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Protein

Mitochondrial import receptor subunit TOM20 homolog

Gene

Tomm20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity).By similarity

GO - Molecular functioni

  • mitochondrion targeting sequence binding Source: GO_Central
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: RGD
  • unfolded protein binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20 homolog
Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Outer mitochondrial membrane receptor Tom20
Gene namesi
Name:Tomm20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi708467. Tomm20.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6Mitochondrial intermembraneSequence analysis6
Transmembranei7 – 24HelicalSequence analysisAdd BLAST18
Topological domaini25 – 145CytoplasmicSequence analysisAdd BLAST121

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515411 – 145Mitochondrial import receptor subunit TOM20 homologAdd BLAST145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei135PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62760.
PRIDEiQ62760.

PTM databases

iPTMnetiQ62760.
PhosphoSitePlusiQ62760.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019980.
GenevisibleiQ62760. RN.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1 (By similarity).By similarity

GO - Molecular functioni

  • unfolded protein binding Source: RGD

Protein-protein interaction databases

BioGridi251753. 1 interactor.
STRINGi10116.ENSRNOP00000027088.

Structurei

Secondary structure

1145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 58Combined sources3
Beta strandi60 – 62Combined sources3
Helixi65 – 82Combined sources18
Helixi86 – 98Combined sources13
Helixi104 – 113Combined sources10
Helixi116 – 123Combined sources8
Helixi129 – 134Combined sources6
Turni135 – 140Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-A51-145[»]
2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
2V1TX-ray1.92A/B59-126[»]
3AWRX-ray2.00A/B59-126[»]
3AX2X-ray1.90A/C/E/G59-126[»]
3AX3X-ray2.10A/C/E/G59-126[»]
3AX5X-ray2.20A/C59-126[»]
5AZ6X-ray2.56A/B65-126[»]
5AZ7X-ray1.96A65-126[»]
5AZ8X-ray1.70A65-126[»]
5AZ9X-ray1.82A65-126[»]
ProteinModelPortaliQ62760.
SMRiQ62760.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62760.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tom20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4056. Eukaryota.
ENOG4111NAH. LUCA.
GeneTreeiENSGT00390000011698.
HOGENOMiHOG000006671.
HOVERGENiHBG057315.
InParanoidiQ62760.
KOiK17770.
OMAiAMLIIKM.
OrthoDBiEOG091G0Z80.
PhylomeDBiQ62760.
TreeFamiTF106200.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE
60 70 80 90 100
RAGLSKLPDL KDAEAVQKFF LEEIQLGEEL LAQGDYEKGV DHLTNAIAVC
110 120 130 140
GQPQQLLQVL QQTLPPPVFQ MLLTKLPTIS QRIVSAQSLA EDDVE
Length:145
Mass (Da):16,284
Last modified:February 5, 2008 - v2
Checksum:i50E3BD25AD5C9B3B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12V → L in AAB01506 (Ref. 1) Curated1
Sequence conflicti30S → G in AAB01506 (Ref. 1) Curated1
Sequence conflicti36N → D in BAA09714 (Ref. 2) Curated1
Sequence conflicti140A → G in BAA09714 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21871 mRNA. Translation: AAB01506.1.
D63411 mRNA. Translation: BAA09714.1.
BC078715 mRNA. Translation: AAH78715.1.
RefSeqiNP_690918.1. NM_152935.1.
UniGeneiRn.2143.

Genome annotation databases

EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
GeneIDi266601.
KEGGirno:266601.
UCSCiRGD:708467. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21871 mRNA. Translation: AAB01506.1.
D63411 mRNA. Translation: BAA09714.1.
BC078715 mRNA. Translation: AAH78715.1.
RefSeqiNP_690918.1. NM_152935.1.
UniGeneiRn.2143.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-A51-145[»]
2V1SX-ray2.05A/B/C/D/E/F/G59-126[»]
2V1TX-ray1.92A/B59-126[»]
3AWRX-ray2.00A/B59-126[»]
3AX2X-ray1.90A/C/E/G59-126[»]
3AX3X-ray2.10A/C/E/G59-126[»]
3AX5X-ray2.20A/C59-126[»]
5AZ6X-ray2.56A/B65-126[»]
5AZ7X-ray1.96A65-126[»]
5AZ8X-ray1.70A65-126[»]
5AZ9X-ray1.82A65-126[»]
ProteinModelPortaliQ62760.
SMRiQ62760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251753. 1 interactor.
STRINGi10116.ENSRNOP00000027088.

PTM databases

iPTMnetiQ62760.
PhosphoSitePlusiQ62760.

Proteomic databases

PaxDbiQ62760.
PRIDEiQ62760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027088; ENSRNOP00000027088; ENSRNOG00000019980.
GeneIDi266601.
KEGGirno:266601.
UCSCiRGD:708467. rat.

Organism-specific databases

CTDi9804.
RGDi708467. Tomm20.

Phylogenomic databases

eggNOGiKOG4056. Eukaryota.
ENOG4111NAH. LUCA.
GeneTreeiENSGT00390000011698.
HOGENOMiHOG000006671.
HOVERGENiHBG057315.
InParanoidiQ62760.
KOiK17770.
OMAiAMLIIKM.
OrthoDBiEOG091G0Z80.
PhylomeDBiQ62760.
TreeFamiTF106200.

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Miscellaneous databases

EvolutionaryTraceiQ62760.
PROiQ62760.

Gene expression databases

BgeeiENSRNOG00000019980.
GenevisibleiQ62760. RN.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20.
IPR022422. MAS20_rcpt_metazoan.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR01989. EUOM20RECPTR.
PR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOM20_RAT
AccessioniPrimary (citable) accession number: Q62760
Secondary accession number(s): O08517, Q63804, Q6AZ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.