII
      Short name=MRII
    C14

Gene names

Name:

Amhr2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Developmental stage	Expressed in the mesenchymal cells surrounding the Muellerian duct at embryonic days 14, 15, and 16 and in tubular and follicular structures of the fetal gonads.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	sex differentiation Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hormone binding Inferred from sequence or structural similarity. Source: UniProtKB receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 557	540	Anti-Muellerian hormone type-2 receptor
Regions
	Topological domain	18 – 144	127	Extracellular Potential
	Transmembrane	145 – 165	21	Potential
	Topological domain	166 – 557	392	Cytoplasmic Potential
	Domain	201 – 511	311	Protein kinase
	Nucleotide binding	207 – 215	9	ATP By similarity
Sites
	Active site	331	1	Proton acceptor By similarity
	Binding site	228	1	ATP By similarity
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	527	1	C → Y in CAA50731. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q62893-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8EDEE9C0C32EBDD5	FASTA	557	59,749
	10 20 30 40 50 60 MLGTLGLWTL LPAAAQVSPN RRTCVFFEAP GVRGSTKTLG EVVDAGPGPP KGIRCLYSHC 70 80 90 100 110 120 CFGIWNLTHG RAQVEMQGCL DSDEPGCESL HCDPVPRAHP SPSSTLFTCS CGTDFCNANY 130 140 150 160 170 180 SHLPPSGNRG APGPQEPQAT PGGPIWMAQL LLGVFLVLLL SIIILALLQR KACRVQGGSD 190 200 210 220 230 240 PEPEPGSGGD CSEELPELAE LRFSQVIQEG GHAVVWAGRL QGEMVAIKAF PPRAVAQFRA 250 260 270 280 290 300 ERAVYQLLGL QHNHIVRFIT AGQGGPGPLP SGPLLVLELY PKGSLCHYLT QYTSDWGSSL 310 320 330 340 350 360 RMALSLAEGL AFLHGERWQD GQYKPGIAHR DLSSQNVLIR EDRSCAIGDL GLALVLPGLA 370 380 390 400 410 420 QPPALAPTQP RGPAAILEAG TQRYMAPELL DKTLDLQDWG TALQRADVYS LALLLWEILS 430 440 450 460 470 480 RCSDLRPDHR PPPFQLAYEA ELGSNPSACE LWALAVAERK RPNIPSSWSC SATDPRGLRE 490 500 510 520 530 540 LLEDCWDADP EARLTAECVQ QRLAALAYPQ VASSFPESCP QGCPENCPAA PASAAFPCRP 550 QQSSCLLSVQ QGSGSKS

References

[1]	"Developmental expression of a candidate Muellerian inhibiting substance type II receptor." Teixeira J., He W.W., Shah P.C., Morikawa N., Lee M.M., Catlin E.A., Hudson P.L., Wing J., Maclaughlin D.T., Donahoe P.K. Endocrinology 137:160-165(1996) [PubMed: 8536608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A novel member of the transmembrane serine/threonine kinase receptor family is specifically expressed in the gonads and in mesenchymal cells adjacent to the Muellerian duct." Baarends W.M., Van Helmond M.J.L., Post M., Van der Schoot P.J.C.M., Hoogerbrugge J.W., de Winter J.P., Uilenbroek J.T.J., Karels B., Wilming L.G., Meijers J.H.C., Themmem A.P.N., Grootegoed A.J. Development 120:189-197(1994) [PubMed: 8119126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"Transcriptional regulation of the rat Muellerian inhibiting substance type II receptor in rodent Leydig cells." Teixeira J., Kehas D.J., Antun R., Donahoe P.K. Proc. Natl. Acad. Sci. U.S.A. 96:13831-13838(1999) [PubMed: 10570158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U42427 mRNA. Translation: AAC52343.1. X71916 mRNA. Translation: CAA50731.1. AF092445 Genomic DNA. Translation: AAC64138.1.
PIR	S41627.
RefSeq	NP_112260.1.
UniGene	Rn.10165
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000014850. Rattus norvegicus. [Contig view]
GeneID	29530.
KEGG	rno:29530.
Organism-specific databases
RGD	70964. Amhr2.
Phylogenomic databases
HOVERGEN	Q62893.
Gene expression databases
ArrayExpress	Q62893.
GermOnline	ENSRNOG00000014850. Rattus norvegicus.
Family and domain databases
InterPro	IPR017193. Anti-muellerian_hrmn_rcpt_II. IPR015771. Anti-muellerian_hrmn_rcpt_II_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
PANTHER	PTHR23255:SF4. MIS_II_R. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037392. AMHRII. 1 hit.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AMHR2_RAT

Accession

Primary (citable) accession number: Q62893
Secondary accession number(s): Q63045, Q9R0A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	September 2, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)