Reviewed, UniProtKB/Swiss-Prot Q62730 (DHB2_RAT)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 2
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 2
      Short name=17-beta-HSD 2

Gene names

Name:	Hsd17b2
Synonyms:	Edh17b2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Favors the oxidation of estradiol and testosterone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.
Subcellular location	Membrane; Single-pass type II membrane proteinPotential.
Tissue specificity	Highest expression in placenta.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Estradiol 17-beta-dehydrogenase 2		PRO_0000054572
Regions
	Transmembrane	4 – 24	21	Signal-anchor for type II membrane protein Potential
	Nucleotide binding	83 – 112	30	NAD By similarity
Sites
	Active site	233	1	Proton acceptor By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62730-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8E1D08DF345DE136	FASTA	381	41,967
	10 20 30 40 50 60 MNPFSSESAW LCLTATAVLG GMLLCKAWSS GQLRSQVVCL AGLWGGACLL SLSLLCSLFL 70 80 90 100 110 120 LSVSCFFLLY VSSSDQDLLP VDQKAVLVTG ADSGFGHALA KHLDKLGFTV FAGVLDKEGP 130 140 150 160 170 180 GAEELRKNCS ERLSVLQMDV TKPEQIKDVH SEVAEKIQDK GLWAVVNNAG VLHFPIDGEL 190 200 210 220 230 240 IPMTVYRKCM AVNFFGAVEV TKVFLPLLRK SKGRLVNVSS MGAMIPFQMV AAYASTKAAI 250 260 270 280 290 300 SMFSAVIRQE LAKWGVKVVT IHPGGFQTNI VGSQDSWDKM EKEILDHFSK EIQENYGQEY 310 320 330 340 350 360 VHTQKLALPV MREMSNPDIT PVLRDIQHAI CAKNPSSFYC SGRMTYLWIC FAAYSPISLL 370 380 DYILKNYFTP KLMPRALRTA S

References

[1]

"Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and characterization of tissue distribution and catalytic activity of rat type 1 and type 2 enzymes." Akinola L.A., Poutanen M., Vihko R. Endocrinology 137:1572-1579(1996) [PubMed: 8612487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Placenta.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91234 mRNA. Translation: CAA62617.1.
RefSeq	NP_077367.1.
UniGene	Rn.10515
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000013982. Rattus norvegicus. [Contig view]
GeneID	79243.
KEGG	rno:79243.
Organism-specific databases
RGD	621803. Hsd17b2.
Phylogenomic databases
HOVERGEN	Q62730.
Gene expression databases
ArrayExpress	Q62730.
GermOnline	ENSRNOG00000013982. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614690.

Entry information

Entry name

DHB2_RAT

Accession

Primary (citable) accession number: Q62730

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)