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Protein

Calcium-dependent secretion activator 1

Gene

Cadps

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates catecholamine loading of DCVs. May specifically mediate the Ca2+-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles by acting as a PtdIns(4,5)P2-binding protein that acts at prefusion step following ATP-dependent priming and participates in DCVs-membrane fusion. However, it may also participate in small clear synaptic vesicles (SVs) exocytosis and it is unclear whether its function is related to Ca2+ triggering.6 Publications

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: WormBase

GO - Biological processi

  • dense core granule exocytosis Source: InterPro
  • positive regulation of calcium ion-dependent exocytosis Source: RGD
  • positive regulation of exocytosis Source: ParkinsonsUK-UCL
  • protein transport Source: UniProtKB-KW
  • regulated exocytosis Source: RGD
  • synaptic vesicle exocytosis Source: InterPro

Keywordsi

Biological processExocytosis, Protein transport, Transport
LigandCalcium, Lipid-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent secretion activator 1
Alternative name(s):
Calcium-dependent activator protein for secretion 1
Short name:
CAPS-1
Short name:
rCAPS
Gene namesi
Name:Cadps
Synonyms:Caps, Caps1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi708573 Cadps

Subcellular locationi

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi476G → E: Loss of function. 1 Publication1
Mutagenesisi531K → E: No effect. 1 Publication1
Mutagenesisi537 – 540WKKR → SQKE: Affects PtdIns(4,5)P2-binding, plasma membrane-association but not binding to DCVs. 1 Publication4
Mutagenesisi558 – 561REKK → DEEE: Affects PtdIns(4,5)P2-binding, plasma membrane-association but not binding to DCVs. 1 Publication4
Mutagenesisi561K → E: Affects PtdIns(4,5)P2-binding, plasma membrane-association but not binding to DCVs. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538661 – 1289Calcium-dependent secretion activator 1Add BLAST1289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei89PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1
Modified residuei259PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62717
PRIDEiQ62717

PTM databases

iPTMnetiQ62717
PhosphoSitePlusiQ62717

Expressioni

Tissue specificityi

Specifically expressed in neural and endocrine secretory tissues. Expressed in brain, pancreas, hypothalamus, pituitary and adrenal but not in heart, placenta, lung, liver, skeletal muscle or kidney. In brain, it is absent from the cell body layers of the hippocampus and dentate gyrus but abundant in the synaptic neuropil, except for the stratum lucidum and stratum lacunosum moleculare where it is not detected. In the cerebellum, it is mainly detected in the glomeruli of the granule cell layer where it colocalized with synaptophysin. In the olfactory bulb, it is detected mainly in the glomeruli. In the adult adrenal gland, where it is restricted to the medulla (at protein level).3 Publications

Interactioni

Subunit structurei

Interacts with the dopamine receptor DRD2 (By similarity). Interacts with RASL10B (By similarity). Homodimer.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx1aP328513EBI-15804323,EBI-539720

Protein-protein interaction databases

BioGridi247806, 1 interactor
DIPiDIP-58640N
IntActiQ62717, 2 interactors
STRINGi10116.ENSRNOP00000012153

Structurei

3D structure databases

ProteinModelPortaliQ62717
SMRiQ62717
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini398 – 479C2Add BLAST82
Domaini519 – 622PHPROSITE-ProRule annotationAdd BLAST104
Domaini916 – 1047MHD1PROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni788 – 1065Interaction with DRD2By similarityAdd BLAST278
Regioni1113 – 1289Mediates targeting and association with DCVsAdd BLAST177

Domaini

The PH domain is essential for regulated exocytosis and binds phospholipids and plasma membrane. It however does not mediate binding to DCVs.1 Publication

Phylogenomic databases

eggNOGiKOG3543 Eukaryota
ENOG410Y0FD LUCA
HOGENOMiHOG000263607
HOVERGENiHBG080678
InParanoidiQ62717
KOiK19933

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR033227 CAPS
IPR010439 CAPS_dom
IPR014770 Munc13_1
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR12166 PTHR12166, 2 hits
PfamiView protein in Pfam
PF06292 DUF1041, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM01145 DUF1041, 1 hit
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS51258 MHD1, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q62717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDPSSSEEE SDEILEEESG KEVLGSAASG ARLSPSRTNE GSAGSAGMGG
60 70 80 90 100
SGAGAGVGAG GGGGSGASSG GGAGGLQPSS RAGGGRPSSP SPSVVSEKEK
110 120 130 140 150
EELERLQKEE EERKKRLQLY VFVMRCIAYP FNAKQPTDMA RRPRKISKQQ
160 170 180 190 200
LQTVKDRFQA FLNGETQIVA DEAFMNAVQS YYEVFLKSDR VARMVQSGGC
210 220 230 240 250
SANDSREVFK KHIEKRVRSL PEIDGLSKET VLSSWMAKFD AIYRGEEDPR
260 270 280 290 300
KQQARMTASA ASELILSKEQ LYEMFQNILG IKKFEHQLLY NACQLDNPDE
310 320 330 340 350
QAAQIRRELD GRLQMADQIA RERKFPKFVS KEMENMYIEE LKSSVNLLMA
360 370 380 390 400
NLESMPVSKG GEFKLQKLKR SHNASIIDMG EESENQLSKS DVLLSFSLEV
410 420 430 440 450
VIMEVQGLKS LAPNRIVYCT MEVEGGEKLQ TDQAEASKPT WGTQGDFSTT
460 470 480 490 500
HALPAVKVKL FTESTGVLAL EDKELGRVIL HPTPNSPKQS EWHKMTVSKN
510 520 530 540 550
CPDQDLKIKL AVRMDKPQNM KHSGYLWTIG KNVWKRWKKR FFVLVQVSQY
560 570 580 590 600
TFAMCSYREK KAEPQELLQL DGYTVDYTDP QPGLEGGRAF FNAVKEGDTV
610 620 630 640 650
IFASDDEQDR ILWVQAMYRA TGQSHKPVPP TQVQKLKPRA ETCLSMDAPI
660 670 680 690 700
SQFYADRAQK HGMDEFISSN PCNFDHASLF EMVQRLTLDH RLNDSYSCLG
710 720 730 740 750
WFSPGQVFVL DEYCARNGVR GCHRHLCYLR DLLERAENGA MIDPTLLHYS
760 770 780 790 800
FAFCASHVHG NRPDGIGTVT VEEKERFEEI KERLRVLLEN QITHFRYCFP
810 820 830 840 850
FGRPEGALKA TLSLLERVLM KDIVTPVPQE EVKTVIRKCL EQAALVNYSR
860 870 880 890 900
LSEYAKIEEN VGRLITPAKK LEDTIRLAEL VIEVLQQNEE HHAEAFAWWS
910 920 930 940 950
DLMVEHAETF LSLFAVDMDA ALEVQPPDTW DSFPLFQLLN DFLRTDYNLC
960 970 980 990 1000
NGKFHKHLQD LFAPLVVRYV DLMESSIAQS IHRGFERESW EPVNNGSGTS
1010 1020 1030 1040 1050
EDLFWKLDAL QTFIRDLHWP EEEFGKHLEQ RLKLMASDMI ESCVKRTRIA
1060 1070 1080 1090 1100
FEVKLQKTSR STDFRVPQSI CTMFNVMVDA KAQSTKLCSM EMGQEHQYHS
1110 1120 1130 1140 1150
KIDELIEETV KEMITLLVAK FVTILEGVLA KLSRYDEGTL FSSFLSFTVK
1160 1170 1180 1190 1200
AASKYVDVPK PGMDVADAYV TFVRHSQDVL RDKVNEEMYI ERLFDQWYNS
1210 1220 1230 1240 1250
SMNVICTWLT DRMDLQLHIY QLKTLIRMVK KTYRDFRLQG VLDSTLNSKT
1260 1270 1280
YETIRNRLTV EERTASVSEG GGLQGISMKD SDEEDEEDD
Length:1,289
Mass (Da):146,266
Last modified:November 1, 1996 - v1
Checksum:i2ADAEA35FB20BCDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16802 mRNA Translation: AAB88635.1
PIRiI84505
RefSeqiNP_037351.1, NM_013219.1
UniGeneiRn.88197

Genome annotation databases

GeneIDi26989
KEGGirno:26989

Entry informationi

Entry nameiCAPS1_RAT
AccessioniPrimary (citable) accession number: Q62717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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