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Protein

Cytoplasmic dynein 1 intermediate chain 2

Gene

Dync1i2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.1 Publication

GO - Molecular functioni

  • motor activity Source: UniProtKB-KW
  • protein complex binding Source: RGD

GO - Biological processi

  • microtubule-based movement Source: RGD
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 2
Alternative name(s):
Cytoplasmic dynein intermediate chain 2
Dynein intermediate chain 2, cytosolic
Short name:
DH IC-2
Gene namesi
Name:Dync1i2
Synonyms:Dnci2, Dncic2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620174. Dync1i2.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90S → A: No effect on interaction with DCTN1 (mimicks dephosphorylated form). 1 Publication1
Mutagenesisi90S → D: Impairs interaction with DCTN1 (mimicks phosphorylated form). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001146572 – 638Cytoplasmic dynein 1 intermediate chain 2Add BLAST637

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei51PhosphoserineCombined sources1
Modified residuei90Phosphoserine1 Publication1
Modified residuei95PhosphothreonineBy similarity1
Modified residuei97PhosphoserineBy similarity1
Modified residuei101PhosphoserineBy similarity1
Modified residuei104PhosphoserineCombined sources1

Post-translational modificationi

The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62871.
PeptideAtlasiQ62871.
PRIDEiQ62871.

PTM databases

iPTMnetiQ62871.
PhosphoSitePlusiQ62871.

Expressioni

Tissue specificityi

Skeletal muscle, testis, kidney, brain, heart and spleen.

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Isoform 1, isoform 2 and isoform 3 interact with DYNLT3. Isoform 1, isoform 2 and isoform 3 interact with DYNLT1. Interacts (dephosphorylated at Ser-90) with DCTN1.By similarity7 Publications

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

DIPiDIP-36880N.
IntActiQ62871. 2 interactors.
STRINGi10116.ENSRNOP00000060919.

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi144 – 148Combined sources5
Beta strandi155 – 161Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PG1X-ray2.80I/J/K/L132-164[»]
ProteinModelPortaliQ62871.
SMRiQ62871.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62871.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati277 – 326WD 1Add BLAST50
Repeati330 – 370WD 2Add BLAST41
Repeati379 – 420WD 3Add BLAST42
Repeati429 – 469WD 4Add BLAST41
Repeati474 – 519WD 5Add BLAST46
Repeati568 – 607WD 6Add BLAST40

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiQ62871.
PhylomeDBiQ62871.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 2A (identifier: Q62871-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDKSELKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAAVSVQEE
60 70 80 90 100
SDLEKKRREA EALLQSMGLT TDSPIVFSEH WVPPPMSPSS KSVSTPSEAG
110 120 130 140 150
SQDSGDGAVG SRTLHWDTDP SALQLHSDSD LGRGPIKLGM AKITQVDFPP
160 170 180 190 200
REIVTYTKET QTPVTAQPKE DEEEEDDVAA PKPPVEPEEE KILKKDEEND
210 220 230 240 250
SKAPPHELTE EEKQQILHSE EFLSFFDHST RIVERALSEQ INIFFDYSGR
260 270 280 290 300
DLEDKEGEIQ AGAKLSLNRQ FFDERWSKHR VVSCLDWSSQ YPELLVASYN
310 320 330 340 350
NNEEAPHEPD GVALVWNMKY KKTTPEYVFH CQSAVMSATF AKFHPNLVVG
360 370 380 390 400
GTYSGQIVLW DNRSNKRTPV QRTPLSAAAH THPVYCVNVV GTQNAHNLIS
410 420 430 440 450
ISTDGKICSW SLDMLSHPQD SMELVHKQSK AVAVTSMSFP VGDVNNFVVG
460 470 480 490 500
SEEGSVYTAC RHGSKAGISE MFEGHQGPIT GIHCHAAVGA VDFSHLFVTS
510 520 530 540 550
SFDWTVKLWS TKNNKPLYSF EDNSDYVYDV IGSPTHPALF ACVDGMGRLD
560 570 580 590 600
LWNLNNDTEV PTASISVEGN PALNRVRWTH SGREIAVGDS EGQIVIYDVG
610 620 630
EQIAVPRNDE WARFGRTLAE INASRADAEE EAATRIPA
Length:638
Mass (Da):71,178
Last modified:November 1, 1997 - v1
Checksum:i0A655543CE266E95
GO
Isoform 2B (identifier: Q62871-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.

Show »
Length:632
Mass (Da):70,392
Checksum:iCEDB6F3F71D21952
GO
Isoform 2C (identifier: Q62871-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
     113-132: Missing.

Show »
Length:612
Mass (Da):68,202
Checksum:i73624E49743575E4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00133977 – 82Missing in isoform 2B and isoform 2C. 1 Publication6
Alternative sequenceiVSP_001340113 – 132Missing in isoform 2C. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39044 mRNA. Translation: AAA89163.1.
U39045 mRNA. Translation: AAA89164.1.
U39046 mRNA. Translation: AAA89165.1.
UniGeneiRn.11014.

Genome annotation databases

UCSCiRGD:620174. rat. [Q62871-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39044 mRNA. Translation: AAA89163.1.
U39045 mRNA. Translation: AAA89164.1.
U39046 mRNA. Translation: AAA89165.1.
UniGeneiRn.11014.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PG1X-ray2.80I/J/K/L132-164[»]
ProteinModelPortaliQ62871.
SMRiQ62871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36880N.
IntActiQ62871. 2 interactors.
STRINGi10116.ENSRNOP00000060919.

PTM databases

iPTMnetiQ62871.
PhosphoSitePlusiQ62871.

Proteomic databases

PaxDbiQ62871.
PeptideAtlasiQ62871.
PRIDEiQ62871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620174. rat. [Q62871-1]

Organism-specific databases

RGDi620174. Dync1i2.

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiQ62871.
PhylomeDBiQ62871.

Miscellaneous databases

EvolutionaryTraceiQ62871.
PROiQ62871.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDC1I2_RAT
AccessioniPrimary (citable) accession number: Q62871
Secondary accession number(s): Q62872, Q62873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.