Reviewed,
UniProtKB/Swiss-Prot Q62829 (PAK3_RAT)
Last modified
October 13, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase PAK 3 EC=2.7.11.1 Alternative name(s): p21-activated kinase 3 Short name=PAK-3 Beta-PAK p65-PAK | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 544 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Key regulator of synapse formation and plasticity in the hippocampus. Ref.2 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium. |
| Enzyme regulation | Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-421 and allows the kinase domain to adopt an active structure By similarity. |
| Subunit structure | Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK By similarity. Interacts with the C-terminal of APP. |
| Tissue specificity | Detected at high levels in the brain and at low levels in the testis. Ref.2 |
| Developmental stage | Found in the embryonic CNS with little expression elsewhere. |
| Post-translational modification | Autophosphorylated when activated by CDC42/p21. Ref.3 |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Domain | SH3-binding |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Developmental protein Kinase Serine/threonine-protein kinase Transferase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Allosteric enzyme Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylationTraceable author statement. Source: RGD protein kinase cascadeTraceable author statement. Source: RGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW SH3 domain bindingInferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 544 | 544 | Serine/threonine-protein kinase PAK 3 | PRO_0000086473 | |||||
Regions | |||||||||
| Domain | 70 – 83 | 14 | CRIB | ||||||
| Domain | 268 – 519 | 252 | Protein kinase | ||||||
| Nucleotide binding | 274 – 282 | 9 | ATP By similarity | ||||||
| Region | 65 – 135 | 71 | Autoregulatory region By similarity | ||||||
| Region | 65 – 108 | 44 | GTPase-binding By similarity | ||||||
| Region | 84 – 267 | 184 | Linker | ||||||
Sites | |||||||||
| Active site | 387 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 297 | 1 | ATP Probable | ||||||
Amino acid modifications | |||||||||
| Modified residue | 50 | 1 | Phosphoserine; by autocatalysis Ref.3 | ||||||
| Modified residue | 139 | 1 | Phosphoserine; by autocatalysis Ref.3 | ||||||
| Modified residue | 421 | 1 | Phosphothreonine; by autocatalysis Ref.3 | ||||||
| Modified residue | 520 | 1 | N6-acetyllysine By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 297 | 1 | K → A: Loss of kinase activity. Ref.2 | ||||||
| Mutagenesis | 421 | 1 | T → E: Constitutively active. Ref.2 | ||||||
Sequences
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References
| [1] | "Molecular cloning of a new member of the p21-Cdc42/Rac-activated kinase (PAK) family." Manser E., Chong C., Zhao Z.-S., Leung T., Michael G., Hall C., Lim L. J. Biol. Chem. 270:25070-25078(1995) [PubMed: 7559638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE OF 37-56. Tissue: Brain. |
| [2] | "DNA synthesis and neuronal apoptosis caused by familial Alzheimer disease mutants of the amyloid precursor protein are mediated by the p21 activated kinase PAK3." McPhie D.L., Coopersmith R., Hines-Peralta A., Chen Y., Ivins K.J., Manly S.P., Kozlowski M.R., Neve K.A., Neve R.L. J. Neurosci. 23:6914-6927(2003) [PubMed: 12890786] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH APP, MUTAGENESIS OF LYS-297 AND THR-421. |
| [3] | "The mechanism of PAK activation. Autophosphorylation events in both regulatory and kinase domains control activity." Chong C., Tan L., Lim L., Manser E. J. Biol. Chem. 276:17347-17353(2001) [PubMed: 11278486] [Abstract] Cited for: PHOSPHORYLATION AT SER-50; SER-139 AND THR-421. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U33314 mRNA. Translation: AAC52268.1. | |
| IPI | IPI00327030. |
| PIR | A57597. |
| RefSeq | NP_062083.1. |
| UniGene | Rn.10128 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EES based on UniProtKB Q61036. |
| SMR | Q62829. Positions 73-141, 247-538. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q62829. |
PTM databases | |
| PhosphoSite | Q62829. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000006459; ENSRNOP00000006459; ENSRNOG00000004676; Rattus norvegicus. [Genome view] ENSRNOT00000033903; ENSRNOP00000029906; ENSRNOG00000004676; Rattus norvegicus. [Genome view] |
| GeneID | 29433. |
| KEGG | rno:29433. |
| UCSC | NM_019210. rat. |
Organism-specific databases | |
| CTD | 29433. |
| RGD | 3251. Pak3. |
Phylogenomic databases | |
| HOVERGEN | Q62829. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 248. |
Gene expression databases | |
| ArrayExpress | Q62829. |
| Genevestigator | Q62829. |
| GermOnline | ENSRNOG00000004676. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000095. PAK_box_Rho_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR015750. Ser/Thr_Kinase_Pak-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| PANTHER | PTHR22986:SF84. Pak_like. 1 hit. |
| Pfam | PF00786. PBD. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00285. PBD. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS50108. CRIB. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 609162. |
Entry information
| Entry name | PAK3_RAT | ||||||||
| Accession | Primary (citable) accession number: Q62829 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


