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Protein

Cytochrome b-245 light chain

Gene

Cyba

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: RGD
  • cellular response to angiotensin Source: RGD
  • cellular response to gamma radiation Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to L-glutamine Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to organic substance Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • cytochrome complex assembly Source: Ensembl
  • hydrogen peroxide biosynthetic process Source: BHF-UCL
  • inflammatory response Source: Ensembl
  • innate immune response Source: Ensembl
  • negative regulation of glomerular filtration by angiotensin Source: RGD
  • oxidation-reduction process Source: BHF-UCL
  • positive regulation of cell growth Source: RGD
  • positive regulation of defense response to bacterium Source: Ensembl
  • positive regulation of endothelial cell proliferation Source: RGD
  • positive regulation of interleukin-6 production Source: Ensembl
  • positive regulation of mucus secretion Source: Ensembl
  • positive regulation of NAD(P)H oxidase activity Source: RGD
  • positive regulation of phagocytosis Source: Ensembl
  • positive regulation of reactive oxygen species biosynthetic process Source: Ensembl
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of superoxide anion generation Source: RGD
  • positive regulation of toll-like receptor 2 signaling pathway Source: Ensembl
  • positive regulation of tumor necrosis factor production Source: Ensembl
  • regulation of blood pressure Source: RGD
  • regulation of release of sequestered calcium ion into cytosol Source: Ensembl
  • respiratory burst Source: Ensembl
  • response to activity Source: RGD
  • response to aldosterone Source: RGD
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to interleukin-1 Source: RGD
  • response to nutrient levels Source: RGD
  • smooth muscle hypertrophy Source: BHF-UCL
  • superoxide anion generation Source: BHF-UCL
  • superoxide metabolic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-1222556 ROS, RNS production in phagocytes
R-RNO-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-RNO-3299685 Detoxification of Reactive Oxygen Species
R-RNO-4420097 VEGFA-VEGFR2 Pathway
R-RNO-5668599 RHO GTPases Activate NADPH Oxidases
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 light chain
Alternative name(s):
Cytochrome b(558) alpha chain
Cytochrome b558 subunit alpha
Neutrophil cytochrome b 22 kDa polypeptide
Superoxide-generating NADPH oxidase light chain subunit
p22 phagocyte B-cytochrome
p22-phox
Short name:
p22phox
Gene namesi
Name:Cyba
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620573 Cyba

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei91 – 127By similarityAdd BLAST37

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449111 – 192Cytochrome b-245 light chainAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphothreonineBy similarity1
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei168PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1

Post-translational modificationi

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.By similarity
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.By similarity
Ubiquitinated at Lys-149 likely by RNF145.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62737
PRIDEiQ62737

PTM databases

iPTMnetiQ62737
PhosphoSitePlusiQ62737

Expressioni

Tissue specificityi

Expressed to a relatively high level in kidney, spleen, thymus and lung, and to a lower level in aorta, adrenals, and heart. Expression is not detected in liver or brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000013014
GenevisibleiQ62737 RN

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017564

Structurei

3D structure databases

SMRiQ62737
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the p22phox family.Curated

Phylogenomic databases

eggNOGiENOG410IXYN Eukaryota
ENOG4111MI6 LUCA
GeneTreeiENSGT00390000002290
HOGENOMiHOG000001585
HOVERGENiHBG051278
InParanoidiQ62737
KOiK08009
OMAiKIEWAMW
OrthoDBiEOG091G0NNC
PhylomeDBiQ62737

Family and domain databases

InterProiView protein in InterPro
IPR007732 Cyt_b558_asu
PANTHERiPTHR15168 PTHR15168, 1 hit
PfamiView protein in Pfam
PF05038 Cytochrom_B558a, 1 hit
PIRSFiPIRSF019635 Cytochr_b558a, 1 hit

Sequencei

Sequence statusi: Complete.

Q62737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVLIC
60 70 80 90 100
LLEYPRGKRK KGSTMERCGQ KYLTAVVKLF GPLTRNYYVR AVLHLLLSVP
110 120 130 140 150
AGFLLATILG TVCLAIASVI YLLAAIRGEQ WTPIEPKPKE RPQVGGTIKQ
160 170 180 190
PPTNPPPRPP AEVRKKPSEA EEEAASAGGP QVNPIPVTDE VV
Length:192
Mass (Da):20,750
Last modified:January 23, 2007 - v3
Checksum:iEDFCB82C2D217AEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18729 mRNA Translation: AAA85865.1
AJ295951 mRNA Translation: CAC09434.1
RefSeqiNP_077074.1, NM_024160.1
UniGeneiRn.5856

Genome annotation databases

EnsembliENSRNOT00000017564; ENSRNOP00000017564; ENSRNOG00000013014
GeneIDi79129
KEGGirno:79129
UCSCiRGD:620573 rat

Similar proteinsi

Entry informationi

Entry nameiCY24A_RAT
AccessioniPrimary (citable) accession number: Q62737
Secondary accession number(s): Q9ER27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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