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Protein

Rab-3A-interacting protein

Gene

Rab3ip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity).By similarity

GO - Molecular functioni

  1. GTPase binding Source: RGD
  2. Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  1. positive regulation of Rab GTPase activity Source: GOC
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Rab-3A-interacting protein
Short name:
Rab3A-interacting protein
Alternative name(s):
Rabin-3
SSX2-interacting protein
Gene namesi
Name:Rab3ipBy similarity
Synonyms:Rabin3, Rabin8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620650. Rab3ip.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionlamellipodium By similarity
Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin (By similarity).By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-SubCell
  2. cytosol Source: UniProtKB
  3. lamellipodium Source: UniProtKB-SubCell
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Rab-3A-interacting proteinPRO_0000097146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ62739.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed at highest level in testis.1 Publication

Gene expression databases

GenevestigatoriQ62739.

Interactioni

Subunit structurei

Interacts with the N-terminal region of SSX2 (By similarity). Interacts with the GDP-bound forms of RAB3A, RAB3D, RAB8A and RAB8B.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Exoc6Q8R3132EBI-2028671,EBI-9202179From a different organism.
Rab11aP624924EBI-2028671,EBI-770256From a different organism.

Protein-protein interaction databases

IntActiQ62739. 4 interactions.
MINTiMINT-1506178.

Structurei

3D structure databases

ProteinModelPortaliQ62739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili149 – 24496Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SEC2 family.Sequence Analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG299882.
HOGENOMiHOG000220814.
HOVERGENiHBG057034.
InParanoidiQ62739.
PhylomeDBiQ62739.

Family and domain databases

InterProiIPR009449. Sec2p.
[Graphical view]
PfamiPF06428. Sec2p. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANDPLEGFH EVNLASPTSP DLLGVCDPGT QEQTTSPSVI YRPHPSTLCS
60 70 80 90 100
ATIQANALNL SDLPTQPVYS SPRHLNCAEI SNISIHVPEP ASSVASEVAA
110 120 130 140 150
GLTRFTSRKD SCNAEREFLQ GATVTEASAG NDDIFGLSTD SLSRLRSPSV
160 170 180 190 200
LEVREKGYER LKEELAKAQR ELKLKDEECE RLSKVRDQLG QELEELTASL
210 220 230 240 250
FEEAHKMVRE ANVKQATAEK QLKEAQGKID VLQAEVAALK TLVLSSSPTS
260 270 280 290 300
PTQEPLADGK TPFKGGHTRN KSTSSAMSGS HQDFSAIQAI VKDCREADLS
310 320 330 340 350
LYNEFRSWKD EPTMDRTCPF LDKIYQEDIF PCLTFAKSEL ASAVLEAVEN
360 370 380 390 400
NTLSIEPVGL QPIRFVKASA VECGGPKKCA LTGQSKPCKH RIKLGDSSSY
410 420 430 440 450
YYISPVCRYR ITSVCNFFTY IRYIQQGLVK QQDVDQMFWE VMQLRKEMSL
460
AKLGYFKEEL
Length:460
Mass (Da):50,951
Last modified:October 31, 1996 - v1
Checksum:iD590A8BDE2AEBB4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19181 mRNA. Translation: AAA67890.1.
PIRiI57546.
UniGeneiRn.31889.

Genome annotation databases

UCSCiRGD:620650. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19181 mRNA. Translation: AAA67890.1.
PIRiI57546.
UniGeneiRn.31889.

3D structure databases

ProteinModelPortaliQ62739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62739. 4 interactions.
MINTiMINT-1506178.

Proteomic databases

PRIDEiQ62739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620650. rat.

Organism-specific databases

RGDi620650. Rab3ip.

Phylogenomic databases

eggNOGiNOG299882.
HOGENOMiHOG000220814.
HOVERGENiHBG057034.
InParanoidiQ62739.
PhylomeDBiQ62739.

Miscellaneous databases

PROiQ62739.

Gene expression databases

GenevestigatoriQ62739.

Family and domain databases

InterProiIPR009449. Sec2p.
[Graphical view]
PfamiPF06428. Sec2p. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A."
    Brondyk W.H., McKiernan C.J., Fortner K.A., Stabila P., Holz R.W., Macara I.G.
    Mol. Cell. Biol. 15:1137-1143(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB3A AND RAB3D, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Fischer 344Imported.
    Tissue: BrainImported.
  2. "A Rab8-specific GDP/GTP exchange factor is involved in actin remodeling and polarized membrane transport."
    Hattula K., Furuhjelm J., Arffman A., Peranen J.
    Mol. Biol. Cell 13:3268-3280(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRAB3I_RAT
AccessioniPrimary (citable) accession number: Q62739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2005
Last sequence update: October 31, 1996
Last modified: March 3, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.