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Q62865

- PDE3A_RAT

UniProt

Q62865 - PDE3A_RAT

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Protein
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Gene
Pde3a
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes By similarity.

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Inhibited by cGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei752 – 7521Proton donor By similarity
Metal bindingi756 – 7561Divalent metal cation 1 By similarity
Metal bindingi836 – 8361Divalent metal cation 1 By similarity
Metal bindingi837 – 8371Divalent metal cation 1 By similarity
Metal bindingi837 – 8371Divalent metal cation 2 By similarity
Metal bindingi950 – 9501Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
  2. cAMP binding Source: RGD
  3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: RGD
  4. cyclic-nucleotide phosphodiesterase activity Source: RGD
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: RGD
  2. cAMP-mediated signaling Source: Ensembl
  3. cGMP-mediated signaling Source: Ensembl
  4. cellular response to cGMP Source: Ensembl
  5. cellular response to transforming growth factor beta stimulus Source: Ensembl
  6. diterpenoid metabolic process Source: RGD
  7. negative regulation of apoptotic process Source: RGD
  8. negative regulation of vascular permeability Source: Ensembl
  9. oocyte maturation Source: RGD
  10. positive regulation of oocyte development Source: Ensembl
  11. positive regulation of vascular permeability Source: Ensembl
  12. regulation of meiosis Source: Ensembl
  13. response to cAMP Source: RGD
  14. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
Alternative name(s):
Cyclic GMP-inhibited phosphodiesterase A
Short name:
CGI-PDE A
Gene namesi
Name:Pde3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi61942. Pde3a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei62 – 8221Helical; Reviewed prediction
Add
BLAST
Transmembranei127 – 14721Helical; Reviewed prediction
Add
BLAST
Transmembranei157 – 17721Helical; Reviewed prediction
Add
BLAST
Transmembranei182 – 20221Helical; Reviewed prediction
Add
BLAST
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Transmembranei229 – 24921Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytosol Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase A
PRO_0000198801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei310 – 3101Phosphoserine By similarity
Modified residuei520 – 5201Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ62865.

Expressioni

Gene expression databases

GenevestigatoriQ62865.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000032282.

Structurei

3D structure databases

ProteinModelPortaliQ62865.
SMRiQ62865. Positions 677-1087.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni728 – 1086359Catalytic By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145074.
GeneTreeiENSGT00750000117528.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ62865.
KOiK13296.
OMAiPETMMFL.
OrthoDBiEOG7KDF95.
PhylomeDBiQ62865.
TreeFamiTF329631.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62865-1 [UniParc]FASTAAdd to Basket

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MAVRGEAAQD WAKPGLRGPS PAPVARGDHR CRGGSPSSPR GSGCCWRALA     50
LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELES SQEAAAEEEE 100
EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA 150
GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW 200
LVLRLRLGVL MVALTSALRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR 250
YAEQLLPQCS GPAPPRERFG SQSSARTKEE IPGWKRRRRS SSVVAGEMSG 300
CGGKSHRRTS LPCIPREQLM GHSEWDHKRG SRGSQSGTSV TVDIAVMGEA 350
HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE 400
NYTCSDSEEG LEKDKLAIPK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL 450
EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APVLMTLTKS RSFTSSYAVS 500
AANHVKAKKQ NRPGGLDKIS PVPSPSSSPP QGSPTSSPVS GIASVQFPES 550
PEVTTKRGPG SHRALTYTQS APDLSPQIPP SPVICSSCGR PYSQGNPADG 600
PSERSGPAMQ KPNRTDDTSQ VTSDYETNNN SDSSDILQND EEAECQREPL 650
RKASACGTYT PQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLV 700
ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP 750
YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG 800
YVFSKAYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA 850
TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 900
VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD 950
INGPAKCKDL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL 1000
QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH 1050
EEETCENSEA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE 1100
NQAPDQAPLQ HSSEQIQAIK EEEEEKGKPR AEETLAPQPD L 1141
Length:1,141
Mass (Da):124,301
Last modified:November 1, 1996 - v1
Checksum:iA333DFB44F6F33F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38179 mRNA. Translation: AAA84964.1.
RefSeqiNP_059033.1. NM_017337.1.
UniGeneiRn.44403.

Genome annotation databases

EnsembliENSRNOT00000032843; ENSRNOP00000032282; ENSRNOG00000025042.
GeneIDi50678.
KEGGirno:50678.
UCSCiRGD:61942. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38179 mRNA. Translation: AAA84964.1 .
RefSeqi NP_059033.1. NM_017337.1.
UniGenei Rn.44403.

3D structure databases

ProteinModelPortali Q62865.
SMRi Q62865. Positions 677-1087.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000032282.

Chemistry

BindingDBi Q62865.

Proteomic databases

PRIDEi Q62865.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000032843 ; ENSRNOP00000032282 ; ENSRNOG00000025042 .
GeneIDi 50678.
KEGGi rno:50678.
UCSCi RGD:61942. rat.

Organism-specific databases

CTDi 5139.
RGDi 61942. Pde3a.

Phylogenomic databases

eggNOGi NOG145074.
GeneTreei ENSGT00750000117528.
HOGENOMi HOG000060144.
HOVERGENi HBG053541.
InParanoidi Q62865.
KOi K13296.
OMAi PETMMFL.
OrthoDBi EOG7KDF95.
PhylomeDBi Q62865.
TreeFami TF329631.

Miscellaneous databases

NextBioi 610514.
PROi Q62865.

Gene expression databases

Genevestigatori Q62865.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Expression and characterization of deletion recombinants of two cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3)."
    He R., Komas N., Ekholm D., Murata T., Taira M., Hockman S.C., Degerman E., Manganiello V.C.
    Cell Biochem. Biophys. 29:89-111(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adipose tissue.

Entry informationi

Entry nameiPDE3A_RAT
AccessioniPrimary (citable) accession number: Q62865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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