Reviewed, UniProtKB/Swiss-Prot Q62844 (FYN_RAT)

Names and origin

Protein names

Recommended name:
    Proto-oncogene tyrosine-protein kinase Fyn
    EC=2.7.10.2
Alternative name(s):
    Proto-oncogene c-Fyn
    p59-Fyn

Gene names

Name:

Fyn

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Ref.3 UniProtKB P06241 UniProtKB P39688
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.3
Cofactor	Manganese. Ref.3
Enzyme regulation	Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site By similarity.
Subunit structure	Associates through its SH3 domain, to the p85 subunit of phosphatidylinositol 3-kinase. Interacts with the FYN-binding protein (FYB). Interacts with phosphorylated TOM1L1. Interacts with PAG1. Interacts with CD79A upon activation of the B-cell antigen receptor which increases FYN activity By similarity. Interacts with SH2D1A and SLAMF1. Interacts (via SH3 domain) with PRMT8 By similarity.
Subcellular location	Cell membrane. Note: Present and active in lipid rafts By similarity. Present in cell body and along the process of mature and developing oligodendroyctes. Ref.3 UniProtKB P39688
Developmental stage	Up-regulated during oligodendrocyte differentiation. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	ionotropic glutamate receptor signaling pathway Traceable author statement. Source: RGD peptidyl-tyrosine phosphorylation Inferred from mutant phenotype. Source: RGD
Cellular component	mitochondrial envelope Inferred from expression pattern. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity UniProtKB P06241
	Chain	2 – 537	536	Proto-oncogene tyrosine-protein kinase Fyn UniProtKB P06241		PRO_0000282948
Regions
	Domain	82 – 143	62	SH3
	Domain	149 – 246	98	SH2
	Domain	271 – 524	254	Protein kinase
	Nucleotide binding	277 – 285	9	ATP By similarity UniProtKB P28523
Sites
	Active site	390	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	299	1	ATP Ref.3
Amino acid modifications
	Modified residue	15	1	Phosphothreonine By similarity
	Modified residue	21	1	Phosphoserine By similarity UniProtKB P06241
	Modified residue	25	1	Phosphoserine By similarity
	Modified residue	185	1	Phosphotyrosine By similarity
	Modified residue	213	1	Phosphotyrosine By similarity
	Modified residue	214	1	Phosphotyrosine By similarity
	Modified residue	254	1	Phosphothreonine By similarity
	Modified residue	257	1	Phosphoserine By similarity
	Modified residue	420	1	Phosphotyrosine; by autocatalysis By similarity UniProtKB P06241
	Modified residue	440	1	Phosphotyrosine By similarity
	Modified residue	512	1	Phosphothreonine By similarity
	Modified residue	531	1	Phosphotyrosine By similarity UniProtKB P39688
	Lipidation	2	1	N-myristoyl glycine By similarity UniProtKB P39688
	Lipidation	3	1	S-palmitoyl cysteine By similarity UniProtKB P39688
	Lipidation	6	1	S-palmitoyl cysteine By similarity UniProtKB P39688
Experimental info
	Mutagenesis	299	1	K → M: Loss of kinase activity and reduction in oligodendrocyte process extension and myelin membrane formation. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q62844-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 11AE4420919DBF1C	FASTA	537	60,702
	10 20 30 40 50 60 MGCVQCKDKE AAKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY NNFHAAGGQG 70 80 90 100 110 120 LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFHKGEKFQ ILNSSEGDWW 130 140 150 160 170 180 EARSLTTGET GYIPSNYVAP VDSIQAEEWY FGKLGRKDAE RQLLSFGNPR GTFLIRESET 190 200 210 220 230 240 TKGAYSLSIR DWDDMKGDHV KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC 250 260 270 280 290 300 RLVVPCHKGM PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT 310 320 330 340 350 360 LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR 370 380 390 400 410 420 ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN GLICKIADFG LARLIEDNEY 430 440 450 460 470 480 TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE 490 500 510 520 530 RGYRMPCPQD CPISLHELMI HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL

References

[1]	"Expression of three type mRNAs encoding rat FYN by alternative splicing within 5' untranslated region." Nemoto K., Sekimoto M., Kageyama H., Fukamachi K., Nemoto F., Ueyama T., Senba E., Tomita I. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SHRSP. Tissue: Brain.
[2]	"Novel putative protein kinase clones from a rat large granular lymphocyte tumor cell line." Yue C.C. Mol. Immunol. 28:399-408(1991) [PubMed: 2062320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Morphological differentiation of oligodendrocytes requires activation of Fyn tyrosine kinase." Osterhout D.J., Wolven A., Wolf R.M., Resh M.D., Chao M.V. J. Cell Biol. 145:1209-1218(1999) [PubMed: 10366594] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-299.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35365 mRNA. Translation: AAA82942.1.
IPI	IPI00209210.
PIR	PT0199.
RefSeq	NP_036887.1.
UniGene	Rn.19361
3D structure databases
HSSP	HSSP built from PDB template 1FYN based on UniProtKB P06241.
SMR	Q62844. Positions 81-142, 84-537.
ModBase	Search...
Protein-protein interaction databases
STRING	Q62844.
PTM databases
PhosphoSite	Q62844.
Genome annotation databases
Ensembl	ENSRNOT00000000733; ENSRNOP00000000733; ENSRNOG00000000596; Rattus norvegicus. [Genome view]
GeneID	25150.
KEGG	rno:25150.
Organism-specific databases
CTD	25150.
RGD	2641. Fyn.
Phylogenomic databases
eggNOG	maNOG10466.
HOVERGEN	Q62844.
InParanoid	Q62844.
OMA	TTIPNYN.
OrthoDB	EOG9PP12J.
PhylomeDB	Q62844.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248.
Gene expression databases
ArrayExpress	Q62844.
Genevestigator	Q62844.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020745. Tyr_kinase_non-rcpt_Fyn. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF256. Tyr_kinase_non-rcpt_Fyn. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605589.

Entry information

Entry name

FYN_RAT

Accession

Primary (citable) accession number: Q62844

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)