Q62739 (RAB3I_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rab-3A-interacting protein
Short name=Rab3A-interacting protein

Alternative name(s):
Rabin-3
SSX2-interacting protein

Gene names

Name:	Rab3ip
Synonyms:	Rabin3, Rabin8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis By similarity. Ref.2
Subunit structure	Interacts with the N-terminal region of SSX2 By similarity. Interacts with the GDP-bound forms of RAB3A, RAB3D, RAB8A and RAB8B. Ref.1 UniProtKB Q96QF0
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin By similarity. Ref.1
Tissue specificity	Ubiquitously expressed. Expressed at highest level in testis. Ref.1
Sequence similarities	Belongs to the SEC2 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell projection Cytoplasm Cytoskeleton Nucleus
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay Ref.1. Source: UniProtKB lamellipodium Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	Rab guanyl-nucleotide exchange factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Rab-3A-interacting protein		PRO_0000097146
Regions
	Coiled coil	149 – 244	96	Potential
Amino acid modifications
	Modified residue	147	1	Phosphoserine By similarity
	Modified residue	249	1	Phosphothreonine By similarity
	Modified residue	272	1	Phosphoserine By similarity
	Modified residue	274	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62739 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D590A8BDE2AEBB4D	FASTA	460	50,951
	10 20 30 40 50 60 MANDPLEGFH EVNLASPTSP DLLGVCDPGT QEQTTSPSVI YRPHPSTLCS ATIQANALNL 70 80 90 100 110 120 SDLPTQPVYS SPRHLNCAEI SNISIHVPEP ASSVASEVAA GLTRFTSRKD SCNAEREFLQ 130 140 150 160 170 180 GATVTEASAG NDDIFGLSTD SLSRLRSPSV LEVREKGYER LKEELAKAQR ELKLKDEECE 190 200 210 220 230 240 RLSKVRDQLG QELEELTASL FEEAHKMVRE ANVKQATAEK QLKEAQGKID VLQAEVAALK 250 260 270 280 290 300 TLVLSSSPTS PTQEPLADGK TPFKGGHTRN KSTSSAMSGS HQDFSAIQAI VKDCREADLS 310 320 330 340 350 360 LYNEFRSWKD EPTMDRTCPF LDKIYQEDIF PCLTFAKSEL ASAVLEAVEN NTLSIEPVGL 370 380 390 400 410 420 QPIRFVKASA VECGGPKKCA LTGQSKPCKH RIKLGDSSSY YYISPVCRYR ITSVCNFFTY 430 440 450 460 IRYIQQGLVK QQDVDQMFWE VMQLRKEMSL AKLGYFKEEL

References

[1]	"Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A." Brondyk W.H., McKiernan C.J., Fortner K.A., Stabila P., Holz R.W., Macara I.G. Mol. Cell. Biol. 15:1137-1143(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB3A AND RAB3D, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Fischer 344. Tissue: Brain.
[2]	"A Rab8-specific GDP/GTP exchange factor is involved in actin remodeling and polarized membrane transport." Hattula K., Furuhjelm J., Arffman A., Peranen J. Mol. Biol. Cell 13:3268-3280(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19181 mRNA. Translation: AAA67890.1.
IPI	IPI00208122.
PIR	I57546.
UniGene	Rn.31889.
3D structure databases
ProteinModelPortal	Q62739.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62739. 1 interaction.
Proteomic databases
PRIDE	Q62739.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:620650. rat.
Organism-specific databases
RGD	620650. Rab3ip.
Phylogenomic databases
eggNOG	NOG299882.
HOGENOM	HOG000220814.
HOVERGEN	HBG057034.
InParanoid	Q62739.
OrthoDB	EOG4X6C8J.
Gene expression databases
ArrayExpress	Q62739.
Genevestigator	Q62739.
GermOnline	ENSRNOG00000005362. Rattus norvegicus.
Family and domain databases
InterPro	IPR009449. Sec2p. [Graphical view]
Pfam	PF06428. Sec2p. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAB3I_RAT

Accession

Primary (citable) accession number: Q62739

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program