Reviewed, UniProtKB/Swiss-Prot Q62865 (PDE3A_RAT)

Names and origin

Protein names

Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
      Short name=CGI-PDE A

Gene names

Name:

Pde3a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP).
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Enzyme regulation	Inhibited by cGMP.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cAMP catabolic process Inferred from direct assay. Source: RGD negative regulation of apoptosis Inferred from mutant phenotype. Source: RGD oocyte maturation Inferred from expression pattern. Source: RGD response to cAMP Inferred from expression pattern. Source: RGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: RGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: RGD
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from direct assay. Source: RGD cAMP binding Inferred from direct assay. Source: RGD cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	cGMP-inhibited 3',5'-cyclic phosphodiesterase A		PRO_0000198801
Amino acid modifications
	Modified residue	310	1	Phosphoserine By similarity
	Modified residue	425	1	Phosphoserine By similarity
	Modified residue	437	1	Phosphothreonine By similarity
	Modified residue	495	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62865-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A333DFB44F6F33F3	FASTA	1,141	124,301
	10 20 30 40 50 60 MAVRGEAAQD WAKPGLRGPS PAPVARGDHR CRGGSPSSPR GSGCCWRALA LQPLRRSPQL 70 80 90 100 110 120 SSALCAGSLS VLLALLVRLV GGEVGGELES SQEAAAEEEE EEGARGGVFP GPRGGAPGGG 130 140 150 160 170 180 AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA GVRLPLAVAL LAACCAGEAL VQLSLGVGDG 190 200 210 220 230 240 RLLSLPAAGV LLSCLGGATW LVLRLRLGVL MVALTSALRT VALVSLERFK VAWRPYLAYL 250 260 270 280 290 300 AAVLGLLLAR YAEQLLPQCS GPAPPRERFG SQSSARTKEE IPGWKRRRRS SSVVAGEMSG 310 320 330 340 350 360 CGGKSHRRTS LPCIPREQLM GHSEWDHKRG SRGSQSGTSV TVDIAVMGEA HGLITDLLAD 370 380 390 400 410 420 PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE NYTCSDSEEG LEKDKLAIPK 430 440 450 460 470 480 RLRRSLPPGL LRRVSSTWTT TTSATGLPTL EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN 490 500 510 520 530 540 APVLMTLTKS RSFTSSYAVS AANHVKAKKQ NRPGGLDKIS PVPSPSSSPP QGSPTSSPVS 550 560 570 580 590 600 GIASVQFPES PEVTTKRGPG SHRALTYTQS APDLSPQIPP SPVICSSCGR PYSQGNPADG 610 620 630 640 650 660 PSERSGPAMQ KPNRTDDTSQ VTSDYETNNN SDSSDILQND EEAECQREPL RKASACGTYT 670 680 690 700 710 720 PQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLV ENIGRKCGRI LSQVSYRLFE 730 740 750 760 770 780 DMGLFEAFKI PVREFMNYFH ALEIGYRDIP YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG 790 800 810 820 830 840 DHGSASDSDS DSGFTHGHMG YVFSKAYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH 850 860 870 880 890 900 PGRTNAFLVA TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 910 920 930 940 950 960 VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD INGPAKCKDL 970 980 990 1000 1010 1020 HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL QESFISHIVG PLCHSYDSAG 1030 1040 1050 1060 1070 1080 LMPGKWVDDS DDSGDTDDPE EEEEEAETPH EEETCENSEA PRKKSFKRRR IYCQITQHLL 1090 1100 1110 1120 1130 1140 QNHMMWKKVI EEEQCLSGTE NQAPDQAPLQ HSSEQIQAIK EEEEEKGKPR AEETLAPQPD L

References

[1]	"Expression and characterization of deletion recombinants of two cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3)." He R., Komas N., Ekholm D., Murata T., Taira M., Hockman S.C., Degerman E., Manganiello V.C. Cell Biochem. Biophys. 29:89-111(1998) [PubMed: 9631240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38179 mRNA. Translation: AAA84964.1.
IPI	IPI00209261.
RefSeq	NP_059033.1.
UniGene	Rn.44403
3D structure databases
HSSP	HSSP built from PDB template 1MKD based on UniProtKB Q08499.
SMR	Q62865. Positions 677-1049.
ModBase	Search...
Protein-protein interaction databases
STRING	Q62865.
Genome annotation databases
Ensembl	ENSRNOT00000032843; ENSRNOP00000032282; ENSRNOG00000025042; Rattus norvegicus. [Genome view]
GeneID	50678.
KEGG	rno:50678.
Organism-specific databases
CTD	50678.
RGD	61942. Pde3a.
Phylogenomic databases
HOVERGEN	Q62865.
OMA	DSGFTHG.
Enzyme and pathway databases
BRENDA	3.1.4.17. 248.
Gene expression databases
ArrayExpress	Q62865.
Genevestigator	Q62865.
GermOnline	ENSRNOG00000025042. Rattus norvegicus.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	610514.

Entry information

Entry name

PDE3A_RAT

Accession

Primary (citable) accession number: Q62865

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)