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Protein

Serine/threonine-protein kinase ICK

Gene

Ick

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for ciliogenesis, particularly in neuronal and retinal progenitor cells (By similarity). Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length (By similarity). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a role in cardiac development (PubMed:8570168).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPBy similarityPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cilium assembly Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. intraciliary transport Source: UniProtKB
  4. multicellular organismal development Source: UniProtKB-KW
  5. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ICK (EC:2.7.11.22)
Alternative name(s):
Intestinal cell kinase
MAK-related kinase
Short name:
MRK
Gene namesi
Name:Ick
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi71050. Ick.

Subcellular locationi

Cytoplasm 1 Publication. Cell projectioncilium By similarity. Nucleus By similarity. Cytoplasmcytoskeletoncilium basal body By similarity
Note: Also found at the ciliary tip (By similarity).By similarity1 Publication

GO - Cellular componenti

  1. ciliary basal body Source: UniProtKB
  2. ciliary tip Source: UniProtKB
  3. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629Serine/threonine-protein kinase ICKPRO_0000086009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphothreonineBy similarity
Modified residuei159 – 1591PhosphotyrosineBy similarity

Post-translational modificationi

Autophosphorylated on serine and threonine residues. May play a role in enzyme activation.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62726.
PRIDEiQ62726.

PTM databases

PhosphoSiteiQ62726.

Expressioni

Tissue specificityi

Expressed in embryonic heart from day 11. Highly expressed in the uterus and at lower levels in brain, heart, lung, kidney, skeletal muscle, ovary and liver in adult tissues.1 Publication

Gene expression databases

GenevestigatoriQ62726.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012257.

Structurei

3D structure databases

ProteinModelPortaliQ62726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 284281Protein kinaseCuratedPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ62726.
KOiK08828.
PhylomeDBiQ62726.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62726-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRYTTIKQL GDGTYGSVLL GRSIESGELI AIKKMKRKFY SWEECMNLRE
60 70 80 90 100
VKSLKKLNHA NIVKLKEVIR ENDHLYFIFE YMKENLYQLI KERNKLFPES
110 120 130 140 150
AIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREI
160 170 180 190 200
RSRPPYTDYV STRWYRAPEV LLRSTNYSSP IDVWAVGCIM AEVYTLRPLF
210 220 230 240 250
PGASEIDTIF KICQVLGTPK KTDWPEGYQL SSAMNFIWPQ CIPNNLKTLI
260 270 280 290 300
PNASSEAIQL LRDLLQWDPK KRPTASQALR YPYFQIGHPL GISTQDSGKP
310 320 330 340 350
QKDVQDKTGP PPYVKPAPPA QAPTKAHTLI SSRPNQASQP HQHFVYPYKG
360 370 380 390 400
EASRTEQLSH VQEGQPNPPF FPSLHNKNLP PKILAGLEQK SGDMKPKSRR
410 420 430 440 450
RWGLISRSTK GSDDWADLAD LDFSSSLTRI DVKNKKRQSD DPLCRFESVL
460 470 480 490 500
DLKPSEPVGT GTSVSTQASS QRRDTPTLQS TAKQHYLKHS RYLPGINIRN
510 520 530 540 550
GVLPNPGKDF LPSSSWSSSG LSGKSSGTVS VVSKITSVGS GSTSSTGLTG
560 570 580 590 600
SYIPSFLKKE VGSVMQRVQL APLAAPSPGY SSLKAVRPHP GRPFFHTQPR
610 620
STPGLIPRPP AVQPVHGRID WSSKYPSRR
Length:629
Mass (Da):70,569
Last modified:November 1, 1996 - v1
Checksum:iAA4143D73F891E8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26178 mRNA. Translation: BAA05166.1.
RefSeqiNP_620241.1. NM_138886.1.
UniGeneiRn.3750.

Genome annotation databases

GeneIDi84411.
KEGGirno:84411.
UCSCiRGD:71050. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26178 mRNA. Translation: BAA05166.1.
RefSeqiNP_620241.1. NM_138886.1.
UniGeneiRn.3750.

3D structure databases

ProteinModelPortaliQ62726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012257.

PTM databases

PhosphoSiteiQ62726.

Proteomic databases

PaxDbiQ62726.
PRIDEiQ62726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84411.
KEGGirno:84411.
UCSCiRGD:71050. rat.

Organism-specific databases

CTDi22858.
RGDi71050. Ick.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ62726.
KOiK08828.
PhylomeDBiQ62726.

Miscellaneous databases

NextBioi616888.
PROiQ62726.

Gene expression databases

GenevestigatoriQ62726.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a novel serine/threonine kinase, MRK, possibly involved in cardiac development."
    Abe S., Yagi T., Ishiyama S., Hiroe M., Marumo F., Ikawa Y.
    Oncogene 11:2187-2195(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
    Tissue: HeartImported.

Entry informationi

Entry nameiICK_RAT
AccessioniPrimary (citable) accession number: Q62726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.