Reviewed, UniProtKB/Swiss-Prot Q62840 (AMBN_RAT)

Names and origin

Protein names

Recommended name:
    Ameloblastin
      Short name=Amelin

Gene names

Name:

Ambn

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the mineralization and structural organization of enamel.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Ameloblast-specific.
Sequence similarities	Belongs to the ameloblastin family.

Ontologies

Keywords
Biological process	Biomineralization
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Gene Ontology (GO)
Biological process	biomineral formation Inferred from electronic annotation. Source: UniProtKB-KW odontogenesis of dentine-containing tooth Inferred from electronic annotation. Source: InterPro
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	structural constituent of tooth enamel Ref.2 Traceable author statement. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q62840-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62840-2)

The sequence of this isoform differs from the canonical sequence as follows:
     104-118: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 422	396	Ameloblastin		PRO_0000001195
Natural variations
	Alternative sequence	104 – 118	15	Missing in isoform 2.		VSP_000226
Experimental info
	Sequence conflict	324	1	M → I in CAA90414. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0F983772E9082A89	FASTA	422	45,206
	10 20 30 40 50 60 MSASKIPLFK MKGLLLFLSL VKMSLAVPAF PQQPGAQGMA PPGMASLSLE TMRQLGSLQG 70 80 90 100 110 120 LNALSQYSRL GFGKALNSLW LHGLLPPHNS FPWIGPREHE TQQYEYSLPV HPPPLPSQPS 130 140 150 160 170 180 LQPHQPGLKP FLQPTAATGV QVTPQKPGPH PPMHPGQLPL QEGELIAPDE PQVAPSENPP 190 200 210 220 230 240 TPEVPIMDFA DPQFPTVFQI AHSLSRGPMA HNKVPTFYPG MFYMSYGANQ LNAPARIGFM 250 260 270 280 290 300 SSEEMPGERG SPMAYGTLFP GYGGFRQTLR GLNQNSPKGG DFTVEVDSPV SVTKGPEKGE 310 320 330 340 350 360 GPEGSPLQEA SPDKGENPAL LSQMAPGAHA GLLAFPNDHI PNMARGPAGQ RLLGVTPAAA 370 380 390 400 410 420 DPLITPELAE VYETYGADVT TPLGDGEATM DITMSPDTQQ PPMPGNKVHQ PQVHNAWRFQ EP
	Isoform 2. Checksum: F7C5EDDA0A775642	FASTA	407	43,501
	10 20 30 40 50 60 MSASKIPLFK MKGLLLFLSL VKMSLAVPAF PQQPGAQGMA PPGMASLSLE TMRQLGSLQG 70 80 90 100 110 120 LNALSQYSRL GFGKALNSLW LHGLLPPHNS FPWIGPREHE TQQPSLQPHQ PGLKPFLQPT 130 140 150 160 170 180 AATGVQVTPQ KPGPHPPMHP GQLPLQEGEL IAPDEPQVAP SENPPTPEVP IMDFADPQFP 190 200 210 220 230 240 TVFQIAHSLS RGPMAHNKVP TFYPGMFYMS YGANQLNAPA RIGFMSSEEM PGERGSPMAY 250 260 270 280 290 300 GTLFPGYGGF RQTLRGLNQN SPKGGDFTVE VDSPVSVTKG PEKGEGPEGS PLQEASPDKG 310 320 330 340 350 360 ENPALLSQMA PGAHAGLLAF PNDHIPNMAR GPAGQRLLGV TPAAADPLIT PELAEVYETY 370 380 390 400 GADVTTPLGD GEATMDITMS PDTQQPPMPG NKVHQPQVHN AWRFQEP

References

[1]	"Full-length sequence, localization, and chromosomal mapping of ameloblastin. A novel tooth-specific gene." Krebsbach P.H., Lee S.K., Matsuki Y., Kozak C.A., Yamada K.M., Yamada Y. J. Biol. Chem. 271:4431-4435(1996) [PubMed: 8626794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Incisor.
[2]	"A novel gene expressed in rat ameloblasts codes for proteins with cell binding domains." Cerny R., Slaby I., Hammarstrom L., Wurtz T. J. Bone Miner. Res. 11:883-891(1996) [PubMed: 8797107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley. Tissue: Tooth.
[3]	"Gene structure of rat ameloblastin and specific expression in amelogenesis." Lee S.K., Kim S.M., Lee Y.J., Yamada K.M., Yamada Y., Chi J.G. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35097 mRNA. Translation: AAC52428.1. Z50083 mRNA. Translation: CAA90414.1. AY159302 , AY159295, AY159296, AY159297, AY159298, AY159299, AY159300 Genomic DNA. Translation: AAN71737.1.
IPI	IPI00209202. IPI00231718.
RefSeq	NP_037032.1.
UniGene	Rn.11370
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62840. 8 interactions.
STRING	Q62840.
Genome annotation databases
Ensembl	ENSRNOT00000005003; ENSRNOP00000005003; ENSRNOG00000003718; Rattus norvegicus. [Genome view]
GeneID	25376.
KEGG	rno:25376.
UCSC	NM_012900. rat. Z50083. rat.
Organism-specific databases
CTD	25376.
RGD	2101. Ambn.
Phylogenomic databases
eggNOG	roNOG05067.
HOVERGEN	Q62840.
InParanoid	Q62840.
OMA	HETQQYE.
OrthoDB	EOG9ZGRZK.
PhylomeDB	Q62840.
Gene expression databases
ArrayExpress	Q62840.
Genevestigator	Q62840.
GermOnline	ENSRNOG00000003718. Rattus norvegicus.
Family and domain databases
InterPro	IPR007798. Amelin. [Graphical view]
PANTHER	PTHR14115. Amelin. 1 hit.
Pfam	PF05111. Amelin. 1 hit. [Graphical view]
SMART	SM00817. Amelin. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606405.

Entry information

Entry name

AMBN_RAT

Accession

Primary (citable) accession number: Q62840
Secondary accession number(s): Q540J9, Q63043

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)