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Q62803

- HYALP_RAT

UniProt

Q62803 - HYALP_RAT

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Protein
Hyaluronidase PH-20
Gene
Spam1, Ph20, Spam
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid By similarity.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471Proton donor By similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: RGD
  3. fusion of sperm to egg plasma membrane Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_198292. Interaction With Cumulus Cells.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface antigen 2B1
Sperm surface protein PH-20
Gene namesi
Name:Spam1
Synonyms:Ph20, Spam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi620547. Spam1.

Subcellular locationi

GO - Cellular componenti

  1. acrosomal vesicle Source: Ensembl
  2. anchored component of membrane Source: UniProtKB-KW
  3. external side of plasma membrane Source: Ensembl
  4. membrane raft Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 512Removed in mature form Reviewed predictionPRO_0000012098
Signal peptidei1 – 3535 By similarity
Add
BLAST
Chaini36 – ?Hyaluronidase PH-20PRO_0000012097

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 351 By similarity
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi165 – 1651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi179 – 1791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi223 ↔ 237 By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi376 ↔ 387 By similarity
Disulfide bondi381 ↔ 435 By similarity
Glycosylationi408 – 4081N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi437 ↔ 464 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiQ62803.

Expressioni

Gene expression databases

GenevestigatoriQ62803.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009537.

Structurei

3D structure databases

ProteinModelPortaliQ62803.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77606.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ62803.
KOiK01197.
OMAiWDAMSLA.
OrthoDBiEOG74J97S.
PhylomeDBiQ62803.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62803-1 [UniParc]FASTAAdd to Basket

« Hide

MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV    50
WVWNVPTEAC VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP 100
HIDAQQTEHH GGIPQKGDLT THLVKAKEDV ERYIPTDKLG LAIIDWEEWR 150
PTWMRNWTPK DIYRNKSIEL VQAADPAINI TEATVRAKAQ FEGAAKEFME 200
GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV EKKRNDDLDW 250
LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI 300
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG 350
CPILRQYMKT TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL 400
DPSSFSINVT EAGKYEVLGK PEVKDLEYFS EHFKCSCFSK MTCEETSDMR 450
SIQDVNVCMG DNVCIKATLG PNSAFHLLPG KGLLLMTTLA HILHHLPHDI 500
FVFPWKMLVS TP 512
Length:512
Mass (Da):58,412
Last modified:November 1, 1996 - v1
Checksum:i5ED01DA50F9153B0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89999 mRNA. Translation: CAA62016.1.
BC081748 mRNA. Translation: AAH81748.1.
RefSeqiNP_446419.1. NM_053967.2.
UniGeneiRn.87544.

Genome annotation databases

EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
GeneIDi117037.
KEGGirno:117037.
UCSCiRGD:620547. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89999 mRNA. Translation: CAA62016.1 .
BC081748 mRNA. Translation: AAH81748.1 .
RefSeqi NP_446419.1. NM_053967.2.
UniGenei Rn.87544.

3D structure databases

ProteinModelPortali Q62803.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000009537.

Protein family/group databases

CAZyi GH56. Glycoside Hydrolase Family 56.

Proteomic databases

PRIDEi Q62803.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009537 ; ENSRNOP00000009537 ; ENSRNOG00000007231 .
GeneIDi 117037.
KEGGi rno:117037.
UCSCi RGD:620547. rat.

Organism-specific databases

CTDi 6677.
RGDi 620547. Spam1.

Phylogenomic databases

eggNOGi NOG77606.
GeneTreei ENSGT00550000074476.
HOGENOMi HOG000015133.
HOVERGENi HBG052053.
InParanoidi Q62803.
KOi K01197.
OMAi WDAMSLA.
OrthoDBi EOG74J97S.
PhylomeDBi Q62803.
TreeFami TF321598.

Enzyme and pathway databases

Reactomei REACT_198292. Interaction With Cumulus Cells.

Miscellaneous databases

NextBioi 619835.
PROi Q62803.

Gene expression databases

Genevestigatori Q62803.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding."
    Hou S.T., Ma A., Jones R., Hall L.
    Mol. Reprod. Dev. 45:193-203(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiHYALP_RAT
AccessioniPrimary (citable) accession number: Q62803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3