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Q62889

- NLGN3_RAT

UniProt

Q62889 - NLGN3_RAT

Protein

Neuroligin-3

Gene

Nlgn3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.1 Publication

    GO - Molecular functioni

    1. cell adhesion molecule binding Source: BHF-UCL
    2. neurexin family protein binding Source: BHF-UCL
    3. receptor activity Source: BHF-UCL

    GO - Biological processi

    1. axon extension Source: BHF-UCL
    2. neuron cell-cell adhesion Source: BHF-UCL
    3. positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
    4. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
    5. positive regulation of synapse assembly Source: BHF-UCL
    6. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
    7. postsynaptic membrane assembly Source: BHF-UCL
    8. presynaptic membrane assembly Source: BHF-UCL
    9. receptor-mediated endocytosis Source: BHF-UCL
    10. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
    11. regulation of dendritic spine morphogenesis Source: BHF-UCL
    12. regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
    13. regulation of inhibitory postsynaptic membrane potential Source: BHF-UCL
    14. regulation of long-term synaptic potentiation Source: BHF-UCL
    15. regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
    16. regulation of respiratory gaseous exchange by neurological system process Source: BHF-UCL
    17. regulation of synaptic transmission Source: BHF-UCL
    18. regulation of synaptic transmission, glutamatergic Source: BHF-UCL
    19. regulation of terminal button organization Source: BHF-UCL
    20. rhythmic synaptic transmission Source: BHF-UCL
    21. social behavior Source: BHF-UCL
    22. synapse assembly Source: BHF-UCL
    23. synapse organization Source: MGI

    Keywords - Biological processi

    Cell adhesion

    Protein family/group databases

    MEROPSiS09.987.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroligin-3
    Alternative name(s):
    Gliotactin homolog
    Gene namesi
    Name:Nlgn3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621119. Nlgn3.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse
    Note: Detected at both glutamatergic and GABAergic synapses.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cell surface Source: BHF-UCL
    3. endocytic vesicle Source: BHF-UCL
    4. excitatory synapse Source: BHF-UCL
    5. inhibitory synapse Source: BHF-UCL
    6. integral component of plasma membrane Source: BHF-UCL
    7. synapse Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi451 – 4511R → C: Impaired cell surface expression, and reduced interaction with NRXN1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737Sequence AnalysisAdd
    BLAST
    Chaini38 – 848811Neuroligin-3PRO_0000008647Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi106 ↔ 141By similarity
    Disulfide bondi340 ↔ 351By similarity
    Disulfide bondi510 ↔ 544By similarity
    Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis
    Modified residuei792 – 7921PhosphotyrosineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ62889.
    PRIDEiQ62889.

    PTM databases

    PhosphoSiteiQ62889.

    Expressioni

    Tissue specificityi

    Detected in brain and on hippocampus neurons, especially at excitatory synapses. Detected in retina (at protein level). Expressed in brain, spinal chord and dorsal root ganglion.6 Publications

    Gene expression databases

    GenevestigatoriQ62889.

    Interactioni

    Subunit structurei

    Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) By similarity. Homodimer, and heterodimer with NLGN1 and NLGN2 By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-1561818.
    STRINGi10116.ENSRNOP00000005077.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62889.
    SMRiQ62889. Positions 39-631.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini38 – 709672ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini731 – 848118CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei710 – 73021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2272.
    HOGENOMiHOG000231424.
    HOVERGENiHBG008839.
    InParanoidiQ62889.
    KOiK07378.
    PhylomeDBiQ62889.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019819. Carboxylesterase_B_CS.
    IPR000460. Neuroligin.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR01090. NEUROLIGIN.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q62889-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK    50
    LRGARVPLPS EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT 100
    HFPPVCPQNI HTAVPEVMLP VWFTANLDIV ATYIQEPNED CLYLNVYVPT 150
    EDVKRISKEC ARKPNKKICR KGGSGAKKQG EDLADNDGDE DEDIRDSGAK 200
    PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG VLGFLSTGDQ 250
    AAKGNYGLLD QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS 300
    HHSEGLFQRA IIQSGSALSS WAVNYQPVKY TSLLADKVGC NVLDTVDMVD 350
    CLRQKSAKEL VEQDIQPARY HVAFGPVIDG DVIPDDPEIL MEQGEFLNYD 400
    IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL YGYPEGKDTL 450
    RETIKFMYTD WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP 500
    TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD LFPCNFSKND 550
    VMLSAVVMTY WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD 600
    QLYLHIGLKP RVRDHYRATK VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP 650
    DTTHSSHITR RPNGKTWSTK RPAISPAYSN ENAPGSWNGD QDAGPLLVEN 700
    PRDYSTELSV TIAVGASLLF LNVLAFAALY YRKDKRRQEP LRQPSPQRGT 750
    GAPELGTAPE EELAALQLGP THHECEAGPP HDTLRLTALP DYTLTLRRSP 800
    DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP NSHSTTRV 848
    Length:848
    Mass (Da):93,888
    Last modified:November 1, 1996 - v1
    Checksum:i7520653B3253E750
    GO
    Isoform 2 (identifier: Q62889-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         153-172: Missing.
         173-192: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:808
    Mass (Da):89,531
    Checksum:iC2B71B50BEB9B541
    GO
    Isoform 3 (identifier: Q62889-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         173-192: Missing.

    Show »
    Length:828
    Mass (Da):91,856
    Checksum:i3CD9FC2E5B84472C
    GO
    Isoform 4 (identifier: Q62889-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         153-172: Missing.

    Show »
    Length:828
    Mass (Da):91,563
    Checksum:iD612DF45CF916C8B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei153 – 17220Missing in isoform 2 and isoform 4. 1 PublicationVSP_007535Add
    BLAST
    Alternative sequencei173 – 19220Missing in isoform 2 and isoform 3. 1 PublicationVSP_007536Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41663 mRNA. Translation: AAA97871.1.
    RefSeqiNP_599163.2. NM_134336.2.
    UniGeneiRn.226139.

    Genome annotation databases

    GeneIDi171297.
    KEGGirno:171297.
    UCSCiRGD:621119. rat. [Q62889-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41663 mRNA. Translation: AAA97871.1 .
    RefSeqi NP_599163.2. NM_134336.2.
    UniGenei Rn.226139.

    3D structure databases

    ProteinModelPortali Q62889.
    SMRi Q62889. Positions 39-631.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1561818.
    STRINGi 10116.ENSRNOP00000005077.

    Protein family/group databases

    MEROPSi S09.987.

    PTM databases

    PhosphoSitei Q62889.

    Proteomic databases

    PaxDbi Q62889.
    PRIDEi Q62889.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171297.
    KEGGi rno:171297.
    UCSCi RGD:621119. rat. [Q62889-1 ]

    Organism-specific databases

    CTDi 54413.
    RGDi 621119. Nlgn3.

    Phylogenomic databases

    eggNOGi COG2272.
    HOGENOMi HOG000231424.
    HOVERGENi HBG008839.
    InParanoidi Q62889.
    KOi K07378.
    PhylomeDBi Q62889.

    Miscellaneous databases

    NextBioi 622053.
    PROi Q62889.

    Gene expression databases

    Genevestigatori Q62889.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019819. Carboxylesterase_B_CS.
    IPR000460. Neuroligin.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR01090. NEUROLIGIN.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    PROSITEi PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structures, alternative splicing, and neurexin binding of multiple neuroligins."
      Ichtchenko K., Nguyen T., Suedhof T.C.
      J. Biol. Chem. 271:2676-2682(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), BLOCKAGE OF N-TERMINUS, TISSUE SPECIFICITY, INTERACTION WITH NEUREXIN 1-BETA; NEUREXIN 2-BETA AND NEUREXIN 3-BETA.
      Tissue: Forebrain.
    2. "Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia."
      Gilbert M., Smith J., Roskams A.J., Auld V.J.
      Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. "The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing."
      Comoletti D., De Jaco A., Jennings L.L., Flynn R.E., Gaietta G., Tsigelny I., Ellisman M.H., Taylor P.
      J. Neurosci. 24:4889-4893(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-451, SUBCELLULAR LOCATION, INTERACTION WITH NRXN1, IDENTIFICATION BY MASS SPECTROMETRY.
    4. Cited for: TISSUE SPECIFICITY.
    5. "Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses."
      Budreck E.C., Scheiffele P.
      Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion."
      Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D.
      Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    7. "Splice form dependence of beta-neurexin/neuroligin binding interactions."
      Koehnke J., Katsamba P.S., Ahlsen G., Bahna F., Vendome J., Honig B., Shapiro L., Jin X.
      Neuron 67:61-74(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NRXN1.
    8. "Postsynaptic scaffolding molecules modulate the localization of neuroligins."
      Levinson J.N., Li R., Kang R., Moukhles H., El-Husseini A., Bamji S.X.
      Neuroscience 165:782-793(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiNLGN3_RAT
    AccessioniPrimary (citable) accession number: Q62889
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 23, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3