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Protein

Cytochrome b-245 light chain

Gene

Cyba

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941Iron (heme axial ligand)Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: RGD
  • cellular response to angiotensin Source: RGD
  • cellular response to gamma radiation Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to L-glutamine Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to organic substance Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • cytochrome complex assembly Source: Ensembl
  • hydrogen peroxide biosynthetic process Source: BHF-UCL
  • inflammatory response Source: Ensembl
  • innate immune response Source: Ensembl
  • negative regulation of glomerular filtration by angiotensin Source: RGD
  • oxidation-reduction process Source: BHF-UCL
  • positive regulation of cell growth Source: RGD
  • positive regulation of defense response to bacterium Source: Ensembl
  • positive regulation of endothelial cell proliferation Source: RGD
  • positive regulation of interleukin-6 production Source: Ensembl
  • positive regulation of mucus secretion Source: Ensembl
  • positive regulation of NAD(P)H oxidase activity Source: RGD
  • positive regulation of phagocytosis Source: Ensembl
  • positive regulation of reactive oxygen species biosynthetic process Source: Ensembl
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of superoxide anion generation Source: RGD
  • positive regulation of toll-like receptor 2 signaling pathway Source: Ensembl
  • positive regulation of tumor necrosis factor production Source: Ensembl
  • regulation of blood pressure Source: RGD
  • regulation of release of sequestered calcium ion into cytosol Source: Ensembl
  • respiratory burst Source: Ensembl
  • response to activity Source: RGD
  • response to aldosterone Source: RGD
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to interleukin-1 Source: RGD
  • response to nutrient levels Source: RGD
  • smooth muscle hypertrophy Source: BHF-UCL
  • superoxide anion generation Source: BHF-UCL
  • superoxide metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-1222556. ROS, RNS production in response to bacteria.
R-RNO-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-RNO-3299685. Detoxification of Reactive Oxygen Species.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 light chain
Alternative name(s):
Cytochrome b(558) alpha chain
Cytochrome b558 subunit alpha
Neutrophil cytochrome b 22 kDa polypeptide
Superoxide-generating NADPH oxidase light chain subunit
p22 phagocyte B-cytochrome
p22-phox
Short name:
p22phox
Gene namesi
Name:Cyba
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620573. Cyba.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei91 – 12737By similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • dendrite Source: RGD
  • endosome Source: Ensembl
  • focal adhesion Source: RGD
  • Golgi apparatus Source: RGD
  • mitochondrion Source: Ensembl
  • NADPH oxidase complex Source: BHF-UCL
  • neuronal cell body Source: RGD
  • nucleus Source: RGD
  • stress fiber Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 192191Cytochrome b-245 light chainPRO_0000144911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471PhosphothreonineBy similarity
Modified residuei168 – 1681PhosphoserineCombined sources
Modified residuei176 – 1761PhosphoserineCombined sources

Post-translational modificationi

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.By similarity
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62737.
PRIDEiQ62737.

PTM databases

iPTMnetiQ62737.
PhosphoSiteiQ62737.

Expressioni

Tissue specificityi

Expressed to a relatively high level in kidney, spleen, thymus and lung, and to a lower level in aorta, adrenals, and heart. Expression is not detected in liver or brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000013014.
GenevisibleiQ62737. RN.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017564.

Family & Domainsi

Sequence similaritiesi

Belongs to the p22phox family.Curated

Phylogenomic databases

eggNOGiENOG410IXYN. Eukaryota.
ENOG4111MI6. LUCA.
GeneTreeiENSGT00390000002290.
HOGENOMiHOG000001585.
HOVERGENiHBG051278.
InParanoidiQ62737.
KOiK08009.
OMAiKIEWAMW.
OrthoDBiEOG091G0NNC.
PhylomeDBiQ62737.

Family and domain databases

InterProiIPR007732. Cyt_b558_asu.
[Graphical view]
PANTHERiPTHR15168. PTHR15168. 1 hit.
PfamiPF05038. Cytochrom_B558a. 1 hit.
[Graphical view]
PIRSFiPIRSF019635. Cytochr_b558a. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVLIC
60 70 80 90 100
LLEYPRGKRK KGSTMERCGQ KYLTAVVKLF GPLTRNYYVR AVLHLLLSVP
110 120 130 140 150
AGFLLATILG TVCLAIASVI YLLAAIRGEQ WTPIEPKPKE RPQVGGTIKQ
160 170 180 190
PPTNPPPRPP AEVRKKPSEA EEEAASAGGP QVNPIPVTDE VV
Length:192
Mass (Da):20,750
Last modified:January 23, 2007 - v3
Checksum:iEDFCB82C2D217AEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18729 mRNA. Translation: AAA85865.1.
AJ295951 mRNA. Translation: CAC09434.1.
RefSeqiNP_077074.1. NM_024160.1.
UniGeneiRn.5856.

Genome annotation databases

EnsembliENSRNOT00000017564; ENSRNOP00000017564; ENSRNOG00000013014.
GeneIDi79129.
KEGGirno:79129.
UCSCiRGD:620573. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18729 mRNA. Translation: AAA85865.1.
AJ295951 mRNA. Translation: CAC09434.1.
RefSeqiNP_077074.1. NM_024160.1.
UniGeneiRn.5856.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017564.

PTM databases

iPTMnetiQ62737.
PhosphoSiteiQ62737.

Proteomic databases

PaxDbiQ62737.
PRIDEiQ62737.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017564; ENSRNOP00000017564; ENSRNOG00000013014.
GeneIDi79129.
KEGGirno:79129.
UCSCiRGD:620573. rat.

Organism-specific databases

CTDi1535.
RGDi620573. Cyba.

Phylogenomic databases

eggNOGiENOG410IXYN. Eukaryota.
ENOG4111MI6. LUCA.
GeneTreeiENSGT00390000002290.
HOGENOMiHOG000001585.
HOVERGENiHBG051278.
InParanoidiQ62737.
KOiK08009.
OMAiKIEWAMW.
OrthoDBiEOG091G0NNC.
PhylomeDBiQ62737.

Enzyme and pathway databases

ReactomeiR-RNO-1222556. ROS, RNS production in response to bacteria.
R-RNO-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-RNO-3299685. Detoxification of Reactive Oxygen Species.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

PROiQ62737.

Gene expression databases

BgeeiENSRNOG00000013014.
GenevisibleiQ62737. RN.

Family and domain databases

InterProiIPR007732. Cyt_b558_asu.
[Graphical view]
PANTHERiPTHR15168. PTHR15168. 1 hit.
PfamiPF05038. Cytochrom_B558a. 1 hit.
[Graphical view]
PIRSFiPIRSF019635. Cytochr_b558a. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCY24A_RAT
AccessioniPrimary (citable) accession number: Q62737
Secondary accession number(s): Q9ER27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.