Q62803 (HYALP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase PH-20
Short name=Hyal-PH20
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface antigen 2B1
Sperm surface protein PH-20

Gene names

Name:	Spam1
Synonyms:	Ph20, Spam

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesion Traceable author statement Ref.1. Source: RGD fusion of sperm to egg plasma membrane Traceable author statement Ref.1. Source: RGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	By similarity
	Chain	36 – ?		Hyaluronidase PH-20		PRO_0000012097
	Propeptide	? – 512		Removed in mature form Potential		PRO_0000012098
Sites
	Active site	147	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 351		By similarity
	Disulfide bond	223 ↔ 237		By similarity
	Disulfide bond	376 ↔ 387		By similarity
	Disulfide bond	381 ↔ 435		By similarity
	Disulfide bond	437 ↔ 464		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62803 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5ED01DA50F9153B0	FASTA	512	58,412
	10 20 30 40 50 60 MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV WVWNVPTEAC 70 80 90 100 110 120 VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP HIDAQQTEHH GGIPQKGDLT 130 140 150 160 170 180 THLVKAKEDV ERYIPTDKLG LAIIDWEEWR PTWMRNWTPK DIYRNKSIEL VQAADPAINI 190 200 210 220 230 240 TEATVRAKAQ FEGAAKEFME GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV 250 260 270 280 290 300 EKKRNDDLDW LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI 310 320 330 340 350 360 FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG CPILRQYMKT 370 380 390 400 410 420 TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL DPSSFSINVT EAGKYEVLGK 430 440 450 460 470 480 PEVKDLEYFS EHFKCSCFSK MTCEETSDMR SIQDVNVCMG DNVCIKATLG PNSAFHLLPG 490 500 510 KGLLLMTTLA HILHHLPHDI FVFPWKMLVS TP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding." Hou S.T., Ma A., Jones R., Hall L. Mol. Reprod. Dev. 45:193-203(1996) [PubMed: 8914077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89999 mRNA. Translation: CAA62016.1. BC081748 mRNA. Translation: AAH81748.1.
IPI	IPI00209087.
RefSeq	NP_446419.1. NM_053967.1.
UniGene	Rn.87544.
3D structure databases
ProteinModelPortal	Q62803.
ModBase	Search...
Protein-protein interaction databases
STRING	Q62803.
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231.
GeneID	117037.
KEGG	rno:117037.
UCSC	NM_053967. rat.
Organism-specific databases
CTD	6677.
RGD	620547. Spam1.
Phylogenomic databases
eggNOG	maNOG04106.
GeneTree	ENSGT00550000074476.
HOVERGEN	HBG052053.
InParanoid	Q62803.
OMA	WDAMSLA.
OrthoDB	EOG4QNMWH.
PhylomeDB	Q62803.
Gene expression databases
ArrayExpress	Q62803.
Genevestigator	Q62803.
GermOnline	ENSRNOG00000007231. Rattus norvegicus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001439. Glyco_hydro_56_PH20. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01197.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit. PTHR11769:SF4. Glyco_hydro_56_PH20. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56. PR00848. SPERMPH20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	619835.

Entry information

Entry name

HYALP_RAT

Accession

Primary (citable) accession number: Q62803

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program