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Q62889 (NLGN3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroligin-3
Alternative name(s):
Gliotactin homolog
Gene names
Name:Nlgn3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Ref.5

Subunit structure

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) By similarity. Homodimer, and heterodimer with NLGN1 and NLGN2 By similarity. Ref.1 Ref.3 Ref.7

Subcellular location

Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse. Note: Detected at both glutamatergic and GABAergic synapses. Ref.3 Ref.5 Ref.8

Tissue specificity

Detected in brain and on hippocampus neurons, especially at excitatory synapses. Detected in retina (at protein level). Expressed in brain, spinal chord and dorsal root ganglion. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.8

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon extension

Inferred from mutant phenotype PubMed 19406211. Source: BHF-UCL

neuron cell-cell adhesion

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of excitatory postsynaptic membrane potential

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synapse assembly

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

positive regulation of synaptic transmission, glutamatergic

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

presynaptic membrane assembly

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

receptor-mediated endocytosis

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

regulation of N-methyl-D-aspartate selective glutamate receptor activity

Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL

regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of dendritic spine morphogenesis

Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL

regulation of excitatory postsynaptic membrane potential

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of inhibitory postsynaptic membrane potential

Inferred from direct assay PubMed 19406211. Source: BHF-UCL

regulation of long-term synaptic potentiation

Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL

regulation of respiratory gaseous exchange by neurological system process

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of synaptic transmission

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of synaptic transmission, glutamatergic

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of terminal button organization

Inferred from sequence or structural similarity PubMed 21808020. Source: BHF-UCL

rhythmic synaptic transmission

Inferred from mutant phenotype PubMed 19406211. Source: BHF-UCL

social behavior

Inferred from sequence or structural similarity. Source: BHF-UCL

synapse assembly

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

synapse organization

Inferred from direct assay PubMed 15681343. Source: MGI

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

endocytic vesicle

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

excitatory synapse

Inferred from direct assay Ref.8. Source: BHF-UCL

inhibitory synapse

Inferred by curator PubMed 19406211. Source: BHF-UCL

integral component of plasma membrane

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

synapse

Inferred from direct assay PubMed 15681343. Source: MGI

   Molecular_functioncell adhesion molecule binding

Inferred from physical interaction Ref.3. Source: BHF-UCL

neurexin family protein binding

Inferred from physical interaction Ref.3. Source: BHF-UCL

receptor activity

Inferred from direct assay PubMed 15797875. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62889-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62889-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-172: Missing.
     173-192: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q62889-3)

The sequence of this isoform differs from the canonical sequence as follows:
     173-192: Missing.
Isoform 4 (identifier: Q62889-4)

The sequence of this isoform differs from the canonical sequence as follows:
     153-172: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3737 Potential
Chain38 – 848811Neuroligin-3
PRO_0000008647

Regions

Topological domain38 – 709672Extracellular Potential
Transmembrane710 – 73021Helical; Potential
Topological domain731 – 848118Cytoplasmic Potential

Amino acid modifications

Modified residue7921Phosphotyrosine By similarity
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation5451N-linked (GlcNAc...) Potential
Disulfide bond106 ↔ 141 By similarity
Disulfide bond340 ↔ 351 By similarity
Disulfide bond510 ↔ 544 By similarity

Natural variations

Alternative sequence153 – 17220Missing in isoform 2 and isoform 4.
VSP_007535
Alternative sequence173 – 19220Missing in isoform 2 and isoform 3.
VSP_007536

Experimental info

Mutagenesis4511R → C: Impaired cell surface expression, and reduced interaction with NRXN1. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7520653B3253E750

FASTA84893,888
        10         20         30         40         50         60 
MWLQLGLPSL SLSPTPTVGR SLCLILWFLS LVLRASTQAP APTVNTHFGK LRGARVPLPS 

        70         80         90        100        110        120 
EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PSWSGIRNAT HFPPVCPQNI HTAVPEVMLP 

       130        140        150        160        170        180 
VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDVKRISKEC ARKPNKKICR KGGSGAKKQG 

       190        200        210        220        230        240 
EDLADNDGDE DEDIRDSGAK PVMVYIHGGS YMEGTGNMID GSVLASYGNV IVITLNYRVG 

       250        260        270        280        290        300 
VLGFLSTGDQ AAKGNYGLLD QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS 

       310        320        330        340        350        360 
HHSEGLFQRA IIQSGSALSS WAVNYQPVKY TSLLADKVGC NVLDTVDMVD CLRQKSAKEL 

       370        380        390        400        410        420 
VEQDIQPARY HVAFGPVIDG DVIPDDPEIL MEQGEFLNYD IMLGVNQGEG LKFVEGVVDP 

       430        440        450        460        470        480 
EDGVSGTDFD YSVSNFVDNL YGYPEGKDTL RETIKFMYTD WADRDNPETR RKTLVALFTD 

       490        500        510        520        530        540 
HQWVEPSVVT ADLHARYGSP TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD 

       550        560        570        580        590        600 
LFPCNFSKND VMLSAVVMTY WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD 

       610        620        630        640        650        660 
QLYLHIGLKP RVRDHYRATK VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP DTTHSSHITR 

       670        680        690        700        710        720 
RPNGKTWSTK RPAISPAYSN ENAPGSWNGD QDAGPLLVEN PRDYSTELSV TIAVGASLLF 

       730        740        750        760        770        780 
LNVLAFAALY YRKDKRRQEP LRQPSPQRGT GAPELGTAPE EELAALQLGP THHECEAGPP 

       790        800        810        820        830        840 
HDTLRLTALP DYTLTLRRSP DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP 


NSHSTTRV 

« Hide

Isoform 2 [UniParc].

Checksum: C2B71B50BEB9B541
Show »

FASTA80889,531
Isoform 3 [UniParc].

Checksum: 3CD9FC2E5B84472C
Show »

FASTA82891,856
Isoform 4 [UniParc].

Checksum: D612DF45CF916C8B
Show »

FASTA82891,563

References

[1]"Structures, alternative splicing, and neurexin binding of multiple neuroligins."
Ichtchenko K., Nguyen T., Suedhof T.C.
J. Biol. Chem. 271:2676-2682(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), BLOCKAGE OF N-TERMINUS, TISSUE SPECIFICITY, INTERACTION WITH NEUREXIN 1-BETA; NEUREXIN 2-BETA AND NEUREXIN 3-BETA.
Tissue: Forebrain.
[2]"Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia."
Gilbert M., Smith J., Roskams A.J., Auld V.J.
Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[3]"The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing."
Comoletti D., De Jaco A., Jennings L.L., Flynn R.E., Gaietta G., Tsigelny I., Ellisman M.H., Taylor P.
J. Neurosci. 24:4889-4893(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-451, SUBCELLULAR LOCATION, INTERACTION WITH NRXN1, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"Neuroligins determine synapse maturation and function."
Varoqueaux F., Aramuni G., Rawson R.L., Mohrmann R., Missler M., Gottmann K., Zhang W., Sudhof T.C., Brose N.
Neuron 51:741-754(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses."
Budreck E.C., Scheiffele P.
Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion."
Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D.
Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[7]"Splice form dependence of beta-neurexin/neuroligin binding interactions."
Koehnke J., Katsamba P.S., Ahlsen G., Bahna F., Vendome J., Honig B., Shapiro L., Jin X.
Neuron 67:61-74(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NRXN1.
[8]"Postsynaptic scaffolding molecules modulate the localization of neuroligins."
Levinson J.N., Li R., Kang R., Moukhles H., El-Husseini A., Bamji S.X.
Neuroscience 165:782-793(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U41663 mRNA. Translation: AAA97871.1.
RefSeqNP_599163.2. NM_134336.2.
UniGeneRn.226139.

3D structure databases

ProteinModelPortalQ62889.
SMRQ62889. Positions 39-631.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1561818.
STRING10116.ENSRNOP00000005077.

Protein family/group databases

MEROPSS09.987.

PTM databases

PhosphoSiteQ62889.

Proteomic databases

PaxDbQ62889.
PRIDEQ62889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID171297.
KEGGrno:171297.
UCSCRGD:621119. rat. [Q62889-1]

Organism-specific databases

CTD54413.
RGD621119. Nlgn3.

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000231424.
HOVERGENHBG008839.
InParanoidQ62889.
KOK07378.
PhylomeDBQ62889.

Gene expression databases

GenevestigatorQ62889.

Family and domain databases

Gene3D3.40.50.1820. 2 hits.
InterProIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Neuroligin.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR01090. NEUROLIGIN.
SUPFAMSSF53474. SSF53474. 2 hits.
PROSITEPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622053.
PROQ62889.

Entry information

Entry nameNLGN3_RAT
AccessionPrimary (citable) accession number: Q62889
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families