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Protein

28 kDa heat- and acid-stable phosphoprotein

Gene

Pdap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
28 kDa heat- and acid-stable phosphoprotein
Alternative name(s):
PDGF-associated protein
Short name:
PAP
PDGFA-associated protein 1
Short name:
PAP1
Gene namesi
Name:Pdap1
Synonyms:Haspp28
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620442. Pdap1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18118128 kDa heat- and acid-stable phosphoproteinPRO_0000083899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphothreonineCombined sources
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources
Modified residuei70 – 701PhosphotyrosineBy similarity
Modified residuei132 – 1321N6-acetyllysineBy similarity
Modified residuei164 – 1641N6-acetyllysineBy similarity
Modified residuei176 – 1761PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by several kinases in vitro. In vivo, can be phosphorylated by CK2.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62785.
PRIDEiQ62785.

PTM databases

iPTMnetiQ62785.
PhosphoSiteiQ62785.

Expressioni

Tissue specificityi

Present in all tissues tested, including brain, lung, spleen, kidney, liver, heart, and muscle, in decreasing order of abundance.

Gene expression databases

GenevisibleiQ62785. RN.

Interactioni

Protein-protein interaction databases

BioGridi249111. 2 interactions.
STRINGi10116.ENSRNOP00000001310.

Structurei

3D structure databases

ProteinModelPortaliQ62785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDAP1 family.Curated

Phylogenomic databases

eggNOGiKOG3375. Eukaryota.
ENOG4111T3A. LUCA.
GeneTreeiENSGT00390000018509.
HOGENOMiHOG000241539.
HOVERGENiHBG000319.
InParanoidiQ62785.
OMAiCTGRKGG.
OrthoDBiEOG7WX0C5.
PhylomeDBiQ62785.
TreeFamiTF324338.

Family and domain databases

InterProiIPR019380. Casein_kinase_sb_PP28.
[Graphical view]
PfamiPF10252. PP28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKGGRKGGH KGRVRQYTSP EEIDAQLQAE KQKANEEDEQ EEGGDGASGD
60 70 80 90 100
PKKEKKSLDS DESEDEDDDY QQKRKGVEGL IDIENPNRVA QTTKKVTQLD
110 120 130 140 150
LDGPKELSRR EREEIEKQKA KERYMKMHLA GKTEQAKADL ARLAIIRKQR
160 170 180
EEAARKKEEE RKAKDDATLS GKRMQSLSLN K
Length:181
Mass (Da):20,605
Last modified:November 1, 1996 - v1
Checksum:iC9A74B7C41174F87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → S in AAC52531 (PubMed:8780057).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26541 mRNA. Translation: AAC52455.1.
U41744 mRNA. Translation: AAC52531.1.
BC088140 mRNA. Translation: AAH88140.1.
PIRiS62782.
RefSeqiNP_072117.1. NM_022595.1.
UniGeneiRn.6158.

Genome annotation databases

EnsembliENSRNOT00000001310; ENSRNOP00000001310; ENSRNOG00000000990.
GeneIDi64527.
KEGGirno:64527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26541 mRNA. Translation: AAC52455.1.
U41744 mRNA. Translation: AAC52531.1.
BC088140 mRNA. Translation: AAH88140.1.
PIRiS62782.
RefSeqiNP_072117.1. NM_022595.1.
UniGeneiRn.6158.

3D structure databases

ProteinModelPortaliQ62785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249111. 2 interactions.
STRINGi10116.ENSRNOP00000001310.

PTM databases

iPTMnetiQ62785.
PhosphoSiteiQ62785.

Proteomic databases

PaxDbiQ62785.
PRIDEiQ62785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001310; ENSRNOP00000001310; ENSRNOG00000000990.
GeneIDi64527.
KEGGirno:64527.

Organism-specific databases

CTDi11333.
RGDi620442. Pdap1.

Phylogenomic databases

eggNOGiKOG3375. Eukaryota.
ENOG4111T3A. LUCA.
GeneTreeiENSGT00390000018509.
HOGENOMiHOG000241539.
HOVERGENiHBG000319.
InParanoidiQ62785.
OMAiCTGRKGG.
OrthoDBiEOG7WX0C5.
PhylomeDBiQ62785.
TreeFamiTF324338.

Miscellaneous databases

PROiQ62785.

Gene expression databases

GenevisibleiQ62785. RN.

Family and domain databases

InterProiIPR019380. Casein_kinase_sb_PP28.
[Graphical view]
PfamiPF10252. PP28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel casein kinase II substrate, HASPP28, from rat brain."
    Shen L., Huang K.P., Chen H.C., Huang F.L.
    Arch. Biochem. Biophys. 327:131-141(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Characterization of a novel platelet-derived growth factor-associated protein."
    Fischer W.H., Schubert D.
    J. Neurochem. 66:2213-2216(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
    Moser K., White F.M.
    J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 AND SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18; SER-57; SER-60 AND SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHAP28_RAT
AccessioniPrimary (citable) accession number: Q62785
Secondary accession number(s): Q62890
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.