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Q62865

- PDE3A_RAT

UniProt

Q62865 - PDE3A_RAT

Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by cGMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei752 – 7521Proton donorBy similarity
    Metal bindingi756 – 7561Divalent metal cation 1By similarity
    Metal bindingi836 – 8361Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 2By similarity
    Metal bindingi950 – 9501Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
    2. cAMP binding Source: RGD
    3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: RGD
    4. cyclic-nucleotide phosphodiesterase activity Source: RGD
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: RGD
    2. cAMP-mediated signaling Source: Ensembl
    3. cellular response to cGMP Source: Ensembl
    4. cellular response to transforming growth factor beta stimulus Source: Ensembl
    5. cGMP-mediated signaling Source: Ensembl
    6. diterpenoid metabolic process Source: RGD
    7. negative regulation of apoptotic process Source: RGD
    8. negative regulation of vascular permeability Source: Ensembl
    9. oocyte maturation Source: RGD
    10. positive regulation of oocyte development Source: Ensembl
    11. positive regulation of vascular permeability Source: Ensembl
    12. regulation of meiosis Source: Ensembl
    13. response to cAMP Source: RGD
    14. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
    Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
    Short name:
    CGI-PDE A
    Gene namesi
    Name:Pde3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi61942. Pde3a.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase APRO_0000198801Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei310 – 3101PhosphoserineBy similarity
    Modified residuei520 – 5201PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ62865.

    Expressioni

    Gene expression databases

    GenevestigatoriQ62865.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000032282.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62865.
    SMRiQ62865. Positions 677-1087.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei127 – 14721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni728 – 1086359CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    GeneTreeiENSGT00750000117528.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ62865.
    KOiK13296.
    OMAiPETMMFL.
    OrthoDBiEOG7KDF95.
    PhylomeDBiQ62865.
    TreeFamiTF329631.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62865-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVRGEAAQD WAKPGLRGPS PAPVARGDHR CRGGSPSSPR GSGCCWRALA     50
    LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELES SQEAAAEEEE 100
    EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA 150
    GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW 200
    LVLRLRLGVL MVALTSALRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR 250
    YAEQLLPQCS GPAPPRERFG SQSSARTKEE IPGWKRRRRS SSVVAGEMSG 300
    CGGKSHRRTS LPCIPREQLM GHSEWDHKRG SRGSQSGTSV TVDIAVMGEA 350
    HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE 400
    NYTCSDSEEG LEKDKLAIPK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL 450
    EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APVLMTLTKS RSFTSSYAVS 500
    AANHVKAKKQ NRPGGLDKIS PVPSPSSSPP QGSPTSSPVS GIASVQFPES 550
    PEVTTKRGPG SHRALTYTQS APDLSPQIPP SPVICSSCGR PYSQGNPADG 600
    PSERSGPAMQ KPNRTDDTSQ VTSDYETNNN SDSSDILQND EEAECQREPL 650
    RKASACGTYT PQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLV 700
    ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP 750
    YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG 800
    YVFSKAYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA 850
    TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 900
    VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD 950
    INGPAKCKDL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL 1000
    QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH 1050
    EEETCENSEA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE 1100
    NQAPDQAPLQ HSSEQIQAIK EEEEEKGKPR AEETLAPQPD L 1141
    Length:1,141
    Mass (Da):124,301
    Last modified:November 1, 1996 - v1
    Checksum:iA333DFB44F6F33F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38179 mRNA. Translation: AAA84964.1.
    RefSeqiNP_059033.1. NM_017337.1.
    UniGeneiRn.44403.

    Genome annotation databases

    EnsembliENSRNOT00000032843; ENSRNOP00000032282; ENSRNOG00000025042.
    GeneIDi50678.
    KEGGirno:50678.
    UCSCiRGD:61942. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U38179 mRNA. Translation: AAA84964.1 .
    RefSeqi NP_059033.1. NM_017337.1.
    UniGenei Rn.44403.

    3D structure databases

    ProteinModelPortali Q62865.
    SMRi Q62865. Positions 677-1087.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000032282.

    Chemistry

    BindingDBi Q62865.

    Proteomic databases

    PRIDEi Q62865.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000032843 ; ENSRNOP00000032282 ; ENSRNOG00000025042 .
    GeneIDi 50678.
    KEGGi rno:50678.
    UCSCi RGD:61942. rat.

    Organism-specific databases

    CTDi 5139.
    RGDi 61942. Pde3a.

    Phylogenomic databases

    eggNOGi NOG145074.
    GeneTreei ENSGT00750000117528.
    HOGENOMi HOG000060144.
    HOVERGENi HBG053541.
    InParanoidi Q62865.
    KOi K13296.
    OMAi PETMMFL.
    OrthoDBi EOG7KDF95.
    PhylomeDBi Q62865.
    TreeFami TF329631.

    Miscellaneous databases

    NextBioi 610514.
    PROi Q62865.

    Gene expression databases

    Genevestigatori Q62865.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and characterization of deletion recombinants of two cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3)."
      He R., Komas N., Ekholm D., Murata T., Taira M., Hockman S.C., Degerman E., Manganiello V.C.
      Cell Biochem. Biophys. 29:89-111(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adipose tissue.

    Entry informationi

    Entry nameiPDE3A_RAT
    AccessioniPrimary (citable) accession number: Q62865
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3