Reviewed,
UniProtKB/Swiss-Prot Q62844 (FYN_RAT)
Last modified
February 9, 2010.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Proto-oncogene tyrosine-protein kinase Fyn EC=2.7.10.2 Alternative name(s): Proto-oncogene c-Fyn p59-Fyn | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 537 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Ref.3 UniProtKB P06241 UniProtKB P39688 |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.3 |
| Cofactor | Manganese. Ref.3 |
| Enzyme regulation | Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site By similarity. |
| Subunit structure | Associates through its SH3 domain, to the p85 subunit of phosphatidylinositol 3-kinase. Interacts with the FYN-binding protein (FYB). Interacts with phosphorylated TOM1L1. Interacts with PAG1. Interacts with CD79A upon activation of the B-cell antigen receptor which increases FYN activity By similarity. Interacts with SH2D1A and SLAMF1. Interacts (via SH3 domain) with PRMT8 By similarity. |
| Subcellular location | Cell membrane. Note: Present and active in lipid rafts By similarity. Present in cell body and along the process of mature and developing oligodendroyctes. Ref.3 UniProtKB P39688 |
| Developmental stage | Up-regulated during oligodendrocyte differentiation. Ref.3 |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | SH2 domain SH3 domain |
| Ligand | ATP-binding Manganese Metal-binding Nucleotide-binding |
| Molecular function | Developmental protein Kinase Transferase Tyrosine-protein kinase |
| PTM | Lipoprotein Myristate Palmitate Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | ionotropic glutamate receptor signaling pathway Traceable author statement. Source: RGD peptidyl-tyrosine phosphorylationInferred from mutant phenotype. Source: RGD |
| Cellular component | mitochondrial envelope Inferred from expression pattern. Source: RGD plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity UniProtKB P06241 | ||||||
| Chain | 2 – 537 | 536 | Proto-oncogene tyrosine-protein kinase Fyn UniProtKB P06241 | PRO_0000282948 | |||||
Regions | |||||||||
| Domain | 82 – 143 | 62 | SH3 | ||||||
| Domain | 149 – 246 | 98 | SH2 | ||||||
| Domain | 271 – 524 | 254 | Protein kinase | ||||||
| Nucleotide binding | 277 – 285 | 9 | ATP By similarity UniProtKB P28523 | ||||||
Sites | |||||||||
| Active site | 390 | 1 | Proton acceptor By similarity UniProtKB P28523 | ||||||
| Binding site | 299 | 1 | ATP Ref.3 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 15 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 21 | 1 | Phosphoserine By similarity UniProtKB P06241 | ||||||
| Modified residue | 25 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 185 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 213 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 214 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 254 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 257 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 420 | 1 | Phosphotyrosine; by autocatalysis By similarity UniProtKB P06241 | ||||||
| Modified residue | 440 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 512 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 531 | 1 | Phosphotyrosine By similarity UniProtKB P39688 | ||||||
| Lipidation | 2 | 1 | N-myristoyl glycine By similarity UniProtKB P39688 | ||||||
| Lipidation | 3 | 1 | S-palmitoyl cysteine By similarity UniProtKB P39688 | ||||||
| Lipidation | 6 | 1 | S-palmitoyl cysteine By similarity UniProtKB P39688 | ||||||
Experimental info | |||||||||
| Mutagenesis | 299 | 1 | K → M: Loss of kinase activity and reduction in oligodendrocyte process extension and myelin membrane formation. Ref.3 | ||||||
Sequences
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References
| [1] | "Expression of three type mRNAs encoding rat FYN by alternative splicing within 5' untranslated region." Nemoto K., Sekimoto M., Kageyama H., Fukamachi K., Nemoto F., Ueyama T., Senba E., Tomita I. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SHRSP. Tissue: Brain. |
| [2] | "Novel putative protein kinase clones from a rat large granular lymphocyte tumor cell line." Yue C.C. Mol. Immunol. 28:399-408(1991) [PubMed: 2062320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Morphological differentiation of oligodendrocytes requires activation of Fyn tyrosine kinase." Osterhout D.J., Wolven A., Wolf R.M., Resh M.D., Chao M.V. J. Cell Biol. 145:1209-1218(1999) [PubMed: 10366594] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-299. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U35365 mRNA. Translation: AAA82942.1. |
| IPI | IPI00209210. |
| PIR | PT0199. |
| RefSeq | NP_036887.1. |
| UniGene | Rn.19361 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FYN based on UniProtKB P06241. |
| SMR | Q62844. Positions 81-142, 84-537. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q62844. |
PTM databases | |
| PhosphoSite | Q62844. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000000733; ENSRNOP00000000733; ENSRNOG00000000596; Rattus norvegicus. [Genome view] |
| GeneID | 25150. |
| KEGG | rno:25150. |
Organism-specific databases | |
| CTD | 25150. |
| RGD | 2641. Fyn. |
Phylogenomic databases | |
| eggNOG | maNOG10466. |
| HOVERGEN | Q62844. |
| InParanoid | Q62844. |
| OMA | TTIPNYN. |
| OrthoDB | EOG9PP12J. |
| PhylomeDB | Q62844. |
Enzyme and pathway databases | |
| BRENDA | 2.7.10.2. 248. |
Gene expression databases | |
| ArrayExpress | Q62844. |
| Genevestigator | Q62844. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020745. Tyr_kinase_non-rcpt_Fyn. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. [Graphical view] |
| Gene3D | G3DSA:3.30.505.10. SH2. 1 hit. |
| PANTHER | PTHR23256:SF256. Tyr_kinase_non-rcpt_Fyn. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit. |
| Pfam | PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view] |
| PRINTS | PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. |
| SMART | SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 605589. |
Entry information
| Entry name | FYN_RAT | ||||||||
| Accession | Primary (citable) accession number: Q62844 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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