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Protein

Gamma-adducin

Gene

Add3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

GO - Molecular functioni

  • protein kinase C binding Source: RGD

GO - Biological processi

  • response to drug Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-adducin
Alternative name(s):
Adducin-like protein 70
Protein kinase C-binding protein 35H
Gene namesi
Name:Add3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2043. Add3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 705704Gamma-adducinPRO_0000218538Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei42 – 421PhosphoserineBy similarity
Modified residuei59 – 591PhosphothreonineBy similarity
Modified residuei64 – 641PhosphoserineBy similarity
Modified residuei402 – 4021PhosphoserineBy similarity
Modified residuei414 – 4141PhosphoserineBy similarity
Modified residuei423 – 4231PhosphoserineCombined sources
Modified residuei442 – 4421PhosphoserineBy similarity
Modified residuei461 – 4611PhosphoserineBy similarity
Modified residuei583 – 5831PhosphoserineCombined sources
Modified residuei585 – 5851PhosphoserineCombined sources
Modified residuei590 – 5901PhosphoserineBy similarity
Modified residuei592 – 5921PhosphoserineBy similarity
Modified residuei598 – 5981PhosphothreonineBy similarity
Modified residuei600 – 6001PhosphoserineBy similarity
Modified residuei672 – 6721PhosphoserineCombined sources
Modified residuei676 – 6761PhosphoserineCombined sources
Modified residuei678 – 6781PhosphoserineBy similarity
Modified residuei680 – 6801PhosphoserineCombined sources
Modified residuei682 – 6821Phosphoserine; by PKCBy similarity
Modified residuei692 – 6921PhosphoserineBy similarity

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62847.
PRIDEiQ62847.

PTM databases

iPTMnetiQ62847.
PhosphoSiteiQ62847.

Interactioni

Subunit structurei

Heterodimer of an alpha and a gamma subunit.1 Publication

GO - Molecular functioni

  • protein kinase C binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043399.

Structurei

3D structure databases

ProteinModelPortaliQ62847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni683 – 70018Interaction with calmodulinSequence analysisAdd
BLAST

Domaini

Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ62847.
KOiK18622.
PhylomeDBiQ62847.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027772. ADD3.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF5. PTHR10672:SF5. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 2 (identifier: Q62847-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSDSSQAVI TTPPPPSMPH KERYFDRINE SDPEYLRERN MSPDLREDFN
60 70 80 90 100
MMEQRKRVTQ ILQSPAFRED LECLTQEQMK KGHDPTGLLA LQQIADYIMA
110 120 130 140 150
NSFTGFSSPP LSLGMVTPIN DLPGADTSSY VKGEKLTRCE LASLYRLADL
160 170 180 190 200
FGWAHLANTY ISVRVSKEQD HIIIIPRGLP FSEATASALV KVNIIGEVVD
210 220 230 240 250
QGSTNLKIDH SGFSPHAAIY STRPDVKCVI HIHTLATAAV SSMKCGILPI
260 270 280 290 300
SQESLILGDV AYYDYQGSLD EEEERIELQK VLGPSCKVLV LRNHGVVALG
310 320 330 340 350
ETLEEAFHYI FNVQMACEIQ VQAVAGAGGV DNLLILDLQK YKAFTHGVAM
360 370 380 390 400
TGGGGVNMGS HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLVREKPRH
410 420 430 440 450
KSDVEIPATV TAFSFEDDSV PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN
460 470 480 490 500
VPEESRNGET SPRTKITWMK AEDPSKVSSG TPIKIEDPNQ FVPLNTNPTE
510 520 530 540 550
VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMRF EDDDQGPPAP
560 570 580 590 600
PNPFSHLMEG ELEEYTKTIE RQQQGLDDAE QESLSDDAAS VSQIQSQTQS
610 620 630 640 650
PQSVPERLEE NHELFSKSFT SVDVPVIVNG KDEMHDVEDE LAQRVSRLTT
660 670 680 690 700
STTIENIEIT IKSPDRTEEV LSPDGSPSKS PSKKKKKFRT PSFLKKNKKK

EKVEA
Length:705
Mass (Da):78,804
Last modified:December 1, 2000 - v2
Checksum:iE8448431AD2E436B
GO
Isoform 1 (identifier: Q62847-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     578-609: Missing.

Show »
Length:673
Mass (Da):75,361
Checksum:i41A81150B27873F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti701 – 7055EKVEA → GES in AAC52277 (PubMed:7592723).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti572 – 5721Q → K in strain: Milan hypersensitive.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei578 – 60932Missing in isoform 1. 1 PublicationVSP_000190Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35775 mRNA. Translation: AAC52277.1.
RefSeqiNP_001157575.1. NM_001164103.1.
NP_113740.2. NM_031552.2.
UniGeneiRn.76589.

Genome annotation databases

GeneIDi25230.
KEGGirno:25230.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35775 mRNA. Translation: AAC52277.1.
RefSeqiNP_001157575.1. NM_001164103.1.
NP_113740.2. NM_031552.2.
UniGeneiRn.76589.

3D structure databases

ProteinModelPortaliQ62847.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043399.

PTM databases

iPTMnetiQ62847.
PhosphoSiteiQ62847.

Proteomic databases

PaxDbiQ62847.
PRIDEiQ62847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25230.
KEGGirno:25230.

Organism-specific databases

CTDi120.
RGDi2043. Add3.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ62847.
KOiK18622.
PhylomeDBiQ62847.

Miscellaneous databases

PROiQ62847.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027772. ADD3.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF5. PTHR10672:SF5. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "35H, a sequence isolated as a protein kinase C binding protein, is a novel member of the adducin family."
    Dong L., Chapline C., Mousseau B., Fowler L., Ramsay K., Stevens J.L., Jaken S.
    J. Biol. Chem. 270:25534-25540(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, INTERACTION WITH ADD1.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "Polymorphism of gamma-adducin gene in genetic hypertension and mapping of the gene to rat chromosome 1q55."
    Tripodi G., Szpirer C., Reina C., Szpirer J., Bianchi G.
    Biochem. Biophys. Res. Commun. 237:685-689(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Milan.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-583; SER-585; SER-672; SER-676 AND SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiADDG_RAT
AccessioniPrimary (citable) accession number: Q62847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.