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Protein

Protein unc-13 homolog B

Gene

Unc13b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP), Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri490 – 54051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • positive regulation of inhibitory postsynaptic potential Source: ParkinsonsUK-UCL
  • positive regulation of synaptic vesicle priming Source: ParkinsonsUK-UCL
  • spontaneous neurotransmitter secretion Source: ParkinsonsUK-UCL
  • synaptic transmission Source: ParkinsonsUK-UCL
  • synaptic vesicle priming Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-13 homolog B
Alternative name(s):
Munc13-2
Gene namesi
Name:Unc13b
Synonyms:Unc13h2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619723. Unc13b.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: ParkinsonsUK-UCL
  • presynapse Source: GOC
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16221622Protein unc-13 homolog BPRO_0000188577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei307 – 3071PhosphoserineBy similarity
Modified residuei378 – 3781PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62769.
PRIDEiQ62769.

Expressioni

Tissue specificityi

Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain, predominantly in cerebral cortex, hippocampus and striatum.3 Publications

Developmental stagei

First detected at birth, after which expression level is steadily increasing until it reaches a plateau at P15.1 Publication

Interactioni

Subunit structurei

Interacts with RIMS1.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058802.

Structurei

3D structure databases

ProteinModelPortaliQ62769.
SMRiQ62769. Positions 1-150, 491-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7979C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini600 – 706107C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini1025 – 1168144MHD1PROSITE-ProRule annotationAdd
BLAST
Domaini1275 – 1417143MHD2PROSITE-ProRule annotationAdd
BLAST
Domaini1456 – 1561106C2 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1210 – 123122Sequence analysisAdd
BLAST

Domaini

The C2 domains are not involved in calcium-dependent phospholipid binding.

Sequence similaritiesi

Belongs to the unc-13 family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation
Contains 1 MHD1 (MUNC13 homology domain 1) domain.PROSITE-ProRule annotation
Contains 1 MHD2 (MUNC13 homology domain 2) domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri490 – 54051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
HOGENOMiHOG000231404.
HOVERGENiHBG057340.
InParanoidiQ62769.
KOiK15293.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 2 hits.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62769-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM
60 70 80 90 100
FEISRLDLGL SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE
110 120 130 140 150
AETLMKNDEI CGTKNPTPHK ILLGTRFELP FDIPEEEARY WTYKLEQINA
160 170 180 190 200
LADDNEYSSQ EESQRKLLPT AAAQCRHWTY LGWGEHQTFE DPDSAVDDRD
210 220 230 240 250
SDYRSETSNS APPPYHTTTQ PNASAHQFPM PVPLPQQLFL QGSSRDSCND
260 270 280 290 300
SMQSYDLDYP ERRALSPTSS SRYGSSCNVS RGSSLLSELD QYHEQDDDGR
310 320 330 340 350
ERDSIHSSHS YGSLSRDGQA GLGEQEKALE VACESQKEKT GESKERDDAT
360 370 380 390 400
VCPPSDLMLH KDLTLGPQES FPEEKASSPF TQARAHWFRA VTKVRLQLQE
410 420 430 440 450
ISDDGDPSLP QWLPEGPAGG LYGIDSMPDL RRKKPLPLVS DLAMSLVQSR
460 470 480 490 500
KAGITSAMAT RTSLKDEDLK SHVYKKTLQA LIYPISCTTP HNFEVWSATT
510 520 530 540 550
PTYCYECEGL LWGLARQGMR CSECGVKCHE KCQDLLNADC LQRAAEKSSK
560 570 580 590 600
HGAEDRTQNI IMAMKDRMKI RERNKPEIFE VIRDVFTVSK VAHVQQMKTV
610 620 630 640 650
KQSVLDGTSK WSAKITITVV CAQGLQAKDK TGSSDPYVTV QVGKTKKRTK
660 670 680 690 700
TIFGNLNPVW EEKFHFECHN SSDRIKVRVW DEDDDIKSRV KQRLKRESDD
710 720 730 740 750
FLGQTIIEVR TLSGEMDVWY NLEKRTDKSA VSGAIRLQIN VEIKGEEKVA
760 770 780 790 800
PYHVQYTCLH ENLFHYLTDI QGSGGVWIPD ARGDDAWKVY FDETAQEIVD
810 820 830 840 850
EFAMRYGIES IYQAMTHFAC LSSKYMCPGV PAVMSTLLAN INAYYAHTTA
860 870 880 890 900
STNVSASDRF AASNFGKERF VKLLDQLHNS LRIDLSTYRN NFPAGSPERL
910 920 930 940 950
QDLKSTVDLL TSITFFRMKV QELQSPPRAS QVVKDCVKAC LNSTYEYIFN
960 970 980 990 1000
NCHDLYSHQY QLQEQPLEEP GPSIRNLDFW PKLITLIVSI IEEDKNSYTP
1010 1020 1030 1040 1050
VLNQFPQELN VGKVSAEVMW HLFAQDMKYA LEEHEKDRLC KSADYMNLHF
1060 1070 1080 1090 1100
KVKWLHNEYV RELPALQGQV PEYPAWFEQF VLQWLDENED VSLEFLRGGL
1110 1120 1130 1140 1150
ERDKRDGFQQ TSEHALFSCS VVDVFTQLNQ SFEIIRKLEC PDPSILAHYM
1160 1170 1180 1190 1200
RRFAKTIGKV LIQYADILSK NFPAYCTKER LPCILMNNMQ QLRVQLEKMF
1210 1220 1230 1240 1250
EAMGGKELDS EAADSLKELQ VKLNTVLDEL SMVFGNSFQV RIDECVRQMA
1260 1270 1280 1290 1300
DILGQVRGTG NASPNARASV AQDADSVLRP LMDFLDGNLT LFATVCEKTV
1310 1320 1330 1340 1350
LKRVLKELWR VVMNTMERVI VLPPLIDQTG TQLILTAAKE LSQLSKLKDH
1360 1370 1380 1390 1400
MVREETRNLT PKQCAVLDLA LDTVKQYFHA GGNGLKKTFL EKSPDLQSLR
1410 1420 1430 1440 1450
YALSLYTQTT DTLIKTFVRS QTAQVHDGKG IRFTANEDIR PEKGAGVDDP
1460 1470 1480 1490 1500
VGEVSIQVDL FTHPGTGEHK VTVKVVAAND LKWQTAGMFR PFVEVTMVGP
1510 1520 1530 1540 1550
HQSDKKRKFT TKSKSNSWTP KYNETFHFLL GNEEGPEAYE LQICVKDYCF
1560 1570 1580 1590 1600
AREDRVIGLA VMPLRDVAAK GSCACWCPLG RKIHMDETGM TILRILSQRS
1610 1620
NDEVAREFVK LKSESRSIEE GS
Length:1,622
Mass (Da):184,063
Last modified:August 16, 2004 - v2
Checksum:i885C210A13086ACD
GO
Isoform 2 (identifier: Q62769-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-400: MSLLCVRVKR...VTKVRLQLQE → MKRLLRESEE...PSEGKREALQ

Show »
Length:1,985
Mass (Da):222,076
Checksum:iAA3A008870DAC5E4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 400400MSLLC…LQLQE → MKRLLRESEEEIMLTLGPSS SLSPDQVRTETVCIVKGKST GPTGSLPEDNFPPPCCESAD STTSGERDRNLAQLGSFEQQ ASSQPSLACTACASGSDSRE LSPASITSCSEPSERNKARP IFPRGPGQRCRHEHQEPLGD VVEYIIRELQGISRLQSEIA ELQQHLNQVRGSVDEVSSCV DSVLSEIEGLHVGSSSLGKV RHGEKAQELHVERSREEAIL YLYGLPEHDGESTVELVDNF LAKHLCVNGMQCNRYVREAY RAGTAPAPRPTVVKLVHPEH RDLILQKSILLQSVGVRVAT REEPVWPEGCKNPPKESLSC LQQFQDHSRNHQGKPALQLE TGNRRQMSGPHQMRTQNQHR ELQASEHQGLSFLPKDGSAK QSDVSKLQDEVKGTSGAPQV ISDPCGELSLLHQLEGSSPV LIPKEEDCGKLQIFKQDSQE HKACNVTKLQSDCNNAIKAS SCLSLSGPLKAEKVNAEDRM LGGEDGLDILSPKQLEDLLA DKSRRFATLNPDSAVEEVII GPETFSNMVHIDLNEEETCT AQVLKNVFDKSSCVLGGSQE DEDVEIKFHTTKLSRAIHHF RLALQGVFQKLENNGSISPE DLESNESGSQSENSDRLLWT VSSGGAHDCSVESPASQGSE SLLSVVSGGVGISVQGDQTP QAPSNFSLASNNSPLTNSLL SFPLAPGLGNETCSRPDSPN QGKLSLEQVCAETIYLNKCI NNFKNVLREKRLRQKKLLQE LVQTASHLSVEDIPSEGKRE ALQ in isoform 2. 1 PublicationVSP_011384Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24071 mRNA. Translation: AAC52267.1.
AF159706 mRNA. Translation: AAD41910.1.
PIRiI61776.
RefSeqiNP_001036044.1. NM_001042579.1. [Q62769-1]
NP_074053.1. NM_022862.1. [Q62769-2]
UniGeneiRn.10127.

Genome annotation databases

GeneIDi64830.
KEGGirno:64830.
UCSCiRGD:619723. rat. [Q62769-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24071 mRNA. Translation: AAC52267.1.
AF159706 mRNA. Translation: AAD41910.1.
PIRiI61776.
RefSeqiNP_001036044.1. NM_001042579.1. [Q62769-1]
NP_074053.1. NM_022862.1. [Q62769-2]
UniGeneiRn.10127.

3D structure databases

ProteinModelPortaliQ62769.
SMRiQ62769. Positions 1-150, 491-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058802.

Proteomic databases

PaxDbiQ62769.
PRIDEiQ62769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64830.
KEGGirno:64830.
UCSCiRGD:619723. rat. [Q62769-1]

Organism-specific databases

CTDi10497.
RGDi619723. Unc13b.

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
HOGENOMiHOG000231404.
HOVERGENiHBG057340.
InParanoidiQ62769.
KOiK15293.

Miscellaneous databases

PROiQ62769.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 2 hits.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mammalian homologues of Caenorhabditis elegans unc-13 gene define novel family of C2-domain proteins."
    Brose N., Hofmann K., Hata Y., Suedhof T.C.
    J. Biol. Chem. 270:25273-25280(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming."
    Betz A., Thakur P., Junge H.J., Ashery U., Rhee J.S., Scheuss V., Rosenmund C., Rettig J., Brose N.
    Neuron 30:183-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH RIMS1.
    Strain: Sprague-Dawley.
  3. "Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release."
    Betz A., Ashery U., Rickmann M., Augustin I., Neher E., Suedhof T.C., Rettig J., Brose N.
    Neuron 21:123-136(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Differential expression of two novel Munc13 proteins in rat brain."
    Augustin I., Betz A., Herrmann C., Jo T., Brose N.
    Biochem. J. 337:363-371(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiUN13B_RAT
AccessioniPrimary (citable) accession number: Q62769
Secondary accession number(s): Q9WV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 6, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.