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Protein

ADP-ribosylation factor GTPase-activating protein 1

Gene

Arfgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. GTPase activator activity Source: RGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of GTPase activity Source: GOC
  2. protein transport Source: UniProtKB-KW
  3. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_287881. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 1
Short name:
ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name:
ARF1 GAP
ARF1-directed GTPase-activating protein
Gene namesi
Name:Arfgap1
Synonyms:Arf1gap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi708452. Arfgap1.

Subcellular locationi

  1. Cytoplasm
  2. Golgi apparatus

  3. Note: Associates with the Golgi complex.

GO - Cellular componenti

  1. Golgi membrane Source: RGD
  2. synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi22 – 221C → A: Loss of GAP activity. 2 Publications
Mutagenesisi25 – 251C → A: Loss of GAP activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415ADP-ribosylation factor GTPase-activating protein 1PRO_0000074192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphothreonineBy similarity
Modified residuei231 – 2311N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ62848.

PTM databases

PhosphoSiteiQ62848.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in brain and liver.

Gene expression databases

ExpressionAtlasiQ62848. baseline.
GenevestigatoriQ62848.

Interactioni

Subunit structurei

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0077EBI-4398879,EBI-5323863From a different organism.

Protein-protein interaction databases

BioGridi251587. 1 interaction.
IntActiQ62848. 5 interactions.
MINTiMINT-266301.

Structurei

3D structure databases

ProteinModelPortaliQ62848.
SMRiQ62848. Positions 3-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 124118Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Domaini

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00670000098104.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ62848.
KOiK12492.
OMAiDSWDIWG.
OrthoDBiEOG7C8GH3.
PhylomeDBiQ62848.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q62848-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR
60 70 80 90 100
GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLEAQ DDYEPSWSLQ
110 120 130 140 150
DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRPA
160 170 180 190 200
GQPQNVTTSG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL
210 220 230 240 250
NSAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN
260 270 280 290 300
VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED
310 320 330 340 350
TSDRPLEGHS YQNSSGDNSQ NSTIDQSFWE TFGSAEPPKA KSPSSDSWTC
360 370 380 390 400
ADASTGRRSS DSWDIWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA
410
APSTAADEGW DNQNW
Length:415
Mass (Da):45,442
Last modified:November 1, 1996 - v1
Checksum:i90015417E3E717BB
GO
Isoform 2 (identifier: Q62848-2) [UniParc]FASTAAdd to basket

Also known as: W15

The sequence of this isoform differs from the canonical sequence as follows:
     21-57: Missing.

Show »
Length:378
Mass (Da):41,335
Checksum:i897FCD20F2687D68
GO
Isoform 3 (identifier: Q62848-3) [UniParc]FASTAAdd to basket

Also known as: Z5

The sequence of this isoform differs from the canonical sequence as follows:
     280-283: QGVG → GLPC
     284-415: Missing.

Show »
Length:283
Mass (Da):31,280
Checksum:i82D886C3174607FE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei21 – 5737Missing in isoform 2. 1 PublicationVSP_000300Add
BLAST
Alternative sequencei280 – 2834QGVG → GLPC in isoform 3. 1 PublicationVSP_000301
Alternative sequencei284 – 415132Missing in isoform 3. 1 PublicationVSP_000302Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35776 mRNA. Translation: AAC52337.1.
RefSeqiNP_659558.1. NM_145090.3. [Q62848-1]
UniGeneiRn.11219.

Genome annotation databases

EnsembliENSRNOT00000013900; ENSRNOP00000013900; ENSRNOG00000043150. [Q62848-1]
ENSRNOT00000055038; ENSRNOP00000051916; ENSRNOG00000043150. [Q62848-2]
GeneIDi246310.
KEGGirno:246310.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35776 mRNA. Translation: AAC52337.1.
RefSeqiNP_659558.1. NM_145090.3. [Q62848-1]
UniGeneiRn.11219.

3D structure databases

ProteinModelPortaliQ62848.
SMRiQ62848. Positions 3-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251587. 1 interaction.
IntActiQ62848. 5 interactions.
MINTiMINT-266301.

Chemistry

BindingDBiQ62848.
ChEMBLiCHEMBL2146307.

PTM databases

PhosphoSiteiQ62848.

Proteomic databases

PRIDEiQ62848.

Protocols and materials databases

DNASUi246310.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013900; ENSRNOP00000013900; ENSRNOG00000043150. [Q62848-1]
ENSRNOT00000055038; ENSRNOP00000051916; ENSRNOG00000043150. [Q62848-2]
GeneIDi246310.
KEGGirno:246310.

Organism-specific databases

CTDi55738.
RGDi708452. Arfgap1.

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00670000098104.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ62848.
KOiK12492.
OMAiDSWDIWG.
OrthoDBiEOG7C8GH3.
PhylomeDBiQ62848.

Enzyme and pathway databases

ReactomeiREACT_287881. COPI Mediated Transport.

Miscellaneous databases

NextBioi623732.
PROiQ62848.

Gene expression databases

ExpressionAtlasiQ62848. baseline.
GenevestigatoriQ62848.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "ADP-ribosylation factor-directed GTPase-activating protein. Purification and partial characterization."
    Makler V., Cukierman E., Rotman M., Admon A., Cassel D.
    J. Biol. Chem. 270:5232-5237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ARF1, PROTEIN SEQUENCE OF 82-95; 140-160; 183-191; 246-255; 264-278; 297-306 AND 361-375.
    Tissue: Liver.
  2. "The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization."
    Cukierman E., Huber I., Rotman M., Cassel D.
    Science 270:1999-2002(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), CHARACTERIZATION, MUTAGENESIS OF CYS-22 AND CYS-25.
    Tissue: Liver.
  3. "The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1."
    Aoe T., Cukierman E., Lee A., Cassel D., Peters P.J., Hsu V.W.
    EMBO J. 16:7305-7316(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KDELR1.
  4. "Requirement for both the amino-terminal catalytic domain and a noncatalytic domain for in vivo activity of ADP-ribosylation factor GTPase-activating protein."
    Huber I., Cukierman E., Rotman M., Aoe T., Hsu V.W., Cassel D.
    J. Biol. Chem. 273:24786-24791(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-22.
  5. "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET."
    Majoul I., Straub M., Hell S.W., Duden R., Soling H.D.
    Dev. Cell 1:139-153(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KDELR1 AND TMED2.
  6. "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1."
    Lanoix J., Ouwendijk J., Stark A., Szafer E., Cassel D., Dejgaard K., Weiss M., Nilsson T.
    J. Cell Biol. 155:1199-1212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNP24.
  7. "Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis."
    Goldberg J.
    Cell 96:893-902(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF CATALYTIC DOMAIN IN COMPLEX WITH HUMAN ARF1.

Entry informationi

Entry nameiARFG1_RAT
AccessioniPrimary (citable) accession number: Q62848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.