Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial import receptor subunit TOM20 homolog

Gene

Tomm20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity).By similarity

GO - Molecular functioni

  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: RGD
  • unfolded protein binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_294109. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20 homolog
Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Outer mitochondrial membrane receptor Tom20
Gene namesi
Name:Tomm20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi708467. Tomm20.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Mitochondrial intermembraneSequence Analysis
Transmembranei7 – 2418HelicalSequence AnalysisAdd
BLAST
Topological domaini25 – 145121CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • ER-mitochondrion membrane contact site Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial outer membrane Source: UniProtKB
  • mitochondrial outer membrane translocase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Mitochondrial import receptor subunit TOM20 homologPRO_0000051541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki35 – 35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki56 – 56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki68 – 68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei135 – 1351PhosphoserineBy similarity
Modified residuei138 – 1381PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62760.
PRIDEiQ62760.

PTM databases

PhosphoSiteiQ62760.

Expressioni

Gene expression databases

GenevisibleiQ62760. RN.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi251753. 1 interaction.
STRINGi10116.ENSRNOP00000027088.

Structurei

Secondary structure

1
145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 583Combined sources
Helixi59 – 8224Combined sources
Helixi86 – 9813Combined sources
Helixi104 – 11310Combined sources
Helixi116 – 12510Combined sources
Helixi129 – 1346Combined sources
Turni135 – 1406Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-A51-145[»]
2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
2V1TX-ray1.92A/B59-126[»]
3AWRX-ray2.00A/B59-126[»]
3AX2X-ray1.90A/C/E/G59-126[»]
3AX3X-ray2.10A/C/E/G59-126[»]
3AX5X-ray2.20A/C59-126[»]
ProteinModelPortaliQ62760.
SMRiQ62760. Positions 59-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62760.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tom20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307355.
GeneTreeiENSGT00390000011698.
HOGENOMiHOG000006671.
HOVERGENiHBG057315.
InParanoidiQ62760.
KOiK17770.
OMAiAMLIIKM.
OrthoDBiEOG790G2R.
PhylomeDBiQ62760.
TreeFamiTF106200.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE
60 70 80 90 100
RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC
110 120 130 140
GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLA EDDVE
Length:145
Mass (Da):16,284
Last modified:February 5, 2008 - v2
Checksum:i50E3BD25AD5C9B3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121V → L in AAB01506 (Ref. 1) Curated
Sequence conflicti30 – 301S → G in AAB01506 (Ref. 1) Curated
Sequence conflicti36 – 361N → D in BAA09714 (Ref. 2) Curated
Sequence conflicti140 – 1401A → G in BAA09714 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21871 mRNA. Translation: AAB01506.1.
D63411 mRNA. Translation: BAA09714.1.
BC078715 mRNA. Translation: AAH78715.1.
RefSeqiNP_690918.1. NM_152935.1.
UniGeneiRn.2143.

Genome annotation databases

EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
GeneIDi266601.
KEGGirno:266601.
UCSCiRGD:708467. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21871 mRNA. Translation: AAB01506.1.
D63411 mRNA. Translation: BAA09714.1.
BC078715 mRNA. Translation: AAH78715.1.
RefSeqiNP_690918.1. NM_152935.1.
UniGeneiRn.2143.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-A51-145[»]
2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
2V1TX-ray1.92A/B59-126[»]
3AWRX-ray2.00A/B59-126[»]
3AX2X-ray1.90A/C/E/G59-126[»]
3AX3X-ray2.10A/C/E/G59-126[»]
3AX5X-ray2.20A/C59-126[»]
ProteinModelPortaliQ62760.
SMRiQ62760. Positions 59-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251753. 1 interaction.
STRINGi10116.ENSRNOP00000027088.

PTM databases

PhosphoSiteiQ62760.

Proteomic databases

PaxDbiQ62760.
PRIDEiQ62760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
GeneIDi266601.
KEGGirno:266601.
UCSCiRGD:708467. rat.

Organism-specific databases

CTDi9804.
RGDi708467. Tomm20.

Phylogenomic databases

eggNOGiNOG307355.
GeneTreeiENSGT00390000011698.
HOGENOMiHOG000006671.
HOVERGENiHBG057315.
InParanoidiQ62760.
KOiK17770.
OMAiAMLIIKM.
OrthoDBiEOG790G2R.
PhylomeDBiQ62760.
TreeFamiTF106200.

Enzyme and pathway databases

ReactomeiREACT_294109. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiQ62760.
NextBioi624408.
PROiQ62760.

Gene expression databases

GenevisibleiQ62760. RN.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Nuttall S.D., Sinding J., Hanson B., Hoogenraad N.J.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "cDNA cloning and characterization of rat mitochondrial precursor receptor."
    Mihara K., Nomura N., Matsuo H., Sakaguchi M.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20."
    Abe Y., Shodai T., Muto T., Mihara K., Torii H., Nishikawa S., Endo T., Kohda D.
    Cell 100:551-560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 51-145.

Entry informationi

Entry nameiTOM20_RAT
AccessioniPrimary (citable) accession number: Q62760
Secondary accession number(s): O08517, Q63804, Q6AZ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: July 22, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.