Q62730 (DHB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Estradiol 17-beta-dehydrogenase 2
EC=1.1.1.62

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 2
Short name=17-beta-HSD 2
Testosterone 17-beta-dehydrogenase
EC=1.1.1.239

Gene names

Name:	Hsd17b2
Synonyms:	Edh17b2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Favors the oxidation of estradiol and testosterone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH By similarity.
Catalytic activity	17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H. Testosterone + NAD+ = androstenedione + NADH.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Highest expression in placenta.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen biosynthetic process Inferred from direct assay Ref.1. Source: RGD bone development Inferred from expression pattern PubMed 15012600. Source: RGD cellular response to metal ion Inferred from expression pattern PubMed 18602937. Source: RGD estrogen biosynthetic process Inferred from direct assay Ref.1. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular membrane-bounded organelle Inferred from direct assay PubMed 16940216. Source: RGD
Molecular_function	estradiol 17-beta-dehydrogenase activity Inferred from direct assay Ref.1. Source: RGD nucleotide binding Inferred from electronic annotation. Source: InterPro testosterone 17-beta-dehydrogenase (NAD+) activity Inferred from direct assay Ref.1. Source: RGD testosterone dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Estradiol 17-beta-dehydrogenase 2		PRO_0000054572
Regions
	Transmembrane	4 – 24	21	Helical; Signal-anchor for type II membrane protein; Potential
	Nucleotide binding	83 – 112	30	NAD By similarity
Sites
	Active site	233	1	Proton acceptor By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62730 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8E1D08DF345DE136	FASTA	381	41,967
	10 20 30 40 50 60 MNPFSSESAW LCLTATAVLG GMLLCKAWSS GQLRSQVVCL AGLWGGACLL SLSLLCSLFL 70 80 90 100 110 120 LSVSCFFLLY VSSSDQDLLP VDQKAVLVTG ADSGFGHALA KHLDKLGFTV FAGVLDKEGP 130 140 150 160 170 180 GAEELRKNCS ERLSVLQMDV TKPEQIKDVH SEVAEKIQDK GLWAVVNNAG VLHFPIDGEL 190 200 210 220 230 240 IPMTVYRKCM AVNFFGAVEV TKVFLPLLRK SKGRLVNVSS MGAMIPFQMV AAYASTKAAI 250 260 270 280 290 300 SMFSAVIRQE LAKWGVKVVT IHPGGFQTNI VGSQDSWDKM EKEILDHFSK EIQENYGQEY 310 320 330 340 350 360 VHTQKLALPV MREMSNPDIT PVLRDIQHAI CAKNPSSFYC SGRMTYLWIC FAAYSPISLL 370 380 DYILKNYFTP KLMPRALRTA S

References

[1]	"Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and characterization of tissue distribution and catalytic activity of rat type 1 and type 2 enzymes." Akinola L.A., Poutanen M., Vihko R. Endocrinology 137:1572-1579(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91234 mRNA. Translation: CAA62617.1.
IPI	IPI00208111.
RefSeq	NP_077367.1. NM_024391.1.
UniGene	Rn.10515.
3D structure databases
ProteinModelPortal	Q62730.
ModBase	Search...
Proteomic databases
PaxDb	Q62730.
PRIDE	Q62730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	79243.
KEGG	rno:79243.
UCSC	RGD:621803. rat.
Organism-specific databases
CTD	3294.
RGD	621803. Hsd17b2.
Phylogenomic databases
eggNOG	COG1028.
HOVERGEN	HBG005482.
InParanoid	Q62730.
KO	K13368.
OrthoDB	EOG4GB768.
Gene expression databases
ArrayExpress	Q62730.
Genevestigator	Q62730.
GermOnline	ENSRNOG00000013982. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1914268.
NextBio	614690.

Entry information

Entry name

DHB2_RAT

Accession

Primary (citable) accession number: Q62730

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program