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Protein

Hyaluronidase PH-20

Gene

Spam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: RGD
  3. fusion of sperm to egg plasma membrane Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_294690. Interaction With Cumulus Cells.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface antigen 2B1
Sperm surface protein PH-20
Gene namesi
Name:Spam1
Synonyms:Ph20, Spam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620547. Spam1.

Subcellular locationi

GO - Cellular componenti

  1. acrosomal vesicle Source: Ensembl
  2. anchored component of membrane Source: UniProtKB-KW
  3. external side of plasma membrane Source: Ensembl
  4. membrane raft Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 512Removed in mature formSequence AnalysisPRO_0000012098
Signal peptidei1 – 3535By similarityAdd
BLAST
Chaini36 – ?Hyaluronidase PH-20PRO_0000012097

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 351By similarity
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 237By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi437 ↔ 464By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiQ62803.

Expressioni

Gene expression databases

GenevestigatoriQ62803.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009537.

Structurei

3D structure databases

ProteinModelPortaliQ62803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77606.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ62803.
KOiK01197.
OMAiWDAMSLA.
OrthoDBiEOG74J97S.
PhylomeDBiQ62803.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV
60 70 80 90 100
WVWNVPTEAC VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP
110 120 130 140 150
HIDAQQTEHH GGIPQKGDLT THLVKAKEDV ERYIPTDKLG LAIIDWEEWR
160 170 180 190 200
PTWMRNWTPK DIYRNKSIEL VQAADPAINI TEATVRAKAQ FEGAAKEFME
210 220 230 240 250
GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV EKKRNDDLDW
260 270 280 290 300
LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI
310 320 330 340 350
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG
360 370 380 390 400
CPILRQYMKT TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL
410 420 430 440 450
DPSSFSINVT EAGKYEVLGK PEVKDLEYFS EHFKCSCFSK MTCEETSDMR
460 470 480 490 500
SIQDVNVCMG DNVCIKATLG PNSAFHLLPG KGLLLMTTLA HILHHLPHDI
510
FVFPWKMLVS TP
Length:512
Mass (Da):58,412
Last modified:October 31, 1996 - v1
Checksum:i5ED01DA50F9153B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89999 mRNA. Translation: CAA62016.1.
BC081748 mRNA. Translation: AAH81748.1.
RefSeqiNP_446419.1. NM_053967.2.
UniGeneiRn.87544.

Genome annotation databases

EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
GeneIDi117037.
KEGGirno:117037.
UCSCiRGD:620547. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89999 mRNA. Translation: CAA62016.1.
BC081748 mRNA. Translation: AAH81748.1.
RefSeqiNP_446419.1. NM_053967.2.
UniGeneiRn.87544.

3D structure databases

ProteinModelPortaliQ62803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009537.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Proteomic databases

PRIDEiQ62803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
GeneIDi117037.
KEGGirno:117037.
UCSCiRGD:620547. rat.

Organism-specific databases

CTDi6677.
RGDi620547. Spam1.

Phylogenomic databases

eggNOGiNOG77606.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ62803.
KOiK01197.
OMAiWDAMSLA.
OrthoDBiEOG74J97S.
PhylomeDBiQ62803.
TreeFamiTF321598.

Enzyme and pathway databases

ReactomeiREACT_294690. Interaction With Cumulus Cells.

Miscellaneous databases

NextBioi619835.
PROiQ62803.

Gene expression databases

GenevestigatoriQ62803.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding."
    Hou S.T., Ma A., Jones R., Hall L.
    Mol. Reprod. Dev. 45:193-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiHYALP_RAT
AccessioniPrimary (citable) accession number: Q62803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 4, 2004
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.