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Protein

DNA replication licensing factor MCM6

Gene

Mcm6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 898ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM6 (EC:3.6.4.12)
Alternative name(s):
Intestinal DNA replication protein
Gene namesi
Name:Mcm6
Synonyms:Mcmd6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61967. Mcm6.

Subcellular locationi

  • Nucleus By similarity

  • Note: Binds to chromatin during G1 and detach from it during S phase.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 507›507DNA replication licensing factor MCM6PRO_0000194115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei329 – 3291N6-acetyllysineBy similarity
Modified residuei375 – 3751PhosphoserineBy similarity
Modified residuei390 – 3901PhosphoserineBy similarity
Modified residuei448 – 4481PhosphoserineCombined sources
Modified residuei477 – 4771PhosphothreonineBy similarity

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62724.
PeptideAtlasiQ62724.
PRIDEiQ62724.

PTM databases

iPTMnetiQ62724.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). May interact with MCM10. Interacts with TIPIN. Interacts with MCMBP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004969.

Structurei

3D structure databases

ProteinModelPortaliQ62724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 239208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi214 – 2174Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
HOVERGENiHBG006334.
InParanoidiQ62724.
PhylomeDBiQ62724.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q62724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LRDEEQTAES IKNQMTVKEW EKVFEMSQDQ NLYHNLCTSL FPTIHGNDEV
60 70 80 90 100
KRGVLLMLFG GVPKTTGEGT SLRGDINVCI VGDPSTAKSQ FLKHVDEFSP
110 120 130 140 150
RAVYTSGKAS SASGLTAAVV RDEESHEFVI EAGALMLADN GVCCIDEFDK
160 170 180 190 200
MDMRDQVAIH EAMEQQTISI TKAGVKATLN ARTSILAAAN PVSGHYDRSK
210 220 230 240 250
SLKQNINLSA PIMSRFDLFF ILVDECNEVT DYAIARRIVD LHSRIEESID
260 270 280 290 300
RVYSLDDIRR YLLFARQFKP KISKESEDFI VEQYKRLRQR DGSGITKSSW
310 320 330 340 350
RITVRQLESM IRLSESMARM HCCDEVQPKH VKEAFRLLNK SIIRVETPDV
360 370 380 390 400
NLDQEEEIQM ETDEGPGGIN GHADSPAPVN GFNGSGEDAS QETVPKPSLR
410 420 430 440 450
LAFAEYCRIS NLIVLHLRKM EEEEDESALK RSELVNWYLK EIESEIDSEE
460 470 480 490 500
ELINKKRIIE KVVHRLTHYD HVLIELTQAG LKGSSEGSES YEEDPYLVVN

PNYLLED
Length:507
Mass (Da):57,370
Last modified:December 15, 1998 - v2
Checksum:i03CE569FFFCDC654
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17565 mRNA. Translation: AAC18424.1.
PIRiT10753.
UniGeneiRn.33226.

Genome annotation databases

UCSCiRGD:61967. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17565 mRNA. Translation: AAC18424.1.
PIRiT10753.
UniGeneiRn.33226.

3D structure databases

ProteinModelPortaliQ62724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004969.

PTM databases

iPTMnetiQ62724.

Proteomic databases

PaxDbiQ62724.
PeptideAtlasiQ62724.
PRIDEiQ62724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:61967. rat.

Organism-specific databases

RGDi61967. Mcm6.

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
HOVERGENiHBG006334.
InParanoidiQ62724.
PhylomeDBiQ62724.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM6_RAT
AccessioniPrimary (citable) accession number: Q62724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: July 6, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.