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Q62773

- S28A2_RAT

UniProt

Q62773 - S28A2_RAT

Protein

Sodium/nucleoside cotransporter 2

Gene

Slc28a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Sodium-dependent and purine-selective. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Accepts purine, analogs of purine nucleosides and uridine, and exhibits high affinity for adenosine.

    Enzyme regulationi

    Inhibited by formycin B, partially inhibited by purine analog ara-A.

    GO - Molecular functioni

    1. nucleoside:sodium symporter activity Source: RGD
    2. nucleoside binding Source: InterPro
    3. nucleoside transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. nucleoside transmembrane transport Source: GOC
    2. protein localization to cell surface Source: RGD
    3. retina homeostasis Source: RGD

    Keywords - Biological processi

    Transport

    Protein family/group databases

    TCDBi2.A.41.2.1. the concentrative nucleoside transporter (cnt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/nucleoside cotransporter 2
    Alternative name(s):
    Concentrative nucleoside transporter 2
    Short name:
    CNT 2
    Short name:
    rCNT2
    Na(+)/nucleoside cotransporter 2
    Sodium-coupled nucleoside transporter 2
    Sodium/purine nucleoside cotransporter
    Short name:
    SPNT
    Solute carrier family 28 member 2
    Gene namesi
    Name:Slc28a2
    Synonyms:Cnt2, Spnt
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61840. Slc28a2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 659659Sodium/nucleoside cotransporter 2PRO_0000070452Add
    BLAST

    Proteomic databases

    PRIDEiQ62773.

    PTM databases

    PhosphoSiteiQ62773.

    Expressioni

    Tissue specificityi

    Expressed in liver (in bile canalicular membrane vesicles (CMV) but not in sinusoidal vesicles), jejunum, spleen and heart. Also expressed in brain and skeletal muscle. Not expressed in kidney, muscle and lung.

    Gene expression databases

    GenevestigatoriQ62773.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei81 – 10121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei105 – 12420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei173 – 19321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei201 – 22121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei234 – 25421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei261 – 28121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei296 – 31520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei337 – 35620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei363 – 38220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei424 – 44421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei455 – 47521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei530 – 55021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei568 – 58821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1972.
    HOGENOMiHOG000267658.
    HOVERGENiHBG054073.
    InParanoidiQ62773.
    KOiK11536.
    PhylomeDBiQ62773.

    Family and domain databases

    InterProiIPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view]
    PANTHERiPTHR10590. PTHR10590. 1 hit.
    PfamiPF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00804. nupC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q62773-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKSEGRKSA SQDTSENGME NPGLELMEVG NLEQGKTLEE VTQGHSLKDG    50
    LGHSSLWRRI LQPFTKARSF YQRHAGLFKK ILLGLLCLAY AAYLLAACIL 100
    NFRRALALFV ITCLVIFILA CHFLKKFFAK KSIRCLKPLK NTRLRLWLKR 150
    VFMGAAVVGL ILWLALDTAQ RPEQLISFAG ICMFILILFA CSKHHSAVSW 200
    RTVFWGLGLQ FVFGILVIRT EPGFNAFQWL GDQIQIFLAY TVEGSSFVFG 250
    DTLVQSVFAF QSLPIIIFFG CVMSILYYLG LVQWVIQKIA WFLQITMGTT 300
    AAETLAVAGN IFVGMTEAPL LIRPYLADMT LSEIHAVMTG GFATIAGTVL 350
    GAFISFGIDA SSLISASVMA APCALALSKL VYPEVEESKF KSKEGVKLPR 400
    GEERNILEAA SNGATDAIAL VANVAANLIA FLAVLAFINS TLSWLGEMVD 450
    IHGLTFQVIC SYVLRPMVFM MGVQWADCPL VAEIVGVKFF INEFVAYQQL 500
    SQYKNKRLSG VEEWINGEKQ WISVKAEIIA TFSLCGFANL TSIGITLGGL 550
    TSMVPQRKSD LCKLVVRALF TGACVSFISA CMAGILYVPR GAETDCVSFL 600
    NTNFTNRTYE TYVCCRELFQ STLLNGTNMP SFSGPWQDKE SSLRNLAKCC 650
    DLYTSTVCA 659
    Length:659
    Mass (Da):72,615
    Last modified:November 1, 1996 - v1
    Checksum:i0CE9B49E5D1FE453
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti419 – 4191A → G in AAD00159. (PubMed:8967974)Curated
    Sequence conflicti522 – 5221I → V in AAD00159. (PubMed:8967974)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25055 mRNA. Translation: AAA80640.1.
    U66723 mRNA. Translation: AAD00159.1.
    PIRiA57532.
    RefSeqiNP_113852.1. NM_031664.1.
    UniGeneiRn.10140.

    Genome annotation databases

    GeneIDi60423.
    KEGGirno:60423.
    UCSCiRGD:61840. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25055 mRNA. Translation: AAA80640.1 .
    U66723 mRNA. Translation: AAD00159.1 .
    PIRi A57532.
    RefSeqi NP_113852.1. NM_031664.1.
    UniGenei Rn.10140.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1287616.

    Protein family/group databases

    TCDBi 2.A.41.2.1. the concentrative nucleoside transporter (cnt) family.

    PTM databases

    PhosphoSitei Q62773.

    Proteomic databases

    PRIDEi Q62773.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 60423.
    KEGGi rno:60423.
    UCSCi RGD:61840. rat.

    Organism-specific databases

    CTDi 9153.
    RGDi 61840. Slc28a2.

    Phylogenomic databases

    eggNOGi COG1972.
    HOGENOMi HOG000267658.
    HOVERGENi HBG054073.
    InParanoidi Q62773.
    KOi K11536.
    PhylomeDBi Q62773.

    Miscellaneous databases

    NextBioi 612142.
    PROi Q62773.

    Gene expression databases

    Genevestigatori Q62773.

    Family and domain databases

    InterProi IPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view ]
    PANTHERi PTHR10590. PTHR10590. 1 hit.
    Pfami PF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00804. nupC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of a cDNA encoding the bile canalicular, purine-specific Na(+)-nucleoside cotransporter."
      Che M., Ortiz D.F., Arias I.M.
      J. Biol. Chem. 270:13596-13599(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    2. "Transport of adenosine by recombinant purine- and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes."
      Yao S.Y., Ng A.M., Ritzel M.W., Gati W.P., Cass C.E., Young J.D.
      Mol. Pharmacol. 50:1529-1535(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Jejunum.
    3. "A nucleoside transporter is functionally linked to ectonucleotidases in rat liver canalicular membrane."
      Che M., Nishida T., Gatmaitan Z., Arias I.M.
      J. Biol. Chem. 267:9684-9688(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Tissue: Liver.

    Entry informationi

    Entry nameiS28A2_RAT
    AccessioniPrimary (citable) accession number: Q62773
    Secondary accession number(s): Q9QWI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3