Q62771 (STA5A_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 105.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Signal transducer and activator of transcription 5A Alternative name(s): Mammary gland factor | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 793 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. Ref.2 |
| Subunit structure | Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity. |
| Subcellular location | Cytoplasm By similarity. Nucleus By similarity. Note: Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation By similarity. |
| Tissue specificity | Expressed in heart, lung, and weakly in muscle. |
| Developmental stage | Detected both in virgin rats and after mammary gland involution. The level of STAT5A increases constantly during pregnancy, but decreases at the onset of lactation and remains lows throughout lactation. |
| Post-translational modification | Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity. Ref.2 |
| Sequence similarities | Belongs to the transcription factor STAT family. Contains 1 SH2 domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Stat5A1 (identifier: Q62771-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Stat5A2 (identifier: Q62771-2) The sequence of this isoform differs from the canonical sequence as follows: 740-740: N → K 741-793: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 793 | 793 | Signal transducer and activator of transcription 5A | PRO_0000182426 | |||||
Regions | |||||||||
| Domain | 589 – 686 | 98 | SH2 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 694 | 1 | Phosphotyrosine; by JAK2 Ref.2 | ||||||
Natural variations | |||||||||
| Alternative sequence | 740 | 1 | N → K in isoform Stat5A2. | VSP_006288 | |||||
| Alternative sequence | 741 – 793 | 53 | Missing in isoform Stat5A2. | VSP_006289 | |||||
Sequences
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References
| [1] | "Regulation of mammary gland factor/Stat5a during mammary gland development." Kazansky A.V., Raught B., Lindsey S.M., Wang Y.-F., Rosen J.M. Mol. Endocrinol. 9:1598-1609(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STAT5A1 AND STAT5A2). Tissue: Mammary gland. |
| [2] | "Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage." Krejci P., Salazar L., Kashiwada T.A., Chlebova K., Salasova A., Thompson L.M., Bryja V., Kozubik A., Wilcox W.R. PLoS ONE 3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT TYR-694 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U24175 mRNA. Translation: AAA98843.1. |
| IPI | IPI00208241. IPI00231852. |
| RefSeq | NP_058760.1. NM_017064.1. |
| UniGene | Rn.154399. Rn.228580. |
3D structure databases | |
| ProteinModelPortal | Q62771. |
| SMR | Q62771. Positions 138-690. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 10116.ENSRNOP00000062128. |
PTM databases | |
| PhosphoSite | Q62771. |
Proteomic databases | |
| PaxDb | Q62771. |
| PRIDE | Q62771. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 24918. |
| KEGG | rno:24918. |
| UCSC | RGD:3773. rat. |
Organism-specific databases | |
| CTD | 6776. |
| RGD | 3773. Stat5a. |
Phylogenomic databases | |
| eggNOG | NOG245085. |
| HOGENOM | HOG000230988. |
| HOVERGEN | HBG107486. |
| InParanoid | Q62771. |
| KO | K11223. |
| OrthoDB | EOG457560. |
Enzyme and pathway databases | |
| Reactome | REACT_109781. Immune System. REACT_111984. Signal Transduction. |
Gene expression databases | |
| ArrayExpress | Q62771. |
| Genevestigator | Q62771. |
| GermOnline | ENSRNOG00000019496. Rattus norvegicus. |
Family and domain databases | |
| Gene3D | 1.10.238.10. 1 hit. 1.10.532.10. 1 hit. 1.20.1050.20. 1 hit. 2.60.40.630. 1 hit. 3.30.505.10. 1 hit. |
| InterPro | IPR011992. EF-hand-like_dom. IPR008967. p53-like_TF_DNA-bd. IPR000980. SH2. IPR013800. STAT_TF_alpha. IPR015988. STAT_TF_coiled-coil. IPR001217. STAT_TF_core. IPR013801. STAT_TF_DNA-bd. IPR012345. STAT_TF_DNA-bd_sub. IPR013799. STAT_TF_prot_interaction. [Graphical view] |
| PANTHER | PTHR11801. PTHR11801. 1 hit. |
| Pfam | PF00017. SH2. 1 hit. PF01017. STAT_alpha. 1 hit. PF02864. STAT_bind. 1 hit. PF02865. STAT_int. 1 hit. [Graphical view] |
| SMART | SM00252. SH2. 1 hit. SM00964. STAT_int. 1 hit. [Graphical view] |
| SUPFAM | SSF49417. P53_like_DNA_bnd. 1 hit. SSF47655. STAT. 1 hit. SSF48092. STAT. 1 hit. |
| PROSITE | PS50001. SH2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 604855. |
Entry information
| Entry name | STA5A_RAT | ||||||||
| Accession | Primary (citable) accession number: Q62771 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |