SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q62760

- TOM20_RAT

UniProt

Q62760 - TOM20_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Mitochondrial import receptor subunit TOM20 homolog
Gene
Tomm20
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore By similarity.

GO - Molecular functioni

  1. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: RGD
  2. unfolded protein binding Source: RGD
Complete GO annotation...

GO - Biological processi

  1. protein targeting to mitochondrion Source: HGNC
  2. protein transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199211. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20 homolog
Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Outer mitochondrial membrane receptor Tom20
Gene namesi
Name:Tomm20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi708467. Tomm20.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Mitochondrial intermembrane Reviewed prediction
Transmembranei7 – 2418Helical; Reviewed prediction
Add
BLAST
Topological domaini25 – 145121Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: UniProtKB
  3. mitochondrial outer membrane translocase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Mitochondrial import receptor subunit TOM20 homolog
PRO_0000051541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki56 – 56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki68 – 68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei135 – 1351Phosphoserine By similarity
Modified residuei138 – 1381Phosphoserine By similarity

Post-translational modificationi

Ubiquitinated at Lys-56, Lys-61 and Lys-68 by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30 By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62760.
PRIDEiQ62760.

PTM databases

PhosphoSiteiQ62760.

Expressioni

Gene expression databases

GenevestigatoriQ62760.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1 By similarity.

Protein-protein interaction databases

BioGridi251753. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 583
Helixi59 – 8224
Helixi86 – 9813
Helixi104 – 11310
Helixi116 – 12510
Helixi129 – 1346
Turni135 – 1406

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-A51-145[»]
2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
2V1TX-ray1.92A/B59-126[»]
3AWRX-ray2.00A/B59-126[»]
3AX2X-ray1.90A/C/E/G59-126[»]
3AX3X-ray2.10A/C/E/G59-126[»]
3AX5X-ray2.20A/C59-126[»]
ProteinModelPortaliQ62760.
SMRiQ62760. Positions 59-126.

Miscellaneous databases

EvolutionaryTraceiQ62760.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tom20 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307355.
GeneTreeiENSGT00390000011698.
HOGENOMiHOG000006671.
HOVERGENiHBG057315.
InParanoidiQ6AZ66.
KOiK17770.
OMAiKMVGRNS.
OrthoDBiEOG790G2R.
PhylomeDBiQ62760.
TreeFamiTF106200.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20_rcpt-related.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62760-1 [UniParc]FASTAAdd to Basket

« Hide

MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE    50
RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC 100
GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLA EDDVE 145
Length:145
Mass (Da):16,284
Last modified:February 5, 2008 - v2
Checksum:i50E3BD25AD5C9B3B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121V → L in AAB01506. 1 Publication
Sequence conflicti30 – 301S → G in AAB01506. 1 Publication
Sequence conflicti36 – 361N → D in BAA09714. 1 Publication
Sequence conflicti140 – 1401A → G in BAA09714. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21871 mRNA. Translation: AAB01506.1.
D63411 mRNA. Translation: BAA09714.1.
BC078715 mRNA. Translation: AAH78715.1.
RefSeqiNP_690918.1. NM_152935.1.
UniGeneiRn.2143.

Genome annotation databases

EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
GeneIDi266601.
KEGGirno:266601.
UCSCiRGD:708467. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21871 mRNA. Translation: AAB01506.1 .
D63411 mRNA. Translation: BAA09714.1 .
BC078715 mRNA. Translation: AAH78715.1 .
RefSeqi NP_690918.1. NM_152935.1.
UniGenei Rn.2143.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OM2 NMR - A 51-145 [» ]
2V1S X-ray 2.05 A/B/C/D/E/F/G 59-126 [» ]
2V1T X-ray 1.92 A/B 59-126 [» ]
3AWR X-ray 2.00 A/B 59-126 [» ]
3AX2 X-ray 1.90 A/C/E/G 59-126 [» ]
3AX3 X-ray 2.10 A/C/E/G 59-126 [» ]
3AX5 X-ray 2.20 A/C 59-126 [» ]
ProteinModelPortali Q62760.
SMRi Q62760. Positions 59-126.
ModBasei Search...

Protein-protein interaction databases

BioGridi 251753. 1 interaction.

PTM databases

PhosphoSitei Q62760.

Proteomic databases

PaxDbi Q62760.
PRIDEi Q62760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027088 ; ENSRNOP00000027088 ; ENSRNOG00000019980 .
GeneIDi 266601.
KEGGi rno:266601.
UCSCi RGD:708467. rat.

Organism-specific databases

CTDi 9804.
RGDi 708467. Tomm20.

Phylogenomic databases

eggNOGi NOG307355.
GeneTreei ENSGT00390000011698.
HOGENOMi HOG000006671.
HOVERGENi HBG057315.
InParanoidi Q6AZ66.
KOi K17770.
OMAi KMVGRNS.
OrthoDBi EOG790G2R.
PhylomeDBi Q62760.
TreeFami TF106200.

Enzyme and pathway databases

Reactomei REACT_199211. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTracei Q62760.
NextBioi 624408.
PROi Q62760.

Gene expression databases

Genevestigatori Q62760.

Family and domain databases

Gene3Di 1.20.960.10. 1 hit.
InterProi IPR002056. MAS20_rcpt-related.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view ]
PANTHERi PTHR12430. PTHR12430. 1 hit.
Pfami PF02064. MAS20. 1 hit.
[Graphical view ]
PIRSFi PIRSF037707. MAS20_rcpt. 1 hit.
PRINTSi PR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMi SSF47157. SSF47157. 1 hit.
TIGRFAMsi TIGR00985. 3a0801s04tom. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Nuttall S.D., Sinding J., Hanson B., Hoogenraad N.J.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "cDNA cloning and characterization of rat mitochondrial precursor receptor."
    Mihara K., Nomura N., Matsuo H., Sakaguchi M.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20."
    Abe Y., Shodai T., Muto T., Mihara K., Torii H., Nishikawa S., Endo T., Kohda D.
    Cell 100:551-560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 51-145.

Entry informationi

Entry nameiTOM20_RAT
AccessioniPrimary (citable) accession number: Q62760
Secondary accession number(s): O08517, Q63804, Q6AZ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi