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Protein

Dual specificity protein phosphatase 4

Gene

Dusp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei281Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 4 (EC:3.1.3.16, EC:3.1.3.481 Publication)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 2
Short name:
MAP kinase phosphatase 2
Short name:
MKP-2
Gene namesi
Name:Dusp4
Synonyms:Mkp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620625. Dusp4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000948002 – 395Dual specificity protein phosphatase 4Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylvalineBy similarity1
Modified residuei387Phosphoserine; by MAPKBy similarity1
Modified residuei392Phosphoserine; by MAPKBy similarity1

Post-translational modificationi

Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62767.
PRIDEiQ62767.

PTM databases

iPTMnetiQ62767.
PhosphoSitePlusiQ62767.

Expressioni

Tissue specificityi

Expressed at moderate levels in nearly all tissues and cells including brain, spleen, and testes with the higher expression in the heart and lung and lower expression in skeletal muscle and kidney. Undetectable in liver. Expressed in many areas of the brain with very strong expression in the hippocampus, piriform cortex, and the suprachiasmatic nucleus.

Inductioni

By mitogens and by stress.

Interactioni

Subunit structurei

Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016328.

Structurei

3D structure databases

ProteinModelPortaliQ62767.
SMRiQ62767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 160RhodanesePROSITE-ProRule annotationAdd BLAST119
Domaini198 – 395Tyrosine-protein phosphataseAdd BLAST198

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
HOGENOMiHOG000294080.
HOVERGENiHBG007347.
InParanoidiQ62767.
KOiK04459.
PhylomeDBiQ62767.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiView protein in InterPro
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR036873. Rhodanese-like_dom_sf.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiView protein in Pfam
PF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiView protein in SMART
SM00195. DSPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
SM00450. RHOD. 1 hit.
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTMEELREM DCSVLKRLMN RDENGGTAGS SGGSHGALGL LSGGKCLLLD
60 70 80 90 100
CRPFLAHSAG YIRGSVNVRC NTIVRRRAKG SVSLEQILPA EEEVRARLRS
110 120 130 140 150
GLYSAVIVYD ERSPRAESLR EDSTVSLVVQ ALRRNAERTD ICLLKGGYER
160 170 180 190 200
FSSEYPEFCS KTKALAAIPP PVPPSTNESL DLGCSSCGTP LHDQGGPVEI
210 220 230 240 250
LPFLYLGSAY HAARRDMLDA LGITALLNVS SDCPNHFEGH YQYKCIPVED
260 270 280 290 300
NHKADISSWF MEAIEYIDAV KDCRGRVLVH CQAGISRSAT ICLAYLMMKK
310 320 330 340 350
RVRLEEAFEF VKQRRSIISP NFSFMGQLLQ FESQVLTTSC AAEAASPSGP
360 370 380 390
LRERGKATPT PTSQFVFSFP VSVGVHAAPS NLPYLHSPIT TSPSC
Length:395
Mass (Da):43,187
Last modified:November 1, 1996 - v1
Checksum:iA90EFFD378A050FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23438 mRNA. Translation: AAC52493.1.
RefSeqiNP_071535.1. NM_022199.1.
UniGeneiRn.44407.

Genome annotation databases

GeneIDi60587.
KEGGirno:60587.

Similar proteinsi

Entry informationi

Entry nameiDUS4_RAT
AccessioniPrimary (citable) accession number: Q62767
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families