3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4

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Reviewed, UniProtKB/Swiss-Prot Q62878 (3BHS4_RAT)

Last modified June 16, 2009. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4
Alternative name(s):
    3-beta-HSD IV
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD⁺ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Skin, placenta, also detectable in ovary and adrenal gland.
Sequence similarities	Belongs to the 3-beta-HSD family.

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Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3(or 17)beta-hydroxysteroid dehydrogenase activity Ref.1 Traceable author statement. Source: RGD 3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 373

372

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4

PRO_0000087790

Regions

Transmembrane

288 – 308

Potential

Sites

Active site

155

Proton acceptor By similarity

Binding site

159

NAD By similarity

Experimental info

Sequence conflict

208

S → N in AAA40606. Ref.1

Sequence conflict

341

K → E in AAA40606. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q62878-1 [UniParc].

Last modified April 3, 2007. Version 4.
Checksum: 1B8A0FB794D05540
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FASTA

373

41,957

        10         20         30         40         50         60 
MPGWSCLVTG AGGFLGQRIV QLLVQEKDLK EVRVLDKVFR PETREEFFNL GTSIKVTVLE 

        70         80         90        100        110        120 
GDILDTQCLR RACQGISVVI HTAALIDVTG VNPRQTILDV NLKGTQNLLE ACVQASVPAF 

       130        140        150        160        170        180 
IYCSTVDVAG PNSYKKIILN GHEEEHHEST WSNPYPYSKK MAEKAVLAAN GSILKNGGTL 

       190        200        210        220        230        240 
HTCALRPMYI YGERSPFLSV MILAALKSKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL 

       250        260        270        280        290        300 
RDPKKSQNVQ GQFYYISDDT PHQSYDDLNY TLSKEWGLHL DSSWSLPLPL LYWLAFLLEI 

       310        320        330        340        350        360 
VSFFLHPVYN YRPSFNRHLV TLSNSKFTFS YKKAQRDLGY KPLVSWEEAK QKTSEWIGTL 

       370 
VEQHRETLDT KSQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and tissue-specific expression of a novel member of the rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) family. The exclusive 3 beta-HSD gene expression in the skin." Simard J., Couet J., Durocher F., Labrie Y., Sanchez R., Breton N., Turgeon C., Labrie F. J. Biol. Chem. 268:19659-19668(1993) [PubMed: 7690038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

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Cross-references

Sequence databases
	L17138 mRNA. Translation: AAA40606.1. BC089937 mRNA. Translation: AAH89937.1.
IPI	IPI00555268.
PIR	A48769.
RefSeq	NP_058961.4.
UniGene	Rn.109394
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000019441. Rattus norvegicus. [Contig view]
GeneID	29632.
KEGG	rno:29632.
NMPDR	fig\|10116.3.peg.16692.
Organism-specific databases
RGD	67377. Hsd3b6.
Phylogenomic databases
HOVERGEN	Q62878.
OMA	Q62878. LQDPKKA.
Enzyme and pathway databases
BRENDA	1.1.1.145. 248. 5.3.3.1. 248.
Gene expression databases
ArrayExpress	Q62878.
GermOnline	ENSRNOG00000019441. Rattus norvegicus.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609864.

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Entry information

Entry name

3BHS4_RAT

Accession

Primary (citable) accession number: Q62878
Secondary accession number(s): Q5FVK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 3, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents