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Q62730

- DHB2_RAT

UniProt

Q62730 - DHB2_RAT

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Protein

Estradiol 17-beta-dehydrogenase 2

Gene

Hsd17b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Favors the oxidation of estradiol and testosterone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity).By similarity

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Testosterone + NAD+ = androstenedione + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201SubstrateBy similarity
Active sitei233 – 2331Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 11230NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. estradiol 17-beta-dehydrogenase activity Source: RGD
  2. testosterone dehydrogenase (NAD+) activity Source: RGD

GO - Biological processi

  1. androgen biosynthetic process Source: RGD
  2. bone development Source: RGD
  3. cellular response to metal ion Source: RGD
  4. estrogen biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17-beta-dehydrogenase 2 (EC:1.1.1.62)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 2
Short name:
17-beta-HSD 2
Testosterone 17-beta-dehydrogenase (EC:1.1.1.239)
Gene namesi
Name:Hsd17b2
Synonyms:Edh17b2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621803. Hsd17b2.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Estradiol 17-beta-dehydrogenase 2PRO_0000054572Add
BLAST

Proteomic databases

PaxDbiQ62730.
PRIDEiQ62730.

Expressioni

Tissue specificityi

Highest expression in placenta.

Gene expression databases

GenevestigatoriQ62730.

Interactioni

Protein-protein interaction databases

MINTiMINT-4566728.

Structurei

3D structure databases

ProteinModelPortaliQ62730.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG005482.
InParanoidiQ62730.
KOiK13368.
PhylomeDBiQ62730.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62730-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPFSSESAW LCLTATAVLG GMLLCKAWSS GQLRSQVVCL AGLWGGACLL
60 70 80 90 100
SLSLLCSLFL LSVSCFFLLY VSSSDQDLLP VDQKAVLVTG ADSGFGHALA
110 120 130 140 150
KHLDKLGFTV FAGVLDKEGP GAEELRKNCS ERLSVLQMDV TKPEQIKDVH
160 170 180 190 200
SEVAEKIQDK GLWAVVNNAG VLHFPIDGEL IPMTVYRKCM AVNFFGAVEV
210 220 230 240 250
TKVFLPLLRK SKGRLVNVSS MGAMIPFQMV AAYASTKAAI SMFSAVIRQE
260 270 280 290 300
LAKWGVKVVT IHPGGFQTNI VGSQDSWDKM EKEILDHFSK EIQENYGQEY
310 320 330 340 350
VHTQKLALPV MREMSNPDIT PVLRDIQHAI CAKNPSSFYC SGRMTYLWIC
360 370 380
FAAYSPISLL DYILKNYFTP KLMPRALRTA S
Length:381
Mass (Da):41,967
Last modified:November 1, 1996 - v1
Checksum:i8E1D08DF345DE136
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91234 mRNA. Translation: CAA62617.1.
RefSeqiNP_077367.1. NM_024391.1.
UniGeneiRn.10515.

Genome annotation databases

GeneIDi79243.
KEGGirno:79243.
UCSCiRGD:621803. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91234 mRNA. Translation: CAA62617.1 .
RefSeqi NP_077367.1. NM_024391.1.
UniGenei Rn.10515.

3D structure databases

ProteinModelPortali Q62730.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4566728.

Chemistry

ChEMBLi CHEMBL1914268.

Proteomic databases

PaxDbi Q62730.
PRIDEi Q62730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 79243.
KEGGi rno:79243.
UCSCi RGD:621803. rat.

Organism-specific databases

CTDi 3294.
RGDi 621803. Hsd17b2.

Phylogenomic databases

eggNOGi COG1028.
HOVERGENi HBG005482.
InParanoidi Q62730.
KOi K13368.
PhylomeDBi Q62730.

Miscellaneous databases

NextBioi 614690.
PROi Q62730.

Gene expression databases

Genevestigatori Q62730.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and characterization of tissue distribution and catalytic activity of rat type 1 and type 2 enzymes."
    Akinola L.A., Poutanen M., Vihko R.
    Endocrinology 137:1572-1579(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Placenta.

Entry informationi

Entry nameiDHB2_RAT
AccessioniPrimary (citable) accession number: Q62730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3