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Protein

Heterogeneous nuclear ribonucleoprotein M

Gene

Hnrnpm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA binding protein, binds avidly to poly(G) and poly(U) RNA homopolymers. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • nucleotide binding Source: InterPro
  • protein antigen binding Source: RGD
  • RNA binding Source: RGD

GO - Biological processi

  • cellular response to carcinoembryonic antigen Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • mRNA splicing, via spliceosome Source: InterPro
  • positive regulation of protein import Source: RGD
  • positive regulation of protein localization to nucleus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein M
Short name:
hnRNP M
Alternative name(s):
M4 protein
Gene namesi
Name:Hnrnpm
Synonyms:Hnrpm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620369. Hnrnpm.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: RGD
  • nuclear matrix Source: UniProtKB-SubCell
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 690689Heterogeneous nuclear ribonucleoprotein MPRO_0000081866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki17 – 17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei29 – 291PhosphoserineBy similarity
Cross-linki82 – 82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei133 – 1331N6-acetyllysineBy similarity
Cross-linki144 – 144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei237 – 2371N6-acetyllysineBy similarity
Modified residuei325 – 3251PhosphoserineBy similarity
Cross-linki348 – 348Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei357 – 3571PhosphoserineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity
Modified residuei428 – 4281PhosphoserineBy similarity
Modified residuei441 – 4411PhosphoserineBy similarity
Modified residuei488 – 4881PhosphoserineBy similarity
Modified residuei535 – 5351PhosphoserineCombined sources
Modified residuei548 – 5481PhosphoserineCombined sources
Modified residuei578 – 5781PhosphoserineBy similarity
Modified residuei593 – 5931PhosphoserineBy similarity
Modified residuei597 – 5971PhosphoserineBy similarity
Modified residuei632 – 6321N6-acetyllysineBy similarity
Cross-linki645 – 645Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei658 – 6581N6-acetyllysine; alternateBy similarity
Cross-linki658 – 658Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei661 – 6611PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ62826.

PTM databases

iPTMnetiQ62826.
PhosphoSiteiQ62826.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including liver, heart, lung, skeletal muscle, kidney, stomach, large intestine, small intestine, pancreas, spleen, peritoneal macrophage and thyroid.1 Publication

Interactioni

Subunit structurei

Identified in the spliceosome C complex.By similarity

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • protein antigen binding Source: RGD

Protein-protein interaction databases

BioGridi250540. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ62826.
SMRiQ62826. Positions 149-256, 612-690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 14879RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini164 – 24178RRM 2PROSITE-ProRule annotationAdd
BLAST
Repeati360 – 36561
Repeati367 – 37262
Repeati375 – 38063
Repeati386 – 39164
Repeati393 – 39865
Repeati400 – 40566
Repeati406 – 41167
Repeati413 – 41868
Repeati421 – 42669
Repeati428 – 433610
Repeati435 – 440611
Repeati442 – 447612
Repeati453 – 458613
Repeati460 – 465614
Repeati467 – 472615
Repeati474 – 479616
Repeati481 – 486617
Repeati488 – 493618
Repeati500 – 505619
Repeati507 – 512620
Repeati514 – 519621
Repeati522 – 527622
Repeati528 – 532523
Repeati535 – 540624
Repeati541 – 545525
Repeati548 – 553626
Repeati563 – 568627
Domaini613 – 68977RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 56820927 X 6 AA repeats of [GEVSTPAN]-[ILMV]-[DE]-[RH]-[MLVI]-[GAV]Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi260 – 607348Gly-richAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiQ62826.
KOiK12887.
PhylomeDBiQ62826.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR024667. HnRNP_M.
IPR024666. HnRNP_M_PY-NLS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23003:SF6. PTHR23003:SF6. 3 hits.
PfamiPF11532. HnRNP_M. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q62826-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGVEAAAE VAATEPKMEE ESGAPCVPSG NGAPVPKGEE RPTQNEKRKE
60 70 80 90 100
KNIKRGGNRF EPYANPTKRY RAFITNIPFD VKWQSLKDLV KEKVGEVTYV
110 120 130 140 150
ELLMDAEGKS RGCAVVEFKM EESMKKAAEV LNKHSLSGRP LKVKEDPDGE
160 170 180 190 200
HARRAMQKAG RLGSTVFVAN LDYKVGWKKL KEVFSMAGVV VRADILEDKD
210 220 230 240 250
GKSRGIGTVT FEQSIEAVQA ISMFNGQLLF DRPMHVKMDE RALPKGDFFP
260 270 280 290 300
PERPQQLPHG LGGIGMGLGP GGQPIDANHL NKGIGMGNLG PAGMGMEGIG
310 320 330 340 350
FGINKIGGME GPFGGGMENM GRFGSGMNMG RINEILSNAL KRGEIIAKQG
360 370 380 390 400
GSGAGGSVPG IERMGPGIDR ISGAGMERMG AGLGHGMDRV GSEIERMGLV
410 420 430 440 450
MDRMGSVERM GSGIERMGPL GLDHMASSIE RMGQTMERIG SGVERMGAGM
460 470 480 490 500
GFGLERMAAP IDRVGQTIER MGSGVERMGP AIERMGLSMD RMVPTGMGAG
510 520 530 540 550
LERMGPVMDR MATGLERMGA NNLERMGLER MGANSLERMG LERMGANSLE
560 570 580 590 600
RMGPAMGPAL GAGIERMGLA MGGAGGASFD RTIEMERGNF GGSFAGSFGG
610 620 630 640 650
AGGHAPGVAR KACQIFVRNL PFDFTWKMLK DKFNECGHVL YADIKMENGK
660 670 680 690
SKGCGVVKFE SPEVAERACR MMNGMKLSGR EIDVRIDRNA
Length:690
Mass (Da):73,782
Last modified:January 23, 2007 - v4
Checksum:i4FF9B6D6BD9B1F43
GO
Isoform 2 (identifier: Q62826-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-155: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:535
Mass (Da):56,674
Checksum:i96252F21F6507350
GO

Sequence cautioni

The sequence AAA83442.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551R → S AA sequence (PubMed:10947964).Curated
Sequence conflicti352 – 3521S → G in AAH88317 (PubMed:15489334).Curated
Sequence conflicti582 – 5821T → A in AAH88317 (PubMed:15489334).Curated
Sequence conflicti615 – 6151I → T AA sequence (PubMed:10947964).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 155155Missing in isoform 2. 1 PublicationVSP_051766Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32577 mRNA. Translation: AAA83442.2. Different initiation.
BC088317 mRNA. Translation: AAH88317.1.
RefSeqiNP_001103381.1. NM_001109911.1.
NP_446328.2. NM_053876.2.
UniGeneiRn.10156.

Genome annotation databases

GeneIDi116655.
KEGGirno:116655.
UCSCiRGD:620369. rat. [Q62826-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32577 mRNA. Translation: AAA83442.2. Different initiation.
BC088317 mRNA. Translation: AAH88317.1.
RefSeqiNP_001103381.1. NM_001109911.1.
NP_446328.2. NM_053876.2.
UniGeneiRn.10156.

3D structure databases

ProteinModelPortaliQ62826.
SMRiQ62826. Positions 149-256, 612-690.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250540. 2 interactions.

PTM databases

iPTMnetiQ62826.
PhosphoSiteiQ62826.

Proteomic databases

PRIDEiQ62826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116655.
KEGGirno:116655.
UCSCiRGD:620369. rat. [Q62826-1]

Organism-specific databases

CTDi4670.
RGDi620369. Hnrnpm.

Phylogenomic databases

HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiQ62826.
KOiK12887.
PhylomeDBiQ62826.

Miscellaneous databases

PROiQ62826.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR024667. HnRNP_M.
IPR024666. HnRNP_M_PY-NLS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23003:SF6. PTHR23003:SF6. 3 hits.
PfamiPF11532. HnRNP_M. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells."
    Bajenova O.V., Zimmer R., Stolper E., Salisbury-Rowswell J., Nanji A., Thomas P.
    J. Biol. Chem. 276:31067-31073(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: LiverImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Liver.
  3. "The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family."
    Kafasla P., Patrinou-Georgoula M., Guialis A.
    Biochem. J. 350:495-503(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 55-68; 182-196; 245-260 AND 612-625.
    Tissue: Liver1 Publication.
  4. "Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space."
    Segawa M., Niino K., Mineki R., Kaga N., Murayama K., Sugimoto K., Watanabe Y., Furukawa K., Horigome T.
    FEBS J. 272:4327-4338(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 60-68 AND 418-423, SUBCELLULAR LOCATION.
    Tissue: Liver.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535; SER-548 AND SER-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHNRPM_RAT
AccessioniPrimary (citable) accession number: Q62826
Secondary accession number(s): Q5M815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.