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Q62848

- ARFG1_RAT

UniProt

Q62848 - ARFG1_RAT

Protein

ADP-ribosylation factor GTPase-activating protein 1

Gene

Arfgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. GTPase activator activity Source: RGD
    3. protein binding Source: IntAct
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. regulation of ARF GTPase activity Source: InterPro
    3. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_93415. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor GTPase-activating protein 1
    Short name:
    ARF GAP 1
    Alternative name(s):
    ADP-ribosylation factor 1 GTPase-activating protein
    Short name:
    ARF1 GAP
    ARF1-directed GTPase-activating protein
    Gene namesi
    Name:Arfgap1
    Synonyms:Arf1gap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi708452. Arfgap1.

    Subcellular locationi

    Cytoplasm. Golgi apparatus
    Note: Associates with the Golgi complex.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: Ensembl
    2. Golgi membrane Source: RGD
    3. nuclear membrane Source: Ensembl
    4. plasma membrane Source: Ensembl
    5. synapse Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi22 – 221C → A: Loss of GAP activity. 2 Publications
    Mutagenesisi25 – 251C → A: Loss of GAP activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415ADP-ribosylation factor GTPase-activating protein 1PRO_0000074192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei135 – 1351PhosphothreonineBy similarity
    Modified residuei189 – 1891PhosphothreonineBy similarity
    Modified residuei231 – 2311N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ62848.

    PTM databases

    PhosphoSiteiQ62848.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in brain and liver.

    Gene expression databases

    GenevestigatoriQ62848.

    Interactioni

    Subunit structurei

    Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LRRK2Q5S0077EBI-4398879,EBI-5323863From a different organism.

    Protein-protein interaction databases

    BioGridi251587. 1 interaction.
    IntActiQ62848. 5 interactions.
    MINTiMINT-266301.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62848.
    SMRiQ62848. Positions 3-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 124118Arf-GAPPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.

    Sequence similaritiesi

    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    GeneTreeiENSGT00670000098104.
    HOGENOMiHOG000008494.
    HOVERGENiHBG050562.
    KOiK12492.
    OMAiSDSWENW.
    OrthoDBiEOG7C8GH3.
    PhylomeDBiQ62848.

    Family and domain databases

    InterProiIPR001164. ArfGAP.
    [Graphical view]
    PfamiPF01412. ArfGap. 1 hit.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00105. ArfGap. 1 hit.
    [Graphical view]
    PROSITEiPS50115. ARFGAP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Experimental confirmation may be lacking for some isoforms.

    Isoform 1 (identifier: Q62848-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR    50
    GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLEAQ DDYEPSWSLQ 100
    DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRPA 150
    GQPQNVTTSG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL 200
    NSAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN 250
    VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED 300
    TSDRPLEGHS YQNSSGDNSQ NSTIDQSFWE TFGSAEPPKA KSPSSDSWTC 350
    ADASTGRRSS DSWDIWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA 400
    APSTAADEGW DNQNW 415
    Length:415
    Mass (Da):45,442
    Last modified:November 1, 1996 - v1
    Checksum:i90015417E3E717BB
    GO
    Isoform 2 (identifier: Q62848-2) [UniParc]FASTAAdd to Basket

    Also known as: W15

    The sequence of this isoform differs from the canonical sequence as follows:
         21-57: Missing.

    Show »
    Length:378
    Mass (Da):41,335
    Checksum:i897FCD20F2687D68
    GO
    Isoform 3 (identifier: Q62848-3) [UniParc]FASTAAdd to Basket

    Also known as: Z5

    The sequence of this isoform differs from the canonical sequence as follows:
         280-283: QGVG → GLPC
         284-415: Missing.

    Show »
    Length:283
    Mass (Da):31,280
    Checksum:i82D886C3174607FE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei21 – 5737Missing in isoform 2. 1 PublicationVSP_000300Add
    BLAST
    Alternative sequencei280 – 2834QGVG → GLPC in isoform 3. 1 PublicationVSP_000301
    Alternative sequencei284 – 415132Missing in isoform 3. 1 PublicationVSP_000302Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35776 mRNA. Translation: AAC52337.1.
    RefSeqiNP_659558.1. NM_145090.3. [Q62848-1]
    UniGeneiRn.11219.

    Genome annotation databases

    EnsembliENSRNOT00000013900; ENSRNOP00000013900; ENSRNOG00000043150. [Q62848-1]
    ENSRNOT00000055038; ENSRNOP00000051916; ENSRNOG00000043150. [Q62848-2]
    GeneIDi246310.
    KEGGirno:246310.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35776 mRNA. Translation: AAC52337.1 .
    RefSeqi NP_659558.1. NM_145090.3. [Q62848-1 ]
    UniGenei Rn.11219.

    3D structure databases

    ProteinModelPortali Q62848.
    SMRi Q62848. Positions 3-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251587. 1 interaction.
    IntActi Q62848. 5 interactions.
    MINTi MINT-266301.

    Chemistry

    ChEMBLi CHEMBL2146307.

    PTM databases

    PhosphoSitei Q62848.

    Proteomic databases

    PRIDEi Q62848.

    Protocols and materials databases

    DNASUi 246310.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013900 ; ENSRNOP00000013900 ; ENSRNOG00000043150 . [Q62848-1 ]
    ENSRNOT00000055038 ; ENSRNOP00000051916 ; ENSRNOG00000043150 . [Q62848-2 ]
    GeneIDi 246310.
    KEGGi rno:246310.

    Organism-specific databases

    CTDi 55738.
    RGDi 708452. Arfgap1.

    Phylogenomic databases

    eggNOGi COG5347.
    GeneTreei ENSGT00670000098104.
    HOGENOMi HOG000008494.
    HOVERGENi HBG050562.
    KOi K12492.
    OMAi SDSWENW.
    OrthoDBi EOG7C8GH3.
    PhylomeDBi Q62848.

    Enzyme and pathway databases

    Reactomei REACT_93415. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 623732.
    PROi Q62848.

    Gene expression databases

    Genevestigatori Q62848.

    Family and domain databases

    InterProi IPR001164. ArfGAP.
    [Graphical view ]
    Pfami PF01412. ArfGap. 1 hit.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00105. ArfGap. 1 hit.
    [Graphical view ]
    PROSITEi PS50115. ARFGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ADP-ribosylation factor-directed GTPase-activating protein. Purification and partial characterization."
      Makler V., Cukierman E., Rotman M., Admon A., Cassel D.
      J. Biol. Chem. 270:5232-5237(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ARF1, PROTEIN SEQUENCE OF 82-95; 140-160; 183-191; 246-255; 264-278; 297-306 AND 361-375.
      Tissue: Liver.
    2. "The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization."
      Cukierman E., Huber I., Rotman M., Cassel D.
      Science 270:1999-2002(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), CHARACTERIZATION, MUTAGENESIS OF CYS-22 AND CYS-25.
      Tissue: Liver.
    3. "The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1."
      Aoe T., Cukierman E., Lee A., Cassel D., Peters P.J., Hsu V.W.
      EMBO J. 16:7305-7316(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KDELR1.
    4. "Requirement for both the amino-terminal catalytic domain and a noncatalytic domain for in vivo activity of ADP-ribosylation factor GTPase-activating protein."
      Huber I., Cukierman E., Rotman M., Aoe T., Hsu V.W., Cassel D.
      J. Biol. Chem. 273:24786-24791(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-22.
    5. "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET."
      Majoul I., Straub M., Hell S.W., Duden R., Soling H.D.
      Dev. Cell 1:139-153(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KDELR1 AND TMED2.
    6. "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1."
      Lanoix J., Ouwendijk J., Stark A., Szafer E., Cassel D., Dejgaard K., Weiss M., Nilsson T.
      J. Cell Biol. 155:1199-1212(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNP24.
    7. "Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis."
      Goldberg J.
      Cell 96:893-902(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF CATALYTIC DOMAIN IN COMPLEX WITH HUMAN ARF1.

    Entry informationi

    Entry nameiARFG1_RAT
    AccessioniPrimary (citable) accession number: Q62848
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3