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Protein

Proprotein convertase subtilisin/kexin type 7

Gene

Pcsk7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Likely to represent a ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif.

Catalytic activityi

Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Xaa-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.

Cofactori

Enzyme regulationi

Inhibited by zinc and copper.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei186 – 1861Charge relay systemBy similarity
Active sitei227 – 2271Charge relay systemBy similarity
Active sitei405 – 4051Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.077.

Names & Taxonomyi

Protein namesi
Recommended name:
Proprotein convertase subtilisin/kexin type 7 (EC:3.4.21.-)
Alternative name(s):
Prohormone convertase 7
Proprotein convertase 7
Short name:
PC7
Short name:
rPC7
Subtilisin/kexin-like protease PC7
Gene namesi
Name:Pcsk7
Synonyms:Pc7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3275. Pcsk7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini141 – 666526ExtracellularSequence analysisAdd
BLAST
Transmembranei667 – 68721HelicalSequence analysisAdd
BLAST
Topological domaini688 – 78396CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Add
BLAST
Propeptidei37 – 140104PRO_0000027118Add
BLAST
Chaini141 – 783643Proprotein convertase subtilisin/kexin type 7PRO_0000027119Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence analysis

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei140 – 1412Cleavage; by autolysis

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ62849.
PRIDEiQ62849.

PTM databases

PhosphoSiteiQ62849.

Miscellaneous databases

PMAP-CutDBQ62849.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in colon and spleen.1 Publication

Gene expression databases

GenevisibleiQ62849. RN.

Interactioni

Protein-protein interaction databases

DIPiDIP-29905N.
STRINGi10116.ENSRNOP00000023677.

Structurei

3D structure databases

ProteinModelPortaliQ62849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 473293Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ62849.
KOiK08673.
OMAiHEAVRWH.
OrthoDBiEOG73JKV3.
PhylomeDBiQ62849.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKGRQKVPR LDARLGLPIC LCLELAIFFL VPQVMGLTEA GGLDTLGAGG
60 70 80 90 100
LSWAVHLDSL EGERKEESLI QQANAVAQAA GLVNAGRIGE LQGHYLFVQP
110 120 130 140 150
AGHGQAMEAE AMRQQAEAVL AKHEAVRWHS EQRLLKRAKR SIHFNDPKYP
160 170 180 190 200
QQWHLNNRRS PGRDINVTGV WERNVTGRGV TVVVVDDGVE HTVQDIAPNY
210 220 230 240 250
SPEGSYDLNS NDPDPMPHPD EENGNHHGTR CAGEIAAVPN NSFCAVGVAY
260 270 280 290 300
GSRIAGIRVL DGPLTDSMEA VAFNKHYQIN DIYSCSWGPD DDGKTVDGPH
310 320 330 340 350
QLGKAALQHG VMAGRQGFGS IFVVASGNGG QHNDNCNYDG YANSIYTVTI
360 370 380 390 400
GAVDEEGRMP FYAEECASML AVTFSGGDKM LRSIVTTDWD LQKGTGCTEG
410 420 430 440 450
HTGTSAAAPL AAGMIALMLQ VRPCLTWRDV QHIIVFTATQ YEDHRADWLT
460 470 480 490 500
NEAGFSHSHQ HGFGLLNAWR LVNAAKIWTS VPYLASYVSP MLKENKAVPR
510 520 530 540 550
SPHSLEVLWN VSRTDLEMSG LKTLEHVAVT VSITHPRRGS LELKLFCPSG
560 570 580 590 600
MMSLIGAPRS MDSDPNGFND WTFSTVRCWG ERARGVYRLV IRDVGDEPLQ
610 620 630 640 650
VGILQQWQLT LYGSTWSPVD IKDRQSLLES AMSGKYLHDD FTLPCPPGLK
660 670 680 690 700
IPEEDGYSIT PNTLKTLVLV GCFSVFWTIY YMLEVCLSQR SKASTHGCRR
710 720 730 740 750
GCCPWPPQSQ NSKEVGTALE SMPLCSSKDL DGVDSEHGDC TTASSLLAPE
760 770 780
LLGEADWSLS QNSKSDLDCP PHQPPDLKDG QIC
Length:783
Mass (Da):85,599
Last modified:November 1, 1996 - v1
Checksum:i595E159819F5E653
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36580 mRNA. Translation: AAB39919.1.
PIRiJC6136.
RefSeqiNP_062119.1. NM_019246.1.
UniGeneiRn.10653.

Genome annotation databases

EnsembliENSRNOT00000023677; ENSRNOP00000023677; ENSRNOG00000017478.
GeneIDi29606.
KEGGirno:29606.
UCSCiRGD:3275. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36580 mRNA. Translation: AAB39919.1.
PIRiJC6136.
RefSeqiNP_062119.1. NM_019246.1.
UniGeneiRn.10653.

3D structure databases

ProteinModelPortaliQ62849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29905N.
STRINGi10116.ENSRNOP00000023677.

Protein family/group databases

MEROPSiS08.077.

PTM databases

PhosphoSiteiQ62849.

Proteomic databases

PaxDbiQ62849.
PRIDEiQ62849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023677; ENSRNOP00000023677; ENSRNOG00000017478.
GeneIDi29606.
KEGGirno:29606.
UCSCiRGD:3275. rat.

Organism-specific databases

CTDi9159.
RGDi3275. Pcsk7.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ62849.
KOiK08673.
OMAiHEAVRWH.
OrthoDBiEOG73JKV3.
PhylomeDBiQ62849.
TreeFamiTF314277.

Miscellaneous databases

NextBioi609782.
PMAP-CutDBQ62849.
PROiQ62849.

Gene expression databases

GenevisibleiQ62849. RN.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA structure, tissue distribution, and chromosomal localization of rat PC7, a novel mammalian proprotein convertase closest to yeast kexin-like proteinases."
    Seidah N.G., Hamelin J., Mamarbachi M., Dong W., Tadros H., Mbikay M., Chretien M., Day R.
    Proc. Natl. Acad. Sci. U.S.A. 93:3388-3393(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
    Tissue: Pheochromocytoma and Spleen.

Entry informationi

Entry nameiPCSK7_RAT
AccessioniPrimary (citable) accession number: Q62849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.