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Protein

Zinc finger protein 148

Gene

Znf148

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 148
Alternative name(s):
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89
Gene namesi
Name:Znf148
Synonyms:Zbp89, Zfp148
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi62063. Zfp148.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Zinc finger protein 148PRO_0000047429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei194 – 1941PhosphothreonineBy similarity
Modified residuei250 – 2501PhosphoserineBy similarity
Modified residuei301 – 3011PhosphoserineBy similarity
Modified residuei306 – 3061PhosphoserineCombined sources
Cross-linki356 – 356Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei412 – 4121PhosphoserineCombined sources
Modified residuei607 – 6071N6-acetyllysineBy similarity
Modified residuei665 – 6651PhosphoserineBy similarity
Modified residuei784 – 7841PhosphoserineCombined sources

Post-translational modificationi

Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62806.
PRIDEiQ62806.

PTM databases

iPTMnetiQ62806.

Expressioni

Tissue specificityi

Expressed in heart, lung, kidney, skeletal muscle, liver, brain and spleen.1 Publication

Gene expression databases

GenevisibleiQ62806. RN.

Interactioni

Subunit structurei

Interacts with HNRNPDL. Interacts with the 5FMC complex; the interaction requires association with CHTOP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002435.

Structurei

3D structure databases

ProteinModelPortaliQ62806.
SMRiQ62806. Positions 224-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063238.
HOGENOMiHOG000293204.
HOVERGENiHBG054247.
InParanoidiQ62806.
OMAiMCHENRD.
OrthoDBiEOG76QFGM.
PhylomeDBiQ62806.
TreeFamiTF331779.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIDDKLEGL FLKCGGIDEM QSSRAMVVMG GVSGQSAVSG ELQESVLQDR
60 70 80 90 100
SLPHQEILAA DEVLQESEMR QQDMISHDEL MVHEETVKND EEQTDTHERL
110 120 130 140 150
PQGLQYALNV PISVKQEITF TDVSEQLMRD KKQVREPVDL QKKKKRKQRS
160 170 180 190 200
PAKILTINED GSLGLKTPKS HVCEHCNAAF RTNYHLQRHV FIHTGEKPFQ
210 220 230 240 250
CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY HMERHKRTHS
260 270 280 290 300
GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED
310 320 330 340 350
SGFSTSPKDN SLPKKKRQKP EKKSSGMDKE SVLDKSDTKK DRNDYLPLYS
360 370 380 390 400
SSTKVKDEYM VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS
410 420 430 440 450
LKQPLEQSQT ISPLSTYEDS KVSKYAFELV DKQALLDSEG SADIDQVDNL
460 470 480 490 500
QEGPSKPVHS STNYDDAMQF LKKKRYLQAA SNNSREYALN VGTIASQPSV
510 520 530 540 550
TQAAVASVID ENTTASILDS QALNVEIKSN HDKNVIPDEV LQTLLDHYSH
560 570 580 590 600
KANGQHEISF SVADTEVTSS ISINSSDVPE VTQSENVGSS SQASSSDKAN
610 620 630 640 650
MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNP PAFSSIDKQV
660 670 680 690 700
YAAMPINSFR SGMNSPLRTT PDKSHFGLIV GDSQHPFPFS GDETNHASAT
710 720 730 740 750
STADFLDQVT SQKKAEAQPV HQAYQMSSFE QPFRAPYHGS RAGIATQFST
760 770 780 790
ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG MTSSPDATTG QTFG
Length:794
Mass (Da):88,748
Last modified:November 1, 1996 - v1
Checksum:i7CAED0E3E79EA756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30381 mRNA. Translation: AAC52958.1.
RefSeqiNP_113803.1. NM_031615.2.
UniGeneiRn.64671.

Genome annotation databases

EnsembliENSRNOT00000002435; ENSRNOP00000002435; ENSRNOG00000001789.
ENSRNOT00000086955; ENSRNOP00000071067; ENSRNOG00000001789.
GeneIDi58820.
KEGGirno:58820.
UCSCiRGD:62063. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30381 mRNA. Translation: AAC52958.1.
RefSeqiNP_113803.1. NM_031615.2.
UniGeneiRn.64671.

3D structure databases

ProteinModelPortaliQ62806.
SMRiQ62806. Positions 224-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002435.

PTM databases

iPTMnetiQ62806.

Proteomic databases

PaxDbiQ62806.
PRIDEiQ62806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002435; ENSRNOP00000002435; ENSRNOG00000001789.
ENSRNOT00000086955; ENSRNOP00000071067; ENSRNOG00000001789.
GeneIDi58820.
KEGGirno:58820.
UCSCiRGD:62063. rat.

Organism-specific databases

CTDi22661.
RGDi62063. Zfp148.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063238.
HOGENOMiHOG000293204.
HOVERGENiHBG054247.
InParanoidiQ62806.
OMAiMCHENRD.
OrthoDBiEOG76QFGM.
PhylomeDBiQ62806.
TreeFamiTF331779.

Miscellaneous databases

PROiQ62806.

Gene expression databases

GenevisibleiQ62806. RN.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ZBP-89, a Kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter."
    Merchant J.L., Iyer G.R., Taylor B.R., Kitchen J.R., Mortensen E.R., Wang Z., Flintoft R.J., Michel J., Bassel-Duby R.
    Mol. Cell. Biol. 16:6644-6653(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Pituitary adenoma.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306; SER-412 AND SER-784, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZN148_RAT
AccessioniPrimary (citable) accession number: Q62806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.