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Q62771 (STA5A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducer and activator of transcription 5A
Alternative name(s):
Mammary gland factor
Gene names
Name:Stat5a
Synonyms:Mgf
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length793 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. Ref.2

Subunit structure

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation By similarity.

Tissue specificity

Expressed in heart, lung, and weakly in muscle.

Developmental stage

Detected both in virgin rats and after mammary gland involution. The level of STAT5A increases constantly during pregnancy, but decreases at the onset of lactation and remains lows throughout lactation.

Post-translational modification

Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity. Ref.2

ISGylated By similarity.

Sequence similarities

Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.

Ontologies

Keywords
   Biological processLactation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainSH2 domain
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJAK-STAT cascade

Inferred from direct assay PubMed 11064147. Source: RGD

lactation

Inferred from expression pattern Ref.1. Source: RGD

mammary gland involution

Inferred from direct assay PubMed 16227201. Source: RGD

multicellular organismal reproductive process

Inferred from expression pattern PubMed 17433024. Source: RGD

prolactin signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription, DNA-templated

Traceable author statement Ref.1. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 16946407. Source: RGD

response to nutrient levels

Inferred from expression pattern PubMed 16946407. Source: RGD

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11064147. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: RGD

cytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

double-stranded DNA binding

Inferred from direct assay PubMed 11064147Ref.1. Source: RGD

protein binding

Inferred from physical interaction PubMed 10720695. Source: RGD

sequence-specific DNA binding

Inferred from direct assay PubMed 11064147. Source: RGD

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 11064147. Source: RGD

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Stat5A1 (identifier: Q62771-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Stat5A2 (identifier: Q62771-2)

The sequence of this isoform differs from the canonical sequence as follows:
     740-740: N → K
     741-793: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 793793Signal transducer and activator of transcription 5A
PRO_0000182426

Regions

Domain589 – 68698SH2

Amino acid modifications

Modified residue1281Phosphoserine By similarity
Modified residue6941Phosphotyrosine; by JAK2 Ref.2

Natural variations

Alternative sequence7401N → K in isoform Stat5A2.
VSP_006288
Alternative sequence741 – 79353Missing in isoform Stat5A2.
VSP_006289

Sequences

Sequence LengthMass (Da)Tools
Isoform Stat5A1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C0C34EE3F5879FB5

FASTA79390,833
        10         20         30         40         50         60 
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN PQDRGQATQL 

        70         80         90        100        110        120 
LEGLVQELQK KAEHQVGEDG FVLKIKLGHY ATQLQNTYDR CPMELVRCIR HILYNEQRLV 

       130        140        150        160        170        180 
REANNCSSPA GVLVDAMSQK HLQINQTFEE LRLITQDTES ELKKLQQTQE YFIIQYQESL 

       190        200        210        220        230        240 
RIQAQFGQLA QLNPQERMSR ETALQQKQVS LETWLQREAQ TLQQYRVELV EKHQKTLQLL 

       250        260        270        280        290        300 
RKQQTIILDD ELIQWKRGQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 

       310        320        330        340        350        360 
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL 

       370        380        390        400        410        420 
NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC CVMEYHQATG TLSAHFRNMS 

       430        440        450        460        470        480 
LKRIKRADRR GAESVTEEKF TVLFESQFSV GSNELVFQVK TLSLPVVVIV HGSQDHNATA 

       490        500        510        520        530        540 
TVLWDNAFAE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNR 

       550        560        570        580        590        600 
RSNHLEDYNS MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 

       610        620        630        640        650        660 
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRD FSIRSLADRL 

       670        680        690        700        710        720 
GDLNYLIYVF PDRPKDEVFA KYYTPVLAKA VDGYVKPQIK QVVPEFVNAS ADAGANATYM 

       730        740        750        760        770        780 
DQAPSPVVCP QAHYNMYPPN PDPVLDQDGE FDLDETMDVA RHVEELLRRP MDSLDPRLSP 

       790 
PAGLFTSARS SLS 

« Hide

Isoform Stat5A2 [UniParc].

Checksum: E4449A0D984F6DC5
Show »

FASTA74084,970

References

[1]"Regulation of mammary gland factor/Stat5a during mammary gland development."
Kazansky A.V., Raught B., Lindsey S.M., Wang Y.-F., Rosen J.M.
Mol. Endocrinol. 9:1598-1609(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STAT5A1 AND STAT5A2).
Tissue: Mammary gland.
[2]"Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage."
Krejci P., Salazar L., Kashiwada T.A., Chlebova K., Salasova A., Thompson L.M., Bryja V., Kozubik A., Wilcox W.R.
PLoS ONE 3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT TYR-694 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U24175 mRNA. Translation: AAA98843.1.
RefSeqNP_058760.1. NM_017064.1. [Q62771-1]
UniGeneRn.154399.
Rn.228580.

3D structure databases

ProteinModelPortalQ62771.
SMRQ62771. Positions 138-690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247025. 2 interactions.
STRING10116.ENSRNOP00000062128.

PTM databases

PhosphoSiteQ62771.

Proteomic databases

PaxDbQ62771.
PRIDEQ62771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24918.
KEGGrno:24918.
UCSCRGD:3773. rat. [Q62771-1]

Organism-specific databases

CTD6776.
RGD3773. Stat5a.

Phylogenomic databases

eggNOGNOG245085.
HOGENOMHOG000230988.
HOVERGENHBG107486.
InParanoidQ62771.
KOK11223.

Enzyme and pathway databases

ReactomeREACT_212996. Signal Transduction.
REACT_227097. Immune System.

Gene expression databases

GenevestigatorQ62771.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERPTHR11801. PTHR11801. 1 hit.
PfamPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604855.
PROQ62771.

Entry information

Entry nameSTA5A_RAT
AccessionPrimary (citable) accession number: Q62771
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families