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Protein

Disks large homolog 1

Gene

Dlg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation.By similarity4 Publications

GO - Molecular functioni

  • guanylate kinase activity Source: GO_Central
  • ionotropic glutamate receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • phosphatase binding Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BRENDAi2.7.4.8. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large homolog 1
Alternative name(s):
Synapse-associated protein 97
Short name:
SAP-97
Short name:
SAP97
Gene namesi
Name:Dlg1
Synonyms:Dlgh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2505. Dlg1.

Subcellular locationi

GO - Cellular componenti

  • basal plasma membrane Source: RGD
  • basolateral plasma membrane Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cytosol Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • microvillus Source: MGI
  • neuron projection Source: RGD
  • paranode region of axon Source: MGI
  • plasma membrane Source: RGD
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: RGD
  • presynaptic membrane Source: RGD
  • T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39S → A: No enrichment in postsynaptic density upon CaMK2 activation. 1 Publication1
Mutagenesisi39S → D: Localizes at the postsynaptic density. 1 Publication1
Mutagenesisi232S → A: No effect on association with GRIN2B. 1 Publication1
Mutagenesisi232S → D: Partial loss of association with GRIN2B. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945501 – 911Disks large homolog 1Add BLAST911

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39Phosphoserine; by CaMK21 Publication1
Modified residuei122PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei232Phosphoserine; by CaMK21 Publication1
Modified residuei398PhosphotyrosineBy similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei572PhosphoserineBy similarity1
Modified residuei574PhosphoserineBy similarity1
Modified residuei578PhosphoserineBy similarity1
Modified residuei597PhosphoserineBy similarity1
Modified residuei618PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1
Modified residuei687PhosphoserineBy similarity1
Modified residuei841PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by MAPK12 (By similarity). Phosphorylation of Ser-39 modulates transport to the plasma membrane and phosphorylation of Ser-232 regulates association with GRIN2A.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62696.
PRIDEiQ62696.

PTM databases

iPTMnetiQ62696.
PhosphoSitePlusiQ62696.
SwissPalmiQ62696.

Expressioni

Tissue specificityi

Widely expressed. Strongly expressed in epithelial cells, in the small intestine it is only detected in the vili. Expressed in brain, heart (at protein level), muscle, lung and liver. In the brain it was detected in olfactory bulbs, cerebral cortex, hippocampus, and spinal cord (at protein level).2 Publications

Inductioni

By BDNF.1 Publication

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts (via guanylate kinase-like domain) with DLGAP1, DLGAP2, DLGAP3, DLGAP4 and MAP1A (PubMed:9115257, PubMed:9786987). Interacts (via guanylate kinase-like domain) with KIF13B (By similarity). May interact with HTR2A (By similarity). Interacts (via PDZ domains) with GRIA1 (PubMed:9677374). Interacts (via PDZ domains) with GRIN2A (PubMed:12933808). Interacts (via PDZ domains) with KCND2 and KCND3 (PubMed:19213956). Interacts (via PDZ domains) with KCNA1, KCNA2, KCNA3 and KCNA4 (By similarity). Interacts (via PDZ domains) with ADGRA3 (By similarity). Interacts with KCNF1 (By similarity). Interacts with CAMK2 (PubMed:19213956). Interacts with cytoskeleton-associated protein EPB41 (By similarity). Interacts with cytoskeleton-associated protein EZR (By similarity). Found in a complex with KCNA5 and CAV3 (By similarity). Found in a complex with APC and CTNNB1 (By similarity). Interacts with CDH1 through binding to PIK3R1 (By similarity). Forms multiprotein complexes with CASK, LIN7A, LIN7B, LIN7C, APBA1, and KCNJ12 (PubMed:11865057, PubMed:14960569). Interacts with TOPK (By similarity). Forms a tripartite complex composed of DLG1, MPP7 and LIN7 (LIN7A or LIN7C) (By similarity). May interact with TJAP1 (By similarity). Interacts with PTEN (PubMed:15951562). Interacts with FRMPD4 (via C-terminus) (PubMed:19118189). Interacts with LRFN1 and LRFN2 (PubMed:16495444, PubMed:16630835). Interacts with LRFN4 and SFPQ (By similarity). Interacts (via PDZ domains) with ADGRA2 (via PDZ-binding motif) (By similarity).By similarityCurated11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BTRCQ9Y2973EBI-389325,EBI-307461From a different organism.
DgkiQ810C53EBI-389325,EBI-8523614
DgkzO085604EBI-389325,EBI-8570505
Dlg4P310162EBI-389325,EBI-375655
DLGAP1O144902EBI-389325,EBI-1753207From a different organism.
E4P890794EBI-389325,EBI-7401124From a different organism.
Gpsm2Q8VDU07EBI-389325,EBI-7575403From a different organism.
Lrfn2Q460M52EBI-389325,EBI-877185
Map1aP349262EBI-389325,EBI-631571
PTENP604842EBI-389325,EBI-696162From a different organism.
Scn7aB1AYL13EBI-389325,EBI-8068354From a different organism.

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • phosphatase binding Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi247292. 16 interactors.
DIPiDIP-31527N.
IntActiQ62696. 27 interactors.
MINTiMINT-93379.
STRINGi10116.ENSRNOP00000055672.

Structurei

Secondary structure

1911
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 20Combined sources16
Beta strandi23 – 26Combined sources4
Helixi28 – 42Combined sources15
Helixi44 – 52Combined sources9
Beta strandi55 – 61Combined sources7
Beta strandi225 – 227Combined sources3
Beta strandi235 – 239Combined sources5
Beta strandi242 – 246Combined sources5
Beta strandi254 – 258Combined sources5
Beta strandi260 – 262Combined sources3
Helixi263 – 267Combined sources5
Beta strandi277 – 279Combined sources3
Helixi289 – 297Combined sources9
Beta strandi301 – 306Combined sources6
Beta strandi316 – 322Combined sources7
Beta strandi327 – 334Combined sources8
Beta strandi347 – 352Combined sources6
Helixi357 – 361Combined sources5
Beta strandi369 – 373Combined sources5
Helixi383 – 391Combined sources9
Beta strandi395 – 402Combined sources8
Beta strandi405 – 407Combined sources3
Beta strandi464 – 469Combined sources6
Beta strandi476 – 481Combined sources6
Beta strandi483 – 486Combined sources4
Beta strandi488 – 493Combined sources6
Helixi498 – 502Combined sources5
Helixi524 – 532Combined sources9
Beta strandi536 – 542Combined sources7
Beta strandi583 – 587Combined sources5
Turni593 – 595Combined sources3
Beta strandi611 – 616Combined sources6
Beta strandi619 – 628Combined sources10
Beta strandi638 – 641Combined sources4
Helixi643 – 651Combined sources9
Beta strandi709 – 717Combined sources9
Beta strandi724 – 728Combined sources5
Helixi731 – 741Combined sources11
Turni743 – 745Combined sources3
Turni763 – 765Combined sources3
Helixi773 – 781Combined sources9
Beta strandi785 – 791Combined sources7
Beta strandi794 – 799Combined sources6
Helixi800 – 807Combined sources8
Turni808 – 810Combined sources3
Beta strandi812 – 815Combined sources4
Helixi820 – 827Combined sources8
Beta strandi833 – 837Combined sources5
Helixi842 – 848Combined sources7
Helixi854 – 871Combined sources18
Helixi872 – 874Combined sources3
Beta strandi876 – 879Combined sources4
Helixi884 – 898Combined sources15
Beta strandi900 – 906Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RSONMR-A/C4-63[»]
1ZOKNMR-A221-313[»]
2AWUX-ray2.44A/B314-409[»]
2AWWX-ray2.21A/B314-409[»]
2AWXX-ray1.80A/B314-409[»]
2G2LX-ray2.35A/B314-409[»]
2I0IX-ray2.80A/B/C459-543[»]
2I0LX-ray2.31A/B318-401[»]
3UATX-ray2.70A578-911[»]
ProteinModelPortaliQ62696.
SMRiQ62696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62696.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 64L27PROSITE-ProRule annotationAdd BLAST61
Domaini224 – 310PDZ 1PROSITE-ProRule annotationAdd BLAST87
Domaini318 – 404PDZ 2PROSITE-ProRule annotationAdd BLAST87
Domaini465 – 545PDZ 3PROSITE-ProRule annotationAdd BLAST81
Domaini580 – 650SH3PROSITE-ProRule annotationAdd BLAST71
Domaini721 – 896Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST176

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni162 – 212Interaction with SH3 domainsBy similarityAdd BLAST51

Domaini

The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.By similarity
The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization (By similarity).By similarity

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 1 L27 domain.PROSITE-ProRule annotation
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0708. Eukaryota.
COG0194. LUCA.
HOGENOMiHOG000232102.
HOVERGENiHBG107814.
InParanoidiQ62696.
KOiK12076.
PhylomeDBiQ62696.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR016313. DLG1.
IPR019590. DLG1_PEST_dom.
IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR015143. L27_1.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF09058. L27_1. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PIRSFiPIRSF001741. MAGUK_DLGH. 1 hit.
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 1 hit.
SM01277. MAGUK_N_PEST. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 1 hit.
SSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVRKQDTQR ALHLLEEYRS KLSQTEDRQL RSSIERVISI FQSNLFQALI
60 70 80 90 100
DIQEFYEVTL LDNPKCVDHS KQCEPVQPGN PWESGSLSSA AVTSESLPGG
110 120 130 140 150
LSPPVEKYRY QDEEVLPSER ISPQVPNEVL GPELVHVSEK SLSEIENVHG
160 170 180 190 200
FVSHSHISPI KPTEAVPPSS PIVPVTPALP VPAESPVVLP STPQANPPPV
210 220 230 240 250
LVNTDSLETP TYVNGTDADY EYEEITLERG NSGLGFSIAG GTDNPHIGDD
260 270 280 290 300
SSIFITKIIT GGAAAQDGRL RVNDCILRVN EADVRDVTHS KAVEALKEAG
310 320 330 340 350
SIVRLYVKRR KAFRKNHEIK LIKGPKGLGF SIAGGVGNQH IPGDNSIYVT
360 370 380 390 400
KIIEGGAAHK DGKLQIGDKL LAVNSVCLEE VTHEEAVTAL KNTSDFVYLK
410 420 430 440 450
AAKPTSMYIN DGYAPPDITN SSSQSVDNHV SPSSYLGQTP ASPARYSPIS
460 470 480 490 500
KAVLGDDEIT REPRKVVLHR GSTGLGFNIV GGEDGEGIFI SFILAGGPAD
510 520 530 540 550
LSGELRKGDR IISVNSVDLR AASHEQAAAA LKNAGQAVTI VAQYRPEEYS
560 570 580 590 600
RFEAKIHDLR ETMMNSSVSS GSGSLRTSQK RSLYVRALFD YDKTKDSGLP
610 620 630 640 650
SQGLNFKFGD ILHVINASDD EWWQARQVTP DGESDEVGVI PSKRRVEKKE
660 670 680 690 700
RARLKTVKFN SKTRGDKGEI PDDMGSKGLK HVTSNASDSE SSYHEYGCSK
710 720 730 740 750
GGQEEYVLSY EPVNQQEVNY TRPVIILGPM KDRVNDDLIS EFPDKFGSCV
760 770 780 790 800
PHTTRPKRDY EVDGRDYHFV TSREQMEKDI QEHKFIEAGQ YNNHLYGTSV
810 820 830 840 850
QSVRAVAEKG KHCILDVSGN AIKRLQIAQL YPISIFIKPK SMENIMEMNK
860 870 880 890 900
RLTDEQARKT FERAVRLEQE FTEHFTAIVQ GDTLEDIYNQ VKQIIEEQSG
910
PYIWVPAKEK L
Length:911
Mass (Da):100,571
Last modified:November 1, 1997 - v1
Checksum:i18CEBD31DD0CAF8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14950 mRNA. Translation: AAA79976.1.
PIRiI56552.
RefSeqiNP_036920.1. NM_012788.1.
UniGeneiRn.89331.

Genome annotation databases

GeneIDi25252.
KEGGirno:25252.
UCSCiRGD:2505. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14950 mRNA. Translation: AAA79976.1.
PIRiI56552.
RefSeqiNP_036920.1. NM_012788.1.
UniGeneiRn.89331.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RSONMR-A/C4-63[»]
1ZOKNMR-A221-313[»]
2AWUX-ray2.44A/B314-409[»]
2AWWX-ray2.21A/B314-409[»]
2AWXX-ray1.80A/B314-409[»]
2G2LX-ray2.35A/B314-409[»]
2I0IX-ray2.80A/B/C459-543[»]
2I0LX-ray2.31A/B318-401[»]
3UATX-ray2.70A578-911[»]
ProteinModelPortaliQ62696.
SMRiQ62696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247292. 16 interactors.
DIPiDIP-31527N.
IntActiQ62696. 27 interactors.
MINTiMINT-93379.
STRINGi10116.ENSRNOP00000055672.

PTM databases

iPTMnetiQ62696.
PhosphoSitePlusiQ62696.
SwissPalmiQ62696.

Proteomic databases

PaxDbiQ62696.
PRIDEiQ62696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25252.
KEGGirno:25252.
UCSCiRGD:2505. rat.

Organism-specific databases

CTDi1739.
RGDi2505. Dlg1.

Phylogenomic databases

eggNOGiKOG0708. Eukaryota.
COG0194. LUCA.
HOGENOMiHOG000232102.
HOVERGENiHBG107814.
InParanoidiQ62696.
KOiK12076.
PhylomeDBiQ62696.

Enzyme and pathway databases

BRENDAi2.7.4.8. 5301.

Miscellaneous databases

EvolutionaryTraceiQ62696.
PROiQ62696.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR016313. DLG1.
IPR019590. DLG1_PEST_dom.
IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR015143. L27_1.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF09058. L27_1. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PIRSFiPIRSF001741. MAGUK_DLGH. 1 hit.
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 1 hit.
SM01277. MAGUK_N_PEST. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 1 hit.
SSF50044. SSF50044. 2 hits.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLG1_RAT
AccessioniPrimary (citable) accession number: Q62696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.