Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuroligin-3

Gene

Nlgn3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.1 Publication

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: GO_Central
  • cell adhesion molecule binding Source: BHF-UCL
  • neurexin family protein binding Source: BHF-UCL
  • receptor activity Source: BHF-UCL

GO - Biological processi

  • axon extension Source: BHF-UCL
  • metabolic process Source: GOC
  • neuron cell-cell adhesion Source: BHF-UCL
  • positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  • positive regulation of dendritic spine development Source: RGD
  • positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  • positive regulation of inhibitory postsynaptic membrane potential Source: RGD
  • positive regulation of protein localization to synapse Source: RGD
  • positive regulation of synapse assembly Source: BHF-UCL
  • positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  • postsynaptic membrane assembly Source: BHF-UCL
  • presynaptic membrane assembly Source: BHF-UCL
  • receptor-mediated endocytosis Source: BHF-UCL
  • regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  • regulation of dendritic spine morphogenesis Source: BHF-UCL
  • regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  • regulation of inhibitory postsynaptic membrane potential Source: BHF-UCL
  • regulation of long-term synaptic potentiation Source: BHF-UCL
  • regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
  • regulation of respiratory gaseous exchange by neurological system process Source: BHF-UCL
  • regulation of synaptic transmission Source: BHF-UCL
  • regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  • regulation of terminal button organization Source: BHF-UCL
  • rhythmic synaptic transmission Source: BHF-UCL
  • social behavior Source: BHF-UCL
  • synapse assembly Source: BHF-UCL
  • synapse organization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Protein family/group databases

MEROPSiS09.987.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroligin-3
Alternative name(s):
Gliotactin homolog
Gene namesi
Name:Nlgn3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621119. Nlgn3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini38 – 709672ExtracellularSequence AnalysisAdd
BLAST
Transmembranei710 – 73021HelicalSequence AnalysisAdd
BLAST
Topological domaini731 – 848118CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell surface Source: BHF-UCL
  • endocytic vesicle Source: BHF-UCL
  • excitatory synapse Source: BHF-UCL
  • inhibitory synapse Source: BHF-UCL
  • integral component of plasma membrane Source: BHF-UCL
  • neuronal cell body Source: RGD
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi451 – 4511R → C: Impaired cell surface expression, and reduced interaction with NRXN1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence AnalysisAdd
BLAST
Chaini38 – 848811Neuroligin-3PRO_0000008647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi106 ↔ 141By similarity
Disulfide bondi340 ↔ 351By similarity
Disulfide bondi510 ↔ 544By similarity
Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis
Modified residuei792 – 7921PhosphotyrosineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62889.
PRIDEiQ62889.

PTM databases

PhosphoSiteiQ62889.

Expressioni

Tissue specificityi

Detected in brain and on hippocampus neurons, especially at excitatory synapses. Detected in retina (at protein level). Expressed in brain, spinal chord and dorsal root ganglion.6 Publications

Gene expression databases

GenevestigatoriQ62889.

Interactioni

Subunit structurei

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) (By similarity). Homodimer, and heterodimer with NLGN1 and NLGN2 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1561818.
STRINGi10116.ENSRNOP00000005077.

Structurei

3D structure databases

ProteinModelPortaliQ62889.
SMRiQ62889. Positions 39-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ62889.
KOiK07378.
PhylomeDBiQ62889.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030024. NLGN3.
[Graphical view]
PANTHERiPTHR11559:SF145. PTHR11559:SF145. 1 hit.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62889-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK
60 70 80 90 100
LRGARVPLPS EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT
110 120 130 140 150
HFPPVCPQNI HTAVPEVMLP VWFTANLDIV ATYIQEPNED CLYLNVYVPT
160 170 180 190 200
EDVKRISKEC ARKPNKKICR KGGSGAKKQG EDLADNDGDE DEDIRDSGAK
210 220 230 240 250
PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG VLGFLSTGDQ
260 270 280 290 300
AAKGNYGLLD QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS
310 320 330 340 350
HHSEGLFQRA IIQSGSALSS WAVNYQPVKY TSLLADKVGC NVLDTVDMVD
360 370 380 390 400
CLRQKSAKEL VEQDIQPARY HVAFGPVIDG DVIPDDPEIL MEQGEFLNYD
410 420 430 440 450
IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL YGYPEGKDTL
460 470 480 490 500
RETIKFMYTD WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP
510 520 530 540 550
TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD LFPCNFSKND
560 570 580 590 600
VMLSAVVMTY WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD
610 620 630 640 650
QLYLHIGLKP RVRDHYRATK VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP
660 670 680 690 700
DTTHSSHITR RPNGKTWSTK RPAISPAYSN ENAPGSWNGD QDAGPLLVEN
710 720 730 740 750
PRDYSTELSV TIAVGASLLF LNVLAFAALY YRKDKRRQEP LRQPSPQRGT
760 770 780 790 800
GAPELGTAPE EELAALQLGP THHECEAGPP HDTLRLTALP DYTLTLRRSP
810 820 830 840
DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP NSHSTTRV
Length:848
Mass (Da):93,888
Last modified:November 1, 1996 - v1
Checksum:i7520653B3253E750
GO
Isoform 2 (identifier: Q62889-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-172: Missing.
     173-192: Missing.

Note: No experimental confirmation available.

Show »
Length:808
Mass (Da):89,531
Checksum:iC2B71B50BEB9B541
GO
Isoform 3 (identifier: Q62889-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-192: Missing.

Show »
Length:828
Mass (Da):91,856
Checksum:i3CD9FC2E5B84472C
GO
Isoform 4 (identifier: Q62889-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-172: Missing.

Show »
Length:828
Mass (Da):91,563
Checksum:iD612DF45CF916C8B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei153 – 17220Missing in isoform 2 and isoform 4. 1 PublicationVSP_007535Add
BLAST
Alternative sequencei173 – 19220Missing in isoform 2 and isoform 3. 1 PublicationVSP_007536Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41663 mRNA. Translation: AAA97871.1.
RefSeqiNP_599163.2. NM_134336.2.
UniGeneiRn.226139.

Genome annotation databases

GeneIDi171297.
KEGGirno:171297.
UCSCiRGD:621119. rat. [Q62889-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41663 mRNA. Translation: AAA97871.1.
RefSeqiNP_599163.2. NM_134336.2.
UniGeneiRn.226139.

3D structure databases

ProteinModelPortaliQ62889.
SMRiQ62889. Positions 39-631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1561818.
STRINGi10116.ENSRNOP00000005077.

Protein family/group databases

MEROPSiS09.987.

PTM databases

PhosphoSiteiQ62889.

Proteomic databases

PaxDbiQ62889.
PRIDEiQ62889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171297.
KEGGirno:171297.
UCSCiRGD:621119. rat. [Q62889-1]

Organism-specific databases

CTDi54413.
RGDi621119. Nlgn3.

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ62889.
KOiK07378.
PhylomeDBiQ62889.

Miscellaneous databases

NextBioi622053.
PROiQ62889.

Gene expression databases

GenevestigatoriQ62889.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030024. NLGN3.
[Graphical view]
PANTHERiPTHR11559:SF145. PTHR11559:SF145. 1 hit.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structures, alternative splicing, and neurexin binding of multiple neuroligins."
    Ichtchenko K., Nguyen T., Suedhof T.C.
    J. Biol. Chem. 271:2676-2682(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), BLOCKAGE OF N-TERMINUS, TISSUE SPECIFICITY, INTERACTION WITH NEUREXIN 1-BETA; NEUREXIN 2-BETA AND NEUREXIN 3-BETA.
    Tissue: Forebrain.
  2. "Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia."
    Gilbert M., Smith J., Roskams A.J., Auld V.J.
    Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. "The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing."
    Comoletti D., De Jaco A., Jennings L.L., Flynn R.E., Gaietta G., Tsigelny I., Ellisman M.H., Taylor P.
    J. Neurosci. 24:4889-4893(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-451, SUBCELLULAR LOCATION, INTERACTION WITH NRXN1, IDENTIFICATION BY MASS SPECTROMETRY.
  4. Cited for: TISSUE SPECIFICITY.
  5. "Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses."
    Budreck E.C., Scheiffele P.
    Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion."
    Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D.
    Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
  7. "Splice form dependence of beta-neurexin/neuroligin binding interactions."
    Koehnke J., Katsamba P.S., Ahlsen G., Bahna F., Vendome J., Honig B., Shapiro L., Jin X.
    Neuron 67:61-74(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NRXN1.
  8. "Postsynaptic scaffolding molecules modulate the localization of neuroligins."
    Levinson J.N., Li R., Kang R., Moukhles H., El-Husseini A., Bamji S.X.
    Neuroscience 165:782-793(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNLGN3_RAT
AccessioniPrimary (citable) accession number: Q62889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.