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Q62803

- HYALP_RAT

UniProt

Q62803 - HYALP_RAT

Protein

Hyaluronidase PH-20

Gene

Spam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid By similarity.By similarity

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei147 – 1471Proton donorBy similarity

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell adhesion Source: RGD
    3. fusion of sperm to egg plasma membrane Source: RGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell adhesion

    Enzyme and pathway databases

    ReactomeiREACT_198292. Interaction With Cumulus Cells.

    Protein family/group databases

    CAZyiGH56. Glycoside Hydrolase Family 56.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase PH-20 (EC:3.2.1.35)
    Short name:
    Hyal-PH20
    Alternative name(s):
    Hyaluronoglucosaminidase PH-20
    Sperm adhesion molecule 1
    Sperm surface antigen 2B1
    Sperm surface protein PH-20
    Gene namesi
    Name:Spam1
    Synonyms:Ph20, Spam
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi620547. Spam1.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. anchored component of membrane Source: UniProtKB-KW
    3. external side of plasma membrane Source: Ensembl
    4. membrane raft Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei? – 512Removed in mature formSequence AnalysisPRO_0000012098
    Signal peptidei1 – 3535By similarityAdd
    BLAST
    Chaini36 – ?Hyaluronidase PH-20PRO_0000012097

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi60 ↔ 351By similarity
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi223 ↔ 237By similarity
    Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi376 ↔ 387By similarity
    Disulfide bondi381 ↔ 435By similarity
    Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi437 ↔ 464By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PRIDEiQ62803.

    Expressioni

    Gene expression databases

    GenevestigatoriQ62803.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000009537.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62803.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG77606.
    GeneTreeiENSGT00550000074476.
    HOGENOMiHOG000015133.
    HOVERGENiHBG052053.
    InParanoidiQ62803.
    KOiK01197.
    OMAiWDAMSLA.
    OrthoDBiEOG74J97S.
    PhylomeDBiQ62803.
    TreeFamiTF321598.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001439. Hyaluronidase_PH20.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
    PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
    PRINTSiPR00846. GLHYDRLASE56.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62803-1 [UniParc]FASTAAdd to Basket

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    MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV    50
    WVWNVPTEAC VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP 100
    HIDAQQTEHH GGIPQKGDLT THLVKAKEDV ERYIPTDKLG LAIIDWEEWR 150
    PTWMRNWTPK DIYRNKSIEL VQAADPAINI TEATVRAKAQ FEGAAKEFME 200
    GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV EKKRNDDLDW 250
    LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI 300
    FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG 350
    CPILRQYMKT TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL 400
    DPSSFSINVT EAGKYEVLGK PEVKDLEYFS EHFKCSCFSK MTCEETSDMR 450
    SIQDVNVCMG DNVCIKATLG PNSAFHLLPG KGLLLMTTLA HILHHLPHDI 500
    FVFPWKMLVS TP 512
    Length:512
    Mass (Da):58,412
    Last modified:November 1, 1996 - v1
    Checksum:i5ED01DA50F9153B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89999 mRNA. Translation: CAA62016.1.
    BC081748 mRNA. Translation: AAH81748.1.
    RefSeqiNP_446419.1. NM_053967.2.
    UniGeneiRn.87544.

    Genome annotation databases

    EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
    GeneIDi117037.
    KEGGirno:117037.
    UCSCiRGD:620547. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89999 mRNA. Translation: CAA62016.1 .
    BC081748 mRNA. Translation: AAH81748.1 .
    RefSeqi NP_446419.1. NM_053967.2.
    UniGenei Rn.87544.

    3D structure databases

    ProteinModelPortali Q62803.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000009537.

    Protein family/group databases

    CAZyi GH56. Glycoside Hydrolase Family 56.

    Proteomic databases

    PRIDEi Q62803.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009537 ; ENSRNOP00000009537 ; ENSRNOG00000007231 .
    GeneIDi 117037.
    KEGGi rno:117037.
    UCSCi RGD:620547. rat.

    Organism-specific databases

    CTDi 6677.
    RGDi 620547. Spam1.

    Phylogenomic databases

    eggNOGi NOG77606.
    GeneTreei ENSGT00550000074476.
    HOGENOMi HOG000015133.
    HOVERGENi HBG052053.
    InParanoidi Q62803.
    KOi K01197.
    OMAi WDAMSLA.
    OrthoDBi EOG74J97S.
    PhylomeDBi Q62803.
    TreeFami TF321598.

    Enzyme and pathway databases

    Reactomei REACT_198292. Interaction With Cumulus Cells.

    Miscellaneous databases

    NextBioi 619835.
    PROi Q62803.

    Gene expression databases

    Genevestigatori Q62803.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001439. Hyaluronidase_PH20.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
    PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
    PRINTSi PR00846. GLHYDRLASE56.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding."
      Hou S.T., Ma A., Jones R., Hall L.
      Mol. Reprod. Dev. 45:193-203(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiHYALP_RAT
    AccessioniPrimary (citable) accession number: Q62803
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3