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Q62760

- TOM20_RAT

UniProt

Q62760 - TOM20_RAT

Protein

Mitochondrial import receptor subunit TOM20 homolog

Gene

Tomm20

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore By similarity.By similarity

    GO - Molecular functioni

    1. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: RGD
    2. unfolded protein binding Source: RGD

    GO - Biological processi

    1. protein targeting to mitochondrion Source: HGNC
    2. protein transmembrane transport Source: GOC

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199211. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import receptor subunit TOM20 homolog
    Alternative name(s):
    Mitochondrial 20 kDa outer membrane protein
    Outer mitochondrial membrane receptor Tom20
    Gene namesi
    Name:Tomm20
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi708467. Tomm20.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: UniProtKB
    3. mitochondrial outer membrane translocase complex Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 145145Mitochondrial import receptor subunit TOM20 homologPRO_0000051541Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki56 – 56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki68 – 68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei135 – 1351PhosphoserineBy similarity
    Modified residuei138 – 1381PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated at Lys-56, Lys-61 and Lys-68 by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30 By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ62760.
    PRIDEiQ62760.

    PTM databases

    PhosphoSiteiQ62760.

    Expressioni

    Gene expression databases

    GenevestigatoriQ62760.

    Interactioni

    Subunit structurei

    Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi251753. 1 interaction.

    Structurei

    Secondary structure

    1
    145
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi56 – 583
    Helixi59 – 8224
    Helixi86 – 9813
    Helixi104 – 11310
    Helixi116 – 12510
    Helixi129 – 1346
    Turni135 – 1406

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OM2NMR-A51-145[»]
    2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
    2V1TX-ray1.92A/B59-126[»]
    3AWRX-ray2.00A/B59-126[»]
    3AX2X-ray1.90A/C/E/G59-126[»]
    3AX3X-ray2.10A/C/E/G59-126[»]
    3AX5X-ray2.20A/C59-126[»]
    ProteinModelPortaliQ62760.
    SMRiQ62760. Positions 59-126.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62760.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66Mitochondrial intermembraneSequence Analysis
    Topological domaini25 – 145121CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2418HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Tom20 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG307355.
    GeneTreeiENSGT00390000011698.
    HOGENOMiHOG000006671.
    HOVERGENiHBG057315.
    InParanoidiQ6AZ66.
    KOiK17770.
    OMAiKMVGRNS.
    OrthoDBiEOG790G2R.
    PhylomeDBiQ62760.
    TreeFamiTF106200.

    Family and domain databases

    Gene3Di1.20.960.10. 1 hit.
    InterProiIPR002056. MAS20_rcpt-related.
    IPR022422. MAS20_rcpt_metazoan.
    IPR023392. Tom20_dom.
    [Graphical view]
    PANTHERiPTHR12430. PTHR12430. 1 hit.
    PfamiPF02064. MAS20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
    PRINTSiPR01989. EUOM20RECPTR.
    PR00351. OM20RECEPTOR.
    SUPFAMiSSF47157. SSF47157. 1 hit.
    TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q62760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE    50
    RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC 100
    GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLA EDDVE 145
    Length:145
    Mass (Da):16,284
    Last modified:February 5, 2008 - v2
    Checksum:i50E3BD25AD5C9B3B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121V → L in AAB01506. 1 PublicationCurated
    Sequence conflicti30 – 301S → G in AAB01506. 1 PublicationCurated
    Sequence conflicti36 – 361N → D in BAA09714. 1 PublicationCurated
    Sequence conflicti140 – 1401A → G in BAA09714. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21871 mRNA. Translation: AAB01506.1.
    D63411 mRNA. Translation: BAA09714.1.
    BC078715 mRNA. Translation: AAH78715.1.
    RefSeqiNP_690918.1. NM_152935.1.
    UniGeneiRn.2143.

    Genome annotation databases

    EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
    GeneIDi266601.
    KEGGirno:266601.
    UCSCiRGD:708467. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21871 mRNA. Translation: AAB01506.1 .
    D63411 mRNA. Translation: BAA09714.1 .
    BC078715 mRNA. Translation: AAH78715.1 .
    RefSeqi NP_690918.1. NM_152935.1.
    UniGenei Rn.2143.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OM2 NMR - A 51-145 [» ]
    2V1S X-ray 2.05 A/B/C/D/E/F/G 59-126 [» ]
    2V1T X-ray 1.92 A/B 59-126 [» ]
    3AWR X-ray 2.00 A/B 59-126 [» ]
    3AX2 X-ray 1.90 A/C/E/G 59-126 [» ]
    3AX3 X-ray 2.10 A/C/E/G 59-126 [» ]
    3AX5 X-ray 2.20 A/C 59-126 [» ]
    ProteinModelPortali Q62760.
    SMRi Q62760. Positions 59-126.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251753. 1 interaction.

    PTM databases

    PhosphoSitei Q62760.

    Proteomic databases

    PaxDbi Q62760.
    PRIDEi Q62760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027088 ; ENSRNOP00000027088 ; ENSRNOG00000019980 .
    GeneIDi 266601.
    KEGGi rno:266601.
    UCSCi RGD:708467. rat.

    Organism-specific databases

    CTDi 9804.
    RGDi 708467. Tomm20.

    Phylogenomic databases

    eggNOGi NOG307355.
    GeneTreei ENSGT00390000011698.
    HOGENOMi HOG000006671.
    HOVERGENi HBG057315.
    InParanoidi Q6AZ66.
    KOi K17770.
    OMAi KMVGRNS.
    OrthoDBi EOG790G2R.
    PhylomeDBi Q62760.
    TreeFami TF106200.

    Enzyme and pathway databases

    Reactomei REACT_199211. Mitochondrial protein import.

    Miscellaneous databases

    EvolutionaryTracei Q62760.
    NextBioi 624408.
    PROi Q62760.

    Gene expression databases

    Genevestigatori Q62760.

    Family and domain databases

    Gene3Di 1.20.960.10. 1 hit.
    InterProi IPR002056. MAS20_rcpt-related.
    IPR022422. MAS20_rcpt_metazoan.
    IPR023392. Tom20_dom.
    [Graphical view ]
    PANTHERi PTHR12430. PTHR12430. 1 hit.
    Pfami PF02064. MAS20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037707. MAS20_rcpt. 1 hit.
    PRINTSi PR01989. EUOM20RECPTR.
    PR00351. OM20RECEPTOR.
    SUPFAMi SSF47157. SSF47157. 1 hit.
    TIGRFAMsi TIGR00985. 3a0801s04tom. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Nuttall S.D., Sinding J., Hanson B., Hoogenraad N.J.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA cloning and characterization of rat mitochondrial precursor receptor."
      Mihara K., Nomura N., Matsuo H., Sakaguchi M.
      Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20."
      Abe Y., Shodai T., Muto T., Mihara K., Torii H., Nishikawa S., Endo T., Kohda D.
      Cell 100:551-560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 51-145.

    Entry informationi

    Entry nameiTOM20_RAT
    AccessioniPrimary (citable) accession number: Q62760
    Secondary accession number(s): O08517, Q63804, Q6AZ66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3