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Q62771

- STA5A_RAT

UniProt

Q62771 - STA5A_RAT

Protein

Signal transducer and activator of transcription 5A

Gene

Stat5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.1 Publication

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. double-stranded DNA binding Source: RGD
    3. protein binding Source: RGD
    4. sequence-specific DNA binding Source: RGD
    5. sequence-specific DNA binding transcription factor activity Source: RGD
    6. signal transducer activity Source: InterPro

    GO - Biological processi

    1. JAK-STAT cascade Source: RGD
    2. lactation Source: RGD
    3. mammary gland involution Source: RGD
    4. multicellular organismal reproductive process Source: RGD
    5. prolactin signaling pathway Source: UniProtKB
    6. regulation of transcription, DNA-templated Source: RGD
    7. response to ethanol Source: RGD
    8. response to nutrient levels Source: RGD
    9. transcription from RNA polymerase II promoter Source: RGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Lactation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_199232. Prolactin receptor signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal transducer and activator of transcription 5A
    Alternative name(s):
    Mammary gland factor
    Gene namesi
    Name:Stat5a
    Synonyms:Mgf
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3773. Stat5a.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytosol Source: Reactome
    3. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 793793Signal transducer and activator of transcription 5APRO_0000182426Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281PhosphoserineBy similarity
    Modified residuei694 – 6941Phosphotyrosine; by JAK21 Publication

    Post-translational modificationi

    Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity.By similarity
    ISGylated.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ62771.
    PRIDEiQ62771.

    PTM databases

    PhosphoSiteiQ62771.

    Expressioni

    Tissue specificityi

    Expressed in heart, lung, and weakly in muscle.

    Developmental stagei

    Detected both in virgin rats and after mammary gland involution. The level of STAT5A increases constantly during pregnancy, but decreases at the onset of lactation and remains lows throughout lactation.

    Gene expression databases

    GenevestigatoriQ62771.

    Interactioni

    Subunit structurei

    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi247025. 2 interactions.
    STRINGi10116.ENSRNOP00000062128.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62771.
    SMRiQ62771. Positions 138-690.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini589 – 68698SH2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transcription factor STAT family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain

    Phylogenomic databases

    eggNOGiNOG245085.
    HOGENOMiHOG000230988.
    HOVERGENiHBG107486.
    InParanoidiQ62771.
    KOiK11223.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.532.10. 1 hit.
    1.20.1050.20. 1 hit.
    2.60.40.630. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR013800. STAT_TF_alpha.
    IPR015988. STAT_TF_coiled-coil.
    IPR013801. STAT_TF_DNA-bd.
    IPR012345. STAT_TF_DNA-bd_sub.
    IPR013799. STAT_TF_prot_interaction.
    [Graphical view]
    PANTHERiPTHR11801. PTHR11801. 1 hit.
    PfamiPF00017. SH2. 1 hit.
    PF01017. STAT_alpha. 1 hit.
    PF02864. STAT_bind. 1 hit.
    PF02865. STAT_int. 1 hit.
    [Graphical view]
    SMARTiSM00252. SH2. 1 hit.
    SM00964. STAT_int. 1 hit.
    [Graphical view]
    SUPFAMiSSF47655. SSF47655. 1 hit.
    SSF48092. SSF48092. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Stat5A1 (identifier: Q62771-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN    50
    PQDRGQATQL LEGLVQELQK KAEHQVGEDG FVLKIKLGHY ATQLQNTYDR 100
    CPMELVRCIR HILYNEQRLV REANNCSSPA GVLVDAMSQK HLQINQTFEE 150
    LRLITQDTES ELKKLQQTQE YFIIQYQESL RIQAQFGQLA QLNPQERMSR 200
    ETALQQKQVS LETWLQREAQ TLQQYRVELV EKHQKTLQLL RKQQTIILDD 250
    ELIQWKRGQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 300
    QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA 350
    TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC 400
    CVMEYHQATG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV 450
    GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD 500
    KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNR RSNHLEDYNS 550
    MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 600
    QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRD 650
    FSIRSLADRL GDLNYLIYVF PDRPKDEVFA KYYTPVLAKA VDGYVKPQIK 700
    QVVPEFVNAS ADAGANATYM DQAPSPVVCP QAHYNMYPPN PDPVLDQDGE 750
    FDLDETMDVA RHVEELLRRP MDSLDPRLSP PAGLFTSARS SLS 793
    Length:793
    Mass (Da):90,833
    Last modified:November 1, 1997 - v1
    Checksum:iC0C34EE3F5879FB5
    GO
    Isoform Stat5A2 (identifier: Q62771-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         740-740: N → K
         741-793: Missing.

    Show »
    Length:740
    Mass (Da):84,970
    Checksum:iE4449A0D984F6DC5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei740 – 7401N → K in isoform Stat5A2. 1 PublicationVSP_006288
    Alternative sequencei741 – 79353Missing in isoform Stat5A2. 1 PublicationVSP_006289Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24175 mRNA. Translation: AAA98843.1.
    RefSeqiNP_058760.1. NM_017064.1. [Q62771-1]
    UniGeneiRn.154399.
    Rn.228580.

    Genome annotation databases

    GeneIDi24918.
    KEGGirno:24918.
    UCSCiRGD:3773. rat. [Q62771-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24175 mRNA. Translation: AAA98843.1 .
    RefSeqi NP_058760.1. NM_017064.1. [Q62771-1 ]
    UniGenei Rn.154399.
    Rn.228580.

    3D structure databases

    ProteinModelPortali Q62771.
    SMRi Q62771. Positions 138-690.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247025. 2 interactions.
    STRINGi 10116.ENSRNOP00000062128.

    PTM databases

    PhosphoSitei Q62771.

    Proteomic databases

    PaxDbi Q62771.
    PRIDEi Q62771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24918.
    KEGGi rno:24918.
    UCSCi RGD:3773. rat. [Q62771-1 ]

    Organism-specific databases

    CTDi 6776.
    RGDi 3773. Stat5a.

    Phylogenomic databases

    eggNOGi NOG245085.
    HOGENOMi HOG000230988.
    HOVERGENi HBG107486.
    InParanoidi Q62771.
    KOi K11223.

    Enzyme and pathway databases

    Reactomei REACT_199232. Prolactin receptor signaling.

    Miscellaneous databases

    NextBioi 604855.
    PROi Q62771.

    Gene expression databases

    Genevestigatori Q62771.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.532.10. 1 hit.
    1.20.1050.20. 1 hit.
    2.60.40.630. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR013800. STAT_TF_alpha.
    IPR015988. STAT_TF_coiled-coil.
    IPR013801. STAT_TF_DNA-bd.
    IPR012345. STAT_TF_DNA-bd_sub.
    IPR013799. STAT_TF_prot_interaction.
    [Graphical view ]
    PANTHERi PTHR11801. PTHR11801. 1 hit.
    Pfami PF00017. SH2. 1 hit.
    PF01017. STAT_alpha. 1 hit.
    PF02864. STAT_bind. 1 hit.
    PF02865. STAT_int. 1 hit.
    [Graphical view ]
    SMARTi SM00252. SH2. 1 hit.
    SM00964. STAT_int. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47655. SSF47655. 1 hit.
    SSF48092. SSF48092. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of mammary gland factor/Stat5a during mammary gland development."
      Kazansky A.V., Raught B., Lindsey S.M., Wang Y.-F., Rosen J.M.
      Mol. Endocrinol. 9:1598-1609(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STAT5A1 AND STAT5A2).
      Tissue: Mammary gland.
    2. "Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage."
      Krejci P., Salazar L., Kashiwada T.A., Chlebova K., Salasova A., Thompson L.M., Bryja V., Kozubik A., Wilcox W.R.
      PLoS ONE 3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT TYR-694 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3.

    Entry informationi

    Entry nameiSTA5A_RAT
    AccessioniPrimary (citable) accession number: Q62771
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3