Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lamina-associated polypeptide 2, isoform beta

Gene

Tmpo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds directly to lamin B1 and chromosomes in a mitotic phosphorylation-regulated manner. May play an important role in nuclear envelope reassembly at the end of mitosis and/or anchoring of the nuclear lamina and interphase chromosomes to the nuclear envelope.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • lamin binding Source: RGD

GO - Biological processi

  • nuclear envelope reassembly Source: RGD
  • regulation of transcription, DNA-templated Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoform beta
Alternative name(s):
Thymopoietin isoform beta
Short name:
TP beta
Gene namesi
Name:Tmpo
Synonyms:Lap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3875. Tmpo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei410 – 43021Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini431 – 45222LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chromatin Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • nuclear inner membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Lamina-associated polypeptide 2, isoform betaPRO_0000206147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei67 – 671PhosphoserineCombined sources
Modified residuei74 – 741PhosphothreonineCombined sources
Modified residuei82 – 821PhosphoserineBy similarity
Modified residuei155 – 1551PhosphoserineCombined sources
Modified residuei158 – 1581PhosphoserineBy similarity
Modified residuei159 – 1591PhosphothreonineCombined sources
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei167 – 1671PhosphoserineCombined sources
Modified residuei176 – 1761PhosphoserineBy similarity
Modified residuei179 – 1791PhosphoserineBy similarity
Modified residuei206 – 2061N6-acetyllysineBy similarity
Modified residuei305 – 3051PhosphoserineBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei361 – 3611PhosphoserineBy similarity
Modified residuei388 – 3881N6-acetyllysineBy similarity

Post-translational modificationi

Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62733.
PRIDEiQ62733.

PTM databases

iPTMnetiQ62733.
PhosphoSiteiQ62733.

Expressioni

Gene expression databases

ExpressionAtlasiQ62733. baseline and differential.
GenevisibleiQ62733. RN.

Interactioni

Subunit structurei

Interacts with LMNB1, LMNB2, BANF1, AKAP8L, GMCL and chromosomes.By similarity

GO - Molecular functioni

  • lamin binding Source: RGD

Protein-protein interaction databases

BioGridi247398. 1 interaction.
MINTiMINT-4997319.
STRINGi10116.ENSRNOP00000012715.

Structurei

3D structure databases

ProteinModelPortaliQ62733.
SMRiQ62733. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 4844LEM-likePROSITE-ProRule annotationAdd
BLAST
Domaini108 – 15245LEMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 409409NucleoplasmicSequence analysisAdd
BLAST
Regioni49 – 10759LinkerAdd
BLAST
Regioni137 – 242106NAKAP95-binding NAdd
BLAST
Regioni298 – 37073Binds lamins BAdd
BLAST
Regioni299 – 37375NAKAP95-binding CAdd
BLAST

Domaini

Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the LEM family.Curated
Contains 1 LEM domain.PROSITE-ProRule annotation
Contains 1 LEM-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT7C. Eukaryota.
ENOG4111J0N. LUCA.
HOGENOMiHOG000113280.
HOVERGENiHBG000166.
InParanoidiQ62733.
PhylomeDBiQ62733.

Family and domain databases

Gene3Di1.10.720.40. 2 hits.
InterProiIPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
SM01261. Thymopoietin. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiPS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLAAGANSK GPPDFSSDEE REPTPVLGSG ASVGRGRGAV GRKATKKTDK
110 120 130 140 150
PRPEDKDDLD VTELSNEELL EQLVRYGVNP GPIVGTTRKL YEKKLLKLRE
160 170 180 190 200
QGAESRSSTP LPTVSSSAEN TRQNGSNDSD RYSDNDEDSK IELKLEKREP
210 220 230 240 250
LKGRAKTPVT LKQRRIEHNQ SYSEAGVTET EWTSGSSKGG PLQALTREST
260 270 280 290 300
RGSRRTPRRR VEPSQHFRVD GAVISESTPI AETIKASSND SLVANRLTGN
310 320 330 340 350
FKHASSILPI TEFSDITRRT PKKPLTRAEV GEKTEERRVE RDILKEMFPY
360 370 380 390 400
EASTPTGISA SCRRPIKGAA GRPLELSDFR MEESFSSKYV PKYVPLADVK
410 420 430 440 450
SEKTKKGRSV PMWIKMLLFA LVAGFLFLVY QAMETNQGNP FTNFLQDTKI

SN
Length:452
Mass (Da):50,278
Last modified:January 23, 2007 - v3
Checksum:iD56C9B51DB9D3DA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18314 mRNA. Translation: AAC52209.1.
PIRiS55255.
RefSeqiNP_037019.1. NM_012887.1.
UniGeneiRn.202955.

Genome annotation databases

EnsembliENSRNOT00000012715; ENSRNOP00000012715; ENSRNOG00000008797.
GeneIDi25359.
KEGGirno:25359.
UCSCiRGD:3875. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18314 mRNA. Translation: AAC52209.1.
PIRiS55255.
RefSeqiNP_037019.1. NM_012887.1.
UniGeneiRn.202955.

3D structure databases

ProteinModelPortaliQ62733.
SMRiQ62733. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247398. 1 interaction.
MINTiMINT-4997319.
STRINGi10116.ENSRNOP00000012715.

PTM databases

iPTMnetiQ62733.
PhosphoSiteiQ62733.

Proteomic databases

PaxDbiQ62733.
PRIDEiQ62733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012715; ENSRNOP00000012715; ENSRNOG00000008797.
GeneIDi25359.
KEGGirno:25359.
UCSCiRGD:3875. rat.

Organism-specific databases

CTDi7112.
RGDi3875. Tmpo.

Phylogenomic databases

eggNOGiENOG410IT7C. Eukaryota.
ENOG4111J0N. LUCA.
HOGENOMiHOG000113280.
HOVERGENiHBG000166.
InParanoidiQ62733.
PhylomeDBiQ62733.

Gene expression databases

ExpressionAtlasiQ62733. baseline and differential.
GenevisibleiQ62733. RN.

Family and domain databases

Gene3Di1.10.720.40. 2 hits.
InterProiIPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
SM01261. Thymopoietin. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiPS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope."
    Furukawa K., Pante N., Aebi U., Gerace L.
    EMBO J. 14:1626-1636(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 158-172; 270-284 AND 347-361.
    Tissue: Liver.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-67; THR-74; SER-155; THR-159 AND SER-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLAP2_RAT
AccessioniPrimary (citable) accession number: Q62733
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.