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Q62762 (KC1G2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Casein kinase I isoform gamma-2

Short name=CKI-gamma 2
EC=2.7.11.1
Gene names
Name:Csnk1g2
Synonyms:Ck1g2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling By similarity. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Stimulated by estrogen By similarity.

Subunit structure

Monomer. Interacts with MTA1 (short isoform) in the cytoplasm. Interacts with SMAD3 By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Testis.

Post-translational modification

Autophosphorylated.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Casein kinase I isoform gamma-2
PRO_0000192844

Regions

Domain46 – 316271Protein kinase
Nucleotide binding52 – 609ATP By similarity

Sites

Active site1651Proton acceptor By similarity
Binding site751ATP By similarity

Experimental info

Sequence conflict59 – 602EL → DV in AAC52201. Ref.1
Sequence conflict1001Missing in AAC52201. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q62762 [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: 6BE480CE200FBEBB

FASTA41547,636
        10         20         30         40         50         60 
MDFDKKGGKG ELEEGRRMSK TGTNRSNHGV RNSGTSSGVL MVGPNFRVGK KIGCGNFGEL 

        70         80         90        100        110        120 
RLGKNLYTNE YVAIKLEPIK SRAPQLHLEY RFYKQLSTTE GVPQVYYFGP CGKYNAMVLE 

       130        140        150        160        170        180 
LLGPSLEDLF DLCDRTFTLK TVLMIAIQLI TRMEYVHTKS LIYRDVKPEN FLVGRPGSKR 

       190        200        210        220        230        240 
QHSIHIIDFG LAKEYIDPET KKHIPYREHK SLTGTARYMS INTHLGKEQS RRDDLEALGH 

       250        260        270        280        290        300 
MFMYFLRGSL PWQGLKADTL KERYQKIGDT KRATPIEVLC ESFPEEMATY LRYVRRLDFF 

       310        320        330        340        350        360 
EKPDYDYLRK LFTDLFDRSG YVFDYEYDWA GKPLPTPIGT VHPDVPSQPP HRDKAQLHTK 

       370        380        390        400        410 
NQALNSTNGE LNTDDPTAGH SNAPIAAPAE VEVADETKCC CFFKRRKRKS LQRHK 

« Hide

References

« Hide 'large scale' references
[1]"Casein kinase I gamma subfamily. Molecular cloning, expression, and characterization of three mammalian isoforms and complementation of defects in the Saccharomyces cerevisiae YCK genes."
Zhai L., Graves P.R., Robinson L.C., Italiano M., Culbertson M.R., Rowles J., Cobb M.H., Depaoli-Roach A.A., Roach P.J.
J. Biol. Chem. 270:12717-12724(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22297 mRNA. Translation: AAC52201.1.
BC072533 mRNA. Translation: AAH72533.1.
PIRB56711.
RefSeqNP_001029042.1. NM_001033870.1.
NP_075590.2. NM_023102.2.
XP_006241064.1. XM_006241002.1.
UniGeneRn.98151.

3D structure databases

ProteinModelPortalQ62762.
SMRQ62762. Positions 39-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000022473.

Proteomic databases

PaxDbQ62762.
PRIDEQ62762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025266; ENSRNOP00000025266; ENSRNOG00000018529.
GeneID65278.
KEGGrno:65278.
UCSCRGD:621407. rat.

Organism-specific databases

CTD1455.
RGD621407. Csnk1g2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110718.
HOGENOMHOG000182054.
HOVERGENHBG000176.
KOK08958.
OMAHRNTHEP.
OrthoDBEOG7G1V66.
PhylomeDBQ62762.

Enzyme and pathway databases

BRENDA2.7.11.1. 5301.

Gene expression databases

ArrayExpressQ62762.
GenevestigatorQ62762.

Family and domain databases

InterProIPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF12605. CK1gamma_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614248.

Entry information

Entry nameKC1G2_RAT
AccessionPrimary (citable) accession number: Q62762
Secondary accession number(s): Q6IMY5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families