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Q62844

- FYN_RAT

UniProt

Q62844 - FYN_RAT

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Protein

Tyrosine-protein kinase Fyn

Gene

Fyn

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1 By similarity. Interacts with UNC119 By similarity.By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationPROSITE-ProRule annotation

Cofactori

Manganese.1 Publication

Enzyme regulationi

Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei299 – 2991ATP1 PublicationPROSITE-ProRule annotation
Active sitei390 – 3901Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi277 – 2859ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. CD4 receptor binding Source: RGD
  3. CD8 receptor binding Source: RGD
  4. glycoprotein binding Source: RGD
  5. metal ion binding Source: UniProtKB-KW
  6. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  7. peptide hormone receptor binding Source: RGD
  8. phosphatidylinositol 3-kinase binding Source: RGD
  9. protein complex binding Source: RGD
  10. protein kinase activity Source: RGD
  11. protein tyrosine kinase activity Source: RGD
  12. receptor binding Source: RGD
  13. T cell receptor binding Source: RGD

GO - Biological processi

  1. activated T cell proliferation Source: Ensembl
  2. cellular response to growth factor stimulus Source: RGD
  3. cellular response to peptide hormone stimulus Source: RGD
  4. dendrite morphogenesis Source: Ensembl
  5. detection of mechanical stimulus involved in sensory perception of pain Source: Ensembl
  6. forebrain development Source: Ensembl
  7. ionotropic glutamate receptor signaling pathway Source: RGD
  8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
  9. negative regulation of gene expression Source: Ensembl
  10. negative regulation of neuron apoptotic process Source: UniProtKB
  11. negative regulation of protein catabolic process Source: Ensembl
  12. neuron migration Source: Ensembl
  13. peptidyl-tyrosine phosphorylation Source: RGD
  14. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  15. positive regulation of neuron projection development Source: Ensembl
  16. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
  17. positive regulation of protein localization to nucleus Source: Ensembl
  18. protein autophosphorylation Source: RGD
  19. regulation of cell shape Source: Ensembl
  20. response to drug Source: RGD
  21. response to ethanol Source: Ensembl
  22. T cell receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_195025. Signaling by SCF-KIT.
REACT_196225. Regulation of signaling by CBL.
REACT_197451. Regulation of KIT signaling.
REACT_197531. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_198646. FCGR activation.
REACT_198729. Constitutive PI3K/AKT Signaling in Cancer.
REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_212418. DAP12 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Fyn (EC:2.7.10.2)
Alternative name(s):
Proto-oncogene c-Fyn
p59-Fyn
Gene namesi
Name:FynImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi2641. Fyn.

Subcellular locationi

Cell membrane By similarity
Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking By similarity.By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome Source: Ensembl
  3. intracellular membrane-bounded organelle Source: RGD
  4. membrane Source: RGD
  5. membrane raft Source: Ensembl
  6. mitochondrion Source: RGD
  7. plasma membrane Source: UniProtKB-KW
  8. postsynaptic density Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi299 – 2991K → M: Loss of kinase activity and reduction in oligodendrocyte process extension and myelin membrane formation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 537536Tyrosine-protein kinase FynPRO_0000282948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Lipidationi6 – 61S-palmitoyl cysteineBy similarity
Modified residuei15 – 151PhosphothreonineBy similarity
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity
Modified residuei213 – 2131PhosphotyrosineBy similarity
Modified residuei214 – 2141PhosphotyrosineBy similarity
Modified residuei257 – 2571PhosphoserineBy similarity
Modified residuei420 – 4201Phosphotyrosine; by autocatalysisBy similarity
Modified residuei440 – 4401PhosphotyrosineBy similarity
Modified residuei512 – 5121PhosphothreonineBy similarity
Modified residuei531 – 5311Phosphotyrosine; by CSKBy similarity

Post-translational modificationi

Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling By similarity.By similarity
Palmitoylation at Cys-3 and Cys-6 regulate subcellular location.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ62844.
PRIDEiQ62844.

PTM databases

PhosphoSiteiQ62844.

Expressioni

Tissue specificityi

Detected in spinal cord oligodendrocytes (at protein level).1 Publication

Developmental stagei

Up-regulated during oligodendrocyte differentiation.1 Publication

Gene expression databases

GenevestigatoriQ62844.

Interactioni

Subunit structurei

Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity. Interacts with TOM1L1 (phosphorylated form). Interacts with SH2D1A and SLAMF1. Interacts with and phosphorylates ITCH, down-regulating its activity. Interacts with FASLG. Interacts with RUNX3. Interacts with KIT. Interacts with KDR (tyrosine phosphorylated). Interacts with EPHA8; possible downstream effector of EPHA8 in regulation of cell adhesion. Interacts with PTK2/FAK1; this interaction leads to PTK2/FAK1 phosphorylation and activation. Interacts with CAV1; this interaction couples integrins to the Ras-ERK pathway. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated) By similarity. Interacts (via SH3 domain) with KLHL2 (via N-terminus).By similarity1 Publication

Protein-protein interaction databases

BioGridi247215. 2 interactions.
IntActiQ62844. 1 interaction.
STRINGi10116.ENSRNOP00000000733.

Structurei

3D structure databases

ProteinModelPortaliQ62844.
SMRiQ62844. Positions 81-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 14362SH3PROSITE-ProRule annotationAdd
BLAST
Domaini149 – 24698SH2PROSITE-ProRule annotationAdd
BLAST
Domaini271 – 524254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ62844.
KOiK05703.
OMAiSHNSGYR.
OrthoDBiEOG7GTT2V.
PhylomeDBiQ62844.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62844-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCVQCKDKE AAKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY
60 70 80 90 100
NNFHAAGGQG LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD
110 120 130 140 150
LSFHKGEKFQ ILNSSEGDWW EARSLTTGET GYIPSNYVAP VDSIQAEEWY
160 170 180 190 200
FGKLGRKDAE RQLLSFGNPR GTFLIRESET TKGAYSLSIR DWDDMKGDHV
210 220 230 240 250
KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC RLVVPCHKGM
260 270 280 290 300
PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT
310 320 330 340 350
LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL
360 370 380 390 400
LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN
410 420 430 440 450
GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW
460 470 480 490 500
SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI
510 520 530
HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL
Length:537
Mass (Da):60,702
Last modified:November 1, 1996 - v1
Checksum:i11AE4420919DBF1C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35365 mRNA. Translation: AAA82942.1.
PIRiPT0199.
RefSeqiNP_036887.1. NM_012755.1.
UniGeneiRn.19361.
Rn.228993.

Genome annotation databases

EnsembliENSRNOT00000000733; ENSRNOP00000000733; ENSRNOG00000000596.
GeneIDi25150.
KEGGirno:25150.
UCSCiRGD:2641. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35365 mRNA. Translation: AAA82942.1 .
PIRi PT0199.
RefSeqi NP_036887.1. NM_012755.1.
UniGenei Rn.19361.
Rn.228993.

3D structure databases

ProteinModelPortali Q62844.
SMRi Q62844. Positions 81-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247215. 2 interactions.
IntActi Q62844. 1 interaction.
STRINGi 10116.ENSRNOP00000000733.

PTM databases

PhosphoSitei Q62844.

Proteomic databases

PaxDbi Q62844.
PRIDEi Q62844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000000733 ; ENSRNOP00000000733 ; ENSRNOG00000000596 .
GeneIDi 25150.
KEGGi rno:25150.
UCSCi RGD:2641. rat.

Organism-specific databases

CTDi 2534.
RGDi 2641. Fyn.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118938.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi Q62844.
KOi K05703.
OMAi SHNSGYR.
OrthoDBi EOG7GTT2V.
PhylomeDBi Q62844.
TreeFami TF351634.

Enzyme and pathway databases

Reactomei REACT_195025. Signaling by SCF-KIT.
REACT_196225. Regulation of signaling by CBL.
REACT_197451. Regulation of KIT signaling.
REACT_197531. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_198646. FCGR activation.
REACT_198729. Constitutive PI3K/AKT Signaling in Cancer.
REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_212418. DAP12 signaling.

Miscellaneous databases

NextBioi 605589.
PROi Q62844.

Gene expression databases

Genevestigatori Q62844.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Expression of three type mRNAs encoding rat FYN by alternative splicing within 5' untranslated region."
    Nemoto K., Sekimoto M., Kageyama H., Fukamachi K., Nemoto F., Ueyama T., Senba E., Tomita I.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SHRSPImported.
    Tissue: BrainImported.
  2. "Novel putative protein kinase clones from a rat large granular lymphocyte tumor cell line."
    Yue C.C.
    Mol. Immunol. 28:399-408(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Morphological differentiation of oligodendrocytes requires activation of Fyn tyrosine kinase."
    Osterhout D.J., Wolven A., Wolf R.M., Resh M.D., Chao M.V.
    J. Cell Biol. 145:1209-1218(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-299.
  4. "Process elongation of oligodendrocytes is promoted by the Kelch-related actin-binding protein Mayven."
    Jiang S., Avraham H.K., Park S.Y., Kim T.A., Bu X., Seng S., Avraham S.
    J. Neurochem. 92:1191-1203(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KLHL2, TISSUE SPECIFICITY.

Entry informationi

Entry nameiFYN_RAT
AccessioniPrimary (citable) accession number: Q62844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3