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Q62848 (ARFG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor GTPase-activating protein 1

Short name=ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name=ARF1 GAP
ARF1-directed GTPase-activating protein
Gene names
Name:Arfgap1
Synonyms:Arf1gap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Ref.1

Subunit structure

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity. Ref.1 Ref.3 Ref.5 Ref.6

Subcellular location

Cytoplasm. Golgi apparatus. Note: Associates with the Golgi complex.

Tissue specificity

Widely expressed. Highly expressed in brain and liver.

Domain

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes. Ref.7

Sequence similarities

Contains 1 Arf-GAP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LRRK2Q5S0077EBI-4398879,EBI-5323863From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q62848-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62848-2)

Also known as: W15;

The sequence of this isoform differs from the canonical sequence as follows:
     21-57: Missing.
Isoform 3 (identifier: Q62848-3)

Also known as: Z5;

The sequence of this isoform differs from the canonical sequence as follows:
     280-283: QGVG → GLPC
     284-415: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415ADP-ribosylation factor GTPase-activating protein 1
PRO_0000074192

Regions

Domain7 – 124118Arf-GAP
Zinc finger22 – 4524C4-type

Amino acid modifications

Modified residue1351Phosphothreonine By similarity
Modified residue1891Phosphothreonine By similarity
Modified residue2311N6-acetyllysine By similarity

Natural variations

Alternative sequence21 – 5737Missing in isoform 2.
VSP_000300
Alternative sequence280 – 2834QGVG → GLPC in isoform 3.
VSP_000301
Alternative sequence284 – 415132Missing in isoform 3.
VSP_000302

Experimental info

Mutagenesis221C → A: Loss of GAP activity. Ref.2 Ref.4
Mutagenesis251C → A: Loss of GAP activity. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 90015417E3E717BB

FASTA41545,442
        10         20         30         40         50         60 
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR 

        70         80         90        100        110        120 
SVTMDKWKDI ELEKMKAGGN AKFREFLEAQ DDYEPSWSLQ DKYSSRAAAL FRDKVATLAE 

       130        140        150        160        170        180 
GKEWSLESSP AQNWTPPQPK TLQFTAHRPA GQPQNVTTSG DKAFEDWLND DLGSYQGAQE 

       190        200        210        220        230        240 
NRYVGFGNTV PPQKREDDFL NSAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA 

       250        260        270        280        290        300 
SELGHSLNEN VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED 

       310        320        330        340        350        360 
TSDRPLEGHS YQNSSGDNSQ NSTIDQSFWE TFGSAEPPKA KSPSSDSWTC ADASTGRRSS 

       370        380        390        400        410 
DSWDIWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA APSTAADEGW DNQNW 

« Hide

Isoform 2 (W15) [UniParc].

Checksum: 897FCD20F2687D68
Show »

FASTA37841,335
Isoform 3 (Z5) [UniParc].

Checksum: 82D886C3174607FE
Show »

FASTA28331,280

References

[1]"ADP-ribosylation factor-directed GTPase-activating protein. Purification and partial characterization."
Makler V., Cukierman E., Rotman M., Admon A., Cassel D.
J. Biol. Chem. 270:5232-5237(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ARF1, PROTEIN SEQUENCE OF 82-95; 140-160; 183-191; 246-255; 264-278; 297-306 AND 361-375.
Tissue: Liver.
[2]"The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization."
Cukierman E., Huber I., Rotman M., Cassel D.
Science 270:1999-2002(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), CHARACTERIZATION, MUTAGENESIS OF CYS-22 AND CYS-25.
Tissue: Liver.
[3]"The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1."
Aoe T., Cukierman E., Lee A., Cassel D., Peters P.J., Hsu V.W.
EMBO J. 16:7305-7316(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KDELR1.
[4]"Requirement for both the amino-terminal catalytic domain and a noncatalytic domain for in vivo activity of ADP-ribosylation factor GTPase-activating protein."
Huber I., Cukierman E., Rotman M., Aoe T., Hsu V.W., Cassel D.
J. Biol. Chem. 273:24786-24791(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-22.
[5]"KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET."
Majoul I., Straub M., Hell S.W., Duden R., Soling H.D.
Dev. Cell 1:139-153(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KDELR1 AND TMED2.
[6]"Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1."
Lanoix J., Ouwendijk J., Stark A., Szafer E., Cassel D., Dejgaard K., Weiss M., Nilsson T.
J. Cell Biol. 155:1199-1212(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNP24.
[7]"Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis."
Goldberg J.
Cell 96:893-902(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF CATALYTIC DOMAIN IN COMPLEX WITH HUMAN ARF1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35776 mRNA. Translation: AAC52337.1.
RefSeqNP_659558.1. NM_145090.3. [Q62848-1]
UniGeneRn.11219.

3D structure databases

ProteinModelPortalQ62848.
SMRQ62848. Positions 3-120.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid251587. 1 interaction.
IntActQ62848. 5 interactions.
MINTMINT-266301.

Chemistry

ChEMBLCHEMBL2146307.

PTM databases

PhosphoSiteQ62848.

Proteomic databases

PRIDEQ62848.

Protocols and materials databases

DNASU246310.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013900; ENSRNOP00000013900; ENSRNOG00000043150. [Q62848-1]
ENSRNOT00000055038; ENSRNOP00000051916; ENSRNOG00000043150. [Q62848-2]
GeneID246310.
KEGGrno:246310.

Organism-specific databases

CTD55738.
RGD708452. Arfgap1.

Phylogenomic databases

eggNOGCOG5347.
GeneTreeENSGT00670000098104.
HOGENOMHOG000008494.
HOVERGENHBG050562.
KOK12492.
OMASDSWENW.
OrthoDBEOG7C8GH3.
PhylomeDBQ62848.

Gene expression databases

GenevestigatorQ62848.

Family and domain databases

InterProIPR001164. ArfGAP.
[Graphical view]
PfamPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623732.
PROQ62848.

Entry information

Entry nameARFG1_RAT
AccessionPrimary (citable) accession number: Q62848
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families