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Q62767

- DUS4_RAT

UniProt

Q62767 - DUS4_RAT

Protein

Dual specificity protein phosphatase 4

Gene

Dusp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei281 – 2811Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: RGD
    2. protein tyrosine/threonine phosphatase activity Source: RGD
    3. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. endoderm formation Source: RefGenome
    2. inactivation of MAPK activity Source: GOC
    3. protein dephosphorylation Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 4 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    Mitogen-activated protein kinase phosphatase 2
    Short name:
    MAP kinase phosphatase 2
    Short name:
    MKP-2
    Gene namesi
    Name:Dusp4
    Synonyms:Mkp2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620625. Dusp4.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Dual specificity protein phosphatase 4PRO_0000094800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei387 – 3871Phosphoserine; by MAPKBy similarity
    Modified residuei392 – 3921Phosphoserine; by MAPKBy similarity

    Post-translational modificationi

    Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ62767.
    PRIDEiQ62767.

    PTM databases

    PhosphoSiteiQ62767.

    Expressioni

    Tissue specificityi

    Expressed at moderate levels in nearly all tissues and cells including brain, spleen, and testes with the higher expression in the heart and lung and lower expression in skeletal muscle and kidney. Undetectable in liver. Expressed in many areas of the brain with very strong expression in the hippocampus, piriform cortex, and the suprachiasmatic nucleus.

    Inductioni

    By mitogens and by stress.

    Gene expression databases

    GenevestigatoriQ62767.

    Interactioni

    Subunit structurei

    Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016328.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62767.
    SMRiQ62767. Positions 194-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 160119RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini198 – 395198Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2453.
    HOGENOMiHOG000294080.
    HOVERGENiHBG007347.
    InParanoidiQ62767.
    KOiK04459.
    PhylomeDBiQ62767.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSiPR01764. MAPKPHPHTASE.
    SMARTiSM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62767-1 [UniParc]FASTAAdd to Basket

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    MVTMEELREM DCSVLKRLMN RDENGGTAGS SGGSHGALGL LSGGKCLLLD    50
    CRPFLAHSAG YIRGSVNVRC NTIVRRRAKG SVSLEQILPA EEEVRARLRS 100
    GLYSAVIVYD ERSPRAESLR EDSTVSLVVQ ALRRNAERTD ICLLKGGYER 150
    FSSEYPEFCS KTKALAAIPP PVPPSTNESL DLGCSSCGTP LHDQGGPVEI 200
    LPFLYLGSAY HAARRDMLDA LGITALLNVS SDCPNHFEGH YQYKCIPVED 250
    NHKADISSWF MEAIEYIDAV KDCRGRVLVH CQAGISRSAT ICLAYLMMKK 300
    RVRLEEAFEF VKQRRSIISP NFSFMGQLLQ FESQVLTTSC AAEAASPSGP 350
    LRERGKATPT PTSQFVFSFP VSVGVHAAPS NLPYLHSPIT TSPSC 395
    Length:395
    Mass (Da):43,187
    Last modified:November 1, 1996 - v1
    Checksum:iA90EFFD378A050FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23438 mRNA. Translation: AAC52493.1.
    RefSeqiNP_071535.1. NM_022199.1.
    UniGeneiRn.44407.

    Genome annotation databases

    GeneIDi60587.
    KEGGirno:60587.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23438 mRNA. Translation: AAC52493.1 .
    RefSeqi NP_071535.1. NM_022199.1.
    UniGenei Rn.44407.

    3D structure databases

    ProteinModelPortali Q62767.
    SMRi Q62767. Positions 194-336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000016328.

    PTM databases

    PhosphoSitei Q62767.

    Proteomic databases

    PaxDbi Q62767.
    PRIDEi Q62767.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 60587.
    KEGGi rno:60587.

    Organism-specific databases

    CTDi 1846.
    RGDi 620625. Dusp4.

    Phylogenomic databases

    eggNOGi COG2453.
    HOGENOMi HOG000294080.
    HOVERGENi HBG007347.
    InParanoidi Q62767.
    KOi K04459.
    PhylomeDBi Q62767.

    Miscellaneous databases

    NextBioi 612322.
    PROi Q62767.

    Gene expression databases

    Genevestigatori Q62767.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSi PR01764. MAPKPHPHTASE.
    SMARTi SM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel mitogen-activated protein kinase phosphatase. Structure, expression, and regulation."
      Misra-Press A., Rim C.S., Yao H., Roberson M.S., Stork P.J.S.
      J. Biol. Chem. 270:14587-14596(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pheochromocytoma.

    Entry informationi

    Entry nameiDUS4_RAT
    AccessioniPrimary (citable) accession number: Q62767
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3