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Protein

ADP-ribosylation factor GTPase-activating protein 1

Gene

Arfgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 45C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processER-Golgi transport, Protein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 1
Short name:
ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name:
ARF1 GAP
ARF1-directed GTPase-activating protein
Gene namesi
Name:Arfgap1
Synonyms:Arf1gap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi708452. Arfgap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22C → A: Loss of GAP activity. 2 Publications1
Mutagenesisi25C → A: Loss of GAP activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2146307.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741921 – 415ADP-ribosylation factor GTPase-activating protein 1Add BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphothreonineCombined sources1
Modified residuei189PhosphothreonineBy similarity1
Modified residuei231N6-acetyllysineBy similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei342PhosphoserineBy similarity1
Modified residuei345PhosphoserineBy similarity1
Modified residuei347PhosphoserineCombined sources1
Modified residuei349PhosphothreonineBy similarity1
Modified residuei360PhosphoserineCombined sources1
Modified residuei362PhosphoserineBy similarity1
Modified residuei379PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62848.
PRIDEiQ62848.

PTM databases

iPTMnetiQ62848.
PhosphoSitePlusiQ62848.
SwissPalmiQ62848.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in brain and liver.

Gene expression databases

BgeeiENSRNOG00000043150.
ExpressionAtlasiQ62848. baseline.
GenevisibleiQ62848. RN.

Interactioni

Subunit structurei

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0077EBI-4398879,EBI-5323863From Homo sapiens.

Protein-protein interaction databases

BioGridi251587. 11 interactors.
IntActiQ62848. 5 interactors.
MINTiMINT-266301.
STRINGi10116.ENSRNOP00000013900.

Chemistry databases

BindingDBiQ62848.

Structurei

3D structure databases

ProteinModelPortaliQ62848.
SMRiQ62848.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST118

Domaini

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 45C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0704. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00890000139515.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ62848.
KOiK12492.
OrthoDBiEOG091G0DQ3.
PhylomeDBiQ62848.

Family and domain databases

InterProiView protein in InterPro
IPR001164. ArfGAP.
PfamiView protein in Pfam
PF01412. ArfGap. 1 hit.
PRINTSiPR00405. REVINTRACTNG.
SMARTiView protein in SMART
SM00105. ArfGap. 1 hit.
PROSITEiView protein in PROSITE
PS50115. ARFGAP. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q62848-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR
60 70 80 90 100
GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLEAQ DDYEPSWSLQ
110 120 130 140 150
DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRPA
160 170 180 190 200
GQPQNVTTSG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL
210 220 230 240 250
NSAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN
260 270 280 290 300
VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED
310 320 330 340 350
TSDRPLEGHS YQNSSGDNSQ NSTIDQSFWE TFGSAEPPKA KSPSSDSWTC
360 370 380 390 400
ADASTGRRSS DSWDIWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA
410
APSTAADEGW DNQNW
Length:415
Mass (Da):45,442
Last modified:November 1, 1996 - v1
Checksum:i90015417E3E717BB
GO
Isoform 2 (identifier: Q62848-2) [UniParc]FASTAAdd to basket
Also known as: W15

The sequence of this isoform differs from the canonical sequence as follows:
     21-57: Missing.

Show »
Length:378
Mass (Da):41,335
Checksum:i897FCD20F2687D68
GO
Isoform 3 (identifier: Q62848-3) [UniParc]FASTAAdd to basket
Also known as: Z5

The sequence of this isoform differs from the canonical sequence as follows:
     280-283: QGVG → GLPC
     284-415: Missing.

Show »
Length:283
Mass (Da):31,280
Checksum:i82D886C3174607FE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00030021 – 57Missing in isoform 2. 1 PublicationAdd BLAST37
Alternative sequenceiVSP_000301280 – 283QGVG → GLPC in isoform 3. 1 Publication4
Alternative sequenceiVSP_000302284 – 415Missing in isoform 3. 1 PublicationAdd BLAST132

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35776 mRNA. Translation: AAC52337.1.
RefSeqiNP_659558.1. NM_145090.3. [Q62848-1]
XP_017446953.1. XM_017591464.1. [Q62848-1]
UniGeneiRn.11219.

Genome annotation databases

EnsembliENSRNOT00000013900; ENSRNOP00000013900; ENSRNOG00000043150. [Q62848-1]
GeneIDi246310.
KEGGirno:246310.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARFG1_RAT
AccessioniPrimary (citable) accession number: Q62848
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome