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Protein

Heterogeneous nuclear ribonucleoprotein M

Gene

Hnrnpm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA binding protein, binds avidly to poly(G) and poly(U) RNA homopolymers. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • protein antigen binding Source: RGD
  • protein domain specific binding Source: RGD
  • RNA binding Source: RGD

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: RGD
  • cellular response to carcinoembryonic antigen Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • positive regulation of protein import Source: RGD
  • positive regulation of protein localization to nucleus Source: RGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein M
Short name:
hnRNP M
Alternative name(s):
M4 protein
Gene namesi
Name:Hnrnpm
Synonyms:Hnrpm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620369. Hnrnpm.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000818662 – 690Heterogeneous nuclear ribonucleoprotein MAdd BLAST689

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei29PhosphoserineBy similarity1
Cross-linki37Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei85PhosphoserineBy similarity1
Cross-linki87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei133N6-acetyllysine; alternateBy similarity1
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei164PhosphoserineBy similarity1
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei237N6-acetyllysine; alternateBy similarity1
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki245Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki305Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei325PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1
Cross-linki341Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki348Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei357PhosphoserineBy similarity1
Modified residuei392PhosphoserineBy similarity1
Modified residuei412PhosphoserineBy similarity1
Modified residuei428PhosphoserineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei456Omega-N-methylarginineBy similarity1
Modified residuei488PhosphoserineBy similarity1
Modified residuei535PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1
Modified residuei578PhosphoserineBy similarity1
Modified residuei593PhosphoserineBy similarity1
Modified residuei597PhosphoserineBy similarity1
Cross-linki611Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei625PhosphothreonineBy similarity1
Cross-linki627Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei632N6-acetyllysineBy similarity1
Cross-linki645Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki652Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei658N6-acetyllysine; alternateBy similarity1
Cross-linki658Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki658Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei661PhosphoserineCombined sources1
Cross-linki676Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PeptideAtlasiQ62826.
PRIDEiQ62826.

PTM databases

iPTMnetiQ62826.
PhosphoSitePlusiQ62826.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including liver, heart, lung, skeletal muscle, kidney, stomach, large intestine, small intestine, pancreas, spleen, peritoneal macrophage and thyroid.1 Publication

Interactioni

Subunit structurei

Identified in the spliceosome C complex.By similarity

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • protein antigen binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi250540. 2 interactors.
IntActiQ62826. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ62826.
SMRiQ62826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 148RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini164 – 241RRM 2PROSITE-ProRule annotationAdd BLAST78
Repeati360 – 36516
Repeati367 – 37226
Repeati375 – 38036
Repeati386 – 39146
Repeati393 – 39856
Repeati400 – 40566
Repeati406 – 41176
Repeati413 – 41886
Repeati421 – 42696
Repeati428 – 433106
Repeati435 – 440116
Repeati442 – 447126
Repeati453 – 458136
Repeati460 – 465146
Repeati467 – 472156
Repeati474 – 479166
Repeati481 – 486176
Repeati488 – 493186
Repeati500 – 505196
Repeati507 – 512206
Repeati514 – 519216
Repeati522 – 527226
Repeati528 – 532235
Repeati535 – 540246
Repeati541 – 545255
Repeati548 – 553266
Repeati563 – 568276
Domaini613 – 689RRM 3PROSITE-ProRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 56827 X 6 AA repeats of [GEVSTPAN]-[ILMV]-[DE]-[RH]-[MLVI]-[GAV]Add BLAST209

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi260 – 607Gly-richAdd BLAST348

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiQ62826.
KOiK12887.
PhylomeDBiQ62826.

Family and domain databases

CDDicd12661. RRM3_hnRNPM. 1 hit.
InterProiView protein in InterPro
IPR024667. HnRNP_M.
IPR024666. HnRNP_M_PY-NLS.
IPR034990. hnRNPM_RRM3.
IPR035979. RBD_domain_sf.
IPR000504. RRM_dom.
PANTHERiPTHR23003:SF6. PTHR23003:SF6. 2 hits.
PfamiView protein in Pfam
PF11532. HnRNP_M. 1 hit.
PF00076. RRM_1. 3 hits.
SMARTiView protein in SMART
SM00360. RRM. 3 hits.
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiView protein in PROSITE
PS50102. RRM. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q62826-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGVEAAAE VAATEPKMEE ESGAPCVPSG NGAPVPKGEE RPTQNEKRKE
60 70 80 90 100
KNIKRGGNRF EPYANPTKRY RAFITNIPFD VKWQSLKDLV KEKVGEVTYV
110 120 130 140 150
ELLMDAEGKS RGCAVVEFKM EESMKKAAEV LNKHSLSGRP LKVKEDPDGE
160 170 180 190 200
HARRAMQKAG RLGSTVFVAN LDYKVGWKKL KEVFSMAGVV VRADILEDKD
210 220 230 240 250
GKSRGIGTVT FEQSIEAVQA ISMFNGQLLF DRPMHVKMDE RALPKGDFFP
260 270 280 290 300
PERPQQLPHG LGGIGMGLGP GGQPIDANHL NKGIGMGNLG PAGMGMEGIG
310 320 330 340 350
FGINKIGGME GPFGGGMENM GRFGSGMNMG RINEILSNAL KRGEIIAKQG
360 370 380 390 400
GSGAGGSVPG IERMGPGIDR ISGAGMERMG AGLGHGMDRV GSEIERMGLV
410 420 430 440 450
MDRMGSVERM GSGIERMGPL GLDHMASSIE RMGQTMERIG SGVERMGAGM
460 470 480 490 500
GFGLERMAAP IDRVGQTIER MGSGVERMGP AIERMGLSMD RMVPTGMGAG
510 520 530 540 550
LERMGPVMDR MATGLERMGA NNLERMGLER MGANSLERMG LERMGANSLE
560 570 580 590 600
RMGPAMGPAL GAGIERMGLA MGGAGGASFD RTIEMERGNF GGSFAGSFGG
610 620 630 640 650
AGGHAPGVAR KACQIFVRNL PFDFTWKMLK DKFNECGHVL YADIKMENGK
660 670 680 690
SKGCGVVKFE SPEVAERACR MMNGMKLSGR EIDVRIDRNA
Length:690
Mass (Da):73,782
Last modified:January 23, 2007 - v4
Checksum:i4FF9B6D6BD9B1F43
GO
Isoform 2 (identifier: Q62826-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-155: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:535
Mass (Da):56,674
Checksum:i96252F21F6507350
GO

Sequence cautioni

The sequence AAA83442 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55R → S AA sequence (PubMed:10947964).Curated1
Sequence conflicti352S → G in AAH88317 (PubMed:15489334).Curated1
Sequence conflicti582T → A in AAH88317 (PubMed:15489334).Curated1
Sequence conflicti615I → T AA sequence (PubMed:10947964).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0517661 – 155Missing in isoform 2. 1 PublicationAdd BLAST155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32577 mRNA. Translation: AAA83442.2. Different initiation.
BC088317 mRNA. Translation: AAH88317.1.
RefSeqiNP_001103381.1. NM_001109911.1.
NP_446328.2. NM_053876.2.
UniGeneiRn.10156.

Genome annotation databases

GeneIDi116655.
KEGGirno:116655.
UCSCiRGD:620369. rat. [Q62826-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHNRPM_RAT
AccessioniPrimary (citable) accession number: Q62826
Secondary accession number(s): Q5M815
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 133 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome