Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q62730 (DHB2_RAT)

Last modified November 25, 2008. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Estradiol 17-beta-dehydrogenase 2
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 2
      Short name=17-beta-HSD 2
Gene names
Name: Hsd17b2
Synonyms: Edh17b2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Favors the oxidation of estradiol and testosterone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH By similarity.

Catalytic activity

Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H.

Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.

Subcellular location

Membrane; Single-pass type II membrane proteinPotential.

Tissue specificity

Highest expression in placenta.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Estradiol 17-beta-dehydrogenase 2
PRO_0000054572

Regions

Transmembrane4 – 2421Signal-anchor for type II membrane protein Potential
Nucleotide binding83 – 11230NAD By similarity

Sites

Active site2331Proton acceptor By similarity
Binding site2201Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q62730-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8E1D08DF345DE136

FASTA38141,967
        10         20         30         40         50         60 
MNPFSSESAW LCLTATAVLG GMLLCKAWSS GQLRSQVVCL AGLWGGACLL SLSLLCSLFL 

        70         80         90        100        110        120 
LSVSCFFLLY VSSSDQDLLP VDQKAVLVTG ADSGFGHALA KHLDKLGFTV FAGVLDKEGP 

       130        140        150        160        170        180 
GAEELRKNCS ERLSVLQMDV TKPEQIKDVH SEVAEKIQDK GLWAVVNNAG VLHFPIDGEL 

       190        200        210        220        230        240 
IPMTVYRKCM AVNFFGAVEV TKVFLPLLRK SKGRLVNVSS MGAMIPFQMV AAYASTKAAI 

       250        260        270        280        290        300 
SMFSAVIRQE LAKWGVKVVT IHPGGFQTNI VGSQDSWDKM EKEILDHFSK EIQENYGQEY 

       310        320        330        340        350        360 
VHTQKLALPV MREMSNPDIT PVLRDIQHAI CAKNPSSFYC SGRMTYLWIC FAAYSPISLL 

       370        380 
DYILKNYFTP KLMPRALRTA S 

« Hide

References

[1]"Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and characterization of tissue distribution and catalytic activity of rat type 1 and type 2 enzymes."
Akinola L.A., Poutanen M., Vihko R.
Endocrinology 137:1572-1579(1996) [PubMed: 8612487] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Placenta.

Cross-references

Sequence databases

X91234 mRNA. Translation: CAA62617.1.
RefSeqNP_077367.1.
UniGeneRn.10515

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000013982. Rattus norvegicus. [Contig view]
GeneID79243.
KEGGrno:79243.

Organism-specific databases

RGD621803. Hsd17b2.

Phylogenomic databases

HOVERGENQ62730.

Gene expression databases

ArrayExpressQ62730.
GermOnlineENSRNOG00000013982. Rattus norvegicus.

Family and domain databases

InterProIPR002198. DHase_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR19410. ADH_short_C2. 1 hit.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio614690.

Entry information

Entry nameDHB2_RAT
AccessionPrimary (citable) accession number: Q62730
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents