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Q62771

- STA5A_RAT

UniProt

Q62771 - STA5A_RAT

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Protein
Signal transducer and activator of transcription 5A
Gene
Stat5a, Mgf
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. double-stranded DNA binding Source: RGD
  3. protein binding Source: RGD
  4. sequence-specific DNA binding Source: RGD
  5. sequence-specific DNA binding transcription factor activity Source: RGD
  6. signal transducer activity Source: InterPro

GO - Biological processi

  1. JAK-STAT cascade Source: RGD
  2. lactation Source: RGD
  3. mammary gland involution Source: RGD
  4. multicellular organismal reproductive process Source: RGD
  5. prolactin signaling pathway Source: UniProtKB
  6. regulation of transcription, DNA-templated Source: RGD
  7. response to ethanol Source: RGD
  8. response to nutrient levels Source: RGD
  9. transcription from RNA polymerase II promoter Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Lactation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_199232. Prolactin receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 5A
Alternative name(s):
Mammary gland factor
Gene namesi
Name:Stat5a
Synonyms:Mgf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3773. Stat5a.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation By similarity.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytosol Source: Reactome
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Signal transducer and activator of transcription 5A
PRO_0000182426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity
Modified residuei694 – 6941Phosphotyrosine; by JAK21 Publication

Post-translational modificationi

Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity.1 Publication
ISGylated By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62771.
PRIDEiQ62771.

PTM databases

PhosphoSiteiQ62771.

Expressioni

Tissue specificityi

Expressed in heart, lung, and weakly in muscle.

Developmental stagei

Detected both in virgin rats and after mammary gland involution. The level of STAT5A increases constantly during pregnancy, but decreases at the onset of lactation and remains lows throughout lactation.

Gene expression databases

GenevestigatoriQ62771.

Interactioni

Subunit structurei

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity.

Protein-protein interaction databases

BioGridi247025. 2 interactions.
STRINGi10116.ENSRNOP00000062128.

Structurei

3D structure databases

ProteinModelPortaliQ62771.
SMRiQ62771. Positions 138-690.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini589 – 68698SH2
Add
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG245085.
HOGENOMiHOG000230988.
HOVERGENiHBG107486.
InParanoidiQ62771.
KOiK11223.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERiPTHR11801. PTHR11801. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMiSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Stat5A1 (identifier: Q62771-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN    50
PQDRGQATQL LEGLVQELQK KAEHQVGEDG FVLKIKLGHY ATQLQNTYDR 100
CPMELVRCIR HILYNEQRLV REANNCSSPA GVLVDAMSQK HLQINQTFEE 150
LRLITQDTES ELKKLQQTQE YFIIQYQESL RIQAQFGQLA QLNPQERMSR 200
ETALQQKQVS LETWLQREAQ TLQQYRVELV EKHQKTLQLL RKQQTIILDD 250
ELIQWKRGQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 300
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA 350
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC 400
CVMEYHQATG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV 450
GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD 500
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNR RSNHLEDYNS 550
MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 600
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRD 650
FSIRSLADRL GDLNYLIYVF PDRPKDEVFA KYYTPVLAKA VDGYVKPQIK 700
QVVPEFVNAS ADAGANATYM DQAPSPVVCP QAHYNMYPPN PDPVLDQDGE 750
FDLDETMDVA RHVEELLRRP MDSLDPRLSP PAGLFTSARS SLS 793
Length:793
Mass (Da):90,833
Last modified:November 1, 1997 - v1
Checksum:iC0C34EE3F5879FB5
GO
Isoform Stat5A2 (identifier: Q62771-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     740-740: N → K
     741-793: Missing.

Show »
Length:740
Mass (Da):84,970
Checksum:iE4449A0D984F6DC5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei740 – 7401N → K in isoform Stat5A2.
VSP_006288
Alternative sequencei741 – 79353Missing in isoform Stat5A2.
VSP_006289Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24175 mRNA. Translation: AAA98843.1.
RefSeqiNP_058760.1. NM_017064.1. [Q62771-1]
UniGeneiRn.154399.
Rn.228580.

Genome annotation databases

GeneIDi24918.
KEGGirno:24918.
UCSCiRGD:3773. rat. [Q62771-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24175 mRNA. Translation: AAA98843.1 .
RefSeqi NP_058760.1. NM_017064.1. [Q62771-1 ]
UniGenei Rn.154399.
Rn.228580.

3D structure databases

ProteinModelPortali Q62771.
SMRi Q62771. Positions 138-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247025. 2 interactions.
STRINGi 10116.ENSRNOP00000062128.

PTM databases

PhosphoSitei Q62771.

Proteomic databases

PaxDbi Q62771.
PRIDEi Q62771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24918.
KEGGi rno:24918.
UCSCi RGD:3773. rat. [Q62771-1 ]

Organism-specific databases

CTDi 6776.
RGDi 3773. Stat5a.

Phylogenomic databases

eggNOGi NOG245085.
HOGENOMi HOG000230988.
HOVERGENi HBG107486.
InParanoidi Q62771.
KOi K11223.

Enzyme and pathway databases

Reactomei REACT_199232. Prolactin receptor signaling.

Miscellaneous databases

NextBioi 604855.
PROi Q62771.

Gene expression databases

Genevestigatori Q62771.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view ]
PANTHERi PTHR11801. PTHR11801. 1 hit.
Pfami PF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view ]
SMARTi SM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view ]
SUPFAMi SSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regulation of mammary gland factor/Stat5a during mammary gland development."
    Kazansky A.V., Raught B., Lindsey S.M., Wang Y.-F., Rosen J.M.
    Mol. Endocrinol. 9:1598-1609(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STAT5A1 AND STAT5A2).
    Tissue: Mammary gland.
  2. "Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage."
    Krejci P., Salazar L., Kashiwada T.A., Chlebova K., Salasova A., Thompson L.M., Bryja V., Kozubik A., Wilcox W.R.
    PLoS ONE 3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT TYR-694 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3.

Entry informationi

Entry nameiSTA5A_RAT
AccessioniPrimary (citable) accession number: Q62771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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