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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei752Proton donorBy similarity1
Metal bindingi756Divalent metal cation 1By similarity1
Metal bindingi836Divalent metal cation 1By similarity1
Metal bindingi837Divalent metal cation 1By similarity1
Metal bindingi837Divalent metal cation 2By similarity1
Metal bindingi950Divalent metal cation 1By similarity1

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
  • cAMP binding Source: RGD
  • cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: RGD
  • cyclic-nucleotide phosphodiesterase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-418457 cGMP effects
R-RNO-418555 G alpha (s) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
Alternative name(s):
Cyclic GMP-inhibited phosphodiesterase A
Short name:
CGI-PDE A
Gene namesi
Name:Pde3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi61942 Pde3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988011 – 1141cGMP-inhibited 3',5'-cyclic phosphodiesterase AAdd BLAST1141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei310PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei520PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1
Modified residuei533PhosphoserineBy similarity1
Modified residuei1033PhosphoserineBy similarity1
Modified residuei1036PhosphothreonineBy similarity1
Cross-linki1120Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62865
PRIDEiQ62865

PTM databases

PhosphoSitePlusiQ62865

Expressioni

Gene expression databases

BgeeiENSRNOG00000025042
GenevisibleiQ62865 RN

Interactioni

Protein-protein interaction databases

IntActiQ62865, 1 interactor
STRINGi10116.ENSRNOP00000032282

Structurei

3D structure databases

ProteinModelPortaliQ62865
SMRiQ62865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini674 – 1093PDEasePROSITE-ProRule annotationAdd BLAST420

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEGG Eukaryota
ENOG410XT2V LUCA
GeneTreeiENSGT00760000119066
HOGENOMiHOG000060144
HOVERGENiHBG053541
InParanoidiQ62865
KOiK19021
OMAiPNEEETC
OrthoDBiEOG091G0BTI
PhylomeDBiQ62865
TreeFamiTF329631

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q62865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRGEAAQD WAKPGLRGPS PAPVARGDHR CRGGSPSSPR GSGCCWRALA
60 70 80 90 100
LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELES SQEAAAEEEE
110 120 130 140 150
EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA
160 170 180 190 200
GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW
210 220 230 240 250
LVLRLRLGVL MVALTSALRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR
260 270 280 290 300
YAEQLLPQCS GPAPPRERFG SQSSARTKEE IPGWKRRRRS SSVVAGEMSG
310 320 330 340 350
CGGKSHRRTS LPCIPREQLM GHSEWDHKRG SRGSQSGTSV TVDIAVMGEA
360 370 380 390 400
HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE
410 420 430 440 450
NYTCSDSEEG LEKDKLAIPK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL
460 470 480 490 500
EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APVLMTLTKS RSFTSSYAVS
510 520 530 540 550
AANHVKAKKQ NRPGGLDKIS PVPSPSSSPP QGSPTSSPVS GIASVQFPES
560 570 580 590 600
PEVTTKRGPG SHRALTYTQS APDLSPQIPP SPVICSSCGR PYSQGNPADG
610 620 630 640 650
PSERSGPAMQ KPNRTDDTSQ VTSDYETNNN SDSSDILQND EEAECQREPL
660 670 680 690 700
RKASACGTYT PQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLV
710 720 730 740 750
ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP
760 770 780 790 800
YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG
810 820 830 840 850
YVFSKAYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA
860 870 880 890 900
TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL
910 920 930 940 950
VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD
960 970 980 990 1000
INGPAKCKDL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL
1010 1020 1030 1040 1050
QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH
1060 1070 1080 1090 1100
EEETCENSEA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE
1110 1120 1130 1140
NQAPDQAPLQ HSSEQIQAIK EEEEEKGKPR AEETLAPQPD L
Length:1,141
Mass (Da):124,301
Last modified:November 1, 1996 - v1
Checksum:iA333DFB44F6F33F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38179 mRNA Translation: AAA84964.1
RefSeqiNP_059033.1, NM_017337.1
UniGeneiRn.44403

Genome annotation databases

EnsembliENSRNOT00000032843; ENSRNOP00000032282; ENSRNOG00000025042
GeneIDi50678
KEGGirno:50678
UCSCiRGD:61942 rat

Similar proteinsi

Entry informationi

Entry nameiPDE3A_RAT
AccessioniPrimary (citable) accession number: Q62865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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