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Q62803 (HYALP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronidase PH-20

Short name=Hyal-PH20
EC=3.2.1.35
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface antigen 2B1
Sperm surface protein PH-20
Gene names
Name:Spam1
Synonyms:Ph20, Spam
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid By similarity.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 By similarity
Chain36 – ?Hyaluronidase PH-20PRO_0000012097
Propeptide? – 512Removed in mature form PotentialPRO_0000012098

Sites

Active site1471Proton donor By similarity

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation1651N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation3681N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Disulfide bond60 ↔ 351 By similarity
Disulfide bond223 ↔ 237 By similarity
Disulfide bond376 ↔ 387 By similarity
Disulfide bond381 ↔ 435 By similarity
Disulfide bond437 ↔ 464 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q62803 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5ED01DA50F9153B0

FASTA51258,412
        10         20         30         40         50         60 
MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV WVWNVPTEAC 

        70         80         90        100        110        120 
VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP HIDAQQTEHH GGIPQKGDLT 

       130        140        150        160        170        180 
THLVKAKEDV ERYIPTDKLG LAIIDWEEWR PTWMRNWTPK DIYRNKSIEL VQAADPAINI 

       190        200        210        220        230        240 
TEATVRAKAQ FEGAAKEFME GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV 

       250        260        270        280        290        300 
EKKRNDDLDW LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI 

       310        320        330        340        350        360 
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG CPILRQYMKT 

       370        380        390        400        410        420 
TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL DPSSFSINVT EAGKYEVLGK 

       430        440        450        460        470        480 
PEVKDLEYFS EHFKCSCFSK MTCEETSDMR SIQDVNVCMG DNVCIKATLG PNSAFHLLPG 

       490        500        510 
KGLLLMTTLA HILHHLPHDI FVFPWKMLVS TP 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding."
Hou S.T., Ma A., Jones R., Hall L.
Mol. Reprod. Dev. 45:193-203(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89999 mRNA. Translation: CAA62016.1.
BC081748 mRNA. Translation: AAH81748.1.
RefSeqNP_446419.1. NM_053967.2.
UniGeneRn.87544.

3D structure databases

ProteinModelPortalQ62803.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000009537.

Protein family/group databases

CAZyGH56. Glycoside Hydrolase Family 56.

Proteomic databases

PRIDEQ62803.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
GeneID117037.
KEGGrno:117037.
UCSCRGD:620547. rat.

Organism-specific databases

CTD6677.
RGD620547. Spam1.

Phylogenomic databases

eggNOGNOG77606.
GeneTreeENSGT00550000074476.
HOGENOMHOG000015133.
HOVERGENHBG052053.
InParanoidQ62803.
KOK01197.
OMAWDAMSLA.
OrthoDBEOG74J97S.
PhylomeDBQ62803.
TreeFamTF321598.

Gene expression databases

GenevestigatorQ62803.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSPR00846. GLHYDRLASE56.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio619835.
PROQ62803.

Entry information

Entry nameHYALP_RAT
AccessionPrimary (citable) accession number: Q62803
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries