Neuroligin-3 precursor - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Neuroligin-3

Alternative name(s):
Gliotactin homolog

Gene names

Name:

Nlgn3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	848 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Ref.5
Subunit structure	Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) By similarity. Homodimer, and heterodimer with NLGN1 and NLGN2 By similarity. Ref.1 Ref.3 Ref.7
Subcellular location	Cell membrane; Single-pass type I membrane protein. Cell junction › synapse. Note: Detected at both glutamatergic and GABAergic synapses. Ref.3 Ref.5 Ref.8
Tissue specificity	Detected in brain and on hippocampus neurons, especially at excitatory synapses. Detected in retina (at protein level). Expressed in brain, spinal chord and dorsal root ganglion. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.8
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon extension Inferred from mutant phenotype PubMed 19406211. Source: BHF-UCL neuron cell-cell adhesion Inferred from direct assay PubMed 15797875. Source: BHF-UCL positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of excitatory postsynaptic membrane potential Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of synapse assembly Inferred from direct assay PubMed 15797875. Source: BHF-UCL positive regulation of synaptic transmission, glutamatergic Inferred from sequence or structural similarity. Source: BHF-UCL postsynaptic membrane assembly Inferred from sequence or structural similarity. Source: BHF-UCL presynaptic membrane assembly Inferred from direct assay PubMed 15797875. Source: BHF-UCL receptor-mediated endocytosis Inferred from direct assay PubMed 15797875. Source: BHF-UCL regulation of N-methyl-D-aspartate selective glutamate receptor activity Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL regulation of dendritic spine morphogenesis Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL regulation of inhibitory postsynaptic membrane potential Inferred from direct assay PubMed 19406211. Source: BHF-UCL regulation of long-term synaptic potentiation Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL regulation of terminal button organization Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL rhythmic synaptic transmission Inferred from mutant phenotype PubMed 19406211. Source: BHF-UCL social behavior Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cell surface Inferred from direct assay PubMed 15797875. Source: BHF-UCL endocytic vesicle Inferred from direct assay PubMed 15797875. Source: BHF-UCL excitatory synapse Inferred from direct assay Ref.8. Source: BHF-UCL inhibitory synapse Inferred by curator PubMed 19406211. Source: BHF-UCL integral to plasma membrane Inferred from direct assay PubMed 15797875. Source: BHF-UCL
Molecular_function	neurexin family protein binding Inferred from Biological aspect of Ancestor. Source: RefGenome receptor activity Inferred from direct assay PubMed 15797875. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q62889-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62889-2) The sequence of this isoform differs from the canonical sequence as follows: 153-172: Missing. 173-192: Missing.
Note: No experimental confirmation available.

Isoform 3 (identifier: Q62889-3) The sequence of this isoform differs from the canonical sequence as follows: 173-192: Missing.

Isoform 4 (identifier: Q62889-4) The sequence of this isoform differs from the canonical sequence as follows: 153-172: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 37

37

Potential

Chain

38 – 848

811

Neuroligin-3

PRO_0000008647

Regions

Topological domain

38 – 709

672

Extracellular Potential

Transmembrane

710 – 730

21

Helical; Potential

Topological domain

731 – 848

118

Cytoplasmic Potential

Amino acid modifications

Modified residue

792

1

Phosphotyrosine By similarity

Glycosylation

98

1

N-linked (GlcNAc...) Potential

Glycosylation

545

1

N-linked (GlcNAc...) Potential

Disulfide bond

106 ↔ 141

By similarity

Disulfide bond

340 ↔ 351

By similarity

Disulfide bond

510 ↔ 544

By similarity

Natural variations

Alternative sequence

153 – 172

20

Missing in isoform 2 and isoform 4.

VSP_007535

Alternative sequence

173 – 192

20

Missing in isoform 2 and isoform 3.

VSP_007536

Experimental info

Mutagenesis

451

1

R → C: Impaired cell surface expression, and reduced interaction with NRXN1. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7520653B3253E750 Show »	FASTA	848	93,888
10 20 30 40 50 60 MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK LRGARVPLPS 70 80 90 100 110 120 EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT HFPPVCPQNI HTAVPEVMLP 130 140 150 160 170 180 VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDVKRISKEC ARKPNKKICR KGGSGAKKQG 190 200 210 220 230 240 EDLADNDGDE DEDIRDSGAK PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG 250 260 270 280 290 300 VLGFLSTGDQ AAKGNYGLLD QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS 310 320 330 340 350 360 HHSEGLFQRA IIQSGSALSS WAVNYQPVKY TSLLADKVGC NVLDTVDMVD CLRQKSAKEL 370 380 390 400 410 420 VEQDIQPARY HVAFGPVIDG DVIPDDPEIL MEQGEFLNYD IMLGVNQGEG LKFVEGVVDP 430 440 450 460 470 480 EDGVSGTDFD YSVSNFVDNL YGYPEGKDTL RETIKFMYTD WADRDNPETR RKTLVALFTD 490 500 510 520 530 540 HQWVEPSVVT ADLHARYGSP TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD 550 560 570 580 590 600 LFPCNFSKND VMLSAVVMTY WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD 610 620 630 640 650 660 QLYLHIGLKP RVRDHYRATK VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP DTTHSSHITR 670 680 690 700 710 720 RPNGKTWSTK RPAISPAYSN ENAPGSWNGD QDAGPLLVEN PRDYSTELSV TIAVGASLLF 730 740 750 760 770 780 LNVLAFAALY YRKDKRRQEP LRQPSPQRGT GAPELGTAPE EELAALQLGP THHECEAGPP 790 800 810 820 830 840 HDTLRLTALP DYTLTLRRSP DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP NSHSTTRV « Hide
Isoform 2 [UniParc]. Checksum: C2B71B50BEB9B541 Show »	FASTA	808	89,531
10 20 30 40 50 60 MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK LRGARVPLPS 70 80 90 100 110 120 EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT HFPPVCPQNI HTAVPEVMLP 130 140 150 160 170 180 VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDDIRDSGAK PVMVYIHGGS YMEGTGNMID 190 200 210 220 230 240 GSVLASYGNV IVITLNYRVG VLGFLSTGDQ AAKGNYGLLD QIQALRWVSE NIAFFGGDPR 250 260 270 280 290 300 RITVFGSGIG ASCVSLLTLS HHSEGLFQRA IIQSGSALSS WAVNYQPVKY TSLLADKVGC 310 320 330 340 350 360 NVLDTVDMVD CLRQKSAKEL VEQDIQPARY HVAFGPVIDG DVIPDDPEIL MEQGEFLNYD 370 380 390 400 410 420 IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL YGYPEGKDTL RETIKFMYTD 430 440 450 460 470 480 WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP TYFYAFYHHC QSLMKPAWSD 490 500 510 520 530 540 AAHGDEVPYV FGVPMVGPTD LFPCNFSKND VMLSAVVMTY WTNFAKTGDP NKPVPQDTKF 550 560 570 580 590 600 IHTKANRFEE VAWSKYNPRD QLYLHIGLKP RVRDHYRATK VAFWKHLVPH LYNLHDMFHY 610 620 630 640 650 660 TSTTTKVPPP DTTHSSHITR RPNGKTWSTK RPAISPAYSN ENAPGSWNGD QDAGPLLVEN 670 680 690 700 710 720 PRDYSTELSV TIAVGASLLF LNVLAFAALY YRKDKRRQEP LRQPSPQRGT GAPELGTAPE 730 740 750 760 770 780 EELAALQLGP THHECEAGPP HDTLRLTALP DYTLTLRRSP DDIPLMTPNT ITMIPNSLVG 790 800 LQTLHPYNTF AAGFNSTGLP NSHSTTRV « Hide
Isoform 3 [UniParc]. Checksum: 3CD9FC2E5B84472C Show »	FASTA	828	91,856
10 20 30 40 50 60 MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK LRGARVPLPS 70 80 90 100 110 120 EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT HFPPVCPQNI HTAVPEVMLP 130 140 150 160 170 180 VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDVKRISKEC ARKPNKKICR KGDIRDSGAK 190 200 210 220 230 240 PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG VLGFLSTGDQ AAKGNYGLLD 250 260 270 280 290 300 QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS HHSEGLFQRA IIQSGSALSS 310 320 330 340 350 360 WAVNYQPVKY TSLLADKVGC NVLDTVDMVD CLRQKSAKEL VEQDIQPARY HVAFGPVIDG 370 380 390 400 410 420 DVIPDDPEIL MEQGEFLNYD IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL 430 440 450 460 470 480 YGYPEGKDTL RETIKFMYTD WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP 490 500 510 520 530 540 TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD LFPCNFSKND VMLSAVVMTY 550 560 570 580 590 600 WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD QLYLHIGLKP RVRDHYRATK 610 620 630 640 650 660 VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP DTTHSSHITR RPNGKTWSTK RPAISPAYSN 670 680 690 700 710 720 ENAPGSWNGD QDAGPLLVEN PRDYSTELSV TIAVGASLLF LNVLAFAALY YRKDKRRQEP 730 740 750 760 770 780 LRQPSPQRGT GAPELGTAPE EELAALQLGP THHECEAGPP HDTLRLTALP DYTLTLRRSP 790 800 810 820 DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP NSHSTTRV « Hide
Isoform 4 [UniParc]. Checksum: D612DF45CF916C8B Show »	FASTA	828	91,563
10 20 30 40 50 60 MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK LRGARVPLPS 70 80 90 100 110 120 EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT HFPPVCPQNI HTAVPEVMLP 130 140 150 160 170 180 VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDGSGAKKQG EDLADNDGDE DEDIRDSGAK 190 200 210 220 230 240 PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG VLGFLSTGDQ AAKGNYGLLD 250 260 270 280 290 300 QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS HHSEGLFQRA IIQSGSALSS 310 320 330 340 350 360 WAVNYQPVKY TSLLADKVGC NVLDTVDMVD CLRQKSAKEL VEQDIQPARY HVAFGPVIDG 370 380 390 400 410 420 DVIPDDPEIL MEQGEFLNYD IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL 430 440 450 460 470 480 YGYPEGKDTL RETIKFMYTD WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP 490 500 510 520 530 540 TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD LFPCNFSKND VMLSAVVMTY 550 560 570 580 590 600 WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD QLYLHIGLKP RVRDHYRATK 610 620 630 640 650 660 VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP DTTHSSHITR RPNGKTWSTK RPAISPAYSN 670 680 690 700 710 720 ENAPGSWNGD QDAGPLLVEN PRDYSTELSV TIAVGASLLF LNVLAFAALY YRKDKRRQEP 730 740 750 760 770 780 LRQPSPQRGT GAPELGTAPE EELAALQLGP THHECEAGPP HDTLRLTALP DYTLTLRRSP 790 800 810 820 DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP NSHSTTRV « Hide

References

[1]	"Structures, alternative splicing, and neurexin binding of multiple neuroligins." Ichtchenko K., Nguyen T., Suedhof T.C. J. Biol. Chem. 271:2676-2682(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), BLOCKAGE OF N-TERMINUS, TISSUE SPECIFICITY, INTERACTION WITH NEUREXIN 1-BETA; NEUREXIN 2-BETA AND NEUREXIN 3-BETA. Tissue: Forebrain.
[2]	"Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia." Gilbert M., Smith J., Roskams A.J., Auld V.J. Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[3]	"The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing." Comoletti D., De Jaco A., Jennings L.L., Flynn R.E., Gaietta G., Tsigelny I., Ellisman M.H., Taylor P. J. Neurosci. 24:4889-4893(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ARG-451, SUBCELLULAR LOCATION, INTERACTION WITH NRXN1, MASS SPECTROMETRY.
[4]	"Neuroligins determine synapse maturation and function." Varoqueaux F., Aramuni G., Rawson R.L., Mohrmann R., Missler M., Gottmann K., Zhang W., Sudhof T.C., Brose N. Neuron 51:741-754(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses." Budreck E.C., Scheiffele P. Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion." Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D. Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[7]	"Splice form dependence of beta-neurexin/neuroligin binding interactions." Koehnke J., Katsamba P.S., Ahlsen G., Bahna F., Vendome J., Honig B., Shapiro L., Jin X. Neuron 67:61-74(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NRXN1.
[8]	"Postsynaptic scaffolding molecules modulate the localization of neuroligins." Levinson J.N., Li R., Kang R., Moukhles H., El-Husseini A., Bamji S.X. Neuroscience 165:782-793(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41663 mRNA. Translation: AAA97871.1.
IPI	IPI00209309. IPI00325803. IPI00325804. IPI00325805.
RefSeq	NP_599163.2. NM_134336.2.
UniGene	Rn.226139.
3D structure databases
ProteinModelPortal	Q62889.
SMR	Q62889. Positions 39-631.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1561818.
STRING	10116.ENSRNOP00000005077.
Protein family/group databases
MEROPS	S09.987.
PTM databases
PhosphoSite	Q62889.
Proteomic databases
PaxDb	Q62889.
PRIDE	Q62889.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	171297.
KEGG	rno:171297.
UCSC	RGD:621119. rat.
Organism-specific databases
CTD	54413.
RGD	621119. Nlgn3.
Phylogenomic databases
eggNOG	COG2272.
HOGENOM	HOG000231424.
HOVERGEN	HBG008839.
InParanoid	Q62889.
KO	K07378.
OrthoDB	EOG4CZBF6.
Gene expression databases
ArrayExpress	Q62889.
Genevestigator	Q62889.
GermOnline	ENSRNOG00000003812. Rattus norvegicus.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019819. Carboxylesterase_B_CS. IPR000460. Neuroligin. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR01090. NEUROLIGIN.
PROSITE	PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622053.

Entry information

Entry name

NLGN3_RAT

Accession

Primary (citable) accession number: Q62889

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents