Q62703 (RCN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulocalbin-2

Alternative name(s):
Calcium-binding protein ERC-55
Taipoxin-associated calcium-binding protein 49
Short name=TCBP-49

Gene names

Name:	Rcn2
Synonyms:	Erc55

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known. Binds calcium.
Subunit structure	Binds the snake venom phospholipase complex taipoxin.
Subcellular location	Endoplasmic reticulum lumen.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the CREC family. Contains 6 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.1
	Chain	26 – 320	295	Reticulocalbin-2		PRO_0000004150
Regions
	Domain	64 – 99	36	EF-hand 1
	Domain	100 – 135	36	EF-hand 2
	Domain	150 – 185	36	EF-hand 3
	Domain	189 – 224	36	EF-hand 4
	Domain	230 – 265	36	EF-hand 5
	Domain	266 – 301	36	EF-hand 6
	Calcium binding	77 – 88	12	1 Potential
	Calcium binding	113 – 124	12	2 Potential
	Calcium binding	165 – 176	12	3; possibly ancestral Potential
	Calcium binding	202 – 213	12	4 Potential
	Calcium binding	243 – 254	12	5 Potential
	Calcium binding	279 – 290	12	6 Potential
	Motif	317 – 320	4	Prevents secretion from ER Potential
Experimental info
	Sequence conflict	17 – 18	2	Missing in AAA80197. Ref.1
	Sequence conflict	46	1	T → A in AAA80197. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q62703 [UniParc]. Last modified May 24, 2005. Version 2. Checksum: 92CCA62B17E2D1BB	FASTA	320	37,433
	10 20 30 40 50 60 MRLGPRPAVL GLLLLLLLYA AVAGASKAEE LHYPQGEHRA DYDRETLLGV QEDVDEYVKL 70 80 90 100 110 120 GHEEQQRRLQ SIIKKIDSDS DGFLTENELS QWIQMSFKHY AMQEAKQQFV EYDKNSDGTV 130 140 150 160 170 180 TWDEYNVQMY DRVIDFDENT ALDDTEEESF RQLHLKDKKR FEKANQDSGP GLNLEEFIAF 190 200 210 220 230 240 EHPEEVDYMT EFVIQEALEE HDKNGDGFVS LEEFLGDYRR DPTANEDPEW ILVEKDRFVN 250 260 270 280 290 300 DYDKDSDGRL DPQELLSWVV PNNQGIAQEE ALHLIDEMDL NSDKKLSEEE ILENQDLFLT 310 320 SEATDYGRQL HDDYFYHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel reticular calcium binding protein is purified on taipoxin columns." Dodds D., Schlimgen A.K., Lu S.Y., Perin M.S. J. Neurochem. 64:2339-2344(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-38 AND 130-154. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 133-151, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15734 mRNA. Translation: AAA80197.1. BC061962 mRNA. Translation: AAH61962.1.
IPI	IPI00326759.
PIR	I56519.
RefSeq	NP_058828.2. NM_017132.2.
UniGene	Rn.6133.
3D structure databases
ProteinModelPortal	Q62703.
ModBase	Search...
PTM databases
PhosphoSite	Q62703.
Proteomic databases
PaxDb	Q62703.
PRIDE	Q62703.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021817; ENSRNOP00000021817; ENSRNOG00000015780.
GeneID	29218.
KEGG	rno:29218.
UCSC	RGD:3548. rat.
Organism-specific databases
CTD	5955.
RGD	3548. Rcn2.
Phylogenomic databases
eggNOG	NOG124414.
GeneTree	ENSGT00550000074546.
HOGENOM	HOG000230934.
HOVERGEN	HBG002834.
InParanoid	Q62703.
OrthoDB	EOG4DFPP6.
Gene expression databases
Genevestigator	Q62703.
GermOnline	ENSRNOG00000015780. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 3 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13202. EF_hand_3. 1 hit. PF13499. EF_hand_5. 2 hits. [Graphical view]
SMART	SM00054. EFh. 5 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 5 hits. PS50222. EF_HAND_2. 5 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608405.

Entry information

Entry name

RCN2_RAT

Accession

Primary (citable) accession number: Q62703
Secondary accession number(s): Q6P6X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program