Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/nucleoside cotransporter 2

Gene

Slc28a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent and purine-selective. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Accepts purine, analogs of purine nucleosides and uridine, and exhibits high affinity for adenosine.

Enzyme regulationi

Inhibited by formycin B, partially inhibited by purine analog ara-A.

GO - Molecular functioni

  • nucleoside:sodium symporter activity Source: RGD
  • nucleoside binding Source: InterPro
  • nucleoside transmembrane transporter activity Source: RGD

GO - Biological processi

  • protein localization to cell surface Source: RGD
  • retina homeostasis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.41.2.1. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/nucleoside cotransporter 2
Alternative name(s):
Concentrative nucleoside transporter 2
Short name:
CNT 2
Short name:
rCNT2
Na(+)/nucleoside cotransporter 2
Sodium-coupled nucleoside transporter 2
Sodium/purine nucleoside cotransporter
Short name:
SPNT
Solute carrier family 28 member 2
Gene namesi
Name:Slc28a2
Synonyms:Cnt2, Spnt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61840. Slc28a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei81 – 10121HelicalSequence AnalysisAdd
BLAST
Transmembranei105 – 12420HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16719HelicalSequence AnalysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST
Transmembranei234 – 25421HelicalSequence AnalysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence AnalysisAdd
BLAST
Transmembranei296 – 31520HelicalSequence AnalysisAdd
BLAST
Transmembranei337 – 35620HelicalSequence AnalysisAdd
BLAST
Transmembranei363 – 38220HelicalSequence AnalysisAdd
BLAST
Transmembranei424 – 44421HelicalSequence AnalysisAdd
BLAST
Transmembranei455 – 47521HelicalSequence AnalysisAdd
BLAST
Transmembranei530 – 55021HelicalSequence AnalysisAdd
BLAST
Transmembranei568 – 58821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 659659Sodium/nucleoside cotransporter 2PRO_0000070452Add
BLAST

Proteomic databases

PRIDEiQ62773.

PTM databases

PhosphoSiteiQ62773.

Expressioni

Tissue specificityi

Expressed in liver (in bile canalicular membrane vesicles (CMV) but not in sinusoidal vesicles), jejunum, spleen and heart. Also expressed in brain and skeletal muscle. Not expressed in kidney, muscle and lung.

Gene expression databases

GenevestigatoriQ62773.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1972.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ62773.
KOiK11536.
PhylomeDBiQ62773.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030212. CNT1/CNT2.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF11. PTHR10590:SF11. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSEGRKSA SQDTSENGME NPGLELMEVG NLEQGKTLEE VTQGHSLKDG
60 70 80 90 100
LGHSSLWRRI LQPFTKARSF YQRHAGLFKK ILLGLLCLAY AAYLLAACIL
110 120 130 140 150
NFRRALALFV ITCLVIFILA CHFLKKFFAK KSIRCLKPLK NTRLRLWLKR
160 170 180 190 200
VFMGAAVVGL ILWLALDTAQ RPEQLISFAG ICMFILILFA CSKHHSAVSW
210 220 230 240 250
RTVFWGLGLQ FVFGILVIRT EPGFNAFQWL GDQIQIFLAY TVEGSSFVFG
260 270 280 290 300
DTLVQSVFAF QSLPIIIFFG CVMSILYYLG LVQWVIQKIA WFLQITMGTT
310 320 330 340 350
AAETLAVAGN IFVGMTEAPL LIRPYLADMT LSEIHAVMTG GFATIAGTVL
360 370 380 390 400
GAFISFGIDA SSLISASVMA APCALALSKL VYPEVEESKF KSKEGVKLPR
410 420 430 440 450
GEERNILEAA SNGATDAIAL VANVAANLIA FLAVLAFINS TLSWLGEMVD
460 470 480 490 500
IHGLTFQVIC SYVLRPMVFM MGVQWADCPL VAEIVGVKFF INEFVAYQQL
510 520 530 540 550
SQYKNKRLSG VEEWINGEKQ WISVKAEIIA TFSLCGFANL TSIGITLGGL
560 570 580 590 600
TSMVPQRKSD LCKLVVRALF TGACVSFISA CMAGILYVPR GAETDCVSFL
610 620 630 640 650
NTNFTNRTYE TYVCCRELFQ STLLNGTNMP SFSGPWQDKE SSLRNLAKCC

DLYTSTVCA
Length:659
Mass (Da):72,615
Last modified:November 1, 1996 - v1
Checksum:i0CE9B49E5D1FE453
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti419 – 4191A → G in AAD00159 (PubMed:8967974).Curated
Sequence conflicti522 – 5221I → V in AAD00159 (PubMed:8967974).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25055 mRNA. Translation: AAA80640.1.
U66723 mRNA. Translation: AAD00159.1.
PIRiA57532.
RefSeqiNP_113852.1. NM_031664.1.
UniGeneiRn.10140.

Genome annotation databases

GeneIDi60423.
KEGGirno:60423.
UCSCiRGD:61840. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25055 mRNA. Translation: AAA80640.1.
U66723 mRNA. Translation: AAD00159.1.
PIRiA57532.
RefSeqiNP_113852.1. NM_031664.1.
UniGeneiRn.10140.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL1287616.

Protein family/group databases

TCDBi2.A.41.2.1. the concentrative nucleoside transporter (cnt) family.

PTM databases

PhosphoSiteiQ62773.

Proteomic databases

PRIDEiQ62773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60423.
KEGGirno:60423.
UCSCiRGD:61840. rat.

Organism-specific databases

CTDi9153.
RGDi61840. Slc28a2.

Phylogenomic databases

eggNOGiCOG1972.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ62773.
KOiK11536.
PhylomeDBiQ62773.

Miscellaneous databases

NextBioi612142.
PROiQ62773.

Gene expression databases

GenevestigatoriQ62773.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030212. CNT1/CNT2.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF11. PTHR10590:SF11. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Primary structure and functional expression of a cDNA encoding the bile canalicular, purine-specific Na(+)-nucleoside cotransporter."
    Che M., Ortiz D.F., Arias I.M.
    J. Biol. Chem. 270:13596-13599(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "Transport of adenosine by recombinant purine- and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes."
    Yao S.Y., Ng A.M., Ritzel M.W., Gati W.P., Cass C.E., Young J.D.
    Mol. Pharmacol. 50:1529-1535(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  3. "A nucleoside transporter is functionally linked to ectonucleotidases in rat liver canalicular membrane."
    Che M., Nishida T., Gatmaitan Z., Arias I.M.
    J. Biol. Chem. 267:9684-9688(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Liver.

Entry informationi

Entry nameiS28A2_RAT
AccessioniPrimary (citable) accession number: Q62773
Secondary accession number(s): Q9QWI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.